LOCUS       RC240132                9105 bp    DNA     circular  SYN 
DEFINITION  KDM6A (myc-DDK-tagged) - Human lysine (K)-specific demethylase 6A (KDM6A), transcript variant 2
ACCESSION   NM_001291416
VERSION     NM_001291416.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5262..5353 
                     /label="Myc-DDK"  
     misc_feature    5383..5968 
                     /label="PolyA signal"  
     misc_feature    5440..5459 
                     /label="Restriction Enzyme"  
     misc_feature    6244..6916 
                     /label="ColE1"  
     misc_feature    7346..8140 
                     /label="Kan/Neo"  
     misc_feature    8175..8477 
                     /label="SV40 ori"  
     misc_feature    8563..9040 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5244..5250 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5244 
                     /label="KDM6A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAATCCTGC GGAGTGTCGC TCGCTACCGC CGCCGCTGCC GCCGCCGCTT 
     1081 TCGGTGATGA GGAAAAGAAA ATGGCGGCGG GAAAAGCGAG CGGCGAGAGC GAGGAGGCGT 
     1141 CCCCCAGCCT GACAGCCGAG GAGAGGGAGG CGCTCGGCGG ACTGGACAGC CGCCTCTTTG 
     1201 GGTTCGTGAG ATTTCATGAA GATGGCGCCA GGACGAAGGC CCTACTGGGC AAGGCTGTTC 
     1261 GCTGCTATGA ATCTCTAATC TTAAAAGCTG AAGGAAAAGT GGAGTCTGAT TTCTTTTGTC 
     1321 AATTAGGTCA CTTCAACCTC TTATTGGAAG ATTATCCAAA AGCATTATCT GCATACCAGA 
     1381 GGTACTACAG TTTACAGTCT GACTACTGGA AGAATGCTGC CTTTTTATAT GGTCTTGGTT 
     1441 TGGTCTACTT CCATTATAAT GCATTTCAGT GGGCAATTAA AGCATTTCAG GAGGTGCTTT 
     1501 ATGTTGATCC CAGCTTTTGT CGAGCCAAGG AAATTCATTT ACGACTTGGG CTTATGTTCA 
     1561 AAGTGAACAC AGACTATGAG TCTAGTTTAA AGCATTTTCA GTTAGCTTTG GTTGACTGTA 
     1621 ATCCCTGCAC TTTGTCCAAT GCTGAAATTC AATTTCACAT TGCCCACTTA TATGAAACCC 
     1681 AGAGGAAATA TCATTCTGCA AAAGAAGCTT ATGAACAACT TTTGCAGACA GAGAATCTTT 
     1741 CTGCACAAGT AAAAGCAACT GTCTTACAAC AGTTAGGTTG GATGCATCAC ACTGTAGATC 
     1801 TCCTGGGAGA TAAAGCCACC AAGGAAAGCT ATGCTATTCA GTATCTCCAA AAGTCCTTGG 
     1861 AAGCAGATCC TAATTCTGGC CAGTCCTGGT ATTTCCTCGG AAGGTGCTAT TCAAGTATTG 
     1921 GGAAAGTTCA GGATGCCTTT ATATCTTACA GGCAGTCTAT TGATAAATCA GAAGCAAGTG 
     1981 CAGATACATG GTGTTCAATA GGTGTGCTAT ATCAGCAGCA AAATCAGCCC ATGGATGCTT 
     2041 TACAGGCCTA TATTTGTGCT GTACAATTGG ACCATGGCCA TGCTGCAGCC TGGATGGACC 
     2101 TAGGCACTCT CTATGAATCC TGCAACCAGC CTCAGGATGC CATTAAATGC TACTTAAATG 
     2161 CAACTAGAAG CAAAAGTTGT AGTAATACCT CTGCACTTGC AGCACGAATT AAGTATTTAC 
     2221 AGGCATGTAA ACCTCATCAT CCAAATACTG AACCTGTATT AGGCCTCAGT CAAACACCAA 
     2281 TTTCACAGCA ATCCTTGCCA CTACACATGA TTCCTTCTAG CCAAGTAGAT GACCTGTCCA 
     2341 GTCCTGCCAA GAGGAAAAGA ACATCTAGTC CAACAAAGAA TACTTCTGAC AATTGGAGTG 
     2401 GTGGACATGC TGTGTCACAT CCTCCAGTAC AGCAACAAGC TCATTCATGG TGTTTGACAC 
     2461 CACAGAAATT ACAGCATTTG GAACAGCTCC GCGCAAATAG AAATAATTTA AATCCAGCAC 
     2521 AGAAACTGAT GCTGGAACAG CTGGAAAGTC AGTTTGTCTT AATGCAACAA CACCAAATGA 
     2581 GACCAACAGG AGTTGCACAG GTACGATCTA CTGGAATTCC TAATGGGCCA ACAGCTGACT 
     2641 CATCACTGCC TACAAACTCA GTCTCTGGCC AGCAGCCACA GCTTGCTCTG ACCAGAGTGC 
     2701 CTAGCGTCTC TCAGCCTGGA GTCCGTCCTG CCTGCCCTGG GCAGCCTTTG GCCAATGGAC 
     2761 CCTTTTCTGC AGGCCATGTT CCCTGTAGCA CATCAAGAAC GCTGGGAAGT ACAGACACTA 
     2821 TTTTGATAGG CAATAATCAT ATAACAGGAA GTGGAAGTAA TGGAAACGTG CCTTACCTGC 
     2881 AGCGAAACGC ACTCACTCTA CCTCATAACC GCACAAACCT GACCAGCAGC GCAGAGGAGC 
     2941 CGTGGAAAAA CCAACTATCT AACTCCACTC AGGGGCTTCA CAAAGGTCAG AGTTCACATT 
     3001 CGGCAGGTCC TAATGGTGAA CGACCTCTCT CTTCCACTGG GCCTTCCCAG CATCTCCAGG 
     3061 CAGCTGGCTC TGGTATTCAG AATCAGAACG GACATCCCAC CCTGCCTAGC AATTCAGTAA 
     3121 CACAGGGGGC TGCTCTCAAT CACCTCTCCT CTCACACTGC TACCTCAGGT GGACAACAAG 
     3181 GCATTACCTT AACCAAAGAG AGCAAGCCTT CAGGAAACAT ATTGACGGTG CCTGAAACAA 
     3241 GCAGGCACAC TGGAGAGACA CCTAACAGCA CTGCCAGTGT CGAGGGACTT CCTAATCATG 
     3301 TCCATCAGAT GACGGCAGAT GCTGTTTGCA GTCCTAGCCA TGGAGATTCT AAGTCACCAG 
     3361 GTTTACTAAG TTCAGACAAT CCTCAGCTCT CTGCCTTGTT GATGGGAAAA GCCAATAACA 
     3421 ATGTGGGTAC TGGAACCTGT GACAAAGTCA ATAACATCCA CCCAGCTGTT CATACAAAGA 
     3481 CTGATAACTC TGTTGCCTCT TCACCATCTT CAGCCATTTC AACAGCAACA CCTTCTCCAA 
     3541 AATCCACTGA GCAGACAACC ACAAACAGTG TTACCAGCCT TAACAGCCCT CACAGTGGGC 
     3601 TACACACAAT TAATGGAGAA GGGATGGAAG AATCTCAGAG CCCCATGAAA ACAGATCTGC 
     3661 TTCTGGTTAA CCACAAACCT AGTCCACAGA TCATACCATC AATGTCTGTG TCCATATACC 
     3721 CCAGCTCAGC AGAAGTTCTG AAGGCATGCA GGAATCTAGG TAAAAATGGC TTATCTAACA 
     3781 GTAGCATTTT GTTGGATAAA TGTCCACCTC CAAGACCACC ATCTTCACCA TACCCTCCCT 
     3841 TGCCAAAGGA CAAGTTGAAT CCACCTACAC CTAGTATTTA CTTGGAAAAT AAACGTGATG 
     3901 CTTTCTTTCC TCCATTACAT CAATTTTGTA CAAATCCGAA CAACCCTGTT ACAGTAATAC 
     3961 GTGGCCTTGC TGGAGCTCTT AAGTTAGACC TGGGACTTTT CTCTACTAAA ACTTTGGTGG 
     4021 AAGCTAACAA TGAACATATG GTAGAAGTGA GGACACAGTT GTTGCAGCCA GCAGATGAAA 
     4081 ACTGGGATCC CACTGGAACA AAGAAAATCT GGCATTGTGA AAGTAATAGA TCTCATACTA 
     4141 CAATTGCTAA ATATGCACAG TACCAGGCCT CCTCATTCCA GGAATCATTG AGAGAAGAAA 
     4201 ATGAAAAAAG AAGTCATCAT AAAGACCACT CAGATAGTGA ATCTACATCG TCAGATAATT 
     4261 CTGGGAGGAG GAGGAAAGGA CCCTTTAAAA CCATAAAGTT TGGGACCAAT ATTGACCTAT 
     4321 CTGATGACAA AAAGTGGAAG TTGCAGCTAC ATGAGCTGAC TAAACTTCCT GCTTTTGTGC 
     4381 GTGTCGTATC AGCAGGAAAT CTTCTAAGCC ATGTTGGTCA TACCATATTG GGCATGAACA 
     4441 CAGTTCAACT ATACATGAAA GTTCCAGGGA GCAGAACACC AGGTCATCAG GAAAATAACA 
     4501 ACTTCTGTTC AGTTAACATA AATATTGGCC CAGGTGACTG TGAATGGTTT GTTGTTCCTG 
     4561 AAGGTTACTG GGGTGTTCTG AATGACTTCT GTGAAAAAAA TAATTTGAAT TTCCTAATGG 
     4621 GTTCTTGGTG GCCCAATCTT GAAGATCTTT ATGAAGCAAA TGTTCCAGTG TATAGGTTTA 
     4681 TTCAGCGACC TGGAGATTTG GTCTGGATAA ATGCAGGCAC TGTTCATTGG GTTCAGGCTA 
     4741 TTGGCTGGTG CAACAACATT GCTTGGAATG TTGGTCCACT TACAGCCTGC CAGTATAAAT 
     4801 TGGCAGTGGA ACGGTACGAA TGGAACAAAT TGCAAAGTGT GAAGTCAATA GTACCCATGG 
     4861 TTCATCTTTC CTGGAATATG GCACGAAATA TCAAGGTCTC AGATCCAAAG CTTTTTGAAA 
     4921 TGATTAAGTA TTGTCTTCTA AGAACTCTGA AGCAATGTCA GACATTGAGG GAAGCTCTCA 
     4981 TTGCTGCAGG AAAAGAGATT ATATGGCATG GGCGGACAAA AGAAGAACCA GCTCATTACT 
     5041 GTAGCATTTG TGAAGTGGAG GTTTTTGATC TGCTTTTTGT CACTAATGAG AGTAATTCAC 
     5101 GAAAGACCTA CATAGTACAT TGCCAAGATT GTGCACGAAA AACAAGCGGA AACTTGGAAA 
     5161 ACTTTGTGGT GCTAGAACAG TACAAAATGG AGGACCTGAT GCAAGTCTAT GACCAATTTA 
     5221 CATTAGCTCC TCCATTACCA TCCGCCTCAT CTACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     5281 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     5341 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     5401 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5461 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5521 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5581 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5641 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5701 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5761 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5821 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5881 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5941 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     6001 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     6061 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     6121 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     6181 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     6241 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     6301 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     6361 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6421 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6481 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6541 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6601 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6661 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6721 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6781 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6841 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6901 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6961 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     7021 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     7081 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     7141 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     7201 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     7261 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     7321 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     7381 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7441 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7501 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7561 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7621 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7681 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7741 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7801 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7861 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7921 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7981 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     8041 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     8101 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     8161 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     8221 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     8281 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     8341 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8401 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8461 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8521 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8581 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8641 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8701 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8761 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8821 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8881 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8941 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     9001 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     9061 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//