LOCUS       RC240171                9456 bp    DNA     circular  SYN 
DEFINITION  IQGAP2 (myc-DDK-tagged) - Human IQ motif containing GTPase activating protein 2 (IQGAP2), transcript variant 2
ACCESSION   NM_001285460
VERSION     NM_001285460.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5613..5704 
                     /label="Myc-DDK"  
     misc_feature    5734..6319 
                     /label="PolyA signal"  
     misc_feature    5791..5810 
                     /label="Restriction Enzyme"  
     misc_feature    6595..7267 
                     /label="ColE1"  
     misc_feature    7697..8491 
                     /label="Kan/Neo"  
     misc_feature    8526..8828 
                     /label="SV40 ori"  
     misc_feature    8914..9391 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5595..5601 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5595 
                     /label="IQGAP2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAAGTTTGC TTAGTTGAAG AATTGCCACC AACCACTGAA TTGGAAGAAG 
     1081 GGCTCCGGAA TGGAGTTTAC CTTGCAAAGT TAGCCAAGTT CTTTGCCCCG AAAATGGTAT 
     1141 CAGAGAAAAA GATCTATGAT GTGGAACAAA CACGTTATAA GAAGTCTGGC CTTCATTTTC 
     1201 GACACACAGA TAATACCGTC CAGTGGTTAA GAGCGATGGA GTCTATTGGT CTACCCAAGA 
     1261 TATTTTATCC AGAAACAACA GATGTCTATG ATCGGAAAAA CATACCAAGA ATGATATATT 
     1321 GCATTCACGC ACTGAGTTTG TATCTGTTCA AACTAGGAAT AGCACCCCAG ATCCAGGATT 
     1381 TGTTGGGCAA AGTAGACTTC ACAGAGGAGG AAATCAGTAA TATGAGAAAA GAACTTGAGA 
     1441 AATATGGAAT ACAGATGCCA TCTTTCAGCA AAATAGGTGG TATTCTGGCC AATGAACTGT 
     1501 CCGTGGATGA AGCTGCATTA CATGCTGCAG TTATAGCCAT TAATGAAGCA GTTGAAAAAG 
     1561 GAATAGCAGA GCAAACCGTT GTAACACTAA GAAACCCAAA TGCGGTTTTA ACTTTAGTGG 
     1621 ATGACAACCT TGCACCAGAA TATCAGAAAG AACTCTGGGA TGCCAAAAAG AAAAAAGAGG 
     1681 AAAATGCAAG ACTGAAGAAT AGCTGTATTT CAGAAGAAGA AAGAGATGCT TATGAAGAAC 
     1741 TGCTGACACA AGCAGAAATC CAAGGCAATA TTAATAAAGT CAACAGGCAG GCTGCAGTGG 
     1801 ACCATATCAA TGCTGTCATT CCGGAAGGTG ACCCCGAGAA TACGCTGCTT GCACTGAAGA 
     1861 AACCAGAGGC CCAGCTGCCT GCTGTTTATC CCTTTGCTGC TGCCATGTAT CAGAACGAAC 
     1921 TTTTCAACCT CCAGAAACAG AACACCATGA ACTACTTGGC CCACGAGGAG CTTTTGATTG 
     1981 CTGTGGAAAT GTTGTCTGCT GTTGCTTTAC TAAACCAGGC CTTGGAAAGC AACGATCTTG 
     2041 TGTCTGTGCA GAATCAACTC AGAAGCCCCG CAATAGGCTT AAACAATCTG GACAAGGCAT 
     2101 ATGTGGAACG TTATGCAAAC ACACTACTCT CTGTTAAACT AGAAGTTTTA TCCCAAGGGC 
     2161 AAGATAACTT AAGCTGGAAT GAAATTCAGA ATTGTATTGA TATGGTTAAT GCTCAAATTC 
     2221 AAGAAGAAAA TGACCGAGTT GTAGCTGTAG GGTACATCAA TGAAGCTATT GATGAAGGGA 
     2281 ATCCTTTGAG GACTTTAGAA ACTTTGCTCC TACCTACTGC GAATATTAGT GATGTGGACC 
     2341 CAGCCCATGC CCAGCACTAC CAGGATGTTT TATACCATGC TAAATCACAG AAACTCGGAG 
     2401 ACTCTGAGAG TGTTTCCAAA GTGCTTTGGC TGGATGAGAT ACAGCAAGCC GTCGATGATG 
     2461 CCAACGTGGA CGAGGACAGA GCAAAACAAT GGGTTACTCT GGTGGTTGAT GTTAATCAGT 
     2521 GTTTGGAAGG AAAAAAATCA AGTGATATTT TGTCTGTATT GAAGTCTTCC ACTTCTAATG 
     2581 CAAATGACAT AATCCCGGAG TGTGCTGACA AATACTATGA TGCCCTTGTG AAGGCAAAAG 
     2641 AGCTCAAATC TGAAAGAGTG TCTAGTGACG GTTCATGGCT CAAACTCAAC CTGCACAAAA 
     2701 AATATGACTA CTATTACAAC ACTGATTCAA AAGAGAGTTC CTGGGTCACA CCTGAATCAT 
     2761 GCTTGTATAA AGAATCATGG CTCACAGGAA AAGAAATCGA GGACATTATT GAGGAAGTCA 
     2821 CAGTAGGTTA CATTCGTGAG AATATATGGT CTGCTTCAGA AGAGTTGCTT CTTCGCTTTC 
     2881 AAGCCACAAG CTCAGGACCC ATCCTTAGGG AAGAGTTTGA AGCTAGAAAA TCATTTTTGC 
     2941 ATGAACAAGA AGAGAATGTG GTCAAAATAC AGGCTTTTTG GAAAGGATAT AAACAACGGA 
     3001 AGGAGTATAT GCACAGGCGG CAAACGTTCA TTGATAATAC TGATTCTATT GTGAAGATTC 
     3061 AGTCCTGGTT CCGAATGGCA ACTGCAAGAA AGAGCTATCT TTCAAGACTA CAGTATTTCA 
     3121 GAGATCATAA TAATGAAATT GTGAAAATAC AGTCACTGTT GAGAGCGAAC AAAGCTAGAG 
     3181 ATGACTACAA AACATTGGTT GGCTCTGAAA ACCCACCATT AACAGTAATT CGCAAATTTG 
     3241 TATACCTGCT GGACCAAAGT GATTTGGATT TCCAGGAGGA ACTAGAGGTT GCACGATTAA 
     3301 GGGAAGAAGT AGTGACCAAG ATCAGGGCCA ATCAACAGCT GGAAAAAGAC CTGAACCTGA 
     3361 TGGACATCAA GATTGGACTG CTGGTGAAGA ACAGGATCAC ACTAGAGGAT GTAATTTCAC 
     3421 ACAGTAAAAA GCTGAACAAG AAAAAAGGAG GAGAAATGGA AATACTGAAT AACACCGACA 
     3481 ACCAAGGAAT AAAAAGTTTG AGTAAGGAGA GGAGAAAAAC ACTAGAAACA TATCAGCAGC 
     3541 TGTTTTACCT TTTACAGACC AACCCTTTAT ACTTGGCTAA GCTGATTTTC CAGATGCCAC 
     3601 AGAACAAGTC CACTAAATTT ATGGATACTG TTATTTTCAC ACTATATAAT TATGCCTCTA 
     3661 ATCAGCGAGA AGAATATCTA CTTCTCAAGC TTTTTAAAAC TGCTCTGGAG GAAGAAATAA 
     3721 AATCAAAAGT GGACCAGGTA CAGGACATAG TTACTGGTAA CCCTACAGTC ATCAAGATGG 
     3781 TCGTCAGCTT CAATAGAGGT GCCCGGGGAC AGAACACCCT GCGCCAACTC CTGGCTCCAG 
     3841 TGGTAAAAGA GATCATCGAC GACAAGTCGC TGATTATCAA CACAAACCCT GTAGAGGTGT 
     3901 ACAAGGCTTG GGTGAACCAA CTAGAAACAC AGACTGGAGA GGCCAGCAAG TTGCCTTATG 
     3961 ATGTGACCAC AGAACAAGCT CTAACATACC CAGAAGTGAA AAATAAACTG GAGGCTTCCA 
     4021 TTGAGAACCT GAGAAGGGTC ACCGACAAAG TCCTGAATTC TATCATTTCT TCCCTTGATC 
     4081 TACTGCCTTA TGGATTGAGG TATATAGCCA AAGTACTGAA GAATTCGATC CATGAGAAAT 
     4141 TCCCCGATGC AACAGAAGAT GAGCTATTAA AGATTGTTGG AAACCTCCTG TACTATCGGT 
     4201 ACATGAATCC AGCCATTGTA GCTCCAGATG GCTTTGATAT CATCGACATG ACAGCTGGAG 
     4261 GTCAGATAAA TTCTGACCAA AGGAGAAACT TAGGATCAGT GGCCAAGGTT CTTCAGCACG 
     4321 CAGCCTCCAA CAAGCTGTTT GAAGGAGAAA ATGAGCATCT CTCATCTATG AACAATTATT 
     4381 TATCAGAGAC GTATCAGGAA TTCAGGAAAT ATTTCAAAGA AGCATGTAAT GTCCCTGAGC 
     4441 CAGAAGAGAA GTTTAATATG GACAAATACA CAGACCTGGT GACAGTCAGC AAACCAGTCA 
     4501 TTTATATTTC AATTGAAGAA ATCATCAGCA CACACTCACT CCTGTTGGAA CACCAGGATG 
     4561 CAATTGCCCC TGAGAAAAAT GACTTACTGA GTGAATTGCT GGGGTCGCTG GGAGAGGTGC 
     4621 CAACCGTGGA ATCTTTTCTT GGGGAAGGAG CAGTTGACCC CAATGACCCT AACAAGGCAA 
     4681 ATACACTAAG TCAGCTTTCA AAGACCGAGA TTTCTCTTGT CTTGACAAGC AAATATGACA 
     4741 TAGAGGACGG TGAAGCTATA GATAGCCGAA GCCTCATGAT AAAGACCAAG AAGCTGATAA 
     4801 TTGATGTGAT CCGGAACCAG CCAGGGAACA CATTGACAGA AATCTTAGAG ACACCAGCAA 
     4861 CTGCGCAACA GGAGGTAGAC CATGCCACGG ACATGGTGAG CCGTGCAATG ATAGATTCCA 
     4921 GGACTCCAGA AGAAATGAAG CATAGCCAAT CTATGATTGA AGATGCACAG CTGCCTCTTG 
     4981 AGCAGAAGAA GAGGAAAATC CAGAGGAATC TTCGGACGTT GGAACAGACT GGACACGTGT 
     5041 CATCCGAAAA TAAATACCAA GACATTCTCA ATGAGATTGC CAAGGATATT CGAAATCAAA 
     5101 GAATCTATCG TAAGCTTCGA AAAGCTGAAT TGGCAAAACT TCAGCAGACC CTGAATGCAC 
     5161 TTAACAAGAA GGCAGCATTT TATGAAGAGC AAATCAATTA TTATGACACC TACATAAAGA 
     5221 CTTGTTTAGA CAACTTAAAA AGAAAAAATA CTCGGAGATC AATTAAACTA GATGGAAAAG 
     5281 GAGAACCCAA AGGGGCGAAG AGAGCGAAGC CAGTGAAGTA CACTGCAGCA AAGCTGCATG 
     5341 AGAAAGGTGT CCTGCTAGAT ATAGATGATC TTCAAACAAA CCAGTTTAAG AATGTTACAT 
     5401 TTGATATCAT AGCTACTGAA GATGTAGGCA TTTTCGATGT AAGATCAAAA TTCCTTGGTG 
     5461 TTGAGATGGA AAAGGTGCAA CTCAATATTC AGGATTTACT TCAGATGCAA TATGAAGGAG 
     5521 TAGCTGTAAT GAAAATGTTT GATAAGGTTA AAGTGAATGT AAACCTTCTC ATATACCTGC 
     5581 TGAACAAGAA GTTCTATGGA AAGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     5641 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     5701 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     5761 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     5821 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     5881 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5941 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     6001 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     6061 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     6121 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     6181 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     6241 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     6301 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     6361 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     6421 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     6481 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     6541 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     6601 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     6661 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     6721 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     6781 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     6841 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6901 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6961 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     7021 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     7081 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     7141 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     7201 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     7261 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     7321 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     7381 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     7441 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     7501 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     7561 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     7621 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     7681 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     7741 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     7801 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     7861 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7921 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7981 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     8041 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     8101 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     8161 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     8221 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     8281 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     8341 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     8401 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     8461 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     8521 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     8581 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     8641 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     8701 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8761 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8821 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     8881 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8941 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     9001 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     9061 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     9121 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     9181 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     9241 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     9301 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     9361 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     9421 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//