LOCUS       RC240170                9444 bp    DNA     circular  SYN 
DEFINITION  SLIT2 (myc-DDK-tagged) - Human slit homolog 2 (Drosophila) (SLIT2), transcript variant 3
ACCESSION   NM_001289136
VERSION     NM_001289136.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5601..5692 
                     /label="Myc-DDK"  
     misc_feature    5722..6307 
                     /label="PolyA signal"  
     misc_feature    5779..5798 
                     /label="Restriction Enzyme"  
     misc_feature    6583..7255 
                     /label="ColE1"  
     misc_feature    7685..8479 
                     /label="Kan/Neo"  
     misc_feature    8514..8816 
                     /label="SV40 ori"  
     misc_feature    8902..9379 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5583..5589 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5583 
                     /label="SLIT2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGCGGCGTT GGCTGGCAGA TGCTGTCCCT GTCGCTGGGG TTAGTGCTGG 
     1081 CGATCCTGAA CAAGGTGGCA CCGCAGGCGT GCCCGGCGCA GTGCTCTTGC TCGGGCAGCA 
     1141 CAGTGGACTG TCACGGGCTG GCGCTGCGCA GCGTGCCCAG GAATATCCCC CGCAACACCG 
     1201 AGAGACTGGA TTTAAATGGA AATAACATCA CAAGAATTAC GAAGACAGAT TTTGCTGGTC 
     1261 TTAGACATCT AAGAGTTCTT CAGCTTATGG AGAATAAGAT TAGCACCATT GAAAGAGGAG 
     1321 CATTCCAGGA TCTTAAAGAA CTAGAGAGAC TGCGTTTAAA CAGAAATCAC CTTCAGCTGT 
     1381 TTCCTGAGTT GCTGTTTCTT GGGACTGCGA AGCTATACAG GCTTGATCTC AGTGAAAACC 
     1441 AAATTCAGGC AATCCCAAGG AAAGCTTTCC GTGGGGCAGT TGACATAAAA AATTTGCAAC 
     1501 TGGATTACAA CCAGATCAGC TGTATTGAAG ATGGGGCATT CAGGGCTCTC CGGGACCTGG 
     1561 AAGTGCTCAC TCTCAACAAT AACAACATTA CTAGACTTTC TGTGGCAAGT TTCAACCATA 
     1621 TGCCTAAACT TAGGACTTTT CGACTGCATT CAAACAACCT GTATTGTGAC TGCCACCTGG 
     1681 CCTGGCTCTC CGACTGGCTT CGCCAAAGGC CTCGGGTTGG TCTGTACACT CAGTGTATGG 
     1741 GCCCCTCCCA CCTGAGAGGC CATAATGTAG CCGAGGTTCA AAAACGAGAA TTTGTCTGCA 
     1801 GTGGTCACCA GTCATTTATG GCTCCTTCTT GTAGTGTTTT GCACTGCCCT GCCGCCTGTA 
     1861 CCTGTAGCAA CAATATCGTA GACTGTCGTG GGAAAGGTCT CACTGAGATC CCCACAAATC 
     1921 TTCCAGAGAC CATCACAGAA ATACGTTTGG AACAGAACAC AATCAAAGTC ATCCCTCCTG 
     1981 GAGCTTTCTC ACCATATAAA AAGCTTAGAC GAATTGACCT GAGCAATAAT CAGATCTCTG 
     2041 AACTTGCACC AGATGCTTTC CAAGGACTAC GCTCTCTGAA TTCACTTGTC CTCTATGGAA 
     2101 ATAAAATCAC AGAACTCCCC AAAAGTTTAT TTGAAGGACT GTTTTCCTTA CAGCTCCTAT 
     2161 TATTGAATGC CAACAAGATA AACTGCCTTC GGGTAGATGC TTTTCAGGAT CTCCACAACT 
     2221 TGAACCTTCT CTCCCTATAT GACAACAAGC TTCAGACCAT CGCCAAGGGG ACCTTTTCAC 
     2281 CTCTTCGGGC CATTCAAACT ATGCATTTGG CCCAGAACCC CTTTATTTGT GACTGCCATC 
     2341 TCAAGTGGCT AGCGGATTAT CTCCATACCA ACCCGATTGA GACCAGTGGT GCCCGTTGCA 
     2401 CCAGCCCCCG CCGCCTGGCA AACAAAAGAA TTGGACAGAT CAAAAGCAAG AAATTCCGTT 
     2461 GTTCAGGTAC AGAAGATTAT CGATCAAAAT TAAGTGGAGA CTGCTTTGCG GATCTGGCTT 
     2521 GCCCTGAAAA GTGTCGCTGT GAAGGAACCA CAGTAGATTG CTCTAATCAA AAGCTCAACA 
     2581 AAATCCCGGA GCACATTCCC CAGTACACTG CAGAGTTGCG TCTCAATAAT AATGAATTTA 
     2641 CCGTGTTGGA AGCCACAGGA ATCTTTAAGA AACTTCCTCA ATTACGTAAA ATAAACTTTA 
     2701 GCAACAATAA GATCACAGAT ATTGAGGAGG GAGCATTTGA AGGAGCATCT GGTGTAAATG 
     2761 AAATACTTCT TACGAGTAAT CGTTTGGAAA ATGTGCAGCA TAAGATGTTC AAGGGATTGG 
     2821 AAAGCCTCAA AACTTTGATG TTGAGAAGCA ATCGAATAAC CTGTGTGGGG AATGACAGTT 
     2881 TCATAGGACT CAGTTCTGTG CGTTTGCTTT CTTTGTATGA TAATCAAATT ACTACAGTTG 
     2941 CACCAGGGGC ATTTGATACT CTCCATTCTT TATCTACTCT AAACCTCTTG GCCAATCCTT 
     3001 TTAACTGTAA CTGCTACCTG GCTTGGTTGG GAGAGTGGCT GAGAAAGAAG AGAATTGTCA 
     3061 CGGGAAATCC TAGATGTCAA AAACCATACT TCCTGAAAGA AATACCCATC CAGGATGTGG 
     3121 CCATTCAGGA CTTCACTTGT GATGACGGAA ATGATGACAA TAGTTGCTCC CCACTTTCTC 
     3181 GCTGTCCTAC TGAATGTACT TGCTTGGATA CAGTCGTCCG ATGTAGCAAC AAGGGTTTGA 
     3241 AGGTCTTGCC GAAAGGTATT CCAAGAGATG TCACAGAGTT GTATCTGGAT GGAAACCAAT 
     3301 TTACACTGGT TCCCAAGGAA CTCTCCAACT ACAAACATTT AACACTTATA GACTTAAGTA 
     3361 ACAACAGAAT AAGCACGCTT TCTAATCAGA GCTTCAGCAA CATGACCCAG CTCCTCACCT 
     3421 TAATTCTTAG TTACAACCGT CTGAGATGTA TTCCTCCTCG CACCTTTGAT GGATTAAAGT 
     3481 CTCTTCGATT ACTTTCTCTA CATGGAAATG ACATTTCTGT TGTGCCTGAA GGTGCTTTCA 
     3541 ATGATCTTTC TGCATTATCA CATCTAGCAA TTGGAGCCAA CCCTCTTTAC TGTGATTGTA 
     3601 ACATGCAGTG GTTATCCGAC TGGGTGAAGT CGGAATATAA GGAGCCTGGA ATTGCTCGTT 
     3661 GTGCTGGTCC TGGAGAAATG GCAGATAAAC TTTTACTCAC AACTCCCTCC AAAAAATTTA 
     3721 CCTGTCAAGG TCCTGTGGAT GTCAATATTC TAGCTAAGTG TAACCCCTGC CTATCAAATC 
     3781 CGTGTAAAAA TGATGGCACA TGTAATAGTG ATCCAGTTGA CTTTTACCGA TGCACCTGTC 
     3841 CATATGGTTT CAAGGGGCAG GACTGTGATG TCCCAATTCA TGCCTGCATC AGTAACCCAT 
     3901 GTAAACATGG AGGAACTTGC CACTTAAAGG AAGGAGAAGA AGATGGATTC TGGTGTATTT 
     3961 GTGCTGATGG ATTTGAAGGA GAAAATTGTG AAGTCAACGT TGATGATTGT GAAGATAATG 
     4021 ACTGTGAAAA TAATTCTACA TGTGTCGATG GCATTAATAA CTACACATGC CTTTGCCCAC 
     4081 CTGAGTATAC AGGTGAGTTG TGTGAGGAGA AGCTGGACTT CTGTGCCCAG GACCTGAACC 
     4141 CCTGCCAGCA CGATTCAAAG TGCATCCTAA CTCCAAAGGG ATTCAAATGT GACTGCACAC 
     4201 CAGGGTACGT AGGTGAACAC TGCGACATCG ATTTTGACGA CTGCCAAGAC AACAAGTGTA 
     4261 AAAACGGAGC CCACTGCACA GATGCAGTGA ACGGCTATAC GTGCATATGC CCCGAAGGTT 
     4321 ACAGTGGCTT GTTCTGTGAG TTTTCTCCAC CCATGGTCCT CCCTCGTACC AGCCCCTGTG 
     4381 ATAATTTTGA TTGTCAGAAT GGAGCTCAGT GTATCGTCAG AATAAATGAG CCAATATGTC 
     4441 AGTGTTTGCC TGGCTATCAG GGAGAAAAGT GTGAAAAATT GGTTAGTGTG AATTTTATAA 
     4501 ACAAAGAGTC TTATCTTCAG ATTCCTTCAG CCAAGGTTCG GCCTCAGACG AACATAACAC 
     4561 TTCAGATTGC CACAGATGAA GACAGCGGAA TCCTCCTGTA TAAGGGTGAC AAAGACCATA 
     4621 TCGCGGTAGA ACTCTATCGG GGGCGTGTTC GTGCCAGCTA TGACACCGGC TCTCATCCAG 
     4681 CTTCTGCCAT TTACAGTGTG GAGACAATCA ATGATGGAAA CTTCCACATT GTGGAACTAC 
     4741 TTGCCTTGGA TCAGAGTCTC TCTTTGTCCG TGGATGGTGG GAACCCCAAA ATCATCACTA 
     4801 ACTTGTCAAA GCAGTCCACT CTGAATTTTG ACTCTCCACT CTATGTAGGA GGCATGCCAG 
     4861 GGAAGAGTAA CGTGGCATCT CTGCGCCAGG CCCCTGGGCA GAACGGAACC AGCTTCCACG 
     4921 GCTGCATCCG GAACCTTTAC ATCAACAGTG AGCTGCAGGA CTTCCAGAAG GTGCCGATGC 
     4981 AAACAGGCAT TTTGCCTGGC TGTGAGCCAT GCCACAAGAA GGTGTGTGCC CATGGCACAT 
     5041 GCCAGCCCAG CAGCCAGGCA GGCTTCACCT GCGAGTGCCA GGAAGGATGG ATGGGGCCCC 
     5101 TCTGTGACCA ACGGACCAAT GACCCTTGCC TTGGAAATAA ATGCGTACAT GGCACCTGCT 
     5161 TGCCCATCAA TGCGTTCTCC TACAGCTGTA AGTGCTTGGA GGGCCATGGA GGTGTCCTCT 
     5221 GTGATGAAGA GGAGGATCTG TTTAACCCAT GCCAGGCGAT CAAGTGCAAG CATGGGAAGT 
     5281 GCAGGCTTTC AGGTCTGGGG CAGCCCTACT GTGAATGCAG CAGTGGATAC ACGGGGGACA 
     5341 GCTGTGATCG AGAAATCTCT TGTCGAGGGG AAAGGATAAG AGATTATTAC CAAAAGCAGC 
     5401 AGGGCTATGC TGCTTGCCAA ACAACCAAGA AGGTGTCCCG ATTAGAGTGC AGAGGTGGGT 
     5461 GTGCAGGAGG GCAGTGCTGT GGACCGCTGA GGAGCAAGCG GCGGAAATAC TCTTTCGAAT 
     5521 GCACTGACGG CTCCTCCTTT GTGGACGAGG TTGAGAAAGT GGTGAAGTGC GGCTGTACGA 
     5581 GGTGTGTGTC CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     5641 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     5701 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     5761 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     5821 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5881 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5941 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     6001 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     6061 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     6121 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     6181 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     6241 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     6301 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     6361 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     6421 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     6481 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     6541 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     6601 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     6661 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     6721 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     6781 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6841 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6901 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6961 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     7021 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     7081 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     7141 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     7201 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     7261 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     7321 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     7381 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     7441 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     7501 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     7561 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     7621 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     7681 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     7741 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     7801 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7861 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7921 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7981 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     8041 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     8101 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     8161 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     8221 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     8281 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     8341 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     8401 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     8461 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     8521 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     8581 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     8641 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     8701 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8761 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8821 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8881 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8941 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     9001 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     9061 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     9121 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     9181 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     9241 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     9301 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     9361 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     9421 TAACAAAAAT TTAACGCGAA TTTT
//