LOCUS       RG202136                9464 bp    DNA     circular  SYN 
DEFINITION  AARS (tGFP-tagged) - Human alanyl-tRNA synthetase (AARS)
ACCESSION   NM_001605
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3945..4649 
                     /label="GFP"  
     misc_feature    4696..5281 
                     /label="PolyA signal"  
     misc_feature    7373..8018 
                     /label="ColE1"  
     misc_feature    5735..6529 
                     /label="Neo"  
     misc_feature    5366..5668 
                     /label="SV40 ori"  
     misc_feature    8033..8893 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3924..3930 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3924 
                     /label="AARS1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACTCTACT CTAACAGCAA GTGAAATCCG GCAGCGATTT ATAGATTTCT 
     1081 TCAAGAGGAA CGAGCATACG TATGTTCACT CGTCTGCCAC CATCCCATTG GATGACCCCA 
     1141 CTTTGCTCTT TGCCAATGCA GGCATGAACC AGTTTAAACC CATTTTCCTG AACACAATTG 
     1201 ACCCATCTCA CCCCATGGCA AAGCTGAGCA GAGCTGCCAA TACCCAGAAG TGCATCCGGG 
     1261 CTGGGGGCAA ACATAATGAC CTGGACGATG TGGGCAAGGA TGTCTATCAT CACACCTTCT 
     1321 TCGAGATGCT GGGCTCTTGG TCTTTTGGAG ATTACTTTAA GGAATTGGCA TGTAAGATGG 
     1381 CTCTGGAACT CCTCACCCAA GAGTTTGGCA TTCCCATTGA AAGACTTTAT GTTACTTACT 
     1441 TTGGCGGGGA TGAAGCAGCT GGCTTAGAAG CAGATCTGGA ATGCAAACAG ATCTGGCAAA 
     1501 ATTTGGGGCT GGATGACACC AAAATCCTCC CAGGCAACAT GAAGGATAAC TTCTGGGAGA 
     1561 TGGGTGACAC GGGCCCCTGT GGTCCTTGCA GTGAGATCCA CTACGACCGG ATTGGTGGTC 
     1621 GGGACGCCGC ACATCTTGTC AACCAGGACG ACCCTAATGT GCTGGAGATC TGGAACCTTG 
     1681 TGTTCATCCA GTATAACAGG GAAGCTGATG GCATTCTGAA ACCTCTTCCC AAGAAAAGCA 
     1741 TTGACACAGG GATGGGCCTG GAACGACTGG TATCTGTGCT GCAGAATAAG ATGTCCAACT 
     1801 ATGACACTGA CCTTTTTGTC CCTTACTTTG AAGCCATTCA GAAGGGCACA GGTGCCCGAC 
     1861 CATACACTGG GAAAGTTGGT GCTGAGGATG CCGATGGGAT TGACATGGCC TACCGGGTGC 
     1921 TGGCTGACCA TGCTCGGACC ATCACTGTGG CACTGGCTGA TGGTGGCCGG CCTGACAACA 
     1981 CAGGGCGTGG ATATGTGTTG AGACGGATTC TCCGCCGAGC TGTCCGATAC GCCCATGAAA 
     2041 AGCTCAATGC CAGCAGGGGC TTCTTTGCTA CGTTAGTGGA TGTTGTCGTC CAGTCCCTGG 
     2101 GAGATGCATT TCCTGAGCTG AAGAAGGACC CAGACATGGT GAAGGACATC ATTAATGAAG 
     2161 AAGAGGTGCA GTTTCTCAAG ACTCTCAGCA GAGGGCGTCG CATCCTGGAC AGGAAAATTC 
     2221 AGAGCCTGGG AGACAGCAAG ACCATTCCCG GAGACACTGC TTGGCTCCTC TATGACACCT 
     2281 ATGGGTTTCC AGTGGATCTG ACTGGACTGA TTGCTGAAGA GAAGGGCCTG GTGGTAGACA 
     2341 TGGATGGCTT TGAAGAGGAG AGGAAACTGG CCCAGCTGAA ATCACAGGGC AAGGGAGCTG 
     2401 GTGGGGAAGA CCTCATTATG CTGGACATTT ACGCTATCGA AGAGCTCCGG GCACGGGGTC 
     2461 TGGAGGTCAC AGATGATTCC CCAAAGTACA ATTACCATTT GGACTCCAGT GGTAGCTATG 
     2521 TATTTGAGAA CACAGTGGCT ACGGTGATGG CTCTGCGCAG GGAGAAGATG TTCGTGGAAG 
     2581 AGGTGTCCAC AGGCCAGGAG TGTGGAGTGG TGCTGGACAA GACCTGTTTC TATGCTGAGC 
     2641 AAGGAGGCCA GATCTATGAC GAAGGCTACC TGGTGAAGGT GGATGACAGC AGTGAAGATA 
     2701 AAACAGAGTT TACAGTGAAG AATGCTCAGG TCCGAGGAGG GTATGTGCTA CACATTGGAA 
     2761 CCATCTACGG TGACCTGAAA GTGGGGGATC AGGTCTGGCT GTTTATTGAT GAGCCCCGAC 
     2821 GAAGACCCAT CATGAGCAAC CACACAGCTA CGCACATTCT GAACTTCGCC CTGCGCTCAG 
     2881 TGCTTGGGGA AGCTGACCAG AAAGGCTCAT TGGTTGCTCC TGACCGCCTC AGATTTGACT 
     2941 TTACTGCCAA GGGAGCCATG TCCACCCAAC AGATCAAGAA GGCTGAAGAG ATTGCTAATG 
     3001 AGATGATTGA GGCAGCCAAG GCCGTCTATA CCCAGGATTG CCCCCTGGCA GCAGCGAAAG 
     3061 CCATCCAGGG CCTACGGGCT GTGTTTGATG AGACCTATCC TGACCCTGTG CGAGTCGTCT 
     3121 CCATTGGGGT CCCGGTGTCC GAGTTGCTGG ATGACCCCTC TGGGCCTGCT GGCTCCCTGA 
     3181 CTTCTGTTGA GTTCTGTGGG GGAACGCACC TGCGGAACTC GAGTCATGCA GGAGCTTTTG 
     3241 TGATCGTGAC GGAAGAAGCC ATTGCCAAGG GTATCCGGAG GATTGTGGCT GTCACAGGTG 
     3301 CCGAGGCCCA GAAGGCCCTC AGGAAAGCAG AGAGCTTGAA GAAATGTCTC TCTGTCATGG 
     3361 AAGCCAAAGT GAAGGCTCAG ACTGCTCCAA ACAAGGATGT GCAGAGGGAG ATCGCTGACC 
     3421 TTGGAGAGGC CCTGGCCACT GCAGTCATCC CCCAGTGGCA GAAGGATGAA TTGCGGGAGA 
     3481 CTCTCAAATC CCTAAAGAAG GTCATGGATG ACTTGGACCG AGCCAGCAAA GCCGATGTCC 
     3541 AGAAACGAGT GTTAGAGAAG ACGAAGCAGT TCATCGACAG CAACCCCAAC CAGCCTCTTG 
     3601 TCATCCTGGA GATGGAGAGC GGCGCCTCAG CCAAGGCCCT GAATGAAGCC TTGAAGCTCT 
     3661 TCAAGATGCA CTCCCCTCAG ACTTCTGCCA TGCTCTTCAC GGTGGACAAT GAGGCTGGCA 
     3721 AGATCACGTG CCTGTGTCAA GTCCCCCAGA ATGCAGCCAA TCGGGGCTTA AAAGCCAGCG 
     3781 AGTGGGTGCA GCAGGTGTCA GGCTTGATGG ACGGTAAAGG TGGTGGCAAG GATGTGTCTG 
     3841 CACAGGCCAC AGGCAAGAAC GTTGGCTGCC TGCAGGAGGC GCTGCAGCTG GCCACTTCCT 
     3901 TCGCCCAGCT GCGCCTCGGG GATGTAAAGA ACACGCGTAC GCGGCCGCTC GAGATGGAGA 
     3961 GCGACGAGAG CGGCCTGCCC GCCATGGAGA TCGAGTGCCG CATCACCGGC ACCCTGAACG 
     4021 GCGTGGAGTT CGAGCTGGTG GGCGGCGGAG AGGGCACCCC CGAGCAGGGC CGCATGACCA 
     4081 ACAAGATGAA GAGCACCAAA GGCGCCCTGA CCTTCAGCCC CTACCTGCTG AGCCACGTGA 
     4141 TGGGCTACGG CTTCTACCAC TTCGGCACCT ACCCCAGCGG CTACGAGAAC CCCTTCCTGC 
     4201 ACGCCATCAA CAACGGCGGC TACACCAACA CCCGCATCGA GAAGTACGAG GACGGCGGCG 
     4261 TGCTGCACGT GAGCTTCAGC TACCGCTACG AGGCCGGCCG CGTGATCGGC GACTTCAAGG 
     4321 TGATGGGCAC CGGCTTCCCC GAGGACAGCG TGATCTTCAC CGACAAGATC ATCCGCAGCA 
     4381 ACGCCACCGT GGAGCACCTG CACCCCATGG GCGATAACGA TCTGGATGGC AGCTTCACCC 
     4441 GCACCTTCAG CCTGCGCGAC GGCGGCTACT ACAGCTCCGT GGTGGACAGC CACATGCACT 
     4501 TCAAGAGCGC CATCCACCCC AGCATCCTGC AGAACGGGGG CCCCATGTTC GCCTTCCGCC 
     4561 GCGTGGAGGA GGATCACAGC AACACCGAGC TGGGCATCGT GGAGTACCAG CACGCCTTCA 
     4621 AGACCCCGGA TGCAGATGCC GGTGAAGAAA GAGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4681 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4741 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4801 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4861 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4921 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4981 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5041 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5101 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5161 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5221 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5281 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCTTGG 
     5341 CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 
     5401 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA 
     5461 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA 
     5521 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA 
     5581 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG 
     5641 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA 
     5701 TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT 
     5761 GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA 
     5821 CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG 
     5881 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG 
     5941 CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT 
     6001 GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT 
     6061 CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG 
     6121 CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT 
     6181 ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC 
     6241 GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC 
     6301 GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA 
     6361 TTCATCGACT GTGGCCGGCT GGGTGTGGCC GACCGCTATC AGGACATAGC GTTGGCTACC 
     6421 CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT 
     6481 ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA 
     6541 GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT 
     6601 TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG 
     6661 GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT 
     6721 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG 
     6781 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG 
     6841 TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG 
     6901 TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA 
     6961 AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG 
     7021 CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA 
     7081 GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG 
     7141 TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG 
     7201 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC 
     7261 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA 
     7321 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT 
     7381 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC 
     7441 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC 
     7501 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC 
     7561 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT 
     7621 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG 
     7681 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA 
     7741 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA 
     7801 AACAAACCAC CGCTGGTAGC GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA 
     7861 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA 
     7921 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT 
     7981 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA 
     8041 GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA 
     8101 TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC 
     8161 CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA 
     8221 ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC 
     8281 AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA 
     8341 ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT 
     8401 TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG 
     8461 CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC 
     8521 TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT 
     8581 CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT 
     8641 GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC 
     8701 TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT 
     8761 CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA 
     8821 GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA 
     8881 CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG 
     8941 GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG 
     9001 TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG 
     9061 CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG 
     9121 CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC 
     9181 TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA 
     9241 AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC 
     9301 CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC 
     9361 TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT 
     9421 GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//