LOCUS       RC240111                8862 bp    DNA     circular  SYN 
DEFINITION  CEP162 (myc-DDK-tagged) - Human centrosomal protein 162kDa (CEP162), transcript variant 2
ACCESSION   NM_001286206
VERSION     NM_001286206.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5019..5110 
                     /label="Myc-DDK"  
     misc_feature    5140..5725 
                     /label="PolyA signal"  
     misc_feature    5197..5216 
                     /label="Restriction Enzyme"  
     misc_feature    6001..6673 
                     /label="ColE1"  
     misc_feature    7103..7897 
                     /label="Kan/Neo"  
     misc_feature    7932..8234 
                     /label="SV40 ori"  
     misc_feature    8320..8797 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5001..5007 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5001 
                     /label="CEP162"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAAATAGAA GAGGAGTCTG CTGAAAAGAT TCAATTTCTT AAGAGCAGTG 
     1081 GAACCTCTCT CTTAAGTACT GATAGCTTAG AAACAAATGA ACTAGTAGTT TCTGAGCTCA 
     1141 ACCATAGTAG TCTCGGAGTG GGATTGGACA CATTAGAAGA ACAAGAGGAG AAAGAACAAT 
     1201 TTTTTGCCAG GCTTGAGAAA GGCTTGACAT CTTCCATTGA TTATTCGAGA TTAAATAAGG 
     1261 AATTGGATTC TAATGACTCT ACACATTTTA AAGCTTTACA TAGTAATCAA GCCAACGCAG 
     1321 AACTAACTGA TGACGAACAT GAGAATGAAT CGAAACATGA AGAACTGGCA GAAAATTACA 
     1381 GTGATGATTT TGAAGATGAG TATGTTGGTG CACCGTTGAC TACTAAAGAT GAAGAGATGC 
     1441 CTTCCAAAGA GAATTCCAAA TCAGAAAAAA TAAGTGTGCC CAAACAGGAA GAAGAAAAAA 
     1501 CTGGCATGCT TGCTAATGTT GTGCTGCTTG ATTCATTAGA CTCTGTTGCA GAGGTCAATC 
     1561 TTGATGAACA AGATAAAATA ACACCTAAGC CAAGGTGCCT ACCAGAAATG ACTGAGAATG 
     1621 AAATGACAGG AACAGGTGTT TCTTATGGAC AAAGCAGTAG TGACGTTGAA GCCCTACATC 
     1681 AAGCTTATTG TCATATAGCC CATTCATTGG GAGATGAAGA CAAACAAAAA ATTGAGAGTA 
     1741 ACACAGTGGA AGATATCAAG AGCTCAGTGA AAGGTCATCC TCAAGAAAAT GAAGAGAATT 
     1801 CAAAAAACAT CTCTACTATG GAATCTGATC TGCCCACAGT AGAGGAGCTG ATGAAACCTA 
     1861 TCAGAATAGA TTCCTTTGGG ATCAGTGGTT TTGATTTACA ACCTGTCAGC TCTGAGAAAG 
     1921 TGGCCGAAAG AAAAGAAACT GAATTTTTTA GCTCTTTACC CCTGAAGATG AACCCAAATA 
     1981 TTTTGTCTCA AGACTCACAA CATGTGAACC TTTTTTTTGA CAAAAATGAT GAGAATGTGA 
     2041 TTTTACAAAA GACCACAAAT GAGAGTATGG AAAACAGCTG TCCACAAGTA ACTGAAGTAA 
     2101 CTGCCACAGA AGAACATGTT GATAAAATGT ACCTTAATAT TTTGAGGAAA AAAATAACTG 
     2161 TTAATTCTTC ATCATTATCT CAGGATGACA AAATTAATAA AACTTACAGA TCTCAACTTA 
     2221 GTTCTGAAGA AGAAGGGGCT GTAATGGGTA AACAGGTACC ATACAAGAAG GCCAGAAGTG 
     2281 CACCTCCTTT ACTTAAAAGG AAACCCCAGA GTGGATTATA TGCGTCAGTT AGGAGCTCAG 
     2341 GCTATGGCAA ACCCAGTTCA CCACTCAAGA TGTTTTCTAC TCTTGAAAAG AAAACTTCAG 
     2401 AGGACATTAT AAAAAGCAAA AACTTGAGAT CGATTTCTAC CTCCAATCAA CCTAGGAAAA 
     2461 AAGAAATCTT ATCTGGAACA AAACTCATCA AGCCTGCAGC TTTGGATAAA CCAGCTCACA 
     2521 AAACTGAAAG TTGCCTGTCT ACTCGTAAGA AGTCTGAAAA TCCCACAGAA ACTGATTCCT 
     2581 GTATTCAGTT TCAGACTGAT TCCTTAGGAT ACTGTGGTGA GAACAAGGAG AAGAAATTAC 
     2641 TTATGTTTAA AAGAGTTCAG GAAGCAGAGG ATAAATGGAG GGGTGCGCAA GCCCTAATTG 
     2701 AGCAAATTAA AGCCACATTC TCAGAAAAGG AGAAAGAACT AGAAAATAAG TTGGAAGAAC 
     2761 TAAAGAAACA ACAGGAAAAA GAACTCTTCA AATTGAATCA AGATAATTAT ATTCTTCAAG 
     2821 CCAAGTTAAG CAGCTTTGAA GAAACAAACA AAAAACAAAG GTGGTTACAT TTTGGAGAAG 
     2881 CAGCTGATCC TGTCACTGGA GAAAAGTTGA AGCAAATCCA AAAAGAAATA CAAGAACAAG 
     2941 AGACACTTCT TCAAGGATAT CAACAGGAAA ATGAACGATT ATATAATCAA GTAAAAGATC 
     3001 TCCAAGAACA AAACAAGAAA AATGAGGAGC GAATGTTTAA GGAAAACCAA AGTTTATTCA 
     3061 GTGAGGTAGC TTCCTTAAAA GAACAGATGC ACAAAAGTCG TTTTCTGTCT CAAGTAGTTG 
     3121 AAGATTCAGA GCCCACAAGA AATCAGAATT TTACAGATCT GTTAGCAGAA CTACGGATGG 
     3181 CACAGAAAGA AAAAGACAGT TTATTGGAAG ACATCAAAAG ACTGAAACAA GACAAACAAG 
     3241 CTCTTGAAGT AGACTTCGAA AAAATGAAGA AAGAGAGGGA CCAAGCCAAA GATCAGATTG 
     3301 CTTATGTCAC AGGTGAGAAA TTATATGAAA TAAAAATTTT AGAAGAAACA CATAAACAAG 
     3361 AAATCAGTCG TCTGCAAAAA AGATTACAGT GGTATGCTGA AAATCAGGAA CTTCTGGATA 
     3421 AAGATGCACT TCGGCTTAGA GAAGCAAATG AGGAAATTGA GAAGCTCAAA CTTGAGATTG 
     3481 AGAAACTGAA AGCTGAATCT GGGAATCCAT CTATTCGGCA GAAGATACGC TTAAAAGATA 
     3541 AAGCAGCAGA TGCCAAAAAA ATTCAGGATC TGGAGCGACA AGTTAAGGAA ATGGAAGGGA 
     3601 TTCTGAAGAG AAGATATCCC AATTCTTTAC CTGCTTTAAT ATTGGCTGCA TCAGCAGCTG 
     3661 GTGATACAGT GGATAAAAAT ACAGTGGAAT TTATGGAGAA AAGGATAAAA AAGCTAGAAG 
     3721 CTGATCTGGA GGGCAAAGAT GAAGATGCAA AGAAAAGCCT TCGTACCATG GAACAACAGT 
     3781 TTCAGAAAAT GAAGATTCAG TATGAACAAA GACTAGAGCA GCAGGAGCAG CTACTTGCCT 
     3841 GCAAATTGAA TCAACATGAC TCTCCCAGAA TTAAAGCCCT AGAGAAGGAA CTTGATGACA 
     3901 TCAAGGAAGC CCATCAGATC ACTGTAAGAA ACCTTGAAGC CGAAATAGAC GTTCTTAAAC 
     3961 ATCAGAATGC TGAATTAGAC GTCAAGAAAA ATGATAAAGA TGATGAAGAT TTTCAGTCTA 
     4021 TAGAATTCCA GGTGGAACAG GCTCATGCTA AAGCTAAATT AGTAAGACTC AATGAAGAAC 
     4081 TGGCTGCAAA AAAGAGAGAA ATACAAGACC TCTCAAAAAC TGTGGAGAGG CTTCAGAAAG 
     4141 ACAGAAGAAT GATGCTATCA AATCAGAACT CAAAGGGCAG AGAGGAAATG TCTGCCAAAA 
     4201 GGGCAAAGAA AGATGTTTTG CACTCAAGTA AAGGAAATGC TAACTCCTTC CCTGGAACCC 
     4261 TGGACAGCAA GCTGTACCAA CCACATACTT TCACTGATTC CCATGTTTCA GAAGTTTTAC 
     4321 AAGAAAACTA CAGATTAAAA AATGAGCTAG AAGGATTAAT TTCAGAGAAG AATGAACTGA 
     4381 AGATGAAATC TGAAGCAGTG ATGAATCAAT TTGAAAACTC CATGAGAAGG GTCAAGGAAG 
     4441 ATACTGCAGC ACACATTGCA TCCCTCAAAG CATCTCATCA GAGAGAAATA GAGAAACTCC 
     4501 TTTGCCAAAA TGCAGTAGAA AATTCTTCTT CCAAAGTAGC TGAACTAAAT CGTAAAATAG 
     4561 CAACTCAAGA GGTACTTATA AGACATTTCC AAAGTCAAGT TAATGAGCTG CAGAGTAAAC 
     4621 AAGAGTCTCT CGTAGTTTCT GAAGTTCGAG AAGAAATTCT ACAGAAAGAG ATTACAAAAC 
     4681 TCTTAGAAGA ATTGAGAGAA GCCAAAGAAA ACCATACACC AGAGATGAAA CATTTCGTGG 
     4741 GCTTAGAAAA GAAAATTAAG CAGATGGAAA TGAGACATGC ACAAAGAGAA CAGGAACTTC 
     4801 AACAGATAAT ACAGCAAACA CACCAAGTAG TAGAAACTGA GCAAAACAAA GAAGTTGAAA 
     4861 AATGGAAAAG ACTGGCACAG TTAAAGAATC GTGAGCTGGA GAAGTTCCGC ACAGAACTAG 
     4921 ACTCAATATT AGATGTTCTC CGAGAGCTGC ACCGGCAAGG AGTGGTTGTG CCAGTTGCTT 
     4981 TTGCAGATGA AATGAATGCA CCAGAGTATA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     5041 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     5101 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     5161 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     5221 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     5281 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     5341 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5401 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5461 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5521 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5581 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5641 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5701 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5761 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5821 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5881 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5941 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     6001 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     6061 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     6121 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     6181 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     6241 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     6301 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6361 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6421 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6481 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6541 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6601 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6661 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6721 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6781 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6841 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6901 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6961 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     7021 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     7081 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     7141 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     7201 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     7261 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     7321 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7381 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7441 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7501 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7561 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7621 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7681 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7741 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7801 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7861 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7921 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7981 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     8041 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     8101 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8161 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8221 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     8281 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     8341 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8401 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8461 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8521 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8581 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8641 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8701 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8761 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8821 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//