LOCUS       RC240136                9147 bp    DNA     circular  SYN 
DEFINITION  ATP7A (myc-DDK-tagged) - Human ATPase, Cu++ transporting, alpha polypeptide (ATP7A), transcript variant 2
ACCESSION   NM_001282224
VERSION     NM_001282224.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5304..5395 
                     /label="Myc-DDK"  
     misc_feature    5425..6010 
                     /label="PolyA signal"  
     misc_feature    5482..5501 
                     /label="Restriction Enzyme"  
     misc_feature    6286..6958 
                     /label="ColE1"  
     misc_feature    7388..8182 
                     /label="Kan/Neo"  
     misc_feature    8217..8519 
                     /label="SV40 ori"  
     misc_feature    8605..9082 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5286..5292 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5286 
                     /label="ATP7A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATCCAAGT ATGGGTGTGA ATTCTGTTAC CATTTCTGTT GAGGGTATGA 
     1081 CTTGCAATTC CTGTGTTTGG ACCATTGAGC AGCAGATTGG AAAAGTGAAT GGTGTGCATC 
     1141 ACATTAAGGT ATCACTGGAA GAAAAAAATG CAACTATTAT TTATGACCCT AAACTACAGA 
     1201 CTCCAAAGAC CCTACAGGAA GCTATTGATG ACATGGGCTT TGATGCTGTT ATCCATAATC 
     1261 CTGACCCTCT CCCTGTTTTA ACTGACACCT TGTTTCTGAC TGTTACGGCG TCACTGACTT 
     1321 TGCCATGGGA CCATATCCAA AGCACATTGC TGAAGACCAA GGGTGTGACA GACATTAAAA 
     1381 TTTACCCTCA GAAAAGAACT GTAGCAGTGA CAATAATCCC TTCTATAGTG AATGCCAATC 
     1441 AGATAAAAGA GCTGGTTCCA GAACTCAGTT TAGATACTGG GACACTGGAG AAAAAGTCAG 
     1501 GAGCTTGTGA AGATCATAGT ATGGCTCAAG CTGGTGAAGT CGTGCTGAAG ATGAAAGTGG 
     1561 AAGGGATGAC CTGCCATTCA TGTACTAGCA CTATTGAAGG AAAAATTGGG AAACTGCAAG 
     1621 GTGTTCAGCG AATTAAAGTC TCCCTGGACA ATCAAGAAGC TACTATTGTT TATCAACCTC 
     1681 ATCTTATCTC AGTAGAGGAA ATGAAAAAGC AGATTGAAGC TATGGGCTTT CCAGCATTTG 
     1741 TCAAAAAGCA GCCCAAGTAC CTCAAATTGG GAGCTATTGA TGTAGAACGT CTAAAGAACA 
     1801 CACCAGTTAA ATCCTCAGAA GGGTCACAGC AAAGGAGTCC ATCATATACC AATGATTCAA 
     1861 CAGCCACTTT CATCATTGAT GGCATGCATT GTAAATCATG TGTGTCAAAT ATTGAAAGTA 
     1921 CTTTATCTGC ACTCCAATAT GTAAGCAGCA TAGTAGTTTC TTTAGAGAAT AGGTCTGCCA 
     1981 TTGTGAAGTA TAATGCAAGC TCAGTCACTC CAGAATCCCT GAGAAAAGCA ATAGAGGCTG 
     2041 TATCACCGGG GCTATATAGA GTTAGTATCA CAAGTGAAGT TGAGAGTACC TCAAACTCTC 
     2101 CCTCCAGCTC ATCTCTTCAG AAGATTCCTT TGAATGTAGT TAGCCAGCCT CTGACACAAG 
     2161 AAACTGTGAT AAACATTGAT GGCATGACTT GTAATTCCTG TGTGCAGTCT ATTGAGGGTG 
     2221 TCATATCAAA AAAGCCAGGT GTAAAATCCA TACGAGTCTC CCTTGCAAAT AGCAATGGGA 
     2281 CTGTTGAGTA TGATCCTCTA CTAACCTCTC CAGAAACGTT GAGAGGAGCA ATAGAAGACA 
     2341 TGGGATTTGA TGCTACCTTG TCAGACACGA ATGAGCCGTT GGTAGTAATA GCTCAGCCTT 
     2401 CATCGGAAAT GCCGCTTTTG ACTTCAACTA ATGAATTTTA TACTAAAGGG ATGACACCAG 
     2461 TTCAAGACAA GGAGGAAGGA AAGAATTCAT CTAAGTGTTA CATACAGGTC ACTGGCATGA 
     2521 CTTGCGCTTC CTGTGTAGCA AACATTGAAC GGAATTTAAG GCGGGAAGAA GGAATATATT 
     2581 CTATACTTGT GGCCCTGATG GCTGGCAAGG CAGAAGTAAG GTATAATCCT GCTGTTATAC 
     2641 AACCCCCAAT GATAGCAGAG TTCATCCGAG AACTTGGATT TGGAGCCACT GTGATAGAAA 
     2701 ATGCTGATGA AGGAGATGGT GTTTTGGAAC TTGTTGTGAG GGGAATGACG TGTGCCTCCT 
     2761 GCGTACATAA AATAGAGTCT AGTCTCACAA AACACAGAGG GATCCTATAC TGCTCCGTGG 
     2821 CCCTGGCAAC CAACAAAGCA CATATTAAAT ATGACCCAGA AATTATTGGT CCTAGAGATA 
     2881 TTATCCATAC AATTGAAAGC TTAGGTTTTG AAGCTTCTTT GGTCAAGAAG GATCGGTCAG 
     2941 CAAGTCACTT AGATCATAAA CGAGAAATAA GACAATGGAG ACGGTCTTTT CTTGTGAGTC 
     3001 TGTTTTTCTG TATTCCTGTA ATGGGGCTGA TGATATATAT GATGGTTATG GACCACCACT 
     3061 TTGCAACTCT TCACCATAAT CAAAACATGA GTAAAGAAGA AATGATCAAC CTTCATTCTT 
     3121 CTATGTTCCT GGAGCGCCAG ATTCTTCCAG GATTGTCTGT TATGAATTTG CTGTCCTTTT 
     3181 TATTGTGTGT ACCTGTACAG GGCAAAACAT CAGAGGCTCT TGCAAAGTTA ATTTCACTAC 
     3241 AAGCTACAGA AGCAACTATT GTAACTCTTG ATTCTGATAA TATCCTCCTC AGTGAAGAAC 
     3301 AAGTGGATGT GGAACTTGTA CAACGTGGAG ATATCATTAA AGTAGTTCCA GGAGGCAAAT 
     3361 TTCCAGTGGA TGGTCGTGTT ATTGAAGGAC ATTCTATGGT AGATGAGTCC CTCATCACAG 
     3421 GGGAGGCAAT GCCTGTGGCT AAGAAACCTG GCAGCACAGT GATTGCTGGT TCCATTAACC 
     3481 AGAACGGGTC ACTGCTTATC TGCGCAACAC ATGTTGGAGC AGACACAACC CTTTCTCAAA 
     3541 TTGTCAAACT TGTGGAAGAG GCACAAACAT CAAAGGCTCC TATCCAGCAG TTTGCAGACA 
     3601 AACTCAGTGG CTATTTTGTT CCTTTTATTG TTTTTGTTTC CATTGCCACC CTCTTGGTAT 
     3661 GGATTGTAAT TGGATTTCTG AATTTTGAAA TTGTGGAAAC CTACTTTCCT GGCTACAATA 
     3721 GAAGTATCTC CCGAACAGAA ACGATAATAC GATTTGCTTT CCAAGCCTCT ATCACAGTTC 
     3781 TGTGTATTGC ATGTCCCTGT TCACTGGGAC TGGCCACTCC AACTGCTGTG ATGGTGGGTA 
     3841 CAGGAGTAGG TGCTCAAAAT GGCATACTAA TAAAAGGTGG AGAGCCATTG GAGATGGCTC 
     3901 ATAAGGTAAA GGTAGTGGTA TTTGATAAGA CTGGAACCAT TACTCACGGA ACCCCAGTGG 
     3961 TGAATCAAGT AAAGGTTCTA ACTGAAAGTA ACAGAATATC ACACCATAAA ATCTTGGCCA 
     4021 TTGTGGGAAC TGCTGAAAGT AACAGTGAAC ACCCTCTAGG AACAGCCATA ACCAAATATT 
     4081 GCAAACAGGA GCTGGACACT GAAACCTTGG GTACCTGCAT AGATTTCCAG GTTGTGCCAG 
     4141 GCTGTGGTAT TAGCTGTAAA GTCACCAATA TTGAAGGCTT GCTACATAAG AATAACTGGA 
     4201 ATATAGAGGA CAATAATATT AAAAATGCAT CCCTGGTTCA AATTGATGCC AGTAATGAAC 
     4261 AGTCATCAAC TTCGTCTTCC ATGATTATTG ATGCCCAGAT CTCAAATGCT CTTAATGCTC 
     4321 AGCAGTATAA AGTCCTCATT GGTAACCGGG AGTGGATGAT TAGAAATGGT CTTGTCATTA 
     4381 ATAACGATGT AAATGATTTC ATGACTGAAC ATGAGAGAAA AGGTCGGACT GCTGTATTAG 
     4441 TAGCAGTTGA TGATGAGCTG TGTGGCTTGA TAGCCATTGC AGACACAGTG AAGCCTGAAG 
     4501 CAGAACTGGC TATCCATATT CTGAAATCTA TGGGCTTAGA AGTAGTTCTG ATGACTGGAG 
     4561 ACAACAGTAA AACAGCTAGA TCTATTGCTT CTCAGGTTGG CATTACTAAG GTGTTTGCTG 
     4621 AAGTTCTACC TTCTCACAAG GTTGCTAAAG TGAAGCAACT TCAAGAGGAG GGGAAACGGG 
     4681 TAGCAATGGT GGGAGATGGA ATCAATGACT CCCCAGCTCT GGCAATGGCT AATGTGGGAA 
     4741 TTGCTATTGG CACAGGCACA GATGTAGCCA TTGAAGCAGC TGATGTGGTT TTGATAAGGA 
     4801 ATGATCTTCT GGATGTAGTG GCAAGTATTG ACTTATCAAG AAAGACAGTC AAGAGGATTC 
     4861 GGATAAATTT TGTCTTTGCT CTAATTTATA ATCTGGTTGG AATTCCCATA GCTGCTGGAG 
     4921 TTTTTATGCC CATTGGTTTG GTTTTGCAGC CCTGGATGGG ATCTGCAGCA ATGGCTGCTT 
     4981 CATCTGTTTC TGTAGTACTT TCTTCTCTCT TCCTTAAACT TTACAGGAAA CCAACTTACG 
     5041 AGAGTTATGA ACTGCCTGCC CGGAGCCAGA TAGGACAGAA GAGTCCTTCA GAAATCAGCG 
     5101 TTCATGTTGG AATAGATGAT ACCTCAAGGA ATTCTCCTAA ACTGGGTTTG CTGGACCGGA 
     5161 TTGTTAATTA TAGCAGAGCC TCTATAAACT CACTACTGTC TGATAAACGC TCCCTAAACA 
     5221 GTGTTGTTAC CAGTGAACCT GACAAGCACT CACTCCTGGT GGGAGACTTC AGGGAAGATG 
     5281 ATGACACTGC ATTAACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     5341 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     5401 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     5461 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     5521 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     5581 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5641 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5701 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5761 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5821 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5881 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5941 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     6001 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     6061 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     6121 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     6181 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     6241 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     6301 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     6361 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     6421 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     6481 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     6541 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6601 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6661 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6721 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6781 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6841 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6901 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6961 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     7021 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     7081 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     7141 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     7201 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     7261 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     7321 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     7381 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     7441 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     7501 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     7561 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7621 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7681 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7741 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7801 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7861 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7921 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7981 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     8041 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     8101 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     8161 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     8221 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     8281 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     8341 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     8401 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8461 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8521 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     8581 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8641 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8701 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8761 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8821 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8881 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8941 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     9001 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     9061 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     9121 ATTTAACAAA AATTTAACGC GAATTTT
//