LOCUS       RC240125                8964 bp    DNA     circular  SYN 
DEFINITION  PRG4 (myc-DDK-tagged) - Human proteoglycan 4 (PRG4), transcript variant E
ACCESSION   NM_001303232
VERSION     NM_001303232.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5121..5212 
                     /label="Myc-DDK"  
     misc_feature    5242..5827 
                     /label="PolyA signal"  
     misc_feature    5299..5318 
                     /label="Restriction Enzyme"  
     misc_feature    6103..6775 
                     /label="ColE1"  
     misc_feature    7205..7999 
                     /label="Kan/Neo"  
     misc_feature    8034..8336 
                     /label="SV40 ori"  
     misc_feature    8422..8899 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5103..5109 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5103 
                     /label="PRG4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCATGGAAA ACACTTCCCA TTTACCTGTT GTTGCTGCTG TCTGTTTTCG 
     1081 TGATTCAGCA AGTTTCATCT CAAGATTTAT CAAGCTGTGC AGGGAGATGT GGGGAAGGGT 
     1141 ATTCTAGAGA TGCCACCTGC AACTGTGATT ATAACTGTCA ACACTACATG GAGTGCTGCC 
     1201 CTGATTTCAA GAGAGTCTGC ACTGCGGAGC TTTCCTGTAA AGGCCGCTGC TTTGAGTCCT 
     1261 TCGAGAGAGG GAGGGAGTGT GACTGCGACG CCCAATGTAA GAAGTATGAC AAGTGCTGTC 
     1321 CCGATTATGA GAGTTTCTGT GCAGAAGTGC ATAATCCCAC ATCACCACCA TCTTCAAAGA 
     1381 AAGCACCTCC ACCTTCAGGA GCATCTCAAA CCATCAAATC AACAACCAAA CGTTCACCCA 
     1441 AACCACCAAA CAAGAAGAAG ACTAAGAAAG TTATAGAATC AGAGGAAATA ACAGAAGTAA 
     1501 AAGATAACAA GAAGAACAGA ACTAAAAAGA AACCTACCCC CAAACCACCA GTTGTAGATG 
     1561 AAGCTGGAAG TGGATTGGAC AATGGTGACT TCAAGGTCAC AACTCCTGAC ACGTCTACCA 
     1621 CCCAACACAA TAAAGTCAGC ACATCTCCCA AGATCACAAC AGCAAAACCA ATAAATCCCA 
     1681 GACCCAGTCT TCCACCTAAT TCTGATACAT CTAAAGAGAC GTCTTTGACA GTGAATAAAG 
     1741 AGACAACAGT TGAAACTAAA GAAACTACTA CAACAAATAA ACAGACTTCA ACTGATGGAA 
     1801 AAGAGAAGAC TACTTCCGCT AAAGAGACAC AAAGTATAGA GAAAACATCT GCTAAAGATT 
     1861 TAGCACCCAC ATCTAAAGTG CTGGCTAAAC CTACACCCAA AGCTGAAACT ACAACCAAAG 
     1921 GCCCTGCTCT CACCACTCCC AAGGAGCCCA CGCCCACCAC TCCCAAGGAG CCTGCATCTA 
     1981 CCACACCCAA AGAGCCCACA CCTACCACCA TCAAGTCTGC ACCCACCACC CCCAAGGAGC 
     2041 CTGCACCCAC CACCACCAAG TCTGCACCCA CCACTCCCAA GGAGCCTGCA CCCACCACCA 
     2101 CCAAGGAGCC TGCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCACC AAGGAGCCTG 
     2161 CACCCACCAC CACCAAGTCT GCACCCACCA CTCCCAAGGA GCCTGCACCC ACCACCCCCA 
     2221 AGAAGCCTGC CCCAACTACC CCCAAGGAGC CTGCACCCAC CACTCCCAAG GAGCCTACAC 
     2281 CCACCACTCC CAAGGAGCCT GCACCCACCA CCAAGGAGCC TGCACCCACC ACTCCCAAAG 
     2341 AGCCTGCACC CACTGCCCCC AAGAAGCCTG CCCCAACTAC CCCCAAGGAG CCTGCACCCA 
     2401 CCACTCCCAA GGAGCCTGCA CCCACCACCA CCAAGGAGCC TTCACCCACC ACTCCCAAGG 
     2461 AGCCTGCACC CACCACCACC AAGTCTGCAC CCACCACTAC CAAGGAGCCT GCACCCACCA 
     2521 CTACCAAGTC TGCACCCACC ACTCCCAAGG AGCCTTCACC CACCACCACC AAGGAGCCTG 
     2581 CACCCACCAC TCCCAAGGAG CCTGCACCCA CCACCCCCAA GAAGCCTGCC CCAACTACCC 
     2641 CCAAGGAGCC TGCACCCACC ACTCCCAAGG AACCTGCACC CACCACCACC AAGAAGCCTG 
     2701 CACCCACCAC TCCCAAAGAG CCTGCCCCAA CTACCCCCAA GGAGACTGCA CCCACCACCC 
     2761 CCAAGAAGCT CACGCCCACC ACCCCCGAGA AGCTCGCACC CACCACCCCT GAGAAGCCCG 
     2821 CACCCACCAC CCCTGAGGAG CTCGCACCCA CCACCCCTGA GGAGCCCACA CCCACCACCC 
     2881 CTGAGGAGCC TGCTCCCACC ACTCCCAAGG CAGCGGCTCC CAACACCCCT AAGGAGCCTG 
     2941 CTCCAACTAC CCCTAAGGAG CCTGCTCCAA CTACCCCTAA GGAGCCTGCT CCAACTACCC 
     3001 CTAAGGAGAC TGCTCCAACT ACCCCTAAAG GGACTGCTCC AACTACCCTC AAGGAACCTG 
     3061 CACCCACTAC TCCCAAGAAG CCTGCCCCCA AGGAGCTTGC ACCCACCACC ACCAAGGAGC 
     3121 CCACATCCAC CACCTCTGAC AAGCCCGCTC CAACTACCCC TAAGGGGACT GCTCCAACTA 
     3181 CCCCTAAGGA GCCTGCTCCA ACTACCCCTA AGGAGCCTGC TCCAACTACC CCTAAGGGGA 
     3241 CTGCTCCAAC TACCCTCAAG GAACCTGCAC CCACTACTCC CAAGAAGCCT GCCCCCAAGG 
     3301 AGCTTGCACC CACCACCACC AAGGGGCCCA CATCCACCAC CTCTGACAAG CCTGCTCCAA 
     3361 CTACACCTAA GGAGACTGCT CCAACTACCC CCAAGGAGCC TGCACCCACT ACCCCCAAGA 
     3421 AGCCTGCTCC AACTACTCCT GAGACACCTC CTCCAACCAC TTCAGAGGTC TCTACTCCAA 
     3481 CTACCACCAA GGAGCCTACC ACTATCCACA AAAGCCCTGA TGAATCAACT CCTGAGCTTT 
     3541 CTGCAGAACC CACACCAAAA GCTCTTGAAA ACAGTCCCAA GGAACCTGGT GTACCTACAA 
     3601 CTAAGACTCC TGCAGCGACT AAACCTGAAA TGACTACAAC AGCTAAAGAC AAGACAACAG 
     3661 AAAGAGACTT ACGTACTACA CCTGAAACTA CAACTGCTGC ACCTAAGATG ACAAAAGAGA 
     3721 CAGCAACTAC AACAGAAAAA ACTACCGAAT CCAAAATAAC AGCTACAACC ACACAAGTAA 
     3781 CATCTACCAC AACTCAAGAT ACCACACCAT TCAAAATTAC TACTCTTAAA ACAACTACTC 
     3841 TTGCACCCAA AGTAACTACA ACAAAAAAGA CAATTACTAC CACTGAGATT ATGAACAAAC 
     3901 CTGAAGAAAC AGCTAAACCA AAAGACAGAG CTACTAATTC TAAAGCGACA ACTCCTAAAC 
     3961 CTCAAAAGCC AACCAAAGCA CCCAAAAAAC CCACTTCTAC CAAAAAGCCA AAAACAATGC 
     4021 CTAGAGTGAG AAAACCAAAG ACGACACCAA CTCCCCGCAA GATGACATCA ACAATGCCAG 
     4081 AATTGAACCC TACCTCAAGA ATAGCAGAAG CCATGCTCCA AACCACCACC AGACCTAACC 
     4141 AAACTCCAAA CTCCAAACTA GTTGAAGTAA ATCCAAAGAG TGAAGATGCA GGTGGTGCTG 
     4201 AAGGAGAAAC ACCTCATATG CTTCTCAGGC CCCATGTGTT CATGCCTGAA GTTACTCCCG 
     4261 ACATGGATTA CTTACCGAGA GTACCCAATC AAGGCATTAT CATCAATCCC ATGCTTTCCG 
     4321 ATGAGACCAA TATATGCAAT GGTAAGCCAG TAGATGGACT GACTACTTTG CGCAATGGGA 
     4381 CATTAGTTGC ATTCCGAGGT CATTATTTCT GGATGCTAAG TCCATTCAGT CCACCATCTC 
     4441 CAGCTCGCAG AATTACTGAA GTTTGGGGTA TTCCTTCCCC CATTGATACT GTTTTTACTA 
     4501 GGTGCAACTG TGAAGGAAAA ACTTTCTTCT TTAAGGATTC TCAGTACTGG CGTTTTACCA 
     4561 ATGATATAAA AGATGCAGGG TACCCCAAAC CAATTTTCAA AGGATTTGGA GGACTAACTG 
     4621 GACAAATAGT GGCAGCGCTT TCAACAGCTA AATATAAGAA CTGGCCTGAA TCTGTGTATT 
     4681 TTTTCAAGAG AGGTGGCAGC ATTCAGCAGT ATATTTATAA ACAGGAACCT GTACAGAAGT 
     4741 GCCCTGGAAG AAGGCCTGCT CTAAATTATC CAGTGTATGG AGAAACGACA CAGGTTAGGA 
     4801 GACGTCGCTT TGAACGTGCT ATAGGACCTT CTCAAACACA CACCATCAGA ATTCAATATT 
     4861 CACCTGCCAG ACTGGCTTAT CAAGACAAAG GTGTCCTTCA TAATGAAGTT AAAGTGAGTA 
     4921 TACTGTGGAG AGGACTTCCA AATGTGGTTA CCTCAGCTAT ATCACTGCCC AACATCAGAA 
     4981 AACCTGACGG CTATGATTAC TATGCCTTTT CTAAAGATCA ATACTATAAC ATTGATGTGC 
     5041 CTAGTAGAAC AGCAAGAGCA ATTACTACTC GTTCTGGGCA GACCTTATCC AAAGTCTGGT 
     5101 ACAACTGTCC TACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     5161 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     5221 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     5281 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     5341 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5401 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5461 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5521 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5581 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5641 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5701 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5761 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5821 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5881 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5941 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     6001 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     6061 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     6121 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     6181 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     6241 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     6301 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6361 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6421 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6481 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6541 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6601 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6661 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6721 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6781 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6841 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6901 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6961 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     7021 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     7081 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     7141 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     7201 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     7261 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     7321 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7381 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7441 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7501 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7561 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7621 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7681 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7741 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7801 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7861 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7921 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7981 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     8041 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     8101 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     8161 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     8221 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8281 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8341 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8401 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8461 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8521 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8581 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8641 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8701 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8761 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8821 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8881 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8941 TAACAAAAAT TTAACGCGAA TTTT
//