LOCUS       RG205508                9581 bp    DNA     circular  SYN 
DEFINITION  PRPF4B (tGFP-tagged) - Human PRP4 pre-mRNA processing factor 4 homolog B (yeast) (PRPF4B)
ACCESSION   NM_003913
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4062..4766 
                     /label="GFP"  
     misc_feature    4813..5398 
                     /label="PolyA signal"  
     misc_feature    7490..8135 
                     /label="ColE1"  
     misc_feature    5852..6646 
                     /label="Neo"  
     misc_feature    5483..5785 
                     /label="SV40 ori"  
     misc_feature    8150..9010 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4041..4047 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4041 
                     /label="PRPF4B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCGCCGCG GAGACCCAGT CGCTACGGGA GCAGCCAGAG ATGGAAGATG 
     1081 CTAATTCTGA AAAGAGTATA AATGAAGAAA ATGGAGAAGT ATCAGAAGAC CAGTCTCAAA 
     1141 ATAAGCACAG TCGTCACAAA AAAAAGAAGC ATAAACACAG AAGTAAACAT AAGAAACATA 
     1201 AACATTCCTC AGAAGAAGAC AAGGATAAAA AACATAAACA TAAGCATAAA CATAAGAAAC 
     1261 ACAAAAGAAA AGAGGTTATT GATGCTTCTG ATAAAGAGGG TATGTCTCCA GCAAAAAGAA 
     1321 CTAAACTTGA TGATTTAGCT TTGCTAGAAG ACTTGGAAAA ACAGAGAGCC TTGATTAAGG 
     1381 CCGAACTTGA TAATGAGTTA ATGGAAGGAA AGGTCCAGTC TGGTATGGGG CTCATTTTGC 
     1441 AAGGTTATGA GTCTGGCTCT GAAGAAGAGG GGGAAATTCA TGAAAAGGCA AGAAATGGAA 
     1501 ATAGGTCTAG TACTAGATCT TCAAGTACAA AGGGGAAACT TGAACTTGTG GACAATAAAA 
     1561 TTACTACAAA GAAACGAAGT AAAAGCAGAT CCAAAGAACG GACTAGACAT AGGTCTGATA 
     1621 AAAAGAAAAG TAAGGGGGGT ATTGAAATCG TTAAAGAGAA AACAACTAGG AGCAAGTCAA 
     1681 AGGAGAGGAA AAAATCTAAA AGCCCATCCA AAAGAAGTAA GTCTCAAGAT CAAGCAAGGA 
     1741 AATCAAAATC CCCTACCCTT AGAAGGCGAT CTCAAGAGAA AATTGGTAAG GCCAGATCTC 
     1801 CTACTGATGA TAAGGTTAAA ATTGAAGATA AAAGTAAATC AAAAGATAGG AAAAAATCCC 
     1861 CAATTATAAA TGAAAGTAGA AGTCGCGATC GAGGTAAAAA ATCCAGATCC CCAGTTGATT 
     1921 TAAGAGGTAA ATCCAAAGAC AGAAGGTCAC GGTCCAAAGA GAGAAAATCA AAACGGTCTG 
     1981 AAACTGATAA AGAAAAGAAG CCAATTAAAT CTCCCTCTAA AGATGCTTCA TCTGGGAAAG 
     2041 AAAATAGGTC ACCCAGCAGA AGACCTGGTC GTAGTCCTAA AAGAAGAAGT TTGTCTCCAA 
     2101 AACCACGTGA TAAATCAAGA AGAAGCAGGT CTCCACTTTT GAATGATAGA AGATCTAAGC 
     2161 AGAGCAAATC CCCCTCGCGG ACACTGTCTC CTGGGAGAAG AGCCAAGAGC CGATCCTTAG 
     2221 AAAGAAAACG ACGAGAACCA GAGAGGAGAC GACTTTCTTC TCCAAGAACA CGACCTCGAG 
     2281 ATGATATCCT CAGTAGACGT GAAAGATCAA AAGATGCCAG CCCCATCAAT AGATGGTCTC 
     2341 CAACCCGAAG AAGAAGTAGA TCTCCCATTA GAAGGAGGTC TCGTTCCCCA CTCAGACGTA 
     2401 GCAGGTCTCC AAGAAGAAGA AGCAGATCTA CTCGGAGAAG GGACAGAGGT CGGAGGAGCA 
     2461 GATCACGCTT GCGAAGGCGG TCTCGATCAC GCGGTGGTCG TAGACGAAGG AGCAGAAGCA 
     2521 AAGTAAAGGA AGATAAATTT AAAGGAAGTC TTTCTGAAGG AATGAAAGTT GAGCAGGAAT 
     2581 CTTCGTCTGA TGATAACCTT GAAGACTTTG ATGTAGAGGA AGAAGATGAA GAAGCCCTAA 
     2641 TAGAACAGAG AAGAATCCAA AGGCAGGCAA TTGTTCAGAA ATATAAATAC CTTGCTGAAG 
     2701 ATAGCAACAT GTCTGTGCCA TCTGAACCAA GCAGCCCCCA GAGCAGTACG AGAACACGAT 
     2761 CACCATCTCC AGATGACATT CTGGAGCGAG TAGCTGCTGA TGTTAAAGAG TATGAACGGG 
     2821 AAAATGTTGA TACATTTGAG GCCTCAGTGA AAGCCAAGCA TAATCTAATG ACAGTTGAAC 
     2881 AGAATAATGG TTCATCTCAG AAGAAGTTGT TGGCACCTGA TATGTTTACA GAATCTGATG 
     2941 ATATGTTTGC TGCGTATTTT GATAGTGCTC GTCTTCGGGC CGCTGGCATT GGAAAAGATT 
     3001 TCAAAGAGAA TCCCAACCTC AGAGATAACT GGACCGATGC AGAAGGCTAT TATCGTGTGA 
     3061 ACATAGGTGA AGTCCTAGAT AAACGTTACA ATGTGTATGG CTACACTGGG CAAGGTGTAT 
     3121 TCAGTAATGT TGTACGAGCC AGAGATAATG CAAGAGCCAA CCAAGAAGTG GCTGTAAAGA 
     3181 TCATCAGAAA CAATGAGCTC ATGCAAAAGA CTGGTTTAAA AGAATTAGAG TTCTTGAAAA 
     3241 AACTTAATGA TGCTGATCCT GATGACAAAT TTCATTGTCT GAGACTCTTA AGGCACTTCT 
     3301 ATCACAAGCA GCATCTTTGT CTGGTATTCG AGCCTCTCAG CATGAACTTA CGAGAGGTGT 
     3361 TAAAAAAATA TGGTAAAGAT GTTGGTCTTC ATATTAAAGC TGTAAGATCC TATAGTCAGC 
     3421 AGTTGTTCCT GGCATTGAAA CTCCTTAAAA GATGCAATAT CCTACATGCA GATATCAAGC 
     3481 CAGACAATAT CCTGGTTAAT GAATCCAAAA CTATTTTAAA GCTTTGCGAT TTTGGGTCGG 
     3541 CTTCACATGT TGCGGATAAT GACATAACAC CTTATCTTTT CAGTAGATTT TATCGTGCTC 
     3601 CTGAAATCAT TATAGGTAAA AGCTATGACT ATGGTATAGA TATGTGGTCT GTAGGTTGCA 
     3661 CCTTATACGA ACTCTATACT GGAAAAATTT TATTCCCTGG CAAAACCAAT AACCATATGC 
     3721 TGAAGCTTGC AATGGATCTC AAAGGAAAGA TGCCAAATAA GATGATTCGA AAAGGTGTGT 
     3781 TCAAAGATCA GCATTTTGAT CAAAATCTCA ACTTCATGTA CATAGAAGTT GATAAAGTAA 
     3841 CAGAGAGGGA GAAAGTTACT GTTATGAGCA CCATTAATCC AACTAAGGAC CTGTTGGCTG 
     3901 ACTTGATTGG GTGCCAGAGA CTTCCTGAAG ACCAACGTAA GAAAGTACAC CAGCTAAAGG 
     3961 ACTTGTTGGA CCAGATTCTG ATGTTGGACC CAGCTAAACG AATTAGCATC AACCAGGCCC 
     4021 TACAGCACGC CTTCATCCAG GAAAAAATTA CGCGTACGCG GCCGCTCGAG ATGGAGAGCG 
     4081 ACGAGAGCGG CCTGCCCGCC ATGGAGATCG AGTGCCGCAT CACCGGCACC CTGAACGGCG 
     4141 TGGAGTTCGA GCTGGTGGGC GGCGGAGAGG GCACCCCCGA GCAGGGCCGC ATGACCAACA 
     4201 AGATGAAGAG CACCAAAGGC GCCCTGACCT TCAGCCCCTA CCTGCTGAGC CACGTGATGG 
     4261 GCTACGGCTT CTACCACTTC GGCACCTACC CCAGCGGCTA CGAGAACCCC TTCCTGCACG 
     4321 CCATCAACAA CGGCGGCTAC ACCAACACCC GCATCGAGAA GTACGAGGAC GGCGGCGTGC 
     4381 TGCACGTGAG CTTCAGCTAC CGCTACGAGG CCGGCCGCGT GATCGGCGAC TTCAAGGTGA 
     4441 TGGGCACCGG CTTCCCCGAG GACAGCGTGA TCTTCACCGA CAAGATCATC CGCAGCAACG 
     4501 CCACCGTGGA GCACCTGCAC CCCATGGGCG ATAACGATCT GGATGGCAGC TTCACCCGCA 
     4561 CCTTCAGCCT GCGCGACGGC GGCTACTACA GCTCCGTGGT GGACAGCCAC ATGCACTTCA 
     4621 AGAGCGCCAT CCACCCCAGC ATCCTGCAGA ACGGGGGCCC CATGTTCGCC TTCCGCCGCG 
     4681 TGGAGGAGGA TCACAGCAAC ACCGAGCTGG GCATCGTGGA GTACCAGCAC GCCTTCAAGA 
     4741 CCCCGGATGC AGATGCCGGT GAAGAAAGAG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4801 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4861 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4921 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4981 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     5041 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     5101 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5161 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5221 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5281 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5341 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5401 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCTTGGCTG 
     5461 TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 
     5521 CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 
     5581 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT 
     5641 CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA 
     5701 ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG 
     5761 TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC 
     5821 ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA 
     5881 TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA 
     5941 CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT 
     6001 CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG 
     6061 CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA 
     6121 GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC 
     6181 CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT 
     6241 GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT 
     6301 CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG 
     6361 CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG 
     6421 ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC 
     6481 ATCGACTGTG GCCGGCTGGG TGTGGCCGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT 
     6541 GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC 
     6601 GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG 
     6661 GGACTCTGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG 
     6721 ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT 
     6781 GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA 
     6841 TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT 
     6901 TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT 
     6961 GTATACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT 
     7021 GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG 
     7081 CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT 
     7141 TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG 
     7201 GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG 
     7261 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT 
     7321 CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA 
     7381 AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA 
     7441 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC 
     7501 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT 
     7561 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA 
     7621 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG 
     7681 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT 
     7741 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA 
     7801 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT 
     7861 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC 
     7921 AAACCACCGC TGGTAGCGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG 
     7981 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT 
     8041 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA 
     8101 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT 
     8161 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG 
     8221 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA 
     8281 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC 
     8341 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT 
     8401 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG 
     8461 TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA 
     8521 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG 
     8581 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA 
     8641 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG 
     8701 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT 
     8761 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA 
     8821 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA 
     8881 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG 
     8941 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC 
     9001 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT 
     9061 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC 
     9121 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG 
     9181 CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC 
     9241 CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA 
     9301 ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC 
     9361 TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT 
     9421 TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA 
     9481 ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT 
     9541 TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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