LOCUS       RC240137                9153 bp    DNA     circular  SYN 
DEFINITION  FANCA (myc-DDK-tagged) - Human Fanconi anemia, complementation group A (FANCA), transcript variant 3
ACCESSION   NM_001286167
VERSION     NM_001286167.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5310..5401 
                     /label="Myc-DDK"  
     misc_feature    5431..6016 
                     /label="PolyA signal"  
     misc_feature    5488..5507 
                     /label="Restriction Enzyme"  
     misc_feature    6292..6964 
                     /label="ColE1"  
     misc_feature    7394..8188 
                     /label="Kan/Neo"  
     misc_feature    8223..8525 
                     /label="SV40 ori"  
     misc_feature    8611..9088 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5292..5298 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5292 
                     /label="FANCA"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCGACTCG TGGGTCCCGA ACTCCGCCTC GGGCCAGGAC CCAGGGGGCC 
     1081 GCCGGAGGGC CTGGGCCGAG CTGCTGGCGG GAAGGGTCAA GAGGGAAAAA TATAATCCTG 
     1141 AAAGGGCACA GAAATTAAAG GAATCAGCTG TGCGCCTCCT GCGAAGCCAT CAGGACCTGA 
     1201 ATGCCCTTTT GCTTGAGGTA GAAGGTCCAC TGTGTAAAAA ATTGTCTCTC AGCAAAGTGA 
     1261 TTGACTGTGA CAGTTCTGAG GCCTATGCTA ATCATTCTAG TTCATTTATA GGCTCTGCTT 
     1321 TGCAGGATCA AGCCTCAAGG CTGGGGGTTC CCGTGGGTAT TCTCTCAGCC GGGATGGTTG 
     1381 CCTCTAGCGT GGGACAGATC TGCACGGCTC CAGCGGAGAC CAGTCACCCT GTGCTGCTGA 
     1441 CTGTGGAGCA GAGAAAGAAG CTGTCTTCCC TGTTAGAGTT TGCTCAGTAT TTATTGGCAC 
     1501 ACAGTATGTT CTCCCGTCTT TCCTTCTGTC AAGAATTATG GAAAATACAG AGTTCTTTGT 
     1561 TGCTTGAAGC GGTGTGGCAT CTTCACGTAC AAGGCATTGT GAGCCTGCAA GAGCTGCTGG 
     1621 AAAGCCATCC CGACATGCAT GCTGTGGGAT CGTGGCTCTT CAGGAATCTG TGCTGCCTTT 
     1681 GTGAACAGAT GGAAGCATCC TGCCAGCATG CTGACGTCGC CAGGGCCATG CTTTCTGATT 
     1741 TTGTTCAAAT GTTTGTTTTG AGGGGATTTC AGAAAAACTC AGATCTGAGA AGAACTGTGG 
     1801 AGCCTGAAAA AATGCCGCAG GTCACGGTTG ATGTACTGCA GAGAATGCTG ATTTTTGCAC 
     1861 TTGACGCTTT GGCTGCTGGA GTACAGGAGG AGTCCTCCAC TCACAAGATC GTGAGGTGCT 
     1921 GGTTCGGAGT GTTCAGTGGA CACACGCTTG GCAGTGTAAT TTCCACAGAT CCTCTGAAGA 
     1981 GGTTCTTCAG TCATACCCTG ACTCAGATAC TCACTCACAG CCCTGTGCTG AAAGCATCTG 
     2041 ATGCTGTTCA GATGCAGAGA GAGTGGAGCT TTGCGCGGAC ACACCCTCTG CTCACCTCAC 
     2101 TGTACCGCAG GCTCTTTGTG ATGCTGAGTG CAGAGGAGTT GGTTGGCCAT TTGCAAGAAG 
     2161 TTCTGGAAAC GCAGGAGGTT CACTGGCAGA GAGTGCTCTC CTTTGTGTCT GCCCTGGTTG 
     2221 TCTGCTTTCC AGAAGCGCAG CAGCTGCTTG AAGACTGGGT GGCGCGTTTG ATGGCCCAGG 
     2281 CATTCGAGAG CTGCCAGCTG GACAGCATGG TCACTGCGTT CCTGGTTGTG CGCCAGGCAG 
     2341 CACTGGAGGG CCCCTCTGCG TTCCTGTCAT ATGCAGACTG GTTCAAGGCC TCCTTTGGGA 
     2401 GCACACGAGG CTACCATGGC TGCAGCAAGA AGGCCCTGGT CTTCCTGTTT ACGTTCTTGT 
     2461 CAGAACTCGT GCCTTTTGAG TCTCCCCGGT ACCTGCAGGT GCACATTCTC CACCCACCCC 
     2521 TGGTTCCCGG CAAGTACCGC TCCCTCCTCA CAGACTACAT CTCATTGGCC AAGACACGGC 
     2581 TGGCCGACCT CAAGGTTTCT ATAGAAAACA TGGGACTCTA CGAGGATTTG TCATCAGCTG 
     2641 GGGACATTAC TGAGCCCCAC AGCCAAGCTC TTCAGGATGT TGAAAAGGCC ATCATGGTGT 
     2701 TTGAGCATAC GGGGAACATC CCAGTCACCG TCATGGAGGC CAGCATATTC AGGAGGCCTT 
     2761 ACTACGTGTC CCACTTCCTC CCCGCCCTGC TCACACCTCG AGTGCTCCCC AAAGTCCCTG 
     2821 ACTCCCGTGT GGCGTTTATA GAGTCTCTGA AGAGAGCAGA TAAAATCCCC CCATCTCTGT 
     2881 ACTCCACCTA CTGCCAGGCC TGCTCTGCTG CTGAAGAGAA GCCAGAAGAT GCAGCCCTGG 
     2941 GAGTGAGGGC AGAACCCAAC TCTGCTGAGG AGCCCCTGGG ACAGCTCACA GCTGCACTGG 
     3001 GAGAGCTGAG AGCCTCCATG ACAGACCCCA GCCAGCGTGA TGTTATATCG GCACAGGTGG 
     3061 CAGTGATTTC TGAAAGACTG AGGGCTGTCC TGGGCCACAA TGAGGATGAC AGCAGCGTTG 
     3121 AGATATCAAA GATTCAGCTC AGCATCAACA CGCCGAGACT GGAGCCACGG GAACACATGG 
     3181 CTGTGGACCT CCTGCTGACG TCTTTCTGTC AGAACCTGAT GGCTGCCTCC AGTGTCGCTC 
     3241 CCCCGGAGAG GCAGGGTCCC TGGGCTGCCC TCTTCGTGAG GACCATGTGT GGACGTGTGC 
     3301 TCCCTGCAGT GCTCACCCGG CTCTGCCAGC TGCTCCGTCA CCAGGGCCCG AGCCTGAGTG 
     3361 CCCCACATGT GCTGGGGTTG GCTGCCCTGG CCGTGCACCT GGGTGAGTCC AGGTCTGCGC 
     3421 TCCCAGAGGT GGATGTGGGT CCTCCTGCAC CTGGTGCTGG CCTTCCTGTC CCTGCGCTCT 
     3481 TTGACAGCCT CCTGACCTGT AGGACGAGGG ATTCCTTGTT CTTCTGCCTG AAATTTTGTA 
     3541 CAGCAGCAAT TTCTTACTCT CTCTGCAAGT TTTCTTCCCA GTCACGAGAT ACTTTGTGCA 
     3601 GCTGCTTATC TCCAGGCCTT ATTAAAAAGT TTCAGTTCCT CATGTTCAGA TTGTTCTCAG 
     3661 AGGCCCGACA GCCTCTTTCT GAGGAGGACG TAGCCAGCCT TTCCTGGAGA CCCTTGCACC 
     3721 TTCCTTCTGC AGACTGGCAG AGAGCTGCCC TCTCTCTCTG GACACACAGA ACCTTCCGAG 
     3781 AGGTGTTGAA AGAGGAAGAT GTTCACTTAA CTTACCAAGA CTGGTTACAC CTGGAGCTGG 
     3841 AAATTCAACC TGAAGCTGAT GCTCTTTCAG ATACTGAACG GCAGGACTTC CACCAGTGGG 
     3901 CGATCCATGA GCACTTTCTC CCTGAGTCCT CGGCTTCAGG GGGCTGTGAC GGAGACCTGC 
     3961 AGGCTGCGTG TACCATTCTT GTCAACGCAC TGATGGATTT CCACCAAAGC TCAAGGAGTT 
     4021 ATGACCACTC AGAAAATTCT GATTTGGTCT TTGGTGGCCG CACAGGAAAT GAGGATATTA 
     4081 TTTCCAGATT GCAGGAGATG GTAGCTGACC TGGAGCTGCA GCAAGACCTC ATAGTGCCTC 
     4141 TCGGCCACAC CCCTTCCCAG GAGCACTTCC TCTTTGAGAT TTTCCGCAGA CGGCTCCAGG 
     4201 CTCTGACAAG CGGGTGGAGC GTGGCTGCCA GCCTTCAGAG ACAGAGGGAG CTGCTAATGT 
     4261 ACAAACGGAT CCTCCTCCGC CTGCCTTCGT CTGTCCTCTG CGGCAGCAGC TTCCAGGCAG 
     4321 AACAGCCCAT CACTGCCAGA TGCGAGCAGT TCTTCCACTT GGTCAACTCT GAGATGAGAA 
     4381 ACTTCTGCTC CCACGGAGGT GCCCTGACAC AGGACATCAC TGCCCACTTC TTCAGGGGCC 
     4441 TCCTGAACGC CTGTCTGCGG AGCAGAGACC CCTCCCTGAT GGTCGACTTC ATACTGGCCA 
     4501 AGTGCCAGAC GAAATGCCCC TTAATTTTGA CCTCTGCTCT GGTGTGGTGG CCGAGCCTGG 
     4561 AGCCTGTGCT GCTCTGCCGG TGGAGGAGAC ACTGCCAGAG CCCGCTGCCC CGGGAACTGC 
     4621 AGAAGCTACA AGAAGGCCGG CAGTTTGCCA GCGATTTCCT CTCCCCTGAG GCTGCCTCCC 
     4681 CAGCACCCAA CCCGGACTGG CTCTCAGCTG CTGCACTGCA CTTTGCGATT CAACAAGTCA 
     4741 GGGAAGAAAA CATCAGGAAG CAGCTAAAGA AGCTGGACTG CGAGAGAGAG GAGCTATTGG 
     4801 TTTTCCTTTT CTTCTTCTCC TTGATGGGCC TGCTGTCGTC ACATCTGACC TCAAATAGCA 
     4861 CCACAGACCT GCCAAAGGCT TTCCACGTTT GTGCAGCAAT CCTCGAGTGT TTAGAGAAGA 
     4921 GGAAGATATC CTGGCTGGCA CTCTTTCAGT TGACAGAGAG TGACCTCAGG CTGGGGCGGC 
     4981 TCCTCCTCCG TGTGGCCCCG GATCAGCACA CCAGGCTGCT GCCTTTCGCT TTTTACAGTC 
     5041 TTCTCTCCTA CTTCCATGAA GACGCGGCCA TCAGGGAAGA GGCCTTCCTG CATGTTGCTG 
     5101 TGGACATGTA CTTGAAGCTG GTCCAGCTCT TCGTGGCTGG GGATACAAGC ACAGTTTCAC 
     5161 CTCCAGCTGG CAGGAGCCTG GAGCTCAAGG GTCAGGCAGG GCAACCCCGT GGAACTGATA 
     5221 ACAAAAGCTC GTCTTTTTCT GCTGCAGTTA ATACCTCGGT GCCCGAAAAA GAGCTTCTCA 
     5281 CACGTGGCAG AGCTGCTGGC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     5341 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     5401 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     5461 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     5521 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5581 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5641 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5701 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5761 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5821 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5881 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5941 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     6001 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     6061 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     6121 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     6181 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     6241 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     6301 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     6361 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     6421 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     6481 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6541 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6601 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6661 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6721 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6781 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6841 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6901 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6961 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     7021 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     7081 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     7141 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     7201 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     7261 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     7321 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     7381 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     7441 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     7501 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7561 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7621 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7681 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7741 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7801 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7861 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7921 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7981 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     8041 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     8101 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     8161 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     8221 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     8281 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     8341 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     8401 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8461 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8521 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8581 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8641 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8701 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8761 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8821 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8881 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8941 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     9001 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     9061 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     9121 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//