LOCUS       RC240128                9027 bp    DNA     circular  SYN 
DEFINITION  ROBO2 (myc-DDK-tagged) - Human roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant 3
ACCESSION   NM_001290039
VERSION     NM_001290039.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5184..5275 
                     /label="Myc-DDK"  
     misc_feature    5305..5890 
                     /label="PolyA signal"  
     misc_feature    5362..5381 
                     /label="Restriction Enzyme"  
     misc_feature    6166..6838 
                     /label="ColE1"  
     misc_feature    7268..8062 
                     /label="Kan/Neo"  
     misc_feature    8097..8399 
                     /label="SV40 ori"  
     misc_feature    8485..8962 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5166..5172 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5166 
                     /label="ROBO2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTCTGCTG ATGTTTACAC AACTACTGCT CTGTGGATTT TTATATGTTC 
     1081 GGGTTGATGG ATCGCGTCTT CGCCAGGAGG ACTTTCCCCC GCGGATTGTG GAGCATCCTT 
     1141 CCGATGTCAT CGTCTCTAAG GGCGAGCCCA CGACTCTGAA CTGCAAGGCG GAGGGCCGGC 
     1201 CAACGCCCAC CATTGAGTGG TACAAAGATG GGGAGCGAGT GGAGACTGAC AAGGACGATC 
     1261 CCCGGTCCCA CAGGATGCTT CTGCCCAGCG GATCCTTATT CTTCTTGCGC ATCGTGCACG 
     1321 GGCGCAGGAG TAAACCTGAT GAAGGAAGCT ACGTTTGTGT TGCGAGGAAC TATCTTGGTG 
     1381 AAGCAGTGAG TCGAAATGCG TCTCTGGAAG TGGCATTGTT ACGAGATGAC TTCCGACAAA 
     1441 ACCCCACAGA TGTTGTAGTG GCAGCTGGAG AGCCTGCAAT CCTGGAGTGC CAGCCTCCCC 
     1501 GGGGACACCC AGAACCCACC ATCTACTGGA AAAAAGACAA AGTTCGAATT GATGACAAGG 
     1561 AAGAAAGAAT AAGTATCCGT GGTGGAAAAC TGATGATCTC CAATACCAGG AAAAGTGATG 
     1621 CAGGGATGTA TACTTGTGTT GGTACCAATA TGGTGGGAGA AAGGGACAGT GACCCAGCAG 
     1681 AGCTGACTGT CTTTGAACGA CCCACATTTC TCAGGAGGCC AATTAACCAG GTGGTACTGG 
     1741 AGGAAGAAGC TGTAGAATTT CGTTGTCAAG TCCAAGGAGA TCCTCAACCA ACTGTGAGGT 
     1801 GGAAAAAGGA TGATGCAGAC TTGCCAAGAG GAAGGTATGA CATCAAAGAC GATTACACAC 
     1861 TAAGAATTAA AAAGACCATG AGTACAGATG AAGGCACCTA TATGTGTATT GCTGAGAATC 
     1921 GGGTTGGAAA AATGGAAGCC TCTGCTACAC TCACCGTCCG AGCTCGCCCT GTTGCTCCCC 
     1981 CACAGTTTGT GGTTCGGCCA AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC 
     2041 CCTGTGAAAC TAAAGGAAAC CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA 
     2101 ACCTACTTTT CCCAAACCAA CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG 
     2161 GAGACCTCAC AATCACCAAC ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT 
     2221 TAACTGTGGC AGGAAGCATT TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG 
     2281 ATAGACCTCC ACCTATAATT CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA 
     2341 CAGCGTTACT GAAATGTAAA GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG 
     2401 AGGGATTTAC TTTTCCGGGT AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC 
     2461 AGATTAAGAA TTTACGGATT TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA 
     2521 GTGGAGAGAC TTCCTGGAGT GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA 
     2581 AAAACTATGA TTTAAGTGAC CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA 
     2641 CTAAGAACAG TGTCACCTTG TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG 
     2701 CATATATCAT TGAGGCTTTC AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC 
     2761 ATGTAAAGAC CACCCTCTAT ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA 
     2821 TGGTCAGAGC GATCAACCCC CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC 
     2881 GCACACAAGA TATCAGCCCA CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC 
     2941 TAGGAGATGT CCTTGTCCGT CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG 
     3001 TCACATGGAC GGTTGATCGC CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC 
     3061 AGACTTCAGG TCTGCAGGCG ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG 
     3121 AACGAAGTGC TGTCTTAGTC AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC 
     3181 CATATTTTAA TGAGTTCCAA GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG 
     3241 AAGCCCCAAG TGCCCCACCA CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA 
     3301 CAAGTATTAG TGTTTCCTGG GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG 
     3361 AATACAAGAT CTGGTGTCTA GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG 
     3421 CAGCCATTCG GTCCGTAATA ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG 
     3481 TTGCAGCTAG TACCAGTGCA GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG 
     3541 GGAGACGCAA TGAAGTTGTC ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG 
     3601 ATGTGGTGAA GCAACCAGCC TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA 
     3661 TGGGTTTTAG CATATGGTTG TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG 
     3721 CTGTTACGTT TCAAAGAGGA GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC 
     3781 TCAATGCTGG TGATCCCAGC TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC 
     3841 CAGTAAATAA TAGCAACAGT GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC 
     3901 TGCCACCAGT TCCAGGCCAA GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT 
     3961 ATAGTAGCAT TGACTTCACT ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG 
     4021 CTACCCCATA TGCCACGACA CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG 
     4081 ATCTGCCTGA TCCACAATGG AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG 
     4141 GTTATTCTCT ACCTGATCAG AACAAAGGTA ACAATGGTGG GAAAGGTGGA AAAAAGAAGA 
     4201 AAAATAAAAA CTCTTCTAAA CCACAGAAAA ACAATGGATC CACTTGGGCC AATGTCCCTC 
     4261 TACCTCCCCC CCCAGTCCAG CCCCTTCCTG GCACGGAGCT GGAACACTAT GCAGTGGAAC 
     4321 AACAAGAAAA TGGCTATGAC AGTGATAGCT GGTGCCCACC ATTGCCAGTA CAAACTTACT 
     4381 TACACCAAGG TCTGGAAGAT GAACTGGAAG AAGATGATGA TAGGGTCCCA ACACCTCCTG 
     4441 TTCGAGGCGT GGCTTCTTCT CCTGCTATCT CCTTTGGACA GCAGTCCACT GCAACTCTTA 
     4501 CTCCATCCCC ACGGGAAGAG ATGCAACCCA TGCTGCAGGC TCACCTGGAT GAGTTGACAA 
     4561 GAGCCTATCA GTTTGATATA GCAAAACAAA CATGGCACAT TCAAAGCAAT AATCAACCTC 
     4621 CACAGCCTCC AGTTCCACCG TTAGGTTATG TGTCTGGAGC CTTGATTTCT GATTTGGAAA 
     4681 CGGATGTTGC AGATGATGAT GCCGACGACG AAGAGGAAGC TTTAGAAATC CCCAGGCCCC 
     4741 TGAGAGCACT GGACCAGACT CCTGGATCCA GCATGGACAA TCTAGACAGC TCTGTGACAG 
     4801 GAAAAGCCTT TACCTCCTCT CAAAGACCTC GACCTACCAG CCCATTTTCT ACTGACAGTA 
     4861 ACACCAGTGC AGCCCTGAGT CAAAGTCAGA GGCCTCGGCC CACTAAAAAA CACAAGGGAG 
     4921 GGCGGATGGA CCAACAACCA GCATTGCCTC ATCGAAGGGA AGGAATGACA GATGAGGAGG 
     4981 CCTTGGTGCC CTATAGCAAG CCCAGTTTCC CATCTCCAGG TGGCCACAGC TCATCAGGAA 
     5041 CAGCTTCTTC TAAGGGATCC ACTGGACCTA GGAAAACCGA GGTGTTGAGA GCAGGCCACC 
     5101 AGCGCAATGC CAGCGACCTT CTTGACATAG GATATATGGG CTCCAACAGT CAAGGACAGT 
     5161 TTACAGGTGA ATTAACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     5221 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     5281 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     5341 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     5401 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     5461 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5521 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5581 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5641 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5701 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5761 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5821 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5881 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5941 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     6001 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     6061 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     6121 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     6181 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     6241 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     6301 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     6361 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     6421 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6481 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6541 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6601 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6661 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6721 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6781 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6841 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6901 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6961 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     7021 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     7081 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     7141 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     7201 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     7261 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     7321 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     7381 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     7441 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7501 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7561 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7621 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7681 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7741 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7801 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7861 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7921 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7981 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     8041 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     8101 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     8161 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     8221 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     8281 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8341 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8401 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     8461 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8521 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8581 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8641 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8701 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8761 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8821 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8881 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8941 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     9001 ATTTAACAAA AATTTAACGC GAATTTT
//