LOCUS       RG205557                8438 bp    DNA     circular  SYN 
DEFINITION  TKTL2 (tGFP-tagged) - Human transketolase-like 2 (TKTL2)
ACCESSION   NM_032136
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2919..3623 
                     /label="GFP"  
     misc_feature    3670..4255 
                     /label="PolyA signal"  
     misc_feature    6347..6992 
                     /label="ColE1"  
     misc_feature    4709..5503 
                     /label="Neo"  
     misc_feature    4340..4642 
                     /label="SV40 ori"  
     misc_feature    7007..7867 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2898..2904 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2898 
                     /label="TKTL2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGGCCAAC GACGCCAAGC CCGACGTGAA GACCGTGCAG GTGCTGCGGG 
     1081 ACACAGCCAA CCGCCTGCGG ATCCATTCCA TCAGGGCCAC GTGTGCCTCT GGTTCTGGCC 
     1141 AGCTCACGTC GTGCTGCAGT GCAGCGGAGG TCGTGTCTGT CCTCTTCTTC CACACGATGA 
     1201 AGTATAAACA GACAGACCCA GAACACCCGG ACAACGACCG GTTCATCCTC TCCAGGGGAC 
     1261 ATGCTGCTCC TATCCTCTAT GCTGCTTGGG TGGAGGTGGG TGACATCAGT GAATCTGACT 
     1321 TGCTGAACCT GAGGAAACTT CACAGCGACT TGGAGAGACA CCCTACCCCC CGATTGCCGT 
     1381 TTGTTGACGT GGCAACAGGG TCCCTAGGTC AGGGATTAGG TACTGCATGT GGAATGGCTT 
     1441 ATACTGGCAA GTACCTTGAC AAGGCCAGCT ACCGGGTGTT CTGCCTTATG GGAGATGGCG 
     1501 AATCCTCAGA AGGCTCTGTG TGGGAGGCTT TTGCTTTTGC CTCCCACTAC AACTTGGACA 
     1561 ATCTCGTGGC GGTCTTCGAC GTGAACCGCT TGGGACAAAG TGGCCCTGCA CCCCTTGAGC 
     1621 ATGGCGCAGA CATCTACCAG AATTGCTGTG AAGCCTTTGG ATGGAATACT TACTTAGTGG 
     1681 ATGGCCATGA TGTGGAGGCC TTGTGCCAAG CATTTTGGCA AGCAAGTCAA GTGAAGAACA 
     1741 AGCCTACTGC TATAGTTGCC AAGACCTTCA AAGGTCGGGG TATTCCAAAT ATTGAGGATG 
     1801 CAGAAAATTG GCATGGAAAG CCAGTGCCAA AAGAAAGAGC AGATGCAATT GTCAAATTAA 
     1861 TTGAGAGTCA GATACAGACC AATGAGAATC TCATACCAAA ATCGCCTGTG GAAGACTCAC 
     1921 CTCAAATAAG CGTCACAGAT ATAAAAATGA CCTCCCCACA TGCTTACAAA GTTGGTGACA 
     1981 AGATAGCTAC TCAGAAAACA TATGGTTTGG CTCTGGCTAA ACTGGGCCGT GCAAATGAAA 
     2041 GAGTTATTGT TCTGAGTGGT GACACGATGA ACTCCACCTT TTCTGAGATA TTCAGGAAAG 
     2101 AACACCCTGA GCGTTTCATA GAGTGTATTA TTGCTGAACA AAACATGGTA AGTGTGGCAC 
     2161 TAGGCTGTGC TACACGTGGT CGAACCATTG CTTTTGCTGG TGCTTTTGCT GCCTTTTTTA 
     2221 CTAGAGCATT CGATCAGCTC CGAATGGGAG CCATTTCTCA AGCCAATATC AACCTTATTG 
     2281 GTTCCCACTG TGGGGTATCC ACTGGAGAAG ATGGAGTCTC CCAGATGGCC CTGGAGGATC 
     2341 TAGCCATGTT CCGAAGCATT CCCAATTGTA CTGTTTTCTA TCCAAGTGAT GCCATCTCGA 
     2401 CAGAGCATGC TATTTATCTA GCCGCCAATA CCAAGGGAAT GTGCTTCATT CGAACCAGCC 
     2461 AACCAGAAAC TGCAGTTATT TATACCCCAC AAGAAAATTT TGAGATTGGC CAGGCCAAGG 
     2521 TGGTCCGCCA CGGTGTCAAT GATAAAGTCA CAGTAATTGG AGCTGGAGTT ACTCTCCATG 
     2581 AAGCCTTAGA AGCTGCTGAC CATCTTTCTC AACAAGGTAT TTCTGTCCGT GTCATCGACC 
     2641 CATTTACCAT TAAACCCCTG GATGCCGCCA CCATCATCTC CAGTGCAAAA GCCACAGGCG 
     2701 GCCGAGTTAT CACAGTGGAG GATCACTACA GGGAAGGTGG CATTGGAGAA GCTGTTTGTG 
     2761 CAGCTGTCTC CAGGGAGCCT GATATCCTTG TTCATCAACT GGCAGTGTCA GGAGTGCCTC 
     2821 AACGTGGGAA AACTAGTGAA TTGCTGGATA TGTTTGGAAT CAGTACCAGA CACATTATAG 
     2881 CAGCCGTAAC ACTTACTTTA ATGAAGACGC GTACGCGGCC GCTCGAGATG GAGAGCGACG 
     2941 AGAGCGGCCT GCCCGCCATG GAGATCGAGT GCCGCATCAC CGGCACCCTG AACGGCGTGG 
     3001 AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA CCCCCGAGCA GGGCCGCATG ACCAACAAGA 
     3061 TGAAGAGCAC CAAAGGCGCC CTGACCTTCA GCCCCTACCT GCTGAGCCAC GTGATGGGCT 
     3121 ACGGCTTCTA CCACTTCGGC ACCTACCCCA GCGGCTACGA GAACCCCTTC CTGCACGCCA 
     3181 TCAACAACGG CGGCTACACC AACACCCGCA TCGAGAAGTA CGAGGACGGC GGCGTGCTGC 
     3241 ACGTGAGCTT CAGCTACCGC TACGAGGCCG GCCGCGTGAT CGGCGACTTC AAGGTGATGG 
     3301 GCACCGGCTT CCCCGAGGAC AGCGTGATCT TCACCGACAA GATCATCCGC AGCAACGCCA 
     3361 CCGTGGAGCA CCTGCACCCC ATGGGCGATA ACGATCTGGA TGGCAGCTTC ACCCGCACCT 
     3421 TCAGCCTGCG CGACGGCGGC TACTACAGCT CCGTGGTGGA CAGCCACATG CACTTCAAGA 
     3481 GCGCCATCCA CCCCAGCATC CTGCAGAACG GGGGCCCCAT GTTCGCCTTC CGCCGCGTGG 
     3541 AGGAGGATCA CAGCAACACC GAGCTGGGCA TCGTGGAGTA CCAGCACGCC TTCAAGACCC 
     3601 CGGATGCAGA TGCCGGTGAA GAAAGAGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     3661 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     3721 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     3781 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     3841 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     3901 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     3961 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4021 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4081 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4141 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4201 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4261 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC TTGGCTGTGG 
     4321 AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 
     4381 AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 
     4441 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG 
     4501 CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT 
     4561 TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA 
     4621 GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT 
     4681 TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG 
     4741 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG 
     4801 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT 
     4861 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA 
     4921 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG 
     4981 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT 
     5041 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT 
     5101 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG 
     5161 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA 
     5221 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC 
     5281 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC 
     5341 GACTGTGGCC GGCTGGGTGT GGCCGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT 
     5401 ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC 
     5461 GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA 
     5521 CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT 
     5581 CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA 
     5641 TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG 
     5701 CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT 
     5761 TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA 
     5821 TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA 
     5881 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT 
     5941 GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC 
     6001 AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG 
     6061 GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC 
     6121 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG 
     6181 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA 
     6241 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC 
     6301 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC 
     6361 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG 
     6421 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT 
     6481 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC 
     6541 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC 
     6601 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG 
     6661 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG 
     6721 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA 
     6781 CCACCGCTGG TAGCGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT 
     6841 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC 
     6901 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT 
     6961 AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC 
     7021 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG 
     7081 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG 
     7141 CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC 
     7201 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA 
     7261 TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG 
     7321 TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT 
     7381 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA 
     7441 GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG 
     7501 TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA 
     7561 CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT 
     7621 GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA 
     7681 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT 
     7741 CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT 
     7801 CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA 
     7861 AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT 
     7921 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC 
     7981 GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG 
     8041 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT 
     8101 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC 
     8161 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG 
     8221 ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA 
     8281 CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC 
     8341 CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA 
     8401 AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//