LOCUS       RG204949                9101 bp    DNA     circular  SYN 
DEFINITION  FTSJ3 (tGFP-tagged) - Human FtsJ homolog 3 (E. coli) (FTSJ3)
ACCESSION   NM_017647
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3582..4286 
                     /label="GFP"  
     misc_feature    4333..4918 
                     /label="PolyA signal"  
     misc_feature    7010..7655 
                     /label="ColE1"  
     misc_feature    5372..6166 
                     /label="Neo"  
     misc_feature    5003..5305 
                     /label="SV40 ori"  
     misc_feature    7670..8530 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3561..3567 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3561 
                     /label="FTSJ3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCAAGAAG GGCAAAGTTG GCAAGAGCCG ACGAGACAAG TTTTATCACT 
     1081 TGGCGAAGGA GACGGGTTAC CGTTCCCGAT CTGCTTTCAA GCTGATCCAG CTCAATCGCC 
     1141 GCTTTCAGTT CCTGCAGAAA GCCCGAGCCT TGCTGGACCT GTGTGCTGCG CCAGGGGGAT 
     1201 GGCTGCAGGT AGCTGCCAAG TTTATGCCTG TATCCAGCCT TATTGTGGGA GTGGACCTGG 
     1261 TTCCAATCAA GCCTCTCCCC AATGTGGTGA CTCTCCAGGA GGACATCACA ACAGAACGTT 
     1321 GTAGGCAGGC CCTGAGGAAG GAGCTGAAGA CCTGGAAGGT TGATGTTGTG CTCAATGATG 
     1381 GGGCCCCCAA CGTTGGGGCT AGCTGGGTCC ATGATGCTTA CTCACAAGCC CATTTGACAC 
     1441 TGATGGCTCT ACGTTTGGCT TGTGACTTTT TGGCCCGTGG TGGCAGCTTC ATCACAAAGG 
     1501 TTTTCCGTTC TCGTGACTAT CAGCCTCTGC TATGGATCTT TCAGCAGCTG TTCCGCCGTG 
     1561 TCCAGGCCAC CAAGCCCCAA GCCTCTCGCC ATGAATCTGC AGAGATCTTT GTAGTCTGCC 
     1621 AAGGATTCCT GGCCCCTGAC AAGGTTGACA GTAAATTCTT TGACCCCAAA TTTGCCTTTA 
     1681 AGGAGGTTGA AGTTCAGGCT AAGACCGTTA CTGAATTGGT TACTAAGAAG AAGCCAAAGG 
     1741 CTGAAGGCTA TGCTGAGGGT GACCTCACTC TCTATCACCG TACCTCAGTC ACTGACTTCC 
     1801 TCCGAGCTGC CAACCCTGTT GACTTCCTCT CCAAGGCCAG CGAAATCATG GTAGATGATG 
     1861 AAGAGTTGGC ACAGCATCCA GCTACCACTG AGGACATACG GGTGTGCTGT CAGGACATCA 
     1921 GAGTGTTGGG GCGCAAGGAG CTCAGGTCGC TACTAAACTG GAGAACAAAA CTTCGGCGAT 
     1981 ATGTGGCCAA GAAGCTGAAA GAACAAGCAA AGGCACTGGA CATCAGCCTC AGCTCTGGAG 
     2041 AGGAAGATGA AGGTGATGAG GAGGACTCAA CAGCTGGAAC CACAAAGCAG CCCTCTAAGG 
     2101 AGGAGGAGGA AGAGGAGGAG GAGGAACAAC TGAACCAGAC CTTGGCAGAA ATGAAGGCCC 
     2161 AGGAGGTGGC GGAATTGAAG AGGAAGAAAA AGAAGCTGTT GCGTGAGCAG AGAAAGCAGC 
     2221 GGGAGCGTGT GGAGCTGAAG ATGGATCTGC CTGGGGTTTC CATTGCAGAC GAGGGGGAGA 
     2281 CTGGCATGTT CTCCTTGTGC ACCATCCGGG GTCACCAGTT ATTAGAGGAA GTAACACAAG 
     2341 GGGATATGAG TGCAGCAGAC ACATTTCTGT CCGATCTGCC AAGGGATGAT ATCTATGTGT 
     2401 CAGATGTTGA GGACGACGGT GATGACACAT CTCTGGATAG TGACCTGGAT CCAGAGGAGC 
     2461 TGGCAGGAGT CAGGGGACAT CAGGGTCTAA GGGACCAAAA GCGTATGCGA CTTACTGAAG 
     2521 TGCAAGATGA TAAAGAGGAG GAGGAGGAGG AGAATCCACT GCTGGTACCA CTGGAGGAAA 
     2581 AGGCAGTACT GCAGGAAGAA CAAGCCAACC TGTGGTTCTC AAAGGGCAGC TTTGCTGGGA 
     2641 TCGAGGACGA TGCCGATGAG GCCCTGGAGA TCAGTCAGGC CCAGCTGTTA TTTGAGAACC 
     2701 GGCGGAAGGG ACGGCAGCAG CAGCAGAAGC AGCAGCTGCC ACAGACACCC CCTTCCTGTT 
     2761 TGAAGACTGA GATAATGTCT CCCCTGTACC AAGATGAAGC CCCTAAGGGA ACAGAGGCTT 
     2821 CTTCGGGGAC AGAAGCTGCC ACTGGCCTTG AAGGGGAAGA AAAGGATGGC ATCTCAGACA 
     2881 GTGATAGCAG TACTAGCAGT GAGGAAGAAG AGAGCTGGGA ACCCCTCCGT GGTAAGAAGC 
     2941 GAAGCCGTGG GCCTAAGTCA GATGATGACG GGTTTGAGAT AGTGCCTATT GAGGACCCAG 
     3001 CGAAACATCG GATACTGGAC CCCGAAGGCC TTGCTCTAGG TGCTGTTATT GCCTCTTCCA 
     3061 AAAAGGCCAA GAGAGACCTC ATAGATAACT CCTTCAACCG GTACACATTT AATGAGGATG 
     3121 AGGGGGAGCT TCCGGAGTGG TTTGTGCAAG AGGAAAAGCA GCACCGGATA CGACAGTTGC 
     3181 CTGTTGGTAA GAAGGAGGTG GAGCATTACC GGAAACGCTG GCGGGAAATC AATGCACGTC 
     3241 CCATCAAGAA GGTGGCTGAG GCTAAGGCTA GAAAGAAAAG GAGGATGCTG AAGAGGCTGG 
     3301 AGCAGACCAG GAAGAAGGCA GAAGCCGTGG TGAACACAGT GGACATCTCA GAACGAGAGA 
     3361 AAGTGGCACA GCTGCGAAGT CTCTACAAGA AGGCTGGGCT TGGCAAGGAG AAACGCCATG 
     3421 TCACCTACGT TGTAGCCAAA AAAGGTGTGG GCCGCAAAGT GCGCCGGCCA GCTGGAGTCA 
     3481 GAGGTCATTT CAAGGTGGTG GACTCAAGGA TGAAGAAGGA CCAAAGAGCA CAGCAACGTA 
     3541 AGGAACAAAA GAAAAAACAC AAACGGAAGA CGCGTACGCG GCCGCTCGAG ATGGAGAGCG 
     3601 ACGAGAGCGG CCTGCCCGCC ATGGAGATCG AGTGCCGCAT CACCGGCACC CTGAACGGCG 
     3661 TGGAGTTCGA GCTGGTGGGC GGCGGAGAGG GCACCCCCGA GCAGGGCCGC ATGACCAACA 
     3721 AGATGAAGAG CACCAAAGGC GCCCTGACCT TCAGCCCCTA CCTGCTGAGC CACGTGATGG 
     3781 GCTACGGCTT CTACCACTTC GGCACCTACC CCAGCGGCTA CGAGAACCCC TTCCTGCACG 
     3841 CCATCAACAA CGGCGGCTAC ACCAACACCC GCATCGAGAA GTACGAGGAC GGCGGCGTGC 
     3901 TGCACGTGAG CTTCAGCTAC CGCTACGAGG CCGGCCGCGT GATCGGCGAC TTCAAGGTGA 
     3961 TGGGCACCGG CTTCCCCGAG GACAGCGTGA TCTTCACCGA CAAGATCATC CGCAGCAACG 
     4021 CCACCGTGGA GCACCTGCAC CCCATGGGCG ATAACGATCT GGATGGCAGC TTCACCCGCA 
     4081 CCTTCAGCCT GCGCGACGGC GGCTACTACA GCTCCGTGGT GGACAGCCAC ATGCACTTCA 
     4141 AGAGCGCCAT CCACCCCAGC ATCCTGCAGA ACGGGGGCCC CATGTTCGCC TTCCGCCGCG 
     4201 TGGAGGAGGA TCACAGCAAC ACCGAGCTGG GCATCGTGGA GTACCAGCAC GCCTTCAAGA 
     4261 CCCCGGATGC AGATGCCGGT GAAGAAAGAG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4321 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4381 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4441 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4501 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4561 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4621 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4681 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4741 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4801 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4861 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     4921 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCTTGGCTG 
     4981 TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 
     5041 CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 
     5101 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT 
     5161 CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA 
     5221 ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG 
     5281 TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC 
     5341 ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA 
     5401 TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA 
     5461 CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT 
     5521 CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG 
     5581 CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA 
     5641 GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC 
     5701 CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT 
     5761 GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT 
     5821 CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG 
     5881 CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG 
     5941 ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC 
     6001 ATCGACTGTG GCCGGCTGGG TGTGGCCGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT 
     6061 GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC 
     6121 GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG 
     6181 GGACTCTGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG 
     6241 ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT 
     6301 GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA 
     6361 TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT 
     6421 TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT 
     6481 GTATACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT 
     6541 GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG 
     6601 CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT 
     6661 TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG 
     6721 GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG 
     6781 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT 
     6841 CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA 
     6901 AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA 
     6961 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC 
     7021 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT 
     7081 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA 
     7141 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG 
     7201 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT 
     7261 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA 
     7321 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT 
     7381 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC 
     7441 AAACCACCGC TGGTAGCGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG 
     7501 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT 
     7561 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA 
     7621 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT 
     7681 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG 
     7741 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA 
     7801 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC 
     7861 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT 
     7921 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG 
     7981 TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA 
     8041 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG 
     8101 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA 
     8161 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG 
     8221 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT 
     8281 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA 
     8341 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA 
     8401 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG 
     8461 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC 
     8521 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT 
     8581 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC 
     8641 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG 
     8701 CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC 
     8761 CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA 
     8821 ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC 
     8881 TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT 
     8941 TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA 
     9001 ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT 
     9061 TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//