LOCUS       RG206228                8957 bp    DNA     circular  SYN 
DEFINITION  BEND2 (tGFP-tagged) - Human BEN domain containing 2 (BEND2), transcript variant 1
ACCESSION   NM_153346
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3438..4142 
                     /label="GFP"  
     misc_feature    4189..4774 
                     /label="PolyA signal"  
     misc_feature    6866..7511 
                     /label="ColE1"  
     misc_feature    5228..6022 
                     /label="Neo"  
     misc_feature    4859..5161 
                     /label="SV40 ori"  
     misc_feature    7526..8386 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3417..3423 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3417 
                     /label="BEND2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCAGAGAGG ACCCAGGAAC AGGATTTTGT TATTATAACT GTCGACGACA 
     1081 GTGATGATAA CAATGATTGC AGTATTGAGA TGGTGGAAGT TTCTGAAACA GCAGATAATT 
     1141 CCACTAATGA CATAGCAGAT GATTCCACTT ATGTCACAGC AGATAATCCC ACTGATGACA 
     1201 CAGCCACACA ACCAAATTTT CCAGGCGGCA ATGATGGCCA TCACCGTCCA CTCCAAATGT 
     1261 CATATGGGTC TGGCTCTGTC ACCCAGGCTG GAGTGCAGTG GCATGATCAC AGCTCACTGC 
     1321 AGCCTCAACC TCTTGGACTC AAGCAATTCT TCCACCTCAG CCTTCCCAGT AGCTGGGATG 
     1381 ACAGGCGCAC GCCACCATGC CCAGTAGCCC ATGGTGACCA AATAGTTTCA CAGATAAACC 
     1441 ACCCAGTACA TTTAAGAAGA TACAGTTACA ACTCAGAGGA AGTGGATTTT CCAAAAAGAG 
     1501 GAAGATTCTA TACTCCAGAG GTACAGTCTA GCATATCACC ACCAGCGGAA AGGCAGGAAA 
     1561 CCCATGCCTG GGCCAGCCCT GCTGTAACAT CTCTTGAGTC AGCAGCATGT CATGAACTGC 
     1621 AGGAAGCAGA CCTCAGTGAG AGTTTATCAT ATCCCAGAAT TGTCTCCTCA AGTTCATTAC 
     1681 AGCAGTATGT CGCACAAGGT GGCTCATTTC CTTGTTTCGG TATGCCATGG AACTTTATCA 
     1741 GTGGAGGTGC TGAAAGTACC AATGCTGTCA TCTCATTTGC CAATGCTACT ACAGCAGTAC 
     1801 CTATGGCAGT TCTGTCACGA AGAGAATCCA GTCTGGCAAA TAACCCTGGT GTGGTGAATT 
     1861 ACTCTGCTCT ACCAGAAAAT GAAAATGTGG GCCCAGGTAG AGCCTTGTCA TCTTTCTGCT 
     1921 TCCATCCCAA TTTGGAAATG CCAGAAAGAC CAGCAAACAG CAGTAAAAAC AGCACTGAGA 
     1981 CAGCGAATTA TCCAACTTTA ATGGGAAATT ACAATGGCCA AAATACTGCC TCTTTATCTG 
     2041 TCTTCATCCC TCCCTATTTT GCTGAGAAAA TTATACTTAC TGAAATGCCA GGAACAACAG 
     2101 AAACCAACGT GGAAAATAAC TCTCAGACAG TGTATTACCC AGCTTTATCG GGAAATACGA 
     2161 GTGCCCCATA TCCAGCCTCT TCATATCTTC CCATCACTTC TAATTTTGAA TCTGGCCCAC 
     2221 AAATGAGTTA TGGGACAATG AGTTACTCAA CTGAAATGAA AAATAACTGT GACCAAGATG 
     2281 ATGCTTCAGC ATCTGCCTGC CTCACTCCCG ATTTTGCACT GTTACCTCTG AATATTTTGG 
     2341 TAAAAGTAGA CACCAACACG GAAAACAGCG TCAACACAAT GAATCGCTCA ACTTTATTGG 
     2401 ACAGTGACAG TGGCCAGGAT TCTTCCTCAT CATCTGTCTG TATCCCTCCC AAGTATGGCT 
     2461 ATCTTGGTGA TCCAAAAAGA AATGTCAGAG TACTTAAAAT CCATTTGCTG GCCGTACAAA 
     2521 ATATGGCCAA ACCTAAGCAA GCAGCCTGCT ACTTGGTTCG TATTTTGTTC TCCAAGGAAA 
     2581 TCCTGATTAG CAGCTCAGTG GATATCCATT TGAAAGACAG CCAATCCCTC GACCCGAACA 
     2641 AAATGGCTGC ATTGAGAGAA TATCTGGCAA CAACTTTTCC CACCTGTGAT TTGCATGAAC 
     2701 ATGGAAAAGA CTGGCAGGAC TGTATTTCTG GTATCAATTC TATGATCTAC TGTTTATGTT 
     2761 CTGAAGGCAA GAGTACTCCA AAAACTGTTC GCAAAAATAA AAAACGTACC AACCGTGTTG 
     2821 CATCGGCATC TGCAGATAGA AATGACCAAA GGGGCAGAGA TGGTGGTGAA GGCTGTTCTT 
     2881 GGATGTTTCA GCCAATGAAT AACTCCAAAA TGAGAGTAAA GAGGAACTTG CAGCCAAACA 
     2941 GTAATGCTAT CCCTGAAGGA ATGCGAGAAC CTTCCACTGA TAATCCAGAG GAACCTGGTG 
     3001 AAGCATGGAG CTATTTTGGA AGACCATGGA GAAATATACG GATGCCATGT TCAGTACTGA 
     3061 CTTTGGCAAA AACTAAGTCT TGCGCAAGCC TGTCGGCTAG ATACCTTATT CAGAAACTCT 
     3121 TCACAAAAGA TGTCCTGGTC CAAAGTAACG TCTATGGCAA TCTGAAGCAT GGCCTGTGTG 
     3181 CCCTTGACCC CAATAAGATT AGTGCTCTCC GAGAGTTCCT CCAAGAAAAC TACCCAATTT 
     3241 GTGATCTCTC GGAAAATGGA AGAGACTGGA AGTCGTGTGT GACCTCCATC AACAGCGGTA 
     3301 TCCGTAGCCT TAGACATGAC GTCAGAAGGG CTGAAGCCAG GTCTCAGTCG CTTCCAGCAG 
     3361 TGACCCCTCC AGAGCTGGAG CAGGAGTCAA AGCCAGGAGA TCCCGACGCC ACTGACCCAA 
     3421 GCACCACGCG TACGCGGCCG CTCGAGATGG AGAGCGACGA GAGCGGCCTG CCCGCCATGG 
     3481 AGATCGAGTG CCGCATCACC GGCACCCTGA ACGGCGTGGA GTTCGAGCTG GTGGGCGGCG 
     3541 GAGAGGGCAC CCCCGAGCAG GGCCGCATGA CCAACAAGAT GAAGAGCACC AAAGGCGCCC 
     3601 TGACCTTCAG CCCCTACCTG CTGAGCCACG TGATGGGCTA CGGCTTCTAC CACTTCGGCA 
     3661 CCTACCCCAG CGGCTACGAG AACCCCTTCC TGCACGCCAT CAACAACGGC GGCTACACCA 
     3721 ACACCCGCAT CGAGAAGTAC GAGGACGGCG GCGTGCTGCA CGTGAGCTTC AGCTACCGCT 
     3781 ACGAGGCCGG CCGCGTGATC GGCGACTTCA AGGTGATGGG CACCGGCTTC CCCGAGGACA 
     3841 GCGTGATCTT CACCGACAAG ATCATCCGCA GCAACGCCAC CGTGGAGCAC CTGCACCCCA 
     3901 TGGGCGATAA CGATCTGGAT GGCAGCTTCA CCCGCACCTT CAGCCTGCGC GACGGCGGCT 
     3961 ACTACAGCTC CGTGGTGGAC AGCCACATGC ACTTCAAGAG CGCCATCCAC CCCAGCATCC 
     4021 TGCAGAACGG GGGCCCCATG TTCGCCTTCC GCCGCGTGGA GGAGGATCAC AGCAACACCG 
     4081 AGCTGGGCAT CGTGGAGTAC CAGCACGCCT TCAAGACCCC GGATGCAGAT GCCGGTGAAG 
     4141 AAAGAGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4201 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4261 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4321 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4381 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4441 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4501 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4561 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4621 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4681 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4741 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4801 ATGCCTGTTG AATTGGGTAC GCGGCCAGCT TGGCTGTGGA ATGTGTGTCA GTTAGGGTGT 
     4861 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 
     4921 GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 
     4981 CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG 
     5041 CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC 
     5101 GAGGCCGCCT CGGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA 
     5161 GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA 
     5221 GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT 
     5281 TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC 
     5341 GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC 
     5401 GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC 
     5461 GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG 
     5521 GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC 
     5581 ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC 
     5641 CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT 
     5701 CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC 
     5761 AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG 
     5821 AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG 
     5881 GCCGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC 
     5941 GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC 
     6001 GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG 
     6061 ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA 
     6121 GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC 
     6181 TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT 
     6241 AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG 
     6301 GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA 
     6361 GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC 
     6421 CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT 
     6481 AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC 
     6541 AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT 
     6601 CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG 
     6661 CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA 
     6721 TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT 
     6781 TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC 
     6841 GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT 
     6901 CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG 
     6961 TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA 
     7021 AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT 
     7081 ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA 
     7141 ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA 
     7201 ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT 
     7261 TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTTTTT 
     7321 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT 
     7381 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA 
     7441 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA 
     7501 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC 
     7561 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA 
     7621 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC 
     7681 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA 
     7741 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA 
     7801 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG 
     7861 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC 
     7921 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG 
     7981 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT 
     8041 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT 
     8101 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA 
     8161 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC 
     8221 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC 
     8281 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA 
     8341 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT 
     8401 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT 
     8461 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC 
     8521 CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG 
     8581 TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC 
     8641 TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC 
     8701 GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA 
     8761 GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA 
     8821 ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG 
     8881 ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA 
     8941 AATTTAACGC GAATTTT
//