LOCUS       RG205493                8699 bp    DNA     circular  SYN 
DEFINITION  LRRN2 (tGFP-tagged) - Human leucine rich repeat neuronal 2 (LRRN2), transcript variant 2
ACCESSION   NM_201630
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3180..3884 
                     /label="GFP"  
     misc_feature    3931..4516 
                     /label="PolyA signal"  
     misc_feature    6608..7253 
                     /label="ColE1"  
     misc_feature    4970..5764 
                     /label="Neo"  
     misc_feature    4601..4903 
                     /label="SV40 ori"  
     misc_feature    7268..8128 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3159..3165 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3159 
                     /label="LRRN2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGCTTCTC GTGGCCCCAC TCTTGCTAGC TTGGGTGGCT GGTGCCACTG 
     1081 CCGCTGTGCC CGTGGTACCC TGGCATGTTC CCTGCCCCCC TCAGTGTGCC TGCCAGATCC 
     1141 GGCCCTGGTA TACGCCCCGC TCGTCCTACC GCGAGGCTAC CACTGTGGAC TGCAATGACC 
     1201 TATTCCTGAC GGCAGTCCCC CCGGCACTCC CCGCAGGCAC ACAGACCCTG CTCCTGCAGA 
     1261 GCAACAGCAT TGTCCGTGTG GACCAGAGTG AGCTGGGCTA CCTGGCCAAT CTCACAGAGC 
     1321 TGGACCTGTC CCAGAACAGC TTTTCGGATG CCCGAGACTG TGATTTCCAT GCCCTGCCCC 
     1381 AGCTGCTGAG CCTGCACCTA GAGGAGAACC AGCTGACCCG GCTGGAGGAC CACAGCTTTG 
     1441 CAGGGCTGGC CAGCCTACAG GAACTCTATC TCAACCACAA CCAGCTCTAC CGCATCGCCC 
     1501 CCAGGGCCTT TTCTGGCCTC AGCAACTTGC TGCGGCTGCA CCTCAACTCC AACCTCCTGA 
     1561 GGGCCATTGA CAGCCGCTGG TTTGAAATGC TGCCCAACTT GGAGATACTC ATGATTGGCG 
     1621 GCAACAAGGT AGATGCCATC CTGGACATGA ACTTCCGGCC CCTGGCCAAC CTGCGTAGCC 
     1681 TGGTGCTAGC AGGCATGAAC CTGCGGGAGA TCTCCGACTA TGCCCTGGAG GGGCTGCAAA 
     1741 GCCTGGAGAG CCTCTCCTTC TATGACAACC AGCTGGCCCG GGTGCCCAGG CGGGCACTGG 
     1801 AACAGGTGCC CGGGCTCAAG TTCCTAGACC TCAACAAGAA CCCGCTCCAG CGGGTAGGGC 
     1861 CGGGGGACTT TGCCAACATG CTGCACCTTA AGGAGCTGGG ACTGAACAAC ATGGAGGAGC 
     1921 TGGTCTCCAT CGACAAGTTT GCCCTGGTGA ACCTCCCCGA GCTGACCAAG CTGGACATCA 
     1981 CCAATAACCC ACGGCTGTCC TTCATCCACC CCCGCGCCTT CCACCACCTG CCCCAGATGG 
     2041 AGACCCTCAT GCTCAACAAC AACGCTCTCA GTGCCTTGCA CCAGCAGACG GCGGAGTCCC 
     2101 TGCCCAACCT GCAGGAGGTA GGTCTCCACG GCAACCCCAT CCGCTGTGAC TGTGTCATCC 
     2161 GCTGGGCCAA TGCCACGGGC ACCCGTGTCC GCTTCATCGA GCCGCAATCC ACCCTGTGTG 
     2221 CGGAGCCTCC GGACCTCCAG CGCCTCCCGG TCCGTGAGGT GCCCTTCCGG GAGATGACGG 
     2281 ACCACTGTTT GCCCCTCATC TCCCCACGAA GCTTCCCCCC AAGCCTCCAG GTAGCCAGTG 
     2341 GAGAGAGCAT GGTGCTGCAT TGCCGGGCAC TGGCCGAACC CGAACCCGAG ATCTACTGGG 
     2401 TCACTCCAGC TGGGCTTCGA CTGACACCTG CCCATGCAGG CAGGAGGTAC CGGGTGTACC 
     2461 CCGAGGGGAC CCTGGAGCTG CGGAGGGTGA CAGCAGAAGA GGCGGGGCTA TACACCTGTG 
     2521 TGGCCCAGAA CCTGGTGGGG GCTGACACTA AGACGGTTAG TGTGGTTGTG GGCCGTGCTC 
     2581 TCCTCCAGCC AGGCAGGGAC GAAGGACAGG GGCTGGAGCT CCGGGTGCAG GAGACCCACC 
     2641 CCTATCACAT CCTGCTATCT TGGGTCACCC CACCCAACAC AGTGTCCACC AACCTCACCT 
     2701 GGTCCAGTGC CTCCTCCCTC CGGGGCCAGG GGGCCACAGC TCTGGCCCGC CTGCCTCGGG 
     2761 GAACCCACAG CTACAACATT ACCCGCCTCC TTCAGGCCAC GGAGTACTGG GCCTGCCTGC 
     2821 AAGTGGCCTT TGCTGATGCC CACACCCAGT TGGCTTGTGT ATGGGCCAGG ACCAAAGAGG 
     2881 CCACTTCTTG CCACAGAGCC TTAGGGGACC GTCCTGGGCT CATTGCCATC CTGGCTCTCG 
     2941 CTGTCCTTCT CCTGGCAGCT GGGCTAGCGG CCCACCTTGG CACAGGCCAA CCCAGGAAGG 
     3001 GTGTGGGTGG GAGGCGGCCT CTCCCTCCAG CCTGGGCTTT CTGGGGCTGG AGTCCCCCTT 
     3061 CTGTCCGGGT TGTGTCTGCT CCCCTCGTCC TGCCCTGGAA TCCAGGGAGG AAGCTGCCCA 
     3121 GATCCTCAGA AGGGGAGACA CTGTTGCCAC CATTGTCTCA AAATTCTACG CGTACGCGGC 
     3181 CGCTCGAGAT GGAGAGCGAC GAGAGCGGCC TGCCCGCCAT GGAGATCGAG TGCCGCATCA 
     3241 CCGGCACCCT GAACGGCGTG GAGTTCGAGC TGGTGGGCGG CGGAGAGGGC ACCCCCGAGC 
     3301 AGGGCCGCAT GACCAACAAG ATGAAGAGCA CCAAAGGCGC CCTGACCTTC AGCCCCTACC 
     3361 TGCTGAGCCA CGTGATGGGC TACGGCTTCT ACCACTTCGG CACCTACCCC AGCGGCTACG 
     3421 AGAACCCCTT CCTGCACGCC ATCAACAACG GCGGCTACAC CAACACCCGC ATCGAGAAGT 
     3481 ACGAGGACGG CGGCGTGCTG CACGTGAGCT TCAGCTACCG CTACGAGGCC GGCCGCGTGA 
     3541 TCGGCGACTT CAAGGTGATG GGCACCGGCT TCCCCGAGGA CAGCGTGATC TTCACCGACA 
     3601 AGATCATCCG CAGCAACGCC ACCGTGGAGC ACCTGCACCC CATGGGCGAT AACGATCTGG 
     3661 ATGGCAGCTT CACCCGCACC TTCAGCCTGC GCGACGGCGG CTACTACAGC TCCGTGGTGG 
     3721 ACAGCCACAT GCACTTCAAG AGCGCCATCC ACCCCAGCAT CCTGCAGAAC GGGGGCCCCA 
     3781 TGTTCGCCTT CCGCCGCGTG GAGGAGGATC ACAGCAACAC CGAGCTGGGC ATCGTGGAGT 
     3841 ACCAGCACGC CTTCAAGACC CCGGATGCAG ATGCCGGTGA AGAAAGAGTT TAAACGGCCG 
     3901 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     3961 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4021 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4081 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4141 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4201 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4261 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4321 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4381 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4441 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4501 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     4561 ACGCGGCCAG CTTGGCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC 
     4621 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG 
     4681 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC 
     4741 ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT 
     4801 CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT 
     4861 GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC 
     4921 CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG ATCGTTTCGC 
     4981 ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC 
     5041 GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA 
     5101 GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG 
     5161 CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG 
     5221 CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG 
     5281 GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG 
     5341 CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC 
     5401 ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA 
     5461 GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG CATGCCCGAC 
     5521 GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT 
     5581 GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCCGACCG CTATCAGGAC 
     5641 ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC 
     5701 CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT 
     5761 GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG ACGCCCAACC 
     5821 TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG 
     5881 TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG 
     5941 CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA 
     6001 ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA 
     6061 ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG TAATCATGGT 
     6121 CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG 
     6181 GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT 
     6241 TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG 
     6301 GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG 
     6361 ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA 
     6421 TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC 
     6481 AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC 
     6541 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT 
     6601 AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC 
     6661 CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT 
     6721 CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG 
     6781 AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC 
     6841 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA 
     6901 GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA 
     6961 GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA 
     7021 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTTT TTTTGTTTGC AAGCAGCAGA 
     7081 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG 
     7141 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT 
     7201 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT 
     7261 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC 
     7321 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG 
     7381 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG 
     7441 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT 
     7501 TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG 
     7561 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT 
     7621 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA 
     7681 TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG 
     7741 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT 
     7801 CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA 
     7861 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA 
     7921 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT 
     7981 TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT 
     8041 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA 
     8101 AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT 
     8161 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA 
     8221 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA 
     8281 GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA 
     8341 GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT 
     8401 TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC 
     8461 ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT 
     8521 AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC 
     8581 AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC 
     8641 CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//