LOCUS       RG207268                8384 bp    DNA     circular  SYN 
DEFINITION  DENND6A (tGFP-tagged) - Human family with sequence similarity 116, member A (FAM116A)
ACCESSION   NM_152678
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2865..3569 
                     /label="GFP"  
     misc_feature    3616..4201 
                     /label="PolyA signal"  
     misc_feature    6293..6938 
                     /label="ColE1"  
     misc_feature    4655..5449 
                     /label="Neo"  
     misc_feature    4286..4588 
                     /label="SV40 ori"  
     misc_feature    6953..7813 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2844..2850 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2844 
                     /label="DENND6A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTTTGAGG GGCCCTGCGG GCTTGGGGCC CGGCTCTCGA AGGCCGTTGG 
     1081 ACGAAGCGGT GGCAGGGGCC GAGGGCCGCG AGGCGCCGGC CCTTGTGGCG GCGGGAGGCG 
     1141 CGCCAGAGGA CGATGAAGAG GACGATGGCC GTGGCCGGGG CCTGCTGCGC TGGGACAGCT 
     1201 TCTCCGCCTG GCTGCACTGC GTGTGTGTGG TGGGCTTCGA CCTGGAGCTG GGCCAGGCCG 
     1261 TGGAGGTAAT TTATCCTCAG CATTCCAAAC TTACTGACAG AGAAAAAACC AATATTTGCT 
     1321 ATTTGTCTTT TCCAGATTCA AATTCAGGTT GTCTTGGAGA TACCCAGTTT TGTTTTAGAT 
     1381 TTCGACAGTC TTCTGGGAGG AGGGTGTCGC TGCATTGTCT CCTGGATCAA TTTGACAAAG 
     1441 ATTTACCAGT TTACTTAAAG AAGGATCCTG CTTATTTTTA TGGATATGTG TATTTCCGAC 
     1501 AAGTTCGAGA TAAAACTCTA AAAAGAGGCT ACTTTCAGAA GTCCTTGGTT TTGATCAGCA 
     1561 AACTACCTTA TATTCATTTT TTTCACACTG TGCTCAAACA GATAGCACCA GAGTATTTTG 
     1621 AAAAGAATGA ACCTTATTTG GAAGCAGCTT GTAATGATGT TGATCGATGG CCTGCCCCAG 
     1681 TGCCAGGGAA AACATTACAC CTGCCAATCA TGGGGGTGGT AATGAAGGTA CGGATTCCCA 
     1741 CATGTCATGA CAAGCCTGGG ACAACTCAAA TAGTGCAGTT AACTCAGCAG GTGGACACAA 
     1801 ATATATCTGT TATTTTACCT ACTGTTCATG AGGTGGATAT TTTCAGGTGT TTCTGCCCAG 
     1861 TTTTCCTTCA TAGTCAGATG CTCTGGGAGC TGGTGCTGTT GGGGGAGCCC CTTGTGGTTA 
     1921 TGGCGCCATC ACCATCGGAA TCATCAGAGA CTGTATTGGC ACTTGTTAAC TGTATTTCTC 
     1981 CATTAAAGTA CTTCAGTGAT TTCCGACCTT ATTTCACTAT TCATGATAGT GAATTCAAAG 
     2041 AATATACTAC CCGTACGCAA GCTCCGCCCT CAGTTATATT AGGAGTAACC AACCCTTTTT 
     2101 TTGCTAAGAC ACTCCAGCAC TGGCCACACA TTATTCGAAT AGGAGACCTT AAACCTACAG 
     2161 GTGAAATTCC TAAGCAGGTT AAAGTGAAAA AACTGAAGAA TCTAAAGACT CTGGATTCCA 
     2221 AACCTGGAGT TTATACTTCA TATAAGCCAT ATTTAAATAG AGATGAAGAG ATCATAAAAC 
     2281 AATTACAGAA GGGTGTACAA CAGAAACGTC CTTCTGAGGC TCAAAGTGTT ATTCTTCGAC 
     2341 GCTATTTTTT GGAACTGACA CAAAGTTTCA TCATTCCATT AGAAAGATAT GTGGCAAGCT 
     2401 TGATGCCTTT GCAGAAAAGT ATTTCCCCAT GGAAGAGTCC ACCTCAATTA AGACAGTTTC 
     2461 TTCCAGAAGA ATTTATGAAA ACACTTGAGA AAACAGGACC TCAGCTAACC TCTAGAATAA 
     2521 AAGGCGATTG GATTGGACTT TACCGGCATT TCCTAAAGTC TCCAAATTTT GATGGCTGGT 
     2581 TTAAGACCCG GAGGAAGGAA ATGACCCAAA AATTGGAGGC ACTCCATCTA GAAGCTCTTT 
     2641 GTGAAGAGGA CTTACTTCTC TGGATCCAGA AACACACAGA AGTAGAAACA GTAGACCTTG 
     2701 TCTTGAAGCT GAAAAATAAG CTGTTGCAGG CTGATCGAGA GCACTTACCT GTGAAACCTG 
     2761 ACACTATGGA AAAGTTACGG ACACACATAG ATGCCATTAT CTTAGCATTG CCAGAGGACT 
     2821 TGCAAGGCAT ACTGCTCAAA ACGGGCATGA CAACGCGTAC GCGGCCGCTC GAGATGGAGA 
     2881 GCGACGAGAG CGGCCTGCCC GCCATGGAGA TCGAGTGCCG CATCACCGGC ACCCTGAACG 
     2941 GCGTGGAGTT CGAGCTGGTG GGCGGCGGAG AGGGCACCCC CGAGCAGGGC CGCATGACCA 
     3001 ACAAGATGAA GAGCACCAAA GGCGCCCTGA CCTTCAGCCC CTACCTGCTG AGCCACGTGA 
     3061 TGGGCTACGG CTTCTACCAC TTCGGCACCT ACCCCAGCGG CTACGAGAAC CCCTTCCTGC 
     3121 ACGCCATCAA CAACGGCGGC TACACCAACA CCCGCATCGA GAAGTACGAG GACGGCGGCG 
     3181 TGCTGCACGT GAGCTTCAGC TACCGCTACG AGGCCGGCCG CGTGATCGGC GACTTCAAGG 
     3241 TGATGGGCAC CGGCTTCCCC GAGGACAGCG TGATCTTCAC CGACAAGATC ATCCGCAGCA 
     3301 ACGCCACCGT GGAGCACCTG CACCCCATGG GCGATAACGA TCTGGATGGC AGCTTCACCC 
     3361 GCACCTTCAG CCTGCGCGAC GGCGGCTACT ACAGCTCCGT GGTGGACAGC CACATGCACT 
     3421 TCAAGAGCGC CATCCACCCC AGCATCCTGC AGAACGGGGG CCCCATGTTC GCCTTCCGCC 
     3481 GCGTGGAGGA GGATCACAGC AACACCGAGC TGGGCATCGT GGAGTACCAG CACGCCTTCA 
     3541 AGACCCCGGA TGCAGATGCC GGTGAAGAAA GAGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     3601 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     3661 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     3721 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     3781 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     3841 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     3901 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     3961 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4021 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4081 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4141 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4201 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCTTGG 
     4261 CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 
     4321 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA 
     4381 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA 
     4441 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA 
     4501 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG 
     4561 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA 
     4621 TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT 
     4681 GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA 
     4741 CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG 
     4801 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG 
     4861 CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT 
     4921 GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT 
     4981 CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG 
     5041 CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT 
     5101 ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC 
     5161 GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC 
     5221 GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA 
     5281 TTCATCGACT GTGGCCGGCT GGGTGTGGCC GACCGCTATC AGGACATAGC GTTGGCTACC 
     5341 CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT 
     5401 ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA 
     5461 GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT 
     5521 TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG 
     5581 GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT 
     5641 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG 
     5701 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG 
     5761 TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG 
     5821 TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA 
     5881 AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG 
     5941 CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA 
     6001 GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG 
     6061 TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG 
     6121 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC 
     6181 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA 
     6241 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT 
     6301 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC 
     6361 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC 
     6421 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC 
     6481 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT 
     6541 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG 
     6601 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA 
     6661 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA 
     6721 AACAAACCAC CGCTGGTAGC GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA 
     6781 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA 
     6841 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT 
     6901 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA 
     6961 GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA 
     7021 TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC 
     7081 CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA 
     7141 ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC 
     7201 AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA 
     7261 ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT 
     7321 TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG 
     7381 CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC 
     7441 TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT 
     7501 CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT 
     7561 GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC 
     7621 TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT 
     7681 CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA 
     7741 GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA 
     7801 CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG 
     7861 GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG 
     7921 TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG 
     7981 CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG 
     8041 CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC 
     8101 TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA 
     8161 AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC 
     8221 CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC 
     8281 TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT 
     8341 GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//