LOCUS       RG205643                9212 bp    DNA     circular  SYN 
DEFINITION  CHERP (tGFP-tagged) - Human calcium homeostasis endoplasmic reticulum protein (CHERP)
ACCESSION   NM_006387
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3693..4397 
                     /label="GFP"  
     misc_feature    4444..5029 
                     /label="PolyA signal"  
     misc_feature    7121..7766 
                     /label="ColE1"  
     misc_feature    5483..6277 
                     /label="Neo"  
     misc_feature    5114..5416 
                     /label="SV40 ori"  
     misc_feature    7781..8641 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3672..3678 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3672 
                     /label="CHERP"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACTATGGAG AAGCAGAAGG ACAACCCCAA ATTCTCGTTT CTTTTCGGAG 
     1081 GCGAATTCTA CAGTTACTAC AAGTGCAAGC TGGCGCTGGA GCAGCAGCAG CTCATCTGCA 
     1141 AGCAGCAGAC CCCGGAGCTG GAGCCAGCCG CCACCATGCC ACCCCTGCCA CAGCCCCCGC 
     1201 TGGCCCCCGC CGCGCCCATC CCGCCGGCCC AGGGCGCGCC ATCCATGGAC GAGCTCATCC 
     1261 AGCAGAGCCA GTGGAACCTC CAGCAGCAGG AGCAGCACTT GCTGGCGCTC AGACAGGAGC 
     1321 AAGTGACAGC GGCCGTGGCC CACGCGGTGG AGCAGCAGAT GCAGAAGCTT CTGGAGGAGA 
     1381 CCCAGCTAGA CATGAACGAG TTTGACAACC TCCTGCAGCC CATCATCGAC ACGTGCACCA 
     1441 AGGACGCCAT CTCGGCCGGG AAGAACTGGA TGTTCAGCAA TGCCAAGTCC CCGCCGCACT 
     1501 GTGAGCTGAT GGCCGGCCAC CTCCGGAACC GCATCACGGC TGATGGGGCA CACTTCGAGC 
     1561 TGCGGCTGCA CCTCATCTAC CTGATCAATG ACGTGCTGCA CCACTGCCAG CGCAAGCAGG 
     1621 CCCGGGAGCT GCTGGCCGCC CTGCAGAAGG TCGTGGTGCC CATCTACTGC ACCAGCTTCT 
     1681 TGGCCGTGGA GGAAGACAAG CAGCAGAAGA TCGCCCGGCT CCTGCAGCTC TGGGAGAAAA 
     1741 ACGGCTACTT CGATGACTCC ATCATTCAGC AGCTACAGAG CCCAGCCCTG GGGCTTGGTC 
     1801 AGTACCAGGC CACCCTCATC AACGAGTACT CCTCAGTGGT CCAGCCGGTG CAGCTGGCCT 
     1861 TCCAGCAGCA GATCCAGACC CTCAAGACGC AGCACGAGGA GTTTGTCACC AGCCTGGCCC 
     1921 AGCAGCAGCA GCAGCAGCAA CAGCAGCAGC AGCAGCTCCA GATGCCGCAG ATGGAGGCTG 
     1981 AAGTCAAGGC CACGCCTCCA CCGCCTGCTC CACCCCCGGC CCCAGCACCT GCCCCTGCCA 
     2041 TCCCGCCCAC CACCCAGCCT GATGACAGCA AGCCTCCCAT CCAGATGCCT GGCTCTTCAG 
     2101 AGTACGAAGC TCCAGGAGGG GTCCAGGATC CTGCAGCTGC CGGCCCCCGG GGCCCCGGGC 
     2161 CACACGACCA GATCCCACCA AACAAGCCCC CTTGGTTTGA CCAGCCTCAC CCCGTGGCTC 
     2221 CTTGGGGCCA GCAGCAGCCG CCAGAGCAGC CACCCTACCC GCACCACCAG GGCGGCCCAC 
     2281 CCCACTGCCC CCCCTGGAAC AACAGCCATG AGGGCATGTG GGGCGAGCAG CGCGGTGACC 
     2341 CCGGCTGGAA CGGCCAGCGC GACGCGCCCT GGAACAACCA GCCCGACGCC GCCTGGAACA 
     2401 GCCAGTTCGA GGGCCCCTGG AACAGCCAGC ACGAGCAGCC GCCCTGGGGC GGGGGCCAGC 
     2461 GCGAGCCACC CTTCCGCATG CAGCGGCCCC CACACTTCCG GGGGCCCTTC CCGCCCCACC 
     2521 AGCAGCACCC GCAGTTCAAC CAGCCTCCGC ACCCCCACAA CTTCAACCGC TTCCCGCCCC 
     2581 GCTTCATGCA GGACGACTTC CCGCCACGGC ACCCCTTCGA GCGGCCGCCC TATCCCCACC 
     2641 GCTTCGACTA CCCCCAGGGG GACTTCCCTG CCGAAATGGG GCCCCCTCAC CACCACCCTG 
     2701 GCCACCGCAT GCCTCATCCT GGCATCAACG AGCACCCGCC TTGGGCTGGA CCCCAGCACC 
     2761 CTGACTTCGG CCCTCCCCCC CATGGCTTCA ACGGGCAGCC CCCACACATG CGGCGACAGG 
     2821 GCCCGCCCCA CATCAACCAC GATGACCCCA GCCTGGTCCC CAATGTGCCC TACTTCGATC 
     2881 TCCCTGCTGG GCTGATGGCC CCCCTCGTGA AGCTGGAAGA TCACGAGTAC AAGCCTTTGG 
     2941 ACCCTAAAGA CATCCGCCTC CCACCCCCCA TGCCGCCCAG CGAGAGGCTG CTGGCTGCAG 
     3001 TGGAGGCCTT CTACAGCCCC CCGTCCCACG ACAGGCCCAG GAACAGTGAA GGCTGGGAGC 
     3061 AGAACGGCCT CTATGAGTTC TTCCGAGCAA AAATGCGGGC CCGGCGGAGG AAAGGCCAGG 
     3121 AGAAGAGGAA CAGCGGACCC TCGAGGTCTC GGAGCAGATC CAAGAGTCGA GGGCGTTCTT 
     3181 CCTCCCGCTC CAACTCAAGA TCCTCCAAGT CTTCAGGCTC GTACTCAAGG TCAAGGTCGC 
     3241 GCTCCTGCTC CCGTTCCTAC TCCCGCTCCA GATCTAGAAG TCGGAGCAGG TCGCGCTCCT 
     3301 CCAGAAGCCG CTCCCGGTCC CAGTCGCGGT CCCGGTCCAA GTCGTACTCC CCAGGAAGAA 
     3361 GACGCCGGTC ACGGTCCAGG AGCCCCACCC CGCCTTCCTC TGCTGGTCTG GGTTCTAATT 
     3421 CGGCGCCTCC CATTCCTGAC TCAAGGCTCG GAGAAGAGAA CAAAGGCCAT CAGATGCTGG 
     3481 TGAAGATGGG CTGGAGCGGC TCAGGCGGCC TCGGTGCGAA GGAGCAAGGG ATCCAGGACC 
     3541 CCATCAAGGG CGGGGACGTC CGGGATAAGT GGGACCAGTA TAAAGGCGTG GGCGTGGCTC 
     3601 TGGATGACCC CTATGAGAAC TACCGCAGGA ACAAGAGCTA CTCCTTCATC GCCCGCATGA 
     3661 AGGCCAGGGA CGAGTGTAAG ACGCGTACGC GGCCGCTCGA GATGGAGAGC GACGAGAGCG 
     3721 GCCTGCCCGC CATGGAGATC GAGTGCCGCA TCACCGGCAC CCTGAACGGC GTGGAGTTCG 
     3781 AGCTGGTGGG CGGCGGAGAG GGCACCCCCG AGCAGGGCCG CATGACCAAC AAGATGAAGA 
     3841 GCACCAAAGG CGCCCTGACC TTCAGCCCCT ACCTGCTGAG CCACGTGATG GGCTACGGCT 
     3901 TCTACCACTT CGGCACCTAC CCCAGCGGCT ACGAGAACCC CTTCCTGCAC GCCATCAACA 
     3961 ACGGCGGCTA CACCAACACC CGCATCGAGA AGTACGAGGA CGGCGGCGTG CTGCACGTGA 
     4021 GCTTCAGCTA CCGCTACGAG GCCGGCCGCG TGATCGGCGA CTTCAAGGTG ATGGGCACCG 
     4081 GCTTCCCCGA GGACAGCGTG ATCTTCACCG ACAAGATCAT CCGCAGCAAC GCCACCGTGG 
     4141 AGCACCTGCA CCCCATGGGC GATAACGATC TGGATGGCAG CTTCACCCGC ACCTTCAGCC 
     4201 TGCGCGACGG CGGCTACTAC AGCTCCGTGG TGGACAGCCA CATGCACTTC AAGAGCGCCA 
     4261 TCCACCCCAG CATCCTGCAG AACGGGGGCC CCATGTTCGC CTTCCGCCGC GTGGAGGAGG 
     4321 ATCACAGCAA CACCGAGCTG GGCATCGTGG AGTACCAGCA CGCCTTCAAG ACCCCGGATG 
     4381 CAGATGCCGG TGAAGAAAGA GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4441 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4501 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4561 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4621 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4681 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4741 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4801 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4861 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4921 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4981 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5041 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCTTGGCT GTGGAATGTG 
     5101 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 
     5161 CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 
     5221 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC 
     5281 CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT 
     5341 ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC 
     5401 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA 
     5461 TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA 
     5521 GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC 
     5581 GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC 
     5641 AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG 
     5701 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG 
     5761 GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT 
     5821 GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT 
     5881 ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA 
     5941 GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA 
     6001 CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC 
     6061 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT 
     6121 GGCCGGCTGG GTGTGGCCGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT 
     6181 GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC 
     6241 GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG 
     6301 GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG 
     6361 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC 
     6421 TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT 
     6481 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC 
     6541 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT 
     6601 CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT 
     6661 ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG 
     6721 CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG 
     6781 GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC 
     6841 GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC 
     6901 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 
     6961 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 
     7021 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 
     7081 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 
     7141 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 
     7201 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 
     7261 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 
     7321 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 
     7381 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 
     7441 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC 
     7501 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 
     7561 CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG 
     7621 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG 
     7681 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT 
     7741 GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT 
     7801 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC 
     7861 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA 
     7921 TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG 
     7981 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT 
     8041 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA 
     8101 TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT 
     8161 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT 
     8221 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG 
     8281 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG 
     8341 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG 
     8401 CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA 
     8461 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT 
     8521 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT 
     8581 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT 
     8641 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA 
     8701 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT 
     8761 TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG 
     8821 TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT 
     8881 TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC 
     8941 TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG 
     9001 GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG 
     9061 AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT 
     9121 CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG 
     9181 AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//