LOCUS       RC240126                8979 bp    DNA     circular  SYN 
DEFINITION  IGF1R (myc-DDK-tagged) - Human insulin-like growth factor 1 receptor (IGF1R), transcript variant 2
ACCESSION   NM_001291858
VERSION     NM_001291858.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5136..5227 
                     /label="Myc-DDK"  
     misc_feature    5257..5842 
                     /label="PolyA signal"  
     misc_feature    5314..5333 
                     /label="Restriction Enzyme"  
     misc_feature    6118..6790 
                     /label="ColE1"  
     misc_feature    7220..8014 
                     /label="Kan/Neo"  
     misc_feature    8049..8351 
                     /label="SV40 ori"  
     misc_feature    8437..8914 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5118..5124 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5118 
                     /label="IGF1R"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGTCTGGC TCCGGAGGAG GGTCCCCGAC CTCGCTGTGG GGGCTCCTGT 
     1081 TTCTCTCCGC CGCGCTCTCG CTCTGGCCGA CGAGTGGAGA AATCTGCGGG CCAGGCATCG 
     1141 ACATCCGCAA CGACTATCAG CAGCTGAAGC GCCTGGAGAA CTGCACGGTG ATCGAGGGCT 
     1201 ACCTCCACAT CCTGCTCATC TCCAAGGCCG AGGACTACCG CAGCTACCGC TTCCCCAAGC 
     1261 TCACGGTCAT TACCGAGTAC TTGCTGCTGT TCCGAGTGGC TGGCCTCGAG AGCCTCGGAG 
     1321 ACCTCTTCCC CAACCTCACG GTCATCCGCG GCTGGAAACT CTTCTACAAC TACGCCCTGG 
     1381 TCATCTTCGA GATGACCAAT CTCAAGGATA TTGGGCTTTA CAACCTGAGG AACATTACTC 
     1441 GGGGGGCCAT CAGGATTGAG AAAAATGCTG ACCTCTGTTA CCTCTCCACT GTGGACTGGT 
     1501 CCCTGATCCT GGATGCGGTG TCCAATAACT ACATTGTGGG GAATAAGCCC CCAAAGGAAT 
     1561 GTGGGGACCT GTGTCCAGGG ACCATGGAGG AGAAGCCGAT GTGTGAGAAG ACCACCATCA 
     1621 ACAATGAGTA CAACTACCGC TGCTGGACCA CAAACCGCTG CCAGAAAATG TGCCCAAGCA 
     1681 CGTGTGGGAA GCGGGCGTGC ACCGAGAACA ATGAGTGCTG CCACCCCGAG TGCCTGGGCA 
     1741 GCTGCAGCGC GCCTGACAAC GACACGGCCT GTGTAGCTTG CCGCCACTAC TACTATGCCG 
     1801 GTGTCTGTGT GCCTGCCTGC CCGCCCAACA CCTACAGGTT TGAGGGCTGG CGCTGTGTGG 
     1861 ACCGTGACTT CTGCGCCAAC ATCCTCAGCG CCGAGAGCAG CGACTCCGAG GGGTTTGTGA 
     1921 TCCACGACGG CGAGTGCATG CAGGAGTGCC CCTCGGGCTT CATCCGCAAC GGCAGCCAGA 
     1981 GCATGTACTG CATCCCTTGT GAAGGTCCTT GCCCGAAGGT CTGTGAGGAA GAAAAGAAAA 
     2041 CAAAGACCAT TGATTCTGTT ACTTCTGCTC AGATGCTCCA AGGATGCACC ATCTTCAAGG 
     2101 GCAATTTGCT CATTAACATC CGACGGGGGA ATAACATTGC TTCAGAGCTG GAGAACTTCA 
     2161 TGGGGCTCAT CGAGGTGGTG ACGGGCTACG TGAAGATCCG CCATTCTCAT GCCTTGGTCT 
     2221 CCTTGTCCTT CCTAAAAAAC CTTCGCCTCA TCCTAGGAGA GGAGCAGCTA GAAGGGAATT 
     2281 ACTCCTTCTA CGTCCTCGAC AACCAGAACT TGCAGCAACT GTGGGACTGG GACCACCGCA 
     2341 ACCTGACCAT CAAAGCAGGG AAAATGTACT TTGCTTTCAA TCCCAAATTA TGTGTTTCCG 
     2401 AAATTTACCG CATGGAGGAA GTGACGGGGA CTAAAGGGCG CCAAAGCAAA GGGGACATAA 
     2461 ACACCAGGAA CAACGGGGAG AGAGCCTCCT GTGAAAGTGA CGTCCTGCAT TTCACCTCCA 
     2521 CCACCACGTC GAAGAATCGC ATCATCATAA CCTGGCACCG GTACCGGCCC CCTGACTACA 
     2581 GGGATCTCAT CAGCTTCACC GTTTACTACA AGGAAGCACC CTTTAAGAAT GTCACAGAGT 
     2641 ATGATGGGCA GGATGCCTGC GGCTCCAACA GCTGGAACAT GGTGGACGTG GACCTCCCGC 
     2701 CCAACAAGGA CGTGGAGCCC GGCATCTTAC TACATGGGCT GAAGCCCTGG ACTCAGTACG 
     2761 CCGTTTACGT CAAGGCTGTG ACCCTCACCA TGGTGGAGAA CGACCATATC CGTGGGGCCA 
     2821 AGAGTGAGAT CTTGTACATT CGCACCAATG CTTCAGTTCC TTCCATTCCC TTGGACGTTC 
     2881 TTTCAGCATC GAACTCCTCT TCTCAGTTAA TCGTGAAGTG GAACCCTCCC TCTCTGCCCA 
     2941 ACGGCAACCT GAGTTACTAC ATTGTGCGCT GGCAGCGGCA GCCTCAGGAC GGCTACCTTT 
     3001 ACCGGCACAA TTACTGCTCC AAAGACAAAA TCCCCATCAG GAAGTATGCC GACGGCACCA 
     3061 TCGACATTGA GGAGGTCACA GAGAACCCCA AGACTGAGGT GTGTGGTGGG GAGAAAGGGC 
     3121 CTTGCTGCGC CTGCCCCAAA ACTGAAGCCG AGAAGCAGGC CGAGAAGGAG GAGGCTGAAT 
     3181 ACCGCAAAGT CTTTGAGAAT TTCCTGCACA ACTCCATCTT CGTGCCCAGA CCTGAAAGGA 
     3241 AGCGGAGAGA TGTCATGCAA GTGGCCAACA CCACCATGTC CAGCCGAAGC AGGAACACCA 
     3301 CGGCCGCAGA CACCTACAAC ATCACCGACC CGGAAGAGCT GGAGACAGAG TACCCTTTCT 
     3361 TTGAGAGCAG AGTGGATAAC AAGGAGAGAA CTGTCATTTC TAACCTTCGG CCTTTCACAT 
     3421 TGTACCGCAT CGATATCCAC AGCTGCAACC ACGAGGCTGA GAAGCTGGGC TGCAGCGCCT 
     3481 CCAACTTCGT CTTTGCAAGG ACTATGCCCG CAGAAGGAGC AGATGACATT CCTGGGCCAG 
     3541 TGACCTGGGA GCCAAGGCCT GAAAACTCCA TCTTTTTAAA GTGGCCGGAA CCTGAGAATC 
     3601 CCAATGGATT GATTCTAATG TATGAAATAA AATACGGATC ACAAGTTGAG GATCAGCGAG 
     3661 AATGTGTGTC CAGACAGGAA TACAGGAAGT ATGGAGGGGC CAAGCTAAAC CGGCTAAACC 
     3721 CGGGGAACTA CACAGCCCGG ATTCAGGCCA CATCTCTCTC TGGGAATGGG TCGTGGACAG 
     3781 ATCCTGTGTT CTTCTATGTC CAGGCCAAAA GATATGAAAA CTTCATCCAT CTGATCATCG 
     3841 CTCTGCCCGT CGCTGTCCTG TTGATCGTGG GAGGGTTGGT GATTATGCTG TACGTCTTCC 
     3901 ATAGAAAGAG AAATAACAGC AGGCTGGGGA ATGGAGTGCT GTATGCCTCT GTGAACCCGG 
     3961 AGTACTTCAG CGCTGCTGAT GTGTACGTTC CTGATGAGTG GGAGGTGGCT CGGGAGAAGA 
     4021 TCACCATGAG CCGGGAACTT GGGCAGGGGT CGTTTGGGAT GGTCTATGAA GGAGTTGCCA 
     4081 AGGGTGTGGT GAAAGATGAA CCTGAAACCA GAGTGGCCAT TAAAACAGTG AACGAGGCCG 
     4141 CAAGCATGCG TGAGAGGATT GAGTTTCTCA ACGAAGCTTC TGTGATGAAG GAGTTCAATT 
     4201 GTCACCATGT GGTGCGATTG CTGGGTGTGG TGTCCCAAGG CCAGCCAACA CTGGTCATCA 
     4261 TGGAACTGAT GACACGGGGC GATCTCAAAA GTTATCTCCG GTCTCTGAGG CCAGAAATGG 
     4321 AGAATAATCC AGTCCTAGCA CCTCCAAGCC TGAGCAAGAT GATTCAGATG GCCGGAGAGA 
     4381 TTGCAGACGG CATGGCATAC CTCAACGCCA ATAAGTTCGT CCACAGAGAC CTTGCTGCCC 
     4441 GGAATTGCAT GGTAGCCGAA GATTTCACAG TCAAAATCGG AGATTTTGGT ATGACGCGAG 
     4501 ATATCTATGA GACAGACTAT TACCGGAAAG GAGGGAAAGG GCTGCTGCCC GTGCGCTGGA 
     4561 TGTCTCCTGA GTCCCTCAAG GATGGAGTCT TCACCACTTA CTCGGACGTC TGGTCCTTCG 
     4621 GGGTCGTCCT CTGGGAGATC GCCACACTGG CCGAGCAGCC CTACCAGGGC TTGTCCAACG 
     4681 AGCAAGTCCT TCGCTTCGTC ATGGAGGGCG GCCTTCTGGA CAAGCCAGAC AACTGTCCTG 
     4741 ACATGCTGTT TGAACTGATG CGCATGTGCT GGCAGTATAA CCCCAAGATG AGGCCTTCCT 
     4801 TCCTGGAGAT CATCAGCAGC ATCAAAGAGG AGATGGAGCC TGGCTTCCGG GAGGTCTCCT 
     4861 TCTACTACAG CGAGGAGAAC AAGCTGCCCG AGCCGGAGGA GCTGGACCTG GAGCCAGAGA 
     4921 ACATGGAGAG CGTCCCCCTG GACCCCTCGG CCTCCTCGTC CTCCCTGCCA CTGCCCGACA 
     4981 GACACTCAGG ACACAAGGCC GAGAACGGCC CCGGCCCTGG GGTGCTGGTC CTCCGCGCCA 
     5041 GCTTCGACGA GAGACAGCCT TACGCCCACA TGAACGGGGG CCGCAAGAAC GAGCGGGCCT 
     5101 TGCCGCTGCC CCAGTCTTCG ACCTGCACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     5161 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     5221 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     5281 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     5341 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     5401 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     5461 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     5521 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5581 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5641 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5701 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5761 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5821 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5881 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5941 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     6001 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     6061 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     6121 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     6181 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     6241 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     6301 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     6361 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     6421 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     6481 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6541 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6601 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6661 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6721 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6781 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6841 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6901 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6961 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     7021 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     7081 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     7141 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     7201 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     7261 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     7321 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     7381 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     7441 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     7501 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7561 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7621 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7681 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7741 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7801 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7861 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7921 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7981 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     8041 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     8101 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     8161 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     8221 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8281 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8341 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     8401 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     8461 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     8521 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     8581 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8641 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8701 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8761 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8821 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8881 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8941 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//