LOCUS       RC240149                9210 bp    DNA     circular  SYN 
DEFINITION  ROBO2 (myc-DDK-tagged) - Human roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant 4
ACCESSION   NM_001290040
VERSION     NM_001290040.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5367..5458 
                     /label="Myc-DDK"  
     misc_feature    5488..6073 
                     /label="PolyA signal"  
     misc_feature    5545..5564 
                     /label="Restriction Enzyme"  
     misc_feature    6349..7021 
                     /label="ColE1"  
     misc_feature    7451..8245 
                     /label="Kan/Neo"  
     misc_feature    8280..8582 
                     /label="SV40 ori"  
     misc_feature    8668..9145 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5349..5355 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5349 
                     /label="ROBO2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTCTGCTG ATGTTTACAC AACTACTGCT CTGTGGATTT TTATATGTTC 
     1081 GGGTTGATGG ATCGCGTCTT CGCCAGGAGG ACTTTCCCCC GCGGATTGTG GAGCATCCTT 
     1141 CCGATGTCAT CGTCTCTAAG GGCGAGCCCA CGACTCTGAA CTGCAAGGCG GAGGGCCGGC 
     1201 CAACGCCCAC CATTGAGTGG TACAAAGATG GGGAGCGAGT GGAGACTGAC AAGGACGATC 
     1261 CCCGGTCCCA CAGGATGCTT CTGCCCAGCG GATCCTTATT CTTCTTGCGC ATCGTGCACG 
     1321 GGCGCAGGAG TAAACCTGAT GAAGGAAGCT ACGTTTGTGT TGCGAGGAAC TATCTTGGTG 
     1381 AAGCAGTGAG TCGAAATGCG TCTCTGGAAG TGGCATTGTT ACGAGATGAC TTCCGACAAA 
     1441 ACCCCACAGA TGTTGTAGTG GCAGCTGGAG AGCCTGCAAT CCTGGAGTGC CAGCCTCCCC 
     1501 GGGGACACCC AGAACCCACC ATCTACTGGA AAAAAGACAA AGTTCGAATT GATGACAAGG 
     1561 AAGAAAGAAT AAGTATCCGT GGTGGAAAAC TGATGATCTC CAATACCAGG AAAAGTGATG 
     1621 CAGGGATGTA TACTTGTGTT GGTACCAATA TGGTGGGAGA AAGGGACAGT GACCCAGCAG 
     1681 AGCTGACTGT CTTTGAACGA CCCACATTTC TCAGGAGGCC AATTAACCAG GTGGTACTGG 
     1741 AGGAAGAAGC TGTAGAATTT CGTTGTCAAG TCCAAGGAGA TCCTCAACCA ACTGTGAGGT 
     1801 GGAAAAAGGA TGATGCAGAC TTGCCAAGAG GAAGGTATGA CATCAAAGAC GATTACACAC 
     1861 TAAGAATTAA AAAGACCATG AGTACAGATG AAGGCACCTA TATGTGTATT GCTGAGAATC 
     1921 GGGTTGGAAA AATGGAAGCC TCTGCTACAC TCACCGTCCG AGCTCGCCCT GTTGCTCCCC 
     1981 CACAGTTTGT GGTTCGGCCA AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC 
     2041 CCTGTGAAAC TAAAGGAAAC CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA 
     2101 ACCTACTTTT CCCAAACCAA CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG 
     2161 GAGACCTCAC AATCACCAAC ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT 
     2221 TAACTGTGGC AGGAAGCATT TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG 
     2281 ATAGACCTCC ACCTATAATT CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA 
     2341 CAGCGTTACT GAAATGTAAA GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG 
     2401 AGGGATTTAC TTTTCCGGGT AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC 
     2461 AGATTAAGAA TTTACGGATT TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA 
     2521 GTGGAGAGAC TTCCTGGAGT GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA 
     2581 AAAACTATGA TTTAAGTGAC CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA 
     2641 CTAAGAACAG TGTCACCTTG TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG 
     2701 CATATATCAT TGAGGCTTTC AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC 
     2761 ATGTAAAGAC CACCCTCTAT ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA 
     2821 TGGTCAGAGC GATCAACCCC CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC 
     2881 GCACACAAGA TATCAGCCCA CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC 
     2941 TAGGAGATGT CCTTGTCCGT CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG 
     3001 TCACATGGAC GGTTGATCGC CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC 
     3061 AGACTTCAGG TCTGCAGGCG ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG 
     3121 AACGAAGTGC TGTCTTAGTC AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC 
     3181 CATATTTTAA TGAGTTCCAA GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG 
     3241 AAGCCCCAAG TGCCCCACCA CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA 
     3301 CAAGTATTAG TGTTTCCTGG GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG 
     3361 AATACAAGAT CTGGTGTCTA GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG 
     3421 CAGCCATTCG GTCCGTAATA ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG 
     3481 TTGCAGCTAG TACCAGTGCA GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG 
     3541 GGAGACGCAA TGAAGTTGTC ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG 
     3601 ATGTGGTGAA GCAACCAGCC TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA 
     3661 TGGGTTTTAG CATATGGTTG TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG 
     3721 CTGTTACGTT TCAAAGAGGA GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC 
     3781 TCAATGCTGG TGATCCCAGC TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC 
     3841 CAGTAAATAA TAGCAACAGT GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC 
     3901 TGCCACCAGT TCCAGGCCAA GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT 
     3961 ATAGTAGCAT TGACTTCACT ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG 
     4021 CTACCCCATA TGCCACGACA CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG 
     4081 ATCTGCCTGA TCCACAATGG AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG 
     4141 GTTATTCTCT ACCTGATCAG AACAAAGGTA ACAATGGTGG GAAAGGTGGA AAAAAGAAGA 
     4201 AAAATAAAAA CTCTTCTAAA CCACAGAAAA ACAATGGATC CACTTGGGCC AATGTCCCTC 
     4261 TACCTCCCCC CCCAGTCCAG CCCCTTCCTG GCACGGAGCT GGAACACTAT GCAGTGGAAC 
     4321 AACAAGAAAA TGGCTATGAC AGTGATAGCT GGTGCCCACC ATTGCCAGTA CAAACTTACT 
     4381 TACACCAAGG TCTGGAAGAT GAACTGGAAG AAGATGATGA TAGGGTCCCA ACACCTCCTG 
     4441 TTCGAGGCGT GGCTTCTTCT CCTGCTATCT CCTTTGGACA GCAGTCCACT GCAACTCTTA 
     4501 CTCCATCCCC ACGGGAAGAG ATGCAACCCA TGCTGCAGGC TCACCTGGAT GAGTTGACAA 
     4561 GAGCCTATCA GTTTGATATA GCAAAACAAA CATGGCACAT TCAAAGCAAT AATCAACCTC 
     4621 CACAGCCTCC AGTTCCACCG TTAGGTTATG TGTCTGGAGC CTTGATTTCT GATTTGGAAA 
     4681 CGGATGTTGC AGATGATGAT GCCGACGACG AAGAGGAAGC TTTAGAAATC CCCAGGCCCC 
     4741 TGAGAGCACT GGACCAGACT CCTGGATCCA GCATGGACAA TCTAGACAGC TCTGTGACAG 
     4801 GTTCAATGGT AAATGGATGG GGCTCTGCAT CTGATGAGGA TCGTAACTTT TCTAGTCATA 
     4861 GATCTAGTGT AGGTAGCTCC TCAGATGGCT CTATCTTTGC CAGCGGCAGT TTTGCACAAG 
     4921 CACTGGTGGC AGCAGCAGAT AAAGCTGGTT TTAGGCTGGA TGGAACCAGC CTTACAAGAA 
     4981 CAGGAAAAGC CTTTACCTCC TCTCAAAGAC CTCGACCTAC CAGCCCATTT TCTACTGACA 
     5041 GTAACACCAG TGCAGCCCTG AGTCAAAGTC AGAGGCCTCG GCCCACTAAA AAACACAAGG 
     5101 GAGGGCGGAT GGACCAACAA CCAGCATTGC CTCATCGAAG GGAAGGAATG ACAGATGAGG 
     5161 AGGCCTTGGT GCCCTATAGC AAGCCCAGTT TCCCATCTCC AGGTGGCCAC AGCTCATCAG 
     5221 GAACAGCTTC TTCTAAGGGA TCCACTGGAC CTAGGAAAAC CGAGGTGTTG AGAGCAGGCC 
     5281 ACCAGCGCAA TGCCAGCGAC CTTCTTGACA TAGGATATAT GGGCTCCAAC AGTCAAGGAC 
     5341 AGTTTACAGG TGAATTAACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     5401 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5461 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5521 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5581 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5641 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5701 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5761 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5821 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5881 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5941 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     6001 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     6061 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     6121 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     6181 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     6241 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     6301 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     6361 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6421 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6481 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6541 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6601 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6661 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6721 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6781 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6841 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6901 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6961 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     7021 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     7081 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     7141 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     7201 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     7261 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     7321 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     7381 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7441 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7501 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7561 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7621 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7681 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7741 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7801 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7861 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7921 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7981 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     8041 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     8101 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     8161 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     8221 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     8281 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     8341 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     8401 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8461 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8521 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8581 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8641 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8701 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8761 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8821 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8881 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8941 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     9001 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     9061 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     9121 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     9181 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//