LOCUS       RC240142                9180 bp    DNA     circular  SYN 
DEFINITION  NUP153 (myc-DDK-tagged) - Human nucleoporin 153kDa (NUP153), transcript variant 3
ACCESSION   NM_001278210
VERSION     NM_001278210.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5337..5428 
                     /label="Myc-DDK"  
     misc_feature    5458..6043 
                     /label="PolyA signal"  
     misc_feature    5515..5534 
                     /label="Restriction Enzyme"  
     misc_feature    6319..6991 
                     /label="ColE1"  
     misc_feature    7421..8215 
                     /label="Kan/Neo"  
     misc_feature    8250..8552 
                     /label="SV40 ori"  
     misc_feature    8638..9115 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5319..5325 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5319 
                     /label="NUP153"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCTCGGGA GCCGGAGGAG TCGGAGGGGG CGGTGGCGGC AAGATCCGGA 
     1081 CGCGGCGTTG CCACCAGGGG CCAATTAAGC CTTACCAGCA GGGGCGACAA CAGCATCAGG 
     1141 GCATTCTTAG CAGGGTTACA GAATCTGTTA AGAATATTGT GCCAGGGTGG CTACAAAGAT 
     1201 ACTTCAACAA GAATGAAGAT GTATGCAGCT GTTCAACAGA CACAAGCGAG GTTCCACGCT 
     1261 GGCCAGAAAA TAAAGAGGAC CATCTGGTAT ATGCCGATGA GGAGAGCTCT AATATTACTG 
     1321 ATGGGAGAAT CACACCTGAG CCAGCAGTCA GTAATACAGA AGAACCTTCA ACAACTAGTA 
     1381 CTGCTTCAAA TTATCCAGAT GTGTTAACAA GGCCTTCTCT TCATCGGAGC CATCTGAATT 
     1441 TTTCCATGTT GGAATCCCCT GCATTACACT GTCAGCCATC TACATCCTCG GCATTCCCAA 
     1501 TTGGCAGTTC GGGATTTTCC CTTGTAAAGG AAATTAAAGA TTCTACCTCT CAGCATGATG 
     1561 ATGATAACAT CTCAACTACC AGTGGTTTTT CTTCAAGAGC TTCTGATAAA GATATAACTG 
     1621 TTTCAAAGAA CACTTCATTG CCACCTCTGT GGTCCCCAGA AGCTGAACGT TCTCACTCAC 
     1681 TCTCACAGCA CACTGCCACC AGCTCAAAAA AACCAGCATT CAACTTGTCT GCCTTTGGAA 
     1741 CACTTTCCCC TTCACTTGGG AATTCTTCAA TCCTTAAAAC CAGTCAGCTT GGAGATTCTC 
     1801 CTTTTTATCC TGGAAAAACA ACATACGGTG GGGCAGCAGC TGCTGTAAGA CAGTCTAAAC 
     1861 TACGAAATAC ACCTTATCAG GCACCAGTTA GAAGACAAAT GAAAGCTAAG CAACTCAGTG 
     1921 CACAATCTTA CGGTGTGACC AGTTCAACAG CTCGGCGAAT ATTGCAGTCT TTAGAGAAGA 
     1981 TGTCAAGCCC TTTAGCGGAT GCAAAAAGAA TTCCATCCAT TGTTTCTTCT CCTCTGAATT 
     2041 CTCCTCTTGA TAGGAGTGGG ATAGATATCA CAGATTTTCA GGCCAAAAGA GAAAAGGTGG 
     2101 ATTCTCAATA TCCTCCTGTT CAGAGACTTA TGACCCCAAA GCCAGTTTCC ATAGCAACAA 
     2161 ATCGAAGTGT TTATTTTAAA CCATCTCTGA CTCCTTCTGG TGAATTCAGG AAGACTAATC 
     2221 AAAGAATAGA TAACAAGTGC AGTACTGGAT ATGAAAAAAA TATGACACCC GGACAAAATA 
     2281 GAGAACAACG AGAAAGTGGC TTTTCATATC CAAATTTCAG TTTGCCTGCA GCCAATGGTT 
     2341 TATCTTCTGG AGTAGGTGGT GGAGGTGGCA AGATGAGACG AGAAAGAACA CGCTTTGTTG 
     2401 CTTCTAAACC TCTGGAGGAG GAGGAAATGG AAGTTCCAGT ATTACCGAAA ATCTCTCTAC 
     2461 CGATCACCAG TTCTTCACTG CCTACCTTTA ATTTTAGTTC CCCTGAGATC ACAACTTCCT 
     2521 CTCCATCACC CATCAATTCG TCTCAAGCAT TAACAAACAA GGTACAAATG ACCTCTCCGA 
     2581 GCAGCACTGG CAGTCCCATG TTTAAATTTT CATCTCCAAT CGTAAAATCT ACTGAGGCAA 
     2641 ATGTACTACC TCCATCATCT ATTGGATTTA CATTTAGTGT GCCTGTTGCA AAAACAGCAG 
     2701 AACTTTCTGG TTCTAGTAGT ACTTTAGAAC CAATTATAAG TAGTTCAGGT TTCGCATCGC 
     2761 CGAAGATAGA TTCTGTTGCT GCTCAGCCCA CCGCAACAAG CCCAGTAGTT TATACAAGAC 
     2821 CAGCAATAAG TAGCTTTTCT TCTAGTGGAA TTGGGTTTGG GGAGAGTTTA AAAGCTGGGT 
     2881 CATCATGGCA GTGTGATACA TGTCTACTCC AGAACAAAGT TACAGACAAC AAATGCATAG 
     2941 CCTGTCAAGC AGCAAAATTG TCACCCAGAG ATACTGCTAA ACAGACTGGA ATTGAAACAC 
     3001 CAAATAAAAG TGGCAAAACA ACTCTTTCTG CATCAGGGAC AGGCTTTGGA GACAAATTTA 
     3061 AACCAGTGAT AGGCACTTGG GATTGTGATA CCTGTTTAGT GCAAAATAAA CCTGAAGCAA 
     3121 TAAAATGTGT AGCCTGTGAA ACACCGAAAC CTGGAACTTG TGTGAAGCGA GCCCTTACAT 
     3181 TGACAGTGGT TTCGGAAAGT GCTGAGACTA TGACTGCTTC ATCTTCCAGC TGCACTGTAA 
     3241 CCACTGGTAC CTTAGGATTT GGAGATAAAT TCAAAAGGCC CATTGGATCT TGGGAGTGTT 
     3301 CAGTATGCTG TGTTTCTAAT AATGCAGAAG ACAATAAGTG TGTGTCCTGT ATGTCTGAGA 
     3361 AACCAGGAAG TTCAGTACCT GCTTCAAGTA GCAGCACTGT ACCTGTCTCT CTGCCTTCTG 
     3421 GAGGCTCTCT AGGATTGGAA AAGTTCAAGA AACCCGAGGG AAGCTGGGAC TGTGAATTGT 
     3481 GCCTAGTGCA GAATAAGGCA GACTCTACCA AATGTTTGGC ATGTGAAAGT GCAAAGCCAG 
     3541 GCACAAAATC TGGGTTTAAA GGCTTTGACA CATCTTCCTC ATCTTCGAAC TCAGCAGCCT 
     3601 CCTCATCCTT CAAATTTGGT GTCTCATCAT CCTCTTCTGG GCCTTCTCAG ACTTTAACAA 
     3661 GCACTGGAAA TTTTAAATTT GGAGATCAGG GAGGATTCAA AATAGGTGTG TCATCCGATT 
     3721 CTGGGTCTAT AAACCCCATG AGTGAAGGCT TTAAATTTTC TAAACCAATA GGAGATTTTA 
     3781 AATTTGGAGT TTCATCTGAA TCTAAGCCCG AAGAAGTTAA AAAAGATAGT AAGAATGATA 
     3841 ATTTTAAGTT TGGACTTTCT TCTGGTTTAA GCAACCCAGT TTCTTTAACT CCATTTCAAT 
     3901 TTGGGGTATC TAATCTTGGA CAGGAAGAAA AGAAAGAGGA ACTGCCCAAA TCTTCCTCTG 
     3961 CAGGTTTTAG CTTTGGTACA GGTGTTATTA ACTCCACCCC TGCTCCTGCT AACACCATAG 
     4021 TGACCTCTGA GAACAAGAGC AGCTTCAACC TTGGAACCAT AGAAACCAAG AGTGCTTCAG 
     4081 TGGCTCCTTT CACATGTAAG ACATCAGAAG CTAAAAAAGA AGAAATGCCT GCCACCAAAG 
     4141 GAGGATTCTC TTTTGGCAAC GTGGAGCCTG CCTCTCTGCC ATCTGCCTCA GTGTTTGTTT 
     4201 TGGGAAGGAC AGAAGAGAAA CAGCAAGAGC CTGTCACTTC TACTTCCCTA GTTTTTGGGA 
     4261 AGAAAGCTGA CAATGAAGAG CCAAAGTGTC AACCAGTGTT TTCCTTTGGG AATTCAGAGC 
     4321 AAACCAAAGA TGAGAATTCT TCAAAGTCCA CATTTAGTTT TAGTATGACA AAACCATCTG 
     4381 AGAAGGAATC TGAACAGCCA GCAAAAGCCA CTTTTGCCTT TGGAGCTCAA ACTAGTACTA 
     4441 CAGCTGATCA AGGTGCAGCA AAGCCAGTTT TTAGTTTCTT GAACAACAGT TCCTCTAGTT 
     4501 CAAGTACACC AGCCACTTCT GCTGGTGGTG GCATATTTGG TAGTTCCACC TCTTCCTCCA 
     4561 ATCCACCTGT GGCTACCTTT GTGTTTGGAC AGTCCAGCAA TCCTGTGAGC AGCTCTGCCT 
     4621 TTGGTAACAC TGCTGAATCC AGCACCTCTC AGTCTTTGCT ATTTTCTCAA GATAGCAAAC 
     4681 TAGCAACCAC ATCCAGCACA GGTACAGCTG TCACCCCATT TGTCTTTGGT CCAGGAGCCA 
     4741 GCAGTAATAA TACTACCACC TCTGGTTTCG GCTTTGGAGC CACAACCACA TCTAGCTCTG 
     4801 CAGGATCCTC CTTTGTATTT GGAACTGGAC CCTCAGCACC ATCTGCCAGT CCAGCATTTG 
     4861 GTGCTAACCA GACCCCAACA TTTGGACAAA GTCAAGGTGC CAGCCAGCCC AATCCCCCAG 
     4921 GCTTTGGATC TATATCATCT TCCACAGCAT TATTTCCCAC TGGTTCTCAG CCTGCACCAC 
     4981 CTACTTTTGG GACAGTGTCA AGCAGTAGCC AGCCCCCTGT GTTTGGACAG CAACCTAGTC 
     5041 AGTCTGCATT TGGCTCTGGA ACAACTCCTA ATTCTAGTTC GGCTTTCCAG TTTGGCAGCA 
     5101 GCACTACAAA TTTCAACTTC ACAAACAACA GTCCATCAGG AGTGTTCACA TTTGGTGCAA 
     5161 ATTCTAGCAC ACCTGCAGCC TCAGCCCAGC CTTCAGGCTC GGGGGGCTTT CCATTTAACC 
     5221 AGTCTCCAGC AGCATTTACA GTGGGGTCAA ATGGGAAAAA TGTGTTCTCT TCTTCTGGAA 
     5281 CTTCATTCTC TGGTCGCAAG ATAAAGACTG CTGTTAGACG CAGGAAAACG CGTACGCGGC 
     5341 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     5401 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     5461 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     5521 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     5581 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     5641 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     5701 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5761 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5821 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5881 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5941 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     6001 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     6061 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     6121 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     6181 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     6241 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     6301 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     6361 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     6421 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     6481 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     6541 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     6601 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     6661 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6721 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6781 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6841 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6901 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6961 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     7021 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     7081 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     7141 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     7201 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     7261 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     7321 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     7381 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     7441 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     7501 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     7561 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     7621 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     7681 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7741 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7801 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7861 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7921 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7981 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     8041 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     8101 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     8161 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     8221 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     8281 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     8341 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     8401 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     8461 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     8521 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     8581 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     8641 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     8701 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8761 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8821 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8881 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8941 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     9001 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     9061 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     9121 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//