LOCUS       RG205821                8801 bp    DNA     circular  SYN 
DEFINITION  TPX2 (tGFP-tagged) - Human TPX2, microtubule-associated, homolog (Xenopus laevis) (TPX2)
ACCESSION   NM_012112
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3282..3986 
                     /label="GFP"  
     misc_feature    4033..4618 
                     /label="PolyA signal"  
     misc_feature    6710..7355 
                     /label="ColE1"  
     misc_feature    5072..5866 
                     /label="Neo"  
     misc_feature    4703..5005 
                     /label="SV40 ori"  
     misc_feature    7370..8230 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3261..3267 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3261 
                     /label="TPX2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCACAAGTT AAAAGCTCTT ATTCCTATGA TGCCCCCTCG GATTTCATCA 
     1081 ATTTTTCATC CTTGGATGAT GAAGGAGATA CTCAAAACAT AGATTCATGG TTTGAGGAGA 
     1141 AGGCCAATTT GGAGAATAAG TTACTGGGGA AGAATGGAAC TGGAGGGCTT TTTCAGGGCA 
     1201 AAACTCCTTT GAGAAAGGCT AATCTTCAGC AAGCTATTGT CACACCTTTG AAACCAGTTG 
     1261 ACAACACTTA CTACAAAGAG GCAGAAAAAG AAAATCTTGT GGAACAATCC ATTCCGTCAA 
     1321 ATGCTTGTTC TTCCCTGGAA GTTGAGGCAG CCATATCAAG AAAAACTCCA GCCCAGCCTC 
     1381 AGAGAAGATC TCTTAGGCTT TCTGCTCAGA AGGATTTGGA ACAGAAAGAA AAGCATCATG 
     1441 TAAAAATGAA AGCCAAGAGA TGTGCCACTC CTGTAATCAT CGATGAAATT CTACCCTCTA 
     1501 AGAAAATGAA AGTTTCTAAC AACAAAAAGA AGCCAGAGGA AGAAGGCAGT GCTCATCAAG 
     1561 ATACTGCTGA AAAGAATGCA TCTTCCCCAG AGAAAGCCAA GGGTAGACAT ACTGTGCCTT 
     1621 GTATGCCACC TGCAAAGCAG AAGTTTCTAA AAAGTACTGA GGAGCAAGAG CTGGAGAAGA 
     1681 GTATGAAAAT GCAGCAAGAG GTGGTGGAGA TGCGGAAAAA GAATGAAGAA TTCAAGAAAC 
     1741 TTGCTCTGGC TGGAATAGGG CAACCTGTGA AGAAATCAGT GAGCCAGGTC ACCAAATCAG 
     1801 TTGACTTCCA CTTCCGCACA GATGAGCGAA TCAAACAACA TCCTAAGAAC CAGGAGGAAT 
     1861 ATAAGGAAGT GAACTTTACA TCTGAACTAC GAAAGCATCC TTCATCTCCT GCCCGAGTGA 
     1921 CTAAGGGATG TACCATTGTT AAGCCTTTCA ACCTGTCCCA AGGAAAGAAA AGAACATTTG 
     1981 ATGAAACAGT TTCTACATAT GTGCCCCTTG CACAGCAAGT TGAAGACTTC CATAAACGAA 
     2041 CCCCTAACAG ATATCATTTG AGGAGCAAGA AGGATGATAT TAACCTGTTA CCCTCCAAAT 
     2101 CTTCTGTGAC CAAGATTTGC AGAGACCCAC AGACTCCTGT ACTGCAAACC AAACACCGTG 
     2161 CACGGGCTGT GACCTGCAAA AGTACAGCAG AGCTGGAGGC TGAGGAGCTC GAGAAATTGC 
     2221 AACAATACAA ATTCAAAGCA CGTGAACTTG ATCCCAGAAT ACTTGAAGGT GGGCCCATCT 
     2281 TGCCCAAGAA ACCACCTGTG AAACCACCCA CCGAGCCTAT TGGCTTTGAT TTGGAAATTG 
     2341 AGAAAAGAAT CCAGGAGCGA GAATCAAAGA AGAAAACAGA GGATGAACAC TTTGAATTTC 
     2401 ATTCCAGACC TTGCCCTACT AAGATTTTGG AAGATGTTGT GGGTGTTCCT GAAAAGAAGG 
     2461 TACTTCCAAT CACCGTCCCC AAGTCACCAG CCTTTGCATT GAAGAACAGA ATTCGAATGC 
     2521 CCACCAAAGA AGATGAGGAA GAGGACGAAC CGGTAGTGAT AAAAGCTCAA CCTGTGCCAC 
     2581 ATTATGGGGT GCCTTTTAAG CCCCAAATCC CAGAGGCAAG AACTGTGGAA ATATGCCCTT 
     2641 TCTCGTTTGA TTCTCGAGAC AAAGAACGTC AGTTACAGAA GGAGAAGAAA ATAAAAGAAC 
     2701 TGCAGAAAGG GGAGGTGCCC AAGTTCAAGG CACTTCCCTT GCCTCATTTT GACACCATTA 
     2761 ACCTGCCAGA GAAGAAGGTA AAGAATGTGA CCCAGATTGA ACCTTTCTGC TTGGAGACTG 
     2821 ACAGAAGAGG TGCTCTGAAG GCACAGACTT GGAAGCACCA GCTGGAAGAA GAACTGAGAC 
     2881 AGCAGAAAGA AGCAGCTTGT TTCAAGGCTC GTCCAAACAC CGTCATCTCT CAGGAGCCCT 
     2941 TTGTTCCCAA GAAAGAGAAG AAATCAGTTG CTGAGGGCCT TTCTGGTTCT CTAGTTCAGG 
     3001 AACCTTTTCA GCTGGCTACT GAGAAGAGAG CCAAAGAGCG GCAGGAGCTG GAGAAGAGAA 
     3061 TGGCTGAGGT AGAAGCCCAG AAAGCCCAGC AGTTGGAGGA GGCCAGACTA CAGGAGGAAG 
     3121 AGCAGAAAAA AGAGGAGCTG GCCAGGCTAC GGAGAGAACT GGTGCATAAG GCAAATCCAA 
     3181 TACGCAAGTA CCAGGGTCTG GAGATAAAGT CAAGTGACCA GCCTCTGACT GTGCCTGTAT 
     3241 CTCCCAAATT CTCCACTCGA TTCCACTGCA CGCGTACGCG GCCGCTCGAG ATGGAGAGCG 
     3301 ACGAGAGCGG CCTGCCCGCC ATGGAGATCG AGTGCCGCAT CACCGGCACC CTGAACGGCG 
     3361 TGGAGTTCGA GCTGGTGGGC GGCGGAGAGG GCACCCCCGA GCAGGGCCGC ATGACCAACA 
     3421 AGATGAAGAG CACCAAAGGC GCCCTGACCT TCAGCCCCTA CCTGCTGAGC CACGTGATGG 
     3481 GCTACGGCTT CTACCACTTC GGCACCTACC CCAGCGGCTA CGAGAACCCC TTCCTGCACG 
     3541 CCATCAACAA CGGCGGCTAC ACCAACACCC GCATCGAGAA GTACGAGGAC GGCGGCGTGC 
     3601 TGCACGTGAG CTTCAGCTAC CGCTACGAGG CCGGCCGCGT GATCGGCGAC TTCAAGGTGA 
     3661 TGGGCACCGG CTTCCCCGAG GACAGCGTGA TCTTCACCGA CAAGATCATC CGCAGCAACG 
     3721 CCACCGTGGA GCACCTGCAC CCCATGGGCG ATAACGATCT GGATGGCAGC TTCACCCGCA 
     3781 CCTTCAGCCT GCGCGACGGC GGCTACTACA GCTCCGTGGT GGACAGCCAC ATGCACTTCA 
     3841 AGAGCGCCAT CCACCCCAGC ATCCTGCAGA ACGGGGGCCC CATGTTCGCC TTCCGCCGCG 
     3901 TGGAGGAGGA TCACAGCAAC ACCGAGCTGG GCATCGTGGA GTACCAGCAC GCCTTCAAGA 
     3961 CCCCGGATGC AGATGCCGGT GAAGAAAGAG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4021 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4081 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4141 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4201 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4261 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4321 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4381 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4441 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4501 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4561 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     4621 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCTTGGCTG 
     4681 TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 
     4741 CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 
     4801 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT 
     4861 CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA 
     4921 ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG 
     4981 TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC 
     5041 ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA 
     5101 TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA 
     5161 CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT 
     5221 CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG 
     5281 CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA 
     5341 GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC 
     5401 CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT 
     5461 GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT 
     5521 CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG 
     5581 CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG 
     5641 ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC 
     5701 ATCGACTGTG GCCGGCTGGG TGTGGCCGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT 
     5761 GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC 
     5821 GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG 
     5881 GGACTCTGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG 
     5941 ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT 
     6001 GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA 
     6061 TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT 
     6121 TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT 
     6181 GTATACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT 
     6241 GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG 
     6301 CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT 
     6361 TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG 
     6421 GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG 
     6481 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT 
     6541 CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA 
     6601 AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA 
     6661 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC 
     6721 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT 
     6781 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA 
     6841 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG 
     6901 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT 
     6961 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA 
     7021 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT 
     7081 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC 
     7141 AAACCACCGC TGGTAGCGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG 
     7201 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT 
     7261 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA 
     7321 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT 
     7381 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG 
     7441 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA 
     7501 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC 
     7561 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT 
     7621 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG 
     7681 TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA 
     7741 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG 
     7801 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA 
     7861 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG 
     7921 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT 
     7981 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA 
     8041 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA 
     8101 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG 
     8161 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC 
     8221 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT 
     8281 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC 
     8341 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG 
     8401 CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC 
     8461 CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA 
     8521 ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC 
     8581 TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT 
     8641 TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA 
     8701 ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT 
     8761 TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//