LOCUS       RC240141                9171 bp    DNA     circular  SYN 
DEFINITION  DAPK1 (myc-DDK-tagged) - Human death-associated protein kinase 1 (DAPK1), transcript variant 4
ACCESSION   NM_001288731
VERSION     NM_001288731.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5328..5419 
                     /label="Myc-DDK"  
     misc_feature    5449..6034 
                     /label="PolyA signal"  
     misc_feature    5506..5525 
                     /label="Restriction Enzyme"  
     misc_feature    6310..6982 
                     /label="ColE1"  
     misc_feature    7412..8206 
                     /label="Kan/Neo"  
     misc_feature    8241..8543 
                     /label="SV40 ori"  
     misc_feature    8629..9106 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5310..5316 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5310 
                     /label="DAPK1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCGTGTTC AGGCAGGAAA ACGTGGATGA TTACTACGAC ACCGGCGAGG 
     1081 AACTTGGCAG TGGACAGTTT GCGGTTGTGA AGAAATGCCG TGAGAAAAGC ACCGGCCTCC 
     1141 AGTATGCCGC CAAATTCATC AAGAAAAGGA GGACTAAGTC CAGCCGGCGG GGTGTGAGCC 
     1201 GCGAGGACAT CGAGCGGGAG GTCAGCATCC TGAAGGAGAT CCAGCACCCC AATGTCATCA 
     1261 CCCTGCACGA GGTCTATGAG AACAAGACGG ACGTCATCCT GATCTTGGAA CTCGTTGCAG 
     1321 GTGGCGAGCT GTTTGACTTC TTAGCTGAAA AGGAATCTTT AACTGAAGAG GAAGCAACTG 
     1381 AATTTCTCAA ACAAATTCTT AATGGTGTTT ACTACCTGCA CTCCCTTCAA ATCGCCCACT 
     1441 TTGATCTTAA GCCTGAGAAC ATAATGCTTT TGGATAGAAA TGTCCCCAAA CCTCGGATCA 
     1501 AGATCATTGA CTTTGGGTTG GCCCATAAAA TTGACTTTGG AAATGAATTT AAAAACATAT 
     1561 TTGGGACTCC AGAGTTTGTC GCTCCTGAGA TAGTCAACTA TGAACCTCTT GGTCTTGAGG 
     1621 CAGATATGTG GAGTATCGGG GTAATAACCT ATATCCTCCT AAGTGGGGCC TCCCCATTTC 
     1681 TTGGAGACAC TAAGCAAGAA ACGTTAGCAA ATGTATCCGC TGTCAACTAC GAATTTGAGG 
     1741 ATGAATACTT CAGTAATACC AGTGCCCTAG CCAAAGATTT CATAAGAAGA CTTCTGGTCA 
     1801 AGGATCCAAA GAAGAGAATG ACAATTCAAG ATAGTTTGCA GCATCCCTGG ATCAAGCCTA 
     1861 AAGATACACA ACAGGCACTT AGTAGAAAAG CATCAGCAGT AAACATGGAG AAATTCAAGA 
     1921 AGTTTGCAGC CCGGAAAAAA TGGAAACAAT CCGTTCGCTT GATATCACTG TGCCAAAGAT 
     1981 TATCCAGGTC ATTCCTGTCC AGAAGTAACA TGAGTGTTGC CAGAAGCGAT GATACTCTGG 
     2041 ATGAGGAAGA CTCCTTTGTG ATGAAAGCCA TCATCCATGC CATCAACGAT GACAATGTCC 
     2101 CAGGCCTGCA GCACCTTCTG GGCTCATTAT CCAACTATGA TGTTAACCAA CCCAACAAGC 
     2161 ACGGGACACC TCCATTACTC ATTGCTGCTG GCTGTGGGAA TATTCAAATA CTACAGTTGC 
     2221 TCATTAAAAG AGGCTCGAGA ATCGATGTCC AGGATAAGGG CGGGTCCAAT GCCGTCTACT 
     2281 GGGCTGCTCG GCATGGCCAC GTCGATACCT TGAAATTTCT CAGTGAGAAC AAATGCCCTT 
     2341 TGGATGTGAA AGACAAGTCT GGAGAGATGG CCCTCCACGT GGCAGCTCGC TATGGCCATG 
     2401 CTGACGTGGC TCAGTTACTG TGCAGCTTCG GCTCAAATCC CAATATCCAG GACAAGGAAG 
     2461 AAGAAACCCC CCTGCACTGT GCTGCTTGGC ACGGCTATTA CTCTGTGGCC AAAGCCCTTT 
     2521 GTGAAGCCGG CTGTAACGTG AACATCAAGA ACCGAGAAGG AGAGACGCCC CTCCTGACAG 
     2581 CCTCTGCCAG GGGCTACCAC GACATCGTGG AGTGTCTGGC CGAACATGGA GCCGACCTTA 
     2641 ATGCTTGCGA CAAGGACGGA CACATTGCCC TTCATCTGGC TGTAAGACGG TGTCAGATGG 
     2701 AGGTAATCAA GACTCTCCTC AGCCAAGGGT GTTTCGTCGA TTATCAAGAC AGGCACGGCA 
     2761 ATACTCCCCT CCATGTGGCA TGTAAAGATG GCAACATGCC TATCGTGGTG GCCCTCTGTG 
     2821 AAGCAAACTG CAATTTGGAC ATCTCCAACA AGTATGGGCG AACGCCTCTG CACCTTGCGG 
     2881 CCAACAACGG AATCCTAGAC GTGGTCCGGT ATCTCTGTCT GATGGGAGCC AGCGTTGAGG 
     2941 CGCTGACCAC GGACGGAAAG ACGGCAGAAG ATCTTGCTAG ATCGGAACAG CACGAGCACG 
     3001 TAGCAGGTCT CCTTGCAAGA CTTCGAAAGG ATACGCACCG AGGACTCTTC ATCCAGCAGC 
     3061 TCCGACCCAC ACAGAACCTG CAGCCAAGAA TTAAGCTCAA GCTGTTTGGC CACTCGGGAT 
     3121 CCGGGAAAAC CACCCTTGTA GAATCTCTCA AGTGTGGGCT GCTGAGGAGC TTTTTCAGAA 
     3181 GGCGTCGGCC CAGACTGTCT TCCACCAACT CCAGCAGGTT CCCACCTTCA CCCCTGGCTT 
     3241 CTAAGCCCAC AGTCTCAGTG AGCATCAACA ACCTGTACCC AGGCTGCGAG AACGTGAGTG 
     3301 TGAGGAGCCG CAGCATGATG TTCGAGCCGG GTCTTACCAA AGGGATGCTG GAGGTGTTTG 
     3361 TGGCCCCGAC CCACCACCCG CACTGCTCGG CCGATGACCA GTCCACCAAG GCCATCGACA 
     3421 TCCAGAACGC TTATTTGAAT GGAGTTGGCG ATTTCAGCGT GTGGGAGTTC TCTGGAAATC 
     3481 CTGTGTATTT CTGCTGTTAT GACTATTTTG CTGCAAATGA TCCCACGTCA ATCCATGTTG 
     3541 TTGTCTTTAG TCTAGAAGAG CCCTATGAGA TCCAGCTGAA CCAAGTGATT TTCTGGCTCA 
     3601 GTTTCCTGAA GTCCCTTGTC CCAGTTGAAG AACCCATAGC CTTCGGTGGC AAGCTGAAGA 
     3661 ACCCACTCCA AGTTGTCCTG GTGGCCACCC ACGCTGACAT CATGAATGTT CCTCGACCGG 
     3721 CTGGAGGCGA GTTTGGATAT GACAAAGACA CATCGTTGCT GAAAGAGATT AGGAACAGGT 
     3781 TTGGAAATGA TCTTCACATT TCAAATAAGC TGTTTGTTCT GGATGCTGGG GCTTCTGGGT 
     3841 CAAAGGACAT GAAGGTACTT CGAAATCATC TGCAAGAAAT ACGAAGCCAG ATTGTTTCGG 
     3901 TCTGTCCTCC CATGACTCAC CTGTGTGAGA AAATCATCTC CACGCTGCCT TCCTGGAGGA 
     3961 AGCTCAATGG ACCCAACCAG CTGATGTCGC TGCAGCAGTT TGTGTACGAC GTGCAGGACC 
     4021 AGCTGAACCC CCTGGCCAGC GAGGAGGACC TCAGGCGCAT TGCTCAGCAG CTCCACAGCA 
     4081 CAGGCGAGAT CAACATCATG CAAAGTGAAA CAGTTCAGGA CGTGCTGCTC CTGGACCCCC 
     4141 GCTGGCTCTG CACAAACGTC CTGGGGAAGT TGCTGTCCGT GGAGACCCCA CGGGCGCTGC 
     4201 ACCACTACCG GGGCCGCTAC ACCGTGGAGG ACATCCAGCG CCTGGTGCCC GACAGCGACG 
     4261 TGGAGGAGCT GCTGCAGATC CTCGATGCCA TGGACATCTG CGCCCGGGAC CTGAGCAGCG 
     4321 GGACCATGGT GGACGTCCCA GCCCTGATCA AGACAGACAA CCTGCACCGC TCCTGGGCTG 
     4381 ATGAGGAGGA CGAGGTGATG GTGTATGGTG GCGTGCGCAT CGTGCCCGTG GAACACCTCA 
     4441 CCCCCTTCCC ATGTGGCATC TTTCACAAGG TCCAGGTGAA CCTGTGCCGG TGGATCCACC 
     4501 AGCAAAGCAC AGAGGGCGAC GCGGACATCC GCCTGTGGGT GAATGGCTGC AAGCTGGCCA 
     4561 ACCGTGGGGC CGAGCTGCTG GTGCTGCTGG TCAACCACGG CCAGGGCATT GAGGTCCAGG 
     4621 TCCGCGGCCT GGAGACGGAG AAGATCAAGT GCTGCCTGCT GCTGGACTCG GTGTGCAGCA 
     4681 CCATTGAGAA CGTCATGGCC ACCACGCTGC CAGGGCTCCT GACCGTGAAG CATTACCTGA 
     4741 GCCCCCAGCA GCTGCGGGAG CACCATGAGC CCGTCATGAT CTACCAGCCA CGGGACTTCT 
     4801 TCCGGGCACA GACTCTGAAG GAAACCTCAC TGACCAACAC CATGGGGGGG TACAAGGAAA 
     4861 GCTTCAGCAG CATCATGTGC TTCGGGTGTC ACGACGTCTA CTCACAGGCC AGCCTCGGCA 
     4921 TGGACATCCA TGCATCAGAC CTGAACCTCC TCACTCGGAG GAAACTGAGT CGCCTGCTGG 
     4981 ACCCGCCCGA CCCCCTGGGG AAGGACTGGT GCCTTCTCGC CATGAACTTA GGCCTCCCTG 
     5041 ACCTCGTGGC AAAGTACAAC ACCAGTAACG GGGCTCCCAA GGATTTCCTC CCCAGCCCCC 
     5101 TCCACGCCCT GCTGCGGGAA TGGACCACCT ACCCTGAGAG CACAGTGGGC ACCCTCATGT 
     5161 CCAAACTGAG GGAGCTGGGT CGCCGGGATG CCGCAGACTT TTTGCTGAAG GCATCCTCTG 
     5221 TGTTCAAAAT CAACCTGGAT GGCAATGGCC AGGAGGCCTA TGCCTCGAGC TGCAACAGCG 
     5281 GCACCTCTTA CAATTCCATT AGCTCTGTTG TATCCCGGAC GCGTACGCGG CCGCTCGAGC 
     5341 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     5401 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     5461 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     5521 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     5581 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     5641 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5701 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5761 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5821 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5881 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5941 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     6001 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     6061 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     6121 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     6181 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     6241 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     6301 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     6361 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     6421 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     6481 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     6541 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     6601 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6661 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6721 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6781 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6841 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6901 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6961 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     7021 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     7081 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     7141 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     7201 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     7261 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     7321 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     7381 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     7441 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     7501 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     7561 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     7621 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7681 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7741 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7801 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7861 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7921 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7981 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     8041 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     8101 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     8161 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     8221 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     8281 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     8341 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     8401 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8461 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     8521 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     8581 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     8641 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8701 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8761 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8821 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8881 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8941 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     9001 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     9061 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     9121 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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