LOCUS       RC240129                9036 bp    DNA     circular  SYN 
DEFINITION  RICTOR (myc-DDK-tagged) - Human RPTOR independent companion of MTOR, complex 2 (RICTOR), transcript variant 3
ACCESSION   NM_001285440
VERSION     NM_001285440.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5193..5284 
                     /label="Myc-DDK"  
     misc_feature    5314..5899 
                     /label="PolyA signal"  
     misc_feature    5371..5390 
                     /label="Restriction Enzyme"  
     misc_feature    6175..6847 
                     /label="ColE1"  
     misc_feature    7277..8071 
                     /label="Kan/Neo"  
     misc_feature    8106..8408 
                     /label="SV40 ori"  
     misc_feature    8494..8971 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5175..5181 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5175 
                     /label="RICTOR"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGAATCATA GCAACATTCC GATCATGGGC AGGTATTATT AATTTATGTA 
     1081 AACCTGGAAA TTCTGGGATC CAGTCTCTAA TAGGAGTACT TTGCATACCA AATATGGAAA 
     1141 TAAGGCGAGG TCTACTTGAA GTGCTTTATG ATATATTTCG TCTTCCTCTA CCTGTTGTGA 
     1201 CTGAGGAGTT CATAGAAGCA CTACTCAGTG TAGATCCAGG GAGGTTCCAA GACAGTTGGA 
     1261 GGCTTTCAGA TGGCTTTGTG GCAGCTGAGG CAAAAACTAT TCTTCCTCAT CGTGCCAGAT 
     1321 CCAGGCCAGA CCTCATGGAT AATTATTTGG CACTGATACT CTCTGCATTT ATTCGTAATG 
     1381 GACTTTTAGA GGGTCTAGTT GAAGTGATAA CAAACAGTGA TGATCATATC TCAGTTAGAG 
     1441 CTACCATCCT TTTAGGAGAG CTTTTACATA TGGCAAACAC AATTCTTCCT CATTCACATA 
     1501 GCCATCATTT ACACTGCTTG CCAACCCTAA TGAATATGGC TGCATCCTTT GATATCCCCA 
     1561 AGGAAAAGAG ACTGCGAGCC AGTGCAGCCT TGAACTGTTT AAAACGCTTC CATGAAATGA 
     1621 AGAAACGAGG ACCTAAGCCT TATAGTCTTC ATTTAGACCA CATTATTCAG AAAGCAATTG 
     1681 CAACACACCA GAAACGGGAT CAGTATCTCC GAGTTCAGAA AGATATATTT ATCCTTAAGG 
     1741 ATACAGAGGA AGCTCTTTTA ATTAACCTTA GAGATAGCCA AGTCCTTCAA CATAAAGAGA 
     1801 ATCTTGAATG GAATTGGAAT CTTATAGGGA CCATTCTTAA GTGGCCAAAT GTAAATCTAA 
     1861 GAAACTATAA AGATGAACAG TTACACAGGT TTGTACGAAG ACTACTTTAT TTTTACAAGC 
     1921 CCAGCAGTAA ATTATATGCC AACCTGGATC TGGATTTTGC CAAGGCCAAA CAGCTCACGG 
     1981 TTGTAGGTTG CCAGTTTACA GAATTTCTTC TTGAATCTGA AGAGGATGGG CAAGGCTACT 
     2041 TAGAAGATCT AGTAAAGGAT ATTGTTCAGT GGCTCAATGC TTCATCTGGA ATGAAACCCG 
     2101 AAAGAAGTCT TCAAAATAAT GGTTTATTGA CCACCCTTAG TCAACACTAC TTTTTATTTA 
     2161 TTGGAACACT TTCTTGCCAC CCTCATGGAG TTAAAATGCT GGAAAAATGC AGTGTATTTC 
     2221 AGTGTCTCCT TAATCTTTGC TCCTTGAAAA ACCAAGATCA CTTGCTAAAA CTTACTGTTT 
     2281 CTAGCTTGGA CTATAGCAGA GATGGATTGG CTAGAGTCAT CCTTTCCAAA ATTTTAACTG 
     2341 CAGCTACTGA TGCCTGCAGA CTCTATGCAA CAAAACATTT AAGGGTATTA TTGAGAGCTA 
     2401 ATGTTGAATT CTTTAATAAT TGGGGAATTG AGTTGTTAGT GACCCAGCTA CATGATAAAA 
     2461 ACAAAACGAT TTCCTCTGAA GCTCTTGATA TCCTCGATGA AGCATGTGAA GACAAGGCCA 
     2521 ATCTTCATGC TCTCATTCAG ATGAAACCAG CGTTATCCCA CCTTGGAGAC AAGGGTTTGC 
     2581 TTCTCCTGCT GAGATTTCTC TCCATTCCAA AAGGATTTTC CTATCTGAAT GAAAGAGGTT 
     2641 ATGTAGCAAA ACAATTGGAA AAGTGGCACA GGGAATACAA CTCCAAATAT GTTGACTTGA 
     2701 TTGAGGAACA ACTCAATGAA GCACTTACTA CTTACCGGAA GCCTGTTGAT GGTGATAACT 
     2761 ATGTTCGTCG GAGTAACCAA AGATTACAGC GTCCTCACGT CTACCTGCCT ATACACCTTT 
     2821 ATGGACAACT AGTACACCAT AAAACAGGCT GCCATTTGTT GGAAGTACAG AATATTATTA 
     2881 CAGAACTCTG TCGTAATGTT CGTACACCAG ATTTGGATAA GTGGGAAGAA ATTAAAAAAC 
     2941 TGAAAGCATC TCTTTGGGCC TTGGGAAATA TCGGCTCATC AAATTGGGGT CTCAATTTGC 
     3001 TACAGGAAGA AAACGTGATT CCAGATATAC TAAAACTTGC AAAACAGTGT GAAGTTCTTT 
     3061 CCATCAGAGG GACCTGTGTA TATGTACTTG GGCTCATAGC TAAAACCAAA CAAGGCTGTG 
     3121 ATATTCTAAA ATGTCACAAC TGGGATGCTG TGAGGCATAG TCGCAAACAT CTGTGGCCAG 
     3181 TGGTTCCAGA TGATGTGGAA CAACTCTGTA ATGAACTTTC ATCTATCCCA AGCACTCTAA 
     3241 GTTTGAACTC GGAGTCAACC AGCTCTAGAC ATAATAGTGA AAGTGAATCT GTGCCATCGA 
     3301 GTATGTTCAT ATTGGAGGAT GACCGGTTTG GCAGCAGCTC TACTAGTACA TTTTTCCTTG 
     3361 ATATCAATGA AGATACAGAG CCAACATTTT ATGACCGATC TGGACCCATA AAGGATAAAA 
     3421 ATTCATTCCC TTTCTTTGCT TCTAGTAAAC TTGTGAAGAA TCGTATCTTA AATTCGCTTA 
     3481 CTTTGCCTAA CAAAAAACAT CGTAGTAGCA GTGATCCAAA AGGAGGGAAA TTATCATCTG 
     3541 AAAGTAAGAC AAGCAACAGG CGAATCAGAA CACTTACGGA GCCCAGTGTT GATTTTAATC 
     3601 ATAGTGATGA TTTTACACCC ATATCCACTG TACAGAAAAC ATTACAATTA GAGACTTCAT 
     3661 TTATGGGGAA TAAGCACATT GAAGACACTG GTAGTACACC AAGCATTGGA GAAAATGACT 
     3721 TAAAATTCAC CAAGAATTTT GGTACAGAGA ATCACAGAGA AAATACAAGC CGAGAGAGGT 
     3781 TAGTAGTAGA AAGTTCAACG AGCTCACATA TGAAGATACG TAGCCAAAGT TTCAATACAG 
     3841 ACACTACAAC AAGTGGCATA AGTTCAATGA GCTCAAGTCC TTCACGAGAG ACAGTAGGTG 
     3901 TAGATGCTAC AACTATGGAC ACAGACTGTG GAAGCATGAG TACTGTGGTA AGTACTAAAA 
     3961 CTATTAAGAC AAGCCACTAT TTGACGCCAC AGTCTAACCA TCTGTCTCTC TCCAAATCAA 
     4021 ATTCGGTGTC CCTGGTGCCT CCAGGTTCTT CTCATACGCT TCCTAGAAGA GCACAGTCCC 
     4081 TTAAAGCACC CTCTATTGCT ACAATTAAAA GTCTAGCAGA TTGTAACTTT AGTTACACAA 
     4141 GTTCTAGAGA TGCTTTTGGC TATGCTACAC TGAAAAGACT ACAGCAACAA AGAATGCATC 
     4201 CATCCTTATC TCACTCTGAA GCTTTGGCAT CTCCAGCAAA AGATGTGCTA TTTACTGATA 
     4261 CCATCACCAT GAAGGCCAAC AGTTTTGAGT CCAGATTAAC ACCAAGCAGG TTCATGAAAG 
     4321 CCTTAAGTTA TGCATCATTA GATAAAGAAG ATTTATTGAG TCCTATTAAT CAAAATACCC 
     4381 TGCAACGATC TTCCTCAGTG CGGTCCATGG TGTCCAGTGC CACATATGGG GGTTCAGATG 
     4441 ATTACATTGG TCTTGCTCTC CCGGTGGATA TAAATGATAT ATTCCAGGTA AAGGATATTC 
     4501 CCTATTTTCA GACAAAAAAC ATACCACCAC ATGATGATCG AGGTGCAAGA GCATTTGCCC 
     4561 ATGATGCAGG AGAAAGTACA GAAGACACTG GACTACAGGA ACATACAGAT GATAACTGCC 
     4621 TTTATTGTGT CTGTATTGAA ATTCTGGGTT TCCAGCCCAG CAACCAACTG AGTGCAATAT 
     4681 GTAGTCATTC AGACTTTCAA GATATTCCAT ATTCTGATTG GTGTGAGCAG ACTATCCATA 
     4741 ATCCTTTAGA AGTGGTTCCC TCTAAGTTTT CGGGGATTTC TGGATGCAGT GATGGGGTGT 
     4801 CTCAAGAAGG CTCAGCTAGC AGCACCAAAA GCACAGAATT GTTACTAGGT GTTAAAACAA 
     4861 TTCCAGATGA TACACCAATG TGCCGTATAC TCCTTCGCAA AGAAGTTCTA AGATTAGTCA 
     4921 TTAATTTGAG TAGTTCAGTT TCAACTAAAT GTCATGAGAC TGGGCTTTTA ACAATTAAGG 
     4981 AGAAGTATCC TCAAACATTT GATGACATAT GCCTTTACTC TGAGGTTTCC CATTTGCTGT 
     5041 CACACTGCAC ATTCAGACTT CCGTGTCGGA GGTTCATACA AGAATTATTT CAAGATGTAC 
     5101 AGTTTCTACA AATGCATGAA GAAGCAGAGG CTGTGTTGGC AACACCACCA AAGCAACCTA 
     5161 TAGTTGATAC ATCTGCTGAA TCCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     5221 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     5281 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     5341 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     5401 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     5461 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5521 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5581 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5641 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5701 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5761 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5821 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5881 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5941 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     6001 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     6061 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     6121 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     6181 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     6241 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     6301 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     6361 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     6421 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6481 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6541 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6601 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6661 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6721 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6781 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6841 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6901 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6961 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     7021 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     7081 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     7141 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     7201 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     7261 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     7321 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     7381 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     7441 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7501 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7561 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7621 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7681 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7741 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7801 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7861 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7921 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7981 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     8041 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     8101 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     8161 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     8221 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     8281 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8341 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8401 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     8461 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8521 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8581 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8641 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8701 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8761 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8821 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8881 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8941 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     9001 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//