LOCUS       RC240147                9204 bp    DNA     circular  SYN 
DEFINITION  ARHGEF12 (myc-DDK-tagged) - Human Rho guanine nucleotide exchange factor (GEF) 12 (ARHGEF12), transcript variant 3
ACCESSION   NM_001301084
VERSION     NM_001301084.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5361..5452 
                     /label="Myc-DDK"  
     misc_feature    5482..6067 
                     /label="PolyA signal"  
     misc_feature    5539..5558 
                     /label="Restriction Enzyme"  
     misc_feature    6343..7015 
                     /label="ColE1"  
     misc_feature    7445..8239 
                     /label="Kan/Neo"  
     misc_feature    8274..8576 
                     /label="SV40 ori"  
     misc_feature    8662..9139 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5343..5349 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5343 
                     /label="ARHGEF12"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGGGCTGGA GTACAGACAG GTGATCGAAT CATCAAGGTG AATGGAACTC 
     1081 TGGTGACTCA TTCAAATCAT CTGGAGGTGG TGAAGCTAAT CAAATCTGGT TCCTATGTAG 
     1141 CTCTCACTGT TCAGGGACGC CCACCTGGGT CGCCCCAGAT TCCACTTGCC GACTCTGAAG 
     1201 TAGAGCCGTC AGTCATTGGA CATATGTCTC CCATCATGAC ATCTCCTCAT TCACCTGGAG 
     1261 CATCTGGGAA TATGGAGAGA ATCACTAGTC CTGTGCTCAT GGGGGAGGAA AACAATGTGG 
     1321 TTCATAACCA GAAAGTAGAA ATTCTGAGAA AAATGTTACA GAAAGAACAG GAACGGCTAC 
     1381 AGTTATTGCA GGAAGATTAC AACCGAACAC CTGCCCAAAG ATTGCTAAAA GAGATCCAAG 
     1441 AGGCCAAGAA ACACATTCCT CAGCTGCAAG AGCAGTTATC CAAAGCCACA GGCTCTGCTC 
     1501 AGGATGGAGC TGTAGTTACA CCCTCCAGAC CTTTAGGGGA CACCCTAACA GTCAGTGAGG 
     1561 CAGAAACAGA TCCTGGAGAT GTACTGGGCA GGACTGACTG TAGCAGTGGA GATGCTTCTC 
     1621 GGCCCAGTAG TGACAATGCA GATAGTCCCA AGAGTGGCCC AAAAGAGAGA ATTTATCTAG 
     1681 AGGAAAACCC AGAGAAAAGT GAAACAATTC AGGACACTGA CACTCAATCA CTTGTCGGAA 
     1741 GTCCCTCAAC CCGTATAGCA CCTCATATTA TTGGAGCAGA AGATGATGAT TTTGGTACTG 
     1801 AACATGAACA GATCAATGGA CAGTGCAGCT GTTTCCAGAG CATTGAATTA CTAAAATCTC 
     1861 GCCCGGCTCA TTTGGCTGTT TTCTTACACC ATGTAGTTTC ACAATTTGAC CCTGCGACTT 
     1921 TGCTCTGTTA TCTCTATTCA GACCTGTATA AACATACCAA TTCCAAAGAA ACTCGTCGCA 
     1981 TCTTCCTTGA GTTTCATCAG TTCTTTCTAG ATCGATCAGC ACACCTGAAA GTTTCTGTTC 
     2041 CTGATGAAAT GTCTGCAGAT CTAGAAAAGA GAAGACCTGA GCTCATTCCT GAGGATCTGC 
     2101 ATCGCCACTA TATCCAAACT ATGCAAGAAA GAGTCCATCC AGAAGTTCAA AGGCACTTAG 
     2161 AAGATTTTCG GCAGAAACGT AGTATGGGAC TGACCTTGGC TGAAAGCGAG CTGACTAAAC 
     2221 TTGATGCAGA GCGAGACAAG GACCGATTGA CTTTGGAGAA GGAGCGGACA TGTGCAGAAC 
     2281 AGATTGTTGC CAAAATTGAA GAAGTATTGA TGACTGCTCA GGCTGTAGAG GAAGATAAGA 
     2341 GCTCCACCAT GCAGTATGTT ATTCTCATGT ATATGAAGCA TTTGGGAGTA AAAGTGAAAG 
     2401 AGCCTCGAAA TTTGGAGCAC AAACGGGGTC GGATTGGATT TCTTCCCAAA ATCAAGCAAA 
     2461 GTATGAAGAA AGATAAAGAA GGGGAAGAAA AAGGGAAGCG AAGAGGATTC CCCAGCATCC 
     2521 TGGGACCCCC ACGGAGACCA AGCCGTCATG ACAACAGTGC AATTGGCAGA GCCATGGAAC 
     2581 TACAGAAGGC GCGCCACCCT AAGCACTTAT CCACACCCTC ATCTGTGAGT CCTGAACCTC 
     2641 AGGACTCTGC CAAGTTGCGC CAGAGTGGGT TAGCAAATGA AGGAACAGAC GCTGGATACC 
     2701 TGCCTGCCAA TTCCATGTCT TCTGTAGCTT CAGGGGCCTC TTTTTCCCAG GAAGGAGGGA 
     2761 AAGAGAATGA TACAGGATCA AAGCAAGTTG GAGAAACATC AGCACCTGGA GACACCTTAG 
     2821 ATGGCACACC TCGTACTCTC AATACTGTCT TTGATTTCCC ACCACCTCCA TTAGACCAAG 
     2881 TGCAGGAGGA GGAATGTGAA GTAGAAAGGG TGACTGAACA TGGGACACCA AAGCCCTTTC 
     2941 GAAAGTTTGA CAGTGTAGCT TTTGGAGAAA GTCAAAGTGA GGATGAACAA TTTGAAAATG 
     3001 ACTTAGAGAC AGATCCACCC AACTGGCAGC AGCTTGTTAG TCGAGAAGTG TTACTGGGAC 
     3061 TAAAACCTTG TGAAATCAAA AGACAGGAAG TGATTAATGA ATTGTTCTAC ACTGAAAGAG 
     3121 CTCATGTTCG AACACTGAAG GTTCTTGATC AAGTGTTCTA TCAGCGAGTA TCCAGAGAAG 
     3181 GAATTCTGTC ACCCTCAGAG CTACGGAAAA TTTTTTCAAA CTTGGAAGAT ATTCTTCAAC 
     3241 TTCATATTGG ATTGAATGAA CAAATGAAGG CTGTTCGAAA GAGAAATGAG ACCTCTGTTA 
     3301 TCGATCAGAT TGGGGAAGAT TTGCTGACAT GGTTCAGCGG ACCAGGAGAG GAGAAATTGA 
     3361 AACATGCTGC TGCTACCTTT TGCAGTAACC AACCTTTCGC CCTGGAAATG ATCAAATCTC 
     3421 GTCAGAAAAA GGATTCTCGA TTTCAGACTT TTGTGCAAGA TGCTGAAAGT AATCCACTGT 
     3481 GTCGTCGTCT TCAACTGAAG GATATTATTC CCACTCAAAT GCAAAGGCTT ACTAAGTACC 
     3541 CACTTCTGTT GGATAATATT GCCAAATACA CAGAATGGCC AACAGAAAGG GAGAAGGTGA 
     3601 AGAAAGCTGC AGATCACTGT CGTCAGATCT TAAATTATGT AAATCAGGCT GTCAAGGAGG 
     3661 CAGAAAACAA GCAGCGCCTA GAAGATTATC AGCGTCGCCT TGATACCTCC AGCCTGAAGT 
     3721 TGTCAGAGTA CCCAAATGTT GAAGAGCTCA GGAATTTGGA TTTAACAAAA AGGAAGATGA 
     3781 TTCATGAAGG GCCATTGGTT TGGAAGGTGA ATAGAGATAA AACTATTGAT TTATACACGT 
     3841 TGCTGCTGGA AGACATTCTT GTATTGTTAC AAAAGCAGGA TGATAGACTG GTTTTAAGGT 
     3901 GTCATAGTAA GATTCTGGCA TCTACAGCTG ATAGCAAACA CACGTTTAGC CCTGTCATTA 
     3961 AGTTGAGTAC AGTGTTGGTT CGACAAGTGG CAACAGATAA CAAAGCTTTA TTCGTCATTT 
     4021 CCATGTCAGA CAATGGCGCT CAGATTTATG AACTGGTGGC ACAGACAGTT TCTGAAAAGA 
     4081 CTGTCTGGCA GGACCTAATC TGTCGGATGG CTGCATCAGT GAAGGAGCAA TCCACAAAGC 
     4141 CAATTCCATT ACCACAGTCA ACACCTGGCG AAGGAGATAA TGATGAAGAA GATCCTTCAA 
     4201 AATTAAAAGA GGAGCAGCAT GGCATTTCAG TCACTGGTTT GCAGAGTCCA GACAGAGATT 
     4261 TGGGATTAGA ATCTACCTTA ATATCGTCAA AACCTCAGTC TCATTCACTG AGTACCTCTG 
     4321 GGAAATCAGA GGTACGTGAT CTGTTTGTGG CTGAGAGACA GTTTGCAAAG GAACAACATA 
     4381 CAGATGGGAC ACTAAAGGAA GTTGGAGAAG ATTATCAAAT CGCAATCCCA GATTCACACC 
     4441 TGCCTGTCTC AGAAGAACGG TGGGCATTGG ATGCACTAAG AAATTTGGGT TTGTTGAAGC 
     4501 AGTTGCTGGT GCAACAGCTA GGTTTGACTG AGAAGAGCGT TCAGGAAGAC TGGCAACATT 
     4561 TCCCAAGATA CAGAACAGCC TCTCAGGGGC CGCAGACAGA CAGTGTCATC CAGAACTCTG 
     4621 AAAATATTAA GGCCTATCAT TCTGGTGAAG GACATATGCC CTTTAGAACT GGAACTGGTG 
     4681 ACATTGCAAC TTGTTACAGT CCACGGACTT CAACTGAATC TTTTGCTCCA CGGGATTCAG 
     4741 TGGGACTGGC ACCCCAGGAT AGCCAGGCAA GTAACATTTT AGTAATGGAC CACATGATTA 
     4801 TGACCCCAGA GATGCCTACC ATGGAGCCAG AAGGGGGTCT TGATGACAGT GGAGAGCACT 
     4861 TTTTTGATGC CCGTGAAGCA CATAGTGATG AGAATCCATC AGAAGGTGAT GGAGCAGTTA 
     4921 ACAAGGAAGA GAAGGATGTT AATTTACGCA TCTCAGGAAA CTATTTGATC CTTGATGGCT 
     4981 ATGACCCAGT GCAGGAGAGT TCCACAGATG AGGAGGTTGC TTCCTCACTT ACCCTGCAGC 
     5041 CCATGACAGG CATCCCTGCT GTGGAATCCA CCCACCAGCA GCAACATTCT CCTCAGAATA 
     5101 CTCACTCCGA TGGGGCAATT TCACCATTCA CCCCCGAATT TCTGGTCCAG CAGCGCTGGG 
     5161 GAGCTATGGA GTATTCCTGT TTTGAGATCC AGAGTCCCTC CTCTTGTGCA GATTCACAGA 
     5221 GCCAGATCAT GGAGTACATT CATAAGATAG AGGCTGACCT TGAACACTTA AAGAAGGTGG 
     5281 AGGAAAGTTA CACCATTCTT TGCCAAAGGC TGGCTGGATC AGCCCTCACA GACAAGCACT 
     5341 CAGATAAAAG TACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     5401 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     5461 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     5521 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     5581 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5641 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5701 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5761 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5821 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5881 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5941 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     6001 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     6061 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     6121 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     6181 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     6241 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     6301 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     6361 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     6421 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     6481 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     6541 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6601 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6661 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6721 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6781 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6841 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6901 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6961 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     7021 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     7081 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     7141 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     7201 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     7261 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     7321 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     7381 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     7441 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     7501 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     7561 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7621 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7681 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7741 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7801 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7861 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7921 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7981 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     8041 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     8101 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     8161 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     8221 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     8281 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     8341 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     8401 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     8461 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8521 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8581 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8641 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8701 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8761 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8821 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8881 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8941 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     9001 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     9061 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     9121 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     9181 TAACAAAAAT TTAACGCGAA TTTT
//