LOCUS       RC240122                8949 bp    DNA     circular  SYN 
DEFINITION  KDM6A (myc-DDK-tagged) - Human lysine (K)-specific demethylase 6A (KDM6A), transcript variant 4
ACCESSION   NM_001291417
VERSION     NM_001291417.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5106..5197 
                     /label="Myc-DDK"  
     misc_feature    5227..5812 
                     /label="PolyA signal"  
     misc_feature    5284..5303 
                     /label="Restriction Enzyme"  
     misc_feature    6088..6760 
                     /label="ColE1"  
     misc_feature    7190..7984 
                     /label="Kan/Neo"  
     misc_feature    8019..8321 
                     /label="SV40 ori"  
     misc_feature    8407..8884 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5088..5094 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5088 
                     /label="KDM6A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAATCCTGC GGAGTGTCGC TCGCTACCGC CGCCGCTGCC GCCGCCGCTT 
     1081 TCGGTGATGA GGAAAAGAAA ATGGCGGCGG GAAAAGCGAG CGGCGAGAGC GAGGAGGCGT 
     1141 CCCCCAGCCT GACAGCCGAG GAGAGGGAGG CGCTCGGCGG ACTGGACAGC CGCCTCTTTG 
     1201 GGTTCGTGAG ATTTCATGAA GATGGCGCCA GGACGAAGGC CCTACTGGGC AAGGCTGTTC 
     1261 GCTGCTATGA ATCTCTAATC TTAAAAGCTG AAGGAAAAGT GGAGTCTGAT TTCTTTTGTC 
     1321 AATTAGGTCA CTTCAACCTC TTATTGGAAG ATTATCCAAA AGCATTATCT GCATACCAGA 
     1381 GGTACTACAG TTTACAGTCT GACTACTGGA AGAATGCTGC CTTTTTATAT GGTCTTGGTT 
     1441 TGGTCTACTT CCATTATAAT GCATTTCAGT GGGCAATTAA AGCATTTCAG GAGGTGCTTT 
     1501 ATGTTGATCC CAGCTTTTGT CGAGCCAAGG AAATTCATTT ACGACTTGGG CTTATGTTCA 
     1561 AAGTGAACAC AGACTATGAG TCTAGTTTAA AGCATTTTCA GTTAGCTTTG GTTGACTGTA 
     1621 ATCCCTGCAC TTTGTCCAAT GCTGAAATTC AATTTCACAT TGCCCACTTA TATGAAACCC 
     1681 AGAGGAAATA TCATTCTGCA AAAGAAGCTT ATGAACAACT TTTGCAGACA GAGAATCTTT 
     1741 CTGCACAAGT AAAAGCAACT GTCTTACAAC AGTTAGGTTG GATGCATCAC ACTGTAGATC 
     1801 TCCTGGGAGA TAAAGCCACC AAGGAAAGCT ATGCTATTCA GTATCTCCAA AAGTCCTTGG 
     1861 AAGCAGATCC TAATTCTGGC CAGTCCTGGT ATTTCCTCGG AAGGTGCTAT TCAAGTATTG 
     1921 GGAAAGTTCA GGATGCCTTT ATATCTTACA GGCAGTCTAT TGATAAATCA GAAGCAAGTG 
     1981 CAGATACATG GTGTTCAATA GGTGTGCTAT ATCAGCAGCA AAATCAGCCC ATGGATGCTT 
     2041 TACAGGCCTA TATTTGTGCT GTACAATTGG ACCATGGCCA TGCTGCAGCC TGGATGGACC 
     2101 TAGGCACTCT CTATGAATCC TGCAACCAGC CTCAGGATGC CATTAAATGC TACTTAAATG 
     2161 CAACTAGAAG CAAAAGTTGT AGTAATACCT CTGCACTTGC AGCACGAATT AAGTATTTAC 
     2221 AGAATACTTC TGACAATTGG AGTGGTGGAC ATGCTGTGTC ACATCCTCCA GTACAGCAAC 
     2281 AAGCTCATTC ATGGTGTTTG ACACCACAGA AATTACAGCA TTTGGAACAG CTCCGCGCAA 
     2341 ATAGAAATAA TTTAAATCCA GCACAGAAAC TGATGCTGGA ACAGCTGGAA AGTCAGTTTG 
     2401 TCTTAATGCA ACAACACCAA ATGAGACCAA CAGGAGTTGC ACAGGTACGA TCTACTGGAA 
     2461 TTCCTAATGG GCCAACAGCT GACTCATCAC TGCCTACAAA CTCAGTCTCT GGCCAGCAGC 
     2521 CACAGCTTGC TCTGACCAGA GTGCCTAGCG TCTCTCAGCC TGGAGTCCGT CCTGCCTGCC 
     2581 CTGGGCAGCC TTTGGCCAAT GGACCCTTTT CTGCAGGCCA TGTTCCCTGT AGCACATCAA 
     2641 GAACGCTGGG AAGTACAGAC ACTATTTTGA TAGGCAATAA TCATATAACA GGAAGTGGAA 
     2701 GTAATGGAAA CGTGCCTTAC CTGCAGCGAA ACGCACTCAC TCTACCTCAT AACCGCACAA 
     2761 ACCTGACCAG CAGCGCAGAG GAGCCGTGGA AAAACCAACT ATCTAACTCC ACTCAGGGGC 
     2821 TTCACAAAGG TCAGAGTTCA CATTCGGCAG GTCCTAATGG TGAACGACCT CTCTCTTCCA 
     2881 CTGGGCCTTC CCAGCATCTC CAGGCAGCTG GCTCTGGTAT TCAGAATCAG AACGGACATC 
     2941 CCACCCTGCC TAGCAATTCA GTAACACAGG GGGCTGCTCT CAATCACCTC TCCTCTCACA 
     3001 CTGCTACCTC AGGTGGACAA CAAGGCATTA CCTTAACCAA AGAGAGCAAG CCTTCAGGAA 
     3061 ACATATTGAC GGTGCCTGAA ACAAGCAGGC ACACTGGAGA GACACCTAAC AGCACTGCCA 
     3121 GTGTCGAGGG ACTTCCTAAT CATGTCCATC AGATGACGGC AGATGCTGTT TGCAGTCCTA 
     3181 GCCATGGAGA TTCTAAGTCA CCAGGTTTAC TAAGTTCAGA CAATCCTCAG CTCTCTGCCT 
     3241 TGTTGATGGG AAAAGCCAAT AACAATGTGG GTACTGGAAC CTGTGACAAA GTCAATAACA 
     3301 TCCACCCAGC TGTTCATACA AAGACTGATA ACTCTGTTGC CTCTTCACCA TCTTCAGCCA 
     3361 TTTCAACAGC AACACCTTCT CCAAAATCCA CTGAGCAGAC AACCACAAAC AGTGTTACCA 
     3421 GCCTTAACAG CCCTCACAGT GGGCTACACA CAATTAATGG AGAAGGGATG GAAGAATCTC 
     3481 AGAGCCCCAT GAAAACAGAT CTGCTTCTGG TTAACCACAA ACCTAGTCCA CAGATCATAC 
     3541 CATCAATGTC TGTGTCCATA TACCCCAGCT CAGCAGAAGT TCTGAAGGCA TGCAGGAATC 
     3601 TAGGTAAAAA TGGCTTATCT AACAGTAGCA TTTTGTTGGA TAAATGTCCA CCTCCAAGAC 
     3661 CACCATCTTC ACCATACCCT CCCTTGCCAA AGGACAAGTT GAATCCACCT ACACCTAGTA 
     3721 TTTACTTGGA AAATAAACGT GATGCTTTCT TTCCTCCATT ACATCAATTT TGTACAAATC 
     3781 CGAACAACCC TGTTACAGTA ATACGTGGCC TTGCTGGAGC TCTTAAGTTA GACCTGGGAC 
     3841 TTTTCTCTAC TAAAACTTTG GTGGAAGCTA ACAATGAACA TATGGTAGAA GTGAGGACAC 
     3901 AGTTGTTGCA GCCAGCAGAT GAAAACTGGG ATCCCACTGG AACAAAGAAA ATCTGGCATT 
     3961 GTGAAAGTAA TAGATCTCAT ACTACAATTG CTAAATATGC ACAGTACCAG GCCTCCTCAT 
     4021 TCCAGGAATC ATTGAGAGAA GAAAATGAAA AAAGAAGTCA TCATAAAGAC CACTCAGATA 
     4081 GTGAATCTAC ATCGTCAGAT AATTCTGGGA GGAGGAGGAA AGGACCCTTT AAAACCATAA 
     4141 AGTTTGGGAC CAATATTGAC CTATCTGATG ACAAAAAGTG GAAGTTGCAG CTACATGAGC 
     4201 TGACTAAACT TCCTGCTTTT GTGCGTGTCG TATCAGCAGG AAATCTTCTA AGCCATGTTG 
     4261 GTCATACCAT ATTGGGCATG AACACAGTTC AACTATACAT GAAAGTTCCA GGGAGCAGAA 
     4321 CACCAGGTCA TCAGGAAAAT AACAACTTCT GTTCAGTTAA CATAAATATT GGCCCAGGTG 
     4381 ACTGTGAATG GTTTGTTGTT CCTGAAGGTT ACTGGGGTGT TCTGAATGAC TTCTGTGAAA 
     4441 AAAATAATTT GAATTTCCTA ATGGGTTCTT GGTGGCCCAA TCTTGAAGAT CTTTATGAAG 
     4501 CAAATGTTCC AGTGTATAGG TTTATTCAGC GACCTGGAGA TTTGGTCTGG ATAAATGCAG 
     4561 GCACTGTTCA TTGGGTTCAG GCTATTGGCT GGTGCAACAA CATTGCTTGG AATGTTGGTC 
     4621 CACTTACAGC CTGCCAGTAT AAATTGGCAG TGGAACGGTA CGAATGGAAC AAATTGCAAA 
     4681 GTGTGAAGTC AATAGTACCC ATGGTTCATC TTTCCTGGAA TATGGCACGA AATATCAAGG 
     4741 TCTCAGATCC AAAGCTTTTT GAAATGATTA AGTATTGTCT TCTAAGAACT CTGAAGCAAT 
     4801 GTCAGACATT GAGGGAAGCT CTCATTGCTG CAGGAAAAGA GATTATATGG CATGGGCGGA 
     4861 CAAAAGAAGA ACCAGCTCAT TACTGTAGCA TTTGTGAAGT GGAGGTTTTT GATCTGCTTT 
     4921 TTGTCACTAA TGAGAGTAAT TCACGAAAGA CCTACATAGT ACATTGCCAA GATTGTGCAC 
     4981 GAAAAACAAG CGGAAACTTG GAAAACTTTG TGGTGCTAGA ACAGTACAAA ATGGAGGACC 
     5041 TGATGCAAGT CTATGACCAA TTTACATTAG CTCCTCCATT ACCATCCGCC TCATCTACGC 
     5101 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     5161 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     5221 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     5281 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5341 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5401 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5461 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5521 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5581 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5641 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5701 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5761 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5821 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5881 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5941 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     6001 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     6061 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     6121 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     6181 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     6241 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6301 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6361 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6421 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6481 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6541 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6601 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6661 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6721 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6781 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6841 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6901 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6961 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     7021 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     7081 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     7141 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     7201 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     7261 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7321 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7381 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7441 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7501 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7561 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7621 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7681 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7741 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7801 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7861 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7921 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7981 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     8041 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     8101 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     8161 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     8221 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     8281 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8341 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8401 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8461 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8521 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8581 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8641 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8701 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8761 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8821 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8881 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8941 GCGAATTTT
//