LOCUS       RG200764                9038 bp    DNA     circular  SYN 
DEFINITION  ELAC2 (tGFP-tagged) - Human elaC homolog 2 (E. coli) (ELAC2), transcript variant 1
ACCESSION   NM_018127
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3519..4223 
                     /label="GFP"  
     misc_feature    4270..4855 
                     /label="PolyA signal"  
     misc_feature    6947..7592 
                     /label="ColE1"  
     misc_feature    5309..6103 
                     /label="Neo"  
     misc_feature    4940..5242 
                     /label="SV40 ori"  
     misc_feature    7607..8467 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3498..3504 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3498 
                     /label="ELAC2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGGGCGCTT TGCTCGCTGC TGCGGTCCGC GGCCGGACGC ACCATGTCGC 
     1081 AGGGACGCAC CATATCGCAG GCACCCGCCC GCCGCGAGCG GCCGCGCAAG GACCCGCTGC 
     1141 GGCACCTGCG CACGCGAGAG AAGCGCGGAC CGTCGGGGTG CTCCGGCGGC CCAAACACCG 
     1201 TGTACCTGCA GGTGGTGGCA GCGGGTAGCC GGGACTCGGG CGCCGCGCTC TACGTCTTCT 
     1261 CCGAGTTCAA CCGGTATCTC TTCAACTGTG GAGAAGGCGT TCAGAGACTC ATGCAGGAGC 
     1321 ACAAGTTAAA GGTTGCTCGC CTGGACAACA TATTCCTGAC ACGAATGCAC TGGTCTAATG 
     1381 TTGGGGGCTT AAGTGGAATG ATTCTTACTT TAAAGGAAAC CGGGCTTCCA AAGTGTGTAC 
     1441 TTTCTGGACC TCCACAACTG GAAAAATACC TCGAAGCAAT CAAAATATTT TCTGGTCCAT 
     1501 TGAAAGGAAT AGAACTGGCT GTGCGGCCCC ACTCTGCCCC AGAATACGAG GATGAAACCA 
     1561 TGACAGTTTA CCAGATCCCC ATACACAGTG AACAGAGGAG GGGAAAGCAC CAACCATGGC 
     1621 AGAGTCCAGA AAGGCCTCTC AGCAGGCTCA GTCCAGAGCG ATCTTCAGAC TCCGAGTTGA 
     1681 ATGAAAATGA GCCACACCTT CCACATGGTG TTAGCCAGAG AAGAGGGGTC AGGGACTCTT 
     1741 CCCTGGTCGT AGCTTTCATC TGTAAGCTTC ACTTAAAGAG AGGAAACTTC TTGGTGCTCA 
     1801 AAGCAAAGGA GATGGGCCTC CCAGTTGGGA CAGCTGCCAT CGCTCCCATC ATTGCTGCTG 
     1861 TCAAGGACGG GAAAAGCATC ACTCATGAAG GAAGAGAGAT TTTGGCTGAA GAGCTGTGTA 
     1921 CTCCTCCAGA TCCTGGTGCT GCTTTTGTGG TGGTAGAATG TCCAGATGAA AGCTTCATTC 
     1981 AACCCATCTG TGAGAATGCC ACCTTTCAGA GGTACCAAGG AAAGGCAGAT GCCCCCGTGG 
     2041 CCTTGGTGGT TCACATGGCC CCAGCATCTG TGCTTGTGGA CAGCAGGTAC CAGCAGTGGA 
     2101 TGGAGAGGTT TGGGCCTGAC ACCCAGCACT TGGTCCTGAA TGAGAACTGT GCCTCAGTTC 
     2161 ACAACCTTCG CAGCCACAAG ATTCAAACCC AGCTCAACCT CATCCACCCG GACATCTTCC 
     2221 CCCTGCTCAC CAGTTTCCGC TGTAAGAAGG AGGGCCCCAC CCTCAGTGTG CCCATGGTTC 
     2281 AGGGTGAATG CCTCCTCAAG TACCAGCTCC GTCCCAGGAG GGAGTGGCAG AGGGATGCCA 
     2341 TTATTACTTG CAATCCTGAG GAATTCATAG TTGAGGCGCT GCAGCTTCCC AACTTCCAGC 
     2401 AGAGCGTGCA GGAGTACAGG AGGAGTGCGC AGGACGGCCC AGCCCCAGCA GAGAAAAGAA 
     2461 GTCAGTACCC AGAAATCATC TTCCTTGGAA CAGGGTCTGC CATCCCGATG AAGATTCGAA 
     2521 ATGTCAGTGC CACACTTGTC AACATAAGCC CCGACACGTC TCTGCTACTG GACTGTGGTG 
     2581 AGGGCACGTT TGGGCAGCTG TGCCGTCATT ACGGAGACCA GGTGGACAGG GTCCTGGGCA 
     2641 CCCTGGCTGC TGTGTTTGTG TCCCACCTGC ACGCAGATCA CCACACGGGC TTGCCAAGTA 
     2701 TCTTGCTGCA GAGAGAACGC GCCTTGGCAT CTTTGGGAAA GCCGCTTCAC CCTTTGCTGG 
     2761 TGGTTGCCCC CAACCAGCTC AAAGCCTGGC TCCAGCAGTA CCACAACCAG TGCCAGGAGG 
     2821 TCCTGCACCA CATCAGTATG ATTCCTGCCA AATGCCTTCA GGAAGGGGCT GAGATCTCCA 
     2881 GTCCTGCAGT GGAAAGATTG ATCAGTTCGC TGTTGCGAAC ATGTGATTTG GAAGAGTTTC 
     2941 AGACCTGTCT GGTGCGGCAC TGCAAGCATG CGTTTGGCTG TGCGCTGGTG CACACCTCTG 
     3001 GCTGGAAAGT GGTCTATTCC GGGGACACCA TGCCCTGCGA GGCTCTGGTC CGGATGGGGA 
     3061 AAGATGCCAC CCTCCTGATA CATGAAGCCA CCCTGGAAGA TGGTTTGGAA GAGGAAGCAG 
     3121 TGGAAAAGAC ACACAGCACA ACGTCCCAAG CCATCAGCGT GGGGATGCGG ATGAACGCGG 
     3181 AGTTCATTAT GCTGAACCAC TTCAGCCAGC GCTATGCCAA GGTCCCCCTC TTCAGCCCCA 
     3241 ACTTCAGCGA GAAAGTGGGA GTTGCCTTTG ACCACATGAA GGTCTGCTTT GGAGACTTTC 
     3301 CAACAATGCC CAAGCTGATT CCCCCACTGA AAGCCCTGTT TGCTGGCGAC ATCGAGGAGA 
     3361 TGGAGGAGCG CAGGGAGAAG CGGGAGCTGC GGCAGGTGCG GGCGGCCCTC CTGTCCAGGG 
     3421 AGCTGGCAGG CGGCCTGGAG GATGGGGAGC CTCAGCAGAA GCGGGCCCAC ACAGAGGAGC 
     3481 CACAGGCCAA GAAGGTCAGA GCCCAGACGC GTACGCGGCC GCTCGAGATG GAGAGCGACG 
     3541 AGAGCGGCCT GCCCGCCATG GAGATCGAGT GCCGCATCAC CGGCACCCTG AACGGCGTGG 
     3601 AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA CCCCCGAGCA GGGCCGCATG ACCAACAAGA 
     3661 TGAAGAGCAC CAAAGGCGCC CTGACCTTCA GCCCCTACCT GCTGAGCCAC GTGATGGGCT 
     3721 ACGGCTTCTA CCACTTCGGC ACCTACCCCA GCGGCTACGA GAACCCCTTC CTGCACGCCA 
     3781 TCAACAACGG CGGCTACACC AACACCCGCA TCGAGAAGTA CGAGGACGGC GGCGTGCTGC 
     3841 ACGTGAGCTT CAGCTACCGC TACGAGGCCG GCCGCGTGAT CGGCGACTTC AAGGTGATGG 
     3901 GCACCGGCTT CCCCGAGGAC AGCGTGATCT TCACCGACAA GATCATCCGC AGCAACGCCA 
     3961 CCGTGGAGCA CCTGCACCCC ATGGGCGATA ACGATCTGGA TGGCAGCTTC ACCCGCACCT 
     4021 TCAGCCTGCG CGACGGCGGC TACTACAGCT CCGTGGTGGA CAGCCACATG CACTTCAAGA 
     4081 GCGCCATCCA CCCCAGCATC CTGCAGAACG GGGGCCCCAT GTTCGCCTTC CGCCGCGTGG 
     4141 AGGAGGATCA CAGCAACACC GAGCTGGGCA TCGTGGAGTA CCAGCACGCC TTCAAGACCC 
     4201 CGGATGCAGA TGCCGGTGAA GAAAGAGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4261 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4321 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4381 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4441 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4501 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4561 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4621 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4681 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4741 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4801 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4861 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC TTGGCTGTGG 
     4921 AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 
     4981 AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 
     5041 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG 
     5101 CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT 
     5161 TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA 
     5221 GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT 
     5281 TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG 
     5341 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG 
     5401 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT 
     5461 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA 
     5521 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG 
     5581 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT 
     5641 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT 
     5701 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG 
     5761 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA 
     5821 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC 
     5881 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC 
     5941 GACTGTGGCC GGCTGGGTGT GGCCGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT 
     6001 ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC 
     6061 GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA 
     6121 CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT 
     6181 CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA 
     6241 TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG 
     6301 CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT 
     6361 TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA 
     6421 TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA 
     6481 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT 
     6541 GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC 
     6601 AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG 
     6661 GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC 
     6721 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG 
     6781 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA 
     6841 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC 
     6901 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC 
     6961 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG 
     7021 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT 
     7081 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC 
     7141 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC 
     7201 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG 
     7261 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG 
     7321 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA 
     7381 CCACCGCTGG TAGCGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT 
     7441 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC 
     7501 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT 
     7561 AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC 
     7621 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG 
     7681 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG 
     7741 CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC 
     7801 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA 
     7861 TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG 
     7921 TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT 
     7981 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA 
     8041 GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG 
     8101 TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA 
     8161 CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT 
     8221 GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA 
     8281 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT 
     8341 CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT 
     8401 CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA 
     8461 AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT 
     8521 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC 
     8581 GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG 
     8641 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT 
     8701 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC 
     8761 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG 
     8821 ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA 
     8881 CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC 
     8941 CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA 
     9001 AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//