LOCUS       RC240152                9240 bp    DNA     circular  SYN 
DEFINITION  KDM6A (myc-DDK-tagged) - Human lysine (K)-specific demethylase 6A (KDM6A), transcript variant 1
ACCESSION   NM_001291415
VERSION     NM_001291415.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5397..5488 
                     /label="Myc-DDK"  
     misc_feature    5518..6103 
                     /label="PolyA signal"  
     misc_feature    5575..5594 
                     /label="Restriction Enzyme"  
     misc_feature    6379..7051 
                     /label="ColE1"  
     misc_feature    7481..8275 
                     /label="Kan/Neo"  
     misc_feature    8310..8612 
                     /label="SV40 ori"  
     misc_feature    8698..9175 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5379..5385 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5379 
                     /label="KDM6A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAATCCTGC GGAGTGTCGC TCGCTACCGC CGCCGCTGCC GCCGCCGCTT 
     1081 TCGGTGATGA GGAAAAGAAA ATGGCGGCGG GAAAAGCGAG CGGCGAGAGC GAGGAGGCGT 
     1141 CCCCCAGCCT GACAGCCGAG GAGAGGGAGG CGCTCGGCGG ACTGGACAGC CGCCTCTTTG 
     1201 GGTTCGTGAG ATTTCATGAA GATGGCGCCA GGACGAAGGC CCTACTGGGC AAGGCTGTTC 
     1261 GCTGCTATGA ATCTCTAATC TTAAAAGCTG AAGGAAAAGT GGAGTCTGAT TTCTTTTGTC 
     1321 AATTAGGTCA CTTCAACCTC TTATTGGAAG ATTATCCAAA AGCATTATCT GCATACCAGA 
     1381 GGTACTACAG TTTACAGTCT GACTACTGGA AGAATGCTGC CTTTTTATAT GGTCTTGGTT 
     1441 TGGTCTACTT CCATTATAAT GCATTTCAGT GGGCAATTAA AGCATTTCAG GAGGTGCTTT 
     1501 ATGTTGATCC CAGCTTTTGT CGAGCCAAGG AAATTCATTT ACGACTTGGG CTTATGTTCA 
     1561 AAGTGAACAC AGACTATGAG TCTAGTTTAA AGCATTTTCA GTTAGCTTTG GTTGACTGTA 
     1621 ATCCCTGCAC TTTGTCCAAT GCTGAAATTC AATTTCACAT TGCCCACTTA TATGAAACCC 
     1681 AGAGGAAATA TCATTCTGCA AAAGAAGCTT ATGAACAACT TTTGCAGACA GAGAATCTTT 
     1741 CTGCACAAGT AAAAGCAACT GTCTTACAAC AGTTAGGTTG GATGCATCAC ACTGTAGATC 
     1801 TCCTGGGAGA TAAAGCCACC AAGGAAAGCT ATGCTATTCA GTATCTCCAA AAGTCCTTGG 
     1861 AAGCAGATCC TAATTCTGGC CAGTCCTGGT ATTTCCTCGG AAGGTGCTAT TCAAGTATTG 
     1921 GGAAAGTTCA GGATGCCTTT ATATCTTACA GGCAGTCTAT TGATAAATCA GAAGCAAGTG 
     1981 CAGATACATG GTGTTCAATA GGTGTGCTAT ATCAGCAGCA AAATCAGCCC ATGGATGCTT 
     2041 TACAGGCCTA TATTTGTGCT GTACAATTGG ACCATGGCCA TGCTGCAGCC TGGATGGACC 
     2101 TAGGCACTCT CTATGAATCC TGCAACCAGC CTCAGGATGC CATTAAATGC TACTTAAATG 
     2161 CAACTAGAAG CAAAAGTTGT AGTAATACCT CTGCACTTGC AGCACGAATT AAGTATTTAC 
     2221 AGGCTCAGTT GTGTAACCTT CCACAAGGTA GTCTACAGAA TAAAACTAAA TTACTTCCTA 
     2281 GTATTGAGGA GGCGTGGAGC CTACCAATTC CCGCAGAGCT TACCTCCAGG CAGGGTGCCA 
     2341 TGAACACAGC ACAGCAGGCA TGTAAACCTC ATCATCCAAA TACTGAACCT GTATTAGGCC 
     2401 TCAGTCAAAC ACCAATTTCA CAGCAATCCT TGCCACTACA CATGATTCCT TCTAGCCAAG 
     2461 TAGATGACCT GTCCAGTCCT GCCAAGAGGA AAAGAACATC TAGTCCAACA AAGAATACTT 
     2521 CTGACAATTG GAGTGGTGGA CATGCTGTGT CACATCCTCC AGTACAGCAA CAAGCTCATT 
     2581 CATGGTGTTT GACACCACAG AAATTACAGC ATTTGGAACA GCTCCGCGCA AATAGAAATA 
     2641 ATTTAAATCC AGCACAGAAA CTGATGCTGG AACAGCTGGA AAGTCAGTTT GTCTTAATGC 
     2701 AACAACACCA AATGAGACCA ACAGGAGTTG CACAGGTACG ATCTACTGGA ATTCCTAATG 
     2761 GGCCAACAGC TGACTCATCA CTGCCTACAA ACTCAGTCTC TGGCCAGCAG CCACAGCTTG 
     2821 CTCTGACCAG AGTGCCTAGC GTCTCTCAGC CTGGAGTCCG TCCTGCCTGC CCTGGGCAGC 
     2881 CTTTGGCCAA TGGACCCTTT TCTGCAGGCC ATGTTCCCTG TAGCACATCA AGAACGCTGG 
     2941 GAAGTACAGA CACTATTTTG ATAGGCAATA ATCATATAAC AGGAAGTGGA AGTAATGGAA 
     3001 ACGTGCCTTA CCTGCAGCGA AACGCACTCA CTCTACCTCA TAACCGCACA AACCTGACCA 
     3061 GCAGCGCAGA GGAGCCGTGG AAAAACCAAC TATCTAACTC CACTCAGGGG CTTCACAAAG 
     3121 GTCAGAGTTC ACATTCGGCA GGTCCTAATG GTGAACGACC TCTCTCTTCC ACTGGGCCTT 
     3181 CCCAGCATCT CCAGGCAGCT GGCTCTGGTA TTCAGAATCA GAACGGACAT CCCACCCTGC 
     3241 CTAGCAATTC AGTAACACAG GGGGCTGCTC TCAATCACCT CTCCTCTCAC ACTGCTACCT 
     3301 CAGGTGGACA ACAAGGCATT ACCTTAACCA AAGAGAGCAA GCCTTCAGGA AACATATTGA 
     3361 CGGTGCCTGA AACAAGCAGG CACACTGGAG AGACACCTAA CAGCACTGCC AGTGTCGAGG 
     3421 GACTTCCTAA TCATGTCCAT CAGATGACGG CAGATGCTGT TTGCAGTCCT AGCCATGGAG 
     3481 ATTCTAAGTC ACCAGGTTTA CTAAGTTCAG ACAATCCTCA GCTCTCTGCC TTGTTGATGG 
     3541 GAAAAGCCAA TAACAATGTG GGTACTGGAA CCTGTGACAA AGTCAATAAC ATCCACCCAG 
     3601 CTGTTCATAC AAAGACTGAT AACTCTGTTG CCTCTTCACC ATCTTCAGCC ATTTCAACAG 
     3661 CAACACCTTC TCCAAAATCC ACTGAGCAGA CAACCACAAA CAGTGTTACC AGCCTTAACA 
     3721 GCCCTCACAG TGGGCTACAC ACAATTAATG GAGAAGGGAT GGAAGAATCT CAGAGCCCCA 
     3781 TGAAAACAGA TCTGCTTCTG GTTAACCACA AACCTAGTCC ACAGATCATA CCATCAATGT 
     3841 CTGTGTCCAT ATACCCCAGC TCAGCAGAAG TTCTGAAGGC ATGCAGGAAT CTAGGTAAAA 
     3901 ATGGCTTATC TAACAGTAGC ATTTTGTTGG ATAAATGTCC ACCTCCAAGA CCACCATCTT 
     3961 CACCATACCC TCCCTTGCCA AAGGACAAGT TGAATCCACC TACACCTAGT ATTTACTTGG 
     4021 AAAATAAACG TGATGCTTTC TTTCCTCCAT TACATCAATT TTGTACAAAT CCGAACAACC 
     4081 CTGTTACAGT AATACGTGGC CTTGCTGGAG CTCTTAAGTT AGACCTGGGA CTTTTCTCTA 
     4141 CTAAAACTTT GGTGGAAGCT AACAATGAAC ATATGGTAGA AGTGAGGACA CAGTTGTTGC 
     4201 AGCCAGCAGA TGAAAACTGG GATCCCACTG GAACAAAGAA AATCTGGCAT TGTGAAAGTA 
     4261 ATAGATCTCA TACTACAATT GCTAAATATG CACAGTACCA GGCCTCCTCA TTCCAGGAAT 
     4321 CATTGAGAGA AGAAAATGAA AAAAGAAGTC ATCATAAAGA CCACTCAGAT AGTGAATCTA 
     4381 CATCGTCAGA TAATTCTGGG AGGAGGAGGA AAGGACCCTT TAAAACCATA AAGTTTGGGA 
     4441 CCAATATTGA CCTATCTGAT GACAAAAAGT GGAAGTTGCA GCTACATGAG CTGACTAAAC 
     4501 TTCCTGCTTT TGTGCGTGTC GTATCAGCAG GAAATCTTCT AAGCCATGTT GGTCATACCA 
     4561 TATTGGGCAT GAACACAGTT CAACTATACA TGAAAGTTCC AGGGAGCAGA ACACCAGGTC 
     4621 ATCAGGAAAA TAACAACTTC TGTTCAGTTA ACATAAATAT TGGCCCAGGT GACTGTGAAT 
     4681 GGTTTGTTGT TCCTGAAGGT TACTGGGGTG TTCTGAATGA CTTCTGTGAA AAAAATAATT 
     4741 TGAATTTCCT AATGGGTTCT TGGTGGCCCA ATCTTGAAGA TCTTTATGAA GCAAATGTTC 
     4801 CAGTGTATAG GTTTATTCAG CGACCTGGAG ATTTGGTCTG GATAAATGCA GGCACTGTTC 
     4861 ATTGGGTTCA GGCTATTGGC TGGTGCAACA ACATTGCTTG GAATGTTGGT CCACTTACAG 
     4921 CCTGCCAGTA TAAATTGGCA GTGGAACGGT ACGAATGGAA CAAATTGCAA AGTGTGAAGT 
     4981 CAATAGTACC CATGGTTCAT CTTTCCTGGA ATATGGCACG AAATATCAAG GTCTCAGATC 
     5041 CAAAGCTTTT TGAAATGATT AAGTATTGTC TTCTAAGAAC TCTGAAGCAA TGTCAGACAT 
     5101 TGAGGGAAGC TCTCATTGCT GCAGGAAAAG AGATTATATG GCATGGGCGG ACAAAAGAAG 
     5161 AACCAGCTCA TTACTGTAGC ATTTGTGAAG TGGAGGTTTT TGATCTGCTT TTTGTCACTA 
     5221 ATGAGAGTAA TTCACGAAAG ACCTACATAG TACATTGCCA AGATTGTGCA CGAAAAACAA 
     5281 GCGGAAACTT GGAAAACTTT GTGGTGCTAG AACAGTACAA AATGGAGGAC CTGATGCAAG 
     5341 TCTATGACCA ATTTACATTA GCTCCTCCAT TACCATCCGC CTCATCTACG CGTACGCGGC 
     5401 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     5461 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     5521 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     5581 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     5641 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     5701 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     5761 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5821 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5881 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5941 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     6001 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     6061 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     6121 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     6181 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     6241 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     6301 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     6361 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     6421 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     6481 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     6541 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     6601 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     6661 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     6721 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6781 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6841 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6901 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6961 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     7021 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     7081 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     7141 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     7201 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     7261 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     7321 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     7381 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     7441 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     7501 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     7561 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     7621 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     7681 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     7741 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7801 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7861 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7921 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7981 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     8041 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     8101 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     8161 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     8221 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     8281 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     8341 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     8401 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     8461 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     8521 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     8581 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     8641 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     8701 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     8761 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8821 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8881 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8941 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     9001 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     9061 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     9121 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     9181 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//