LOCUS       RC240108                8847 bp    DNA     circular  SYN 
DEFINITION  KDM6A (myc-DDK-tagged) - Human lysine (K)-specific demethylase 6A (KDM6A), transcript variant 5
ACCESSION   NM_001291418
VERSION     NM_001291418.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5004..5095 
                     /label="Myc-DDK"  
     misc_feature    5125..5710 
                     /label="PolyA signal"  
     misc_feature    5182..5201 
                     /label="Restriction Enzyme"  
     misc_feature    5986..6658 
                     /label="ColE1"  
     misc_feature    7088..7882 
                     /label="Kan/Neo"  
     misc_feature    7917..8219 
                     /label="SV40 ori"  
     misc_feature    8305..8782 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4986..4992 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4986 
                     /label="KDM6A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAATCCTGC GGAGTGTCGC TCGCTACCGC CGCCGCTGCC GCCGCCGCTT 
     1081 TCGGTGATGA GGAAAAGAAA ATGGCGGCGG GAAAAGCGAG CGGCGAGAGC GAGGAGGCGT 
     1141 CCCCCAGCCT GACAGCCGAG GAGAGGGAGG CGCTCGGCGG ACTGGACAGC CGCCTCTTTG 
     1201 GGTTCGTGAG ATTTCATGAA GATGGCGCCA GGACGAAGGC CCTACTGGGC AAGGCTGTTC 
     1261 GCTGCTATGA ATCTCTAATC TTAAAAGCTG AAGGAAAAGT GGAGTCTGAT TTCTTTTGTC 
     1321 AATTAGGTCA CTTCAACCTC TTATTGGAAG ATTATCCAAA AGCATTATCT GCATACCAGA 
     1381 GGTACTACAG TTTACAGTCT GACTACTGGA AGAATGCTGC CTTTTTATAT GGTCTTGGTT 
     1441 TGGTCTACTT CCATTATAAT GCATTTCAGT GGGCAATTAA AGCATTTCAG GAGGTGCTTT 
     1501 ATGTTGATCC CAGCTTTTGT CGAGCCAAGG AAATTCATTT ACGACTTGGG CTTATGTTCA 
     1561 AAGTGAACAC AGACTATGAG TCTAGTTTAA AGCATTTTCA GTTAGCTTTG GTTGACTGTA 
     1621 ATCCCTGCAC TTTGTCCAAT GCTGAAATTC AATTTCACAT TGCCCACTTA TATGAAACCC 
     1681 AGAGGAAATA TCATTCTGCA AAAGAAGCTT ATGAACAACT TTTGCAGACA GAGAATCTTT 
     1741 CTGCACAAGT AAAAGCAACT GTCTTACAAC AGTTAGGTTG GATGCATCAC ACTGTAGATC 
     1801 TCCTGGGAGA TAAAGCCACC AAGGAAAGCT ATGCTATTCA GTATCTCCAA AAGTCCTTGG 
     1861 AAGCAGATCC TAATTCTGGC CAGTCCTGGT ATTTCCTCGG AAGGTGCTAT TCAAGTATTG 
     1921 GGAAAGTTCA GGATGCCTTT ATATCTTACA GGCAGTCTAT TGATAAATCA GAAGCAAGTG 
     1981 CAGATACATG GTGTTCAATA GGTGTGCTAT ATCAGCAGCA AAATCAGCCC ATGGATGCTT 
     2041 TACAGGCCTA TATTTGTGCT GTACAATTGG ACCATGGCCA TGCTGCAGCC TGGATGGACC 
     2101 TAGGCACTCT CTATGAATCC TGCAACCAGC CTCAGGATGC CATTAAATGC TACTTAAATG 
     2161 CAACTAGAAG CAAAAGTTGT AGTAATACCT CTGCACTTGC AGCACGAATT AAGTATTTAC 
     2221 AGAATACTTC TGACAATTGG AGTGGTGGAC ATGCTGTGTC ACATCCTCCA GTACAGCAAC 
     2281 AAGCTCATTC ATGGTGTTTG ACACCACAGA AATTACAGAT GAGACCAACA GGAGTTGCAC 
     2341 AGGTACGATC TACTGGAATT CCTAATGGGC CAACAGCTGA CTCATCACTG CCTACAAACT 
     2401 CAGTCTCTGG CCAGCAGCCA CAGCTTGCTC TGACCAGAGT GCCTAGCGTC TCTCAGCCTG 
     2461 GAGTCCGTCC TGCCTGCCCT GGGCAGCCTT TGGCCAATGG ACCCTTTTCT GCAGGCCATG 
     2521 TTCCCTGTAG CACATCAAGA ACGCTGGGAA GTACAGACAC TATTTTGATA GGCAATAATC 
     2581 ATATAACAGG AAGTGGAAGT AATGGAAACG TGCCTTACCT GCAGCGAAAC GCACTCACTC 
     2641 TACCTCATAA CCGCACAAAC CTGACCAGCA GCGCAGAGGA GCCGTGGAAA AACCAACTAT 
     2701 CTAACTCCAC TCAGGGGCTT CACAAAGGTC AGAGTTCACA TTCGGCAGGT CCTAATGGTG 
     2761 AACGACCTCT CTCTTCCACT GGGCCTTCCC AGCATCTCCA GGCAGCTGGC TCTGGTATTC 
     2821 AGAATCAGAA CGGACATCCC ACCCTGCCTA GCAATTCAGT AACACAGGGG GCTGCTCTCA 
     2881 ATCACCTCTC CTCTCACACT GCTACCTCAG GTGGACAACA AGGCATTACC TTAACCAAAG 
     2941 AGAGCAAGCC TTCAGGAAAC ATATTGACGG TGCCTGAAAC AAGCAGGCAC ACTGGAGAGA 
     3001 CACCTAACAG CACTGCCAGT GTCGAGGGAC TTCCTAATCA TGTCCATCAG ATGACGGCAG 
     3061 ATGCTGTTTG CAGTCCTAGC CATGGAGATT CTAAGTCACC AGGTTTACTA AGTTCAGACA 
     3121 ATCCTCAGCT CTCTGCCTTG TTGATGGGAA AAGCCAATAA CAATGTGGGT ACTGGAACCT 
     3181 GTGACAAAGT CAATAACATC CACCCAGCTG TTCATACAAA GACTGATAAC TCTGTTGCCT 
     3241 CTTCACCATC TTCAGCCATT TCAACAGCAA CACCTTCTCC AAAATCCACT GAGCAGACAA 
     3301 CCACAAACAG TGTTACCAGC CTTAACAGCC CTCACAGTGG GCTACACACA ATTAATGGAG 
     3361 AAGGGATGGA AGAATCTCAG AGCCCCATGA AAACAGATCT GCTTCTGGTT AACCACAAAC 
     3421 CTAGTCCACA GATCATACCA TCAATGTCTG TGTCCATATA CCCCAGCTCA GCAGAAGTTC 
     3481 TGAAGGCATG CAGGAATCTA GGTAAAAATG GCTTATCTAA CAGTAGCATT TTGTTGGATA 
     3541 AATGTCCACC TCCAAGACCA CCATCTTCAC CATACCCTCC CTTGCCAAAG GACAAGTTGA 
     3601 ATCCACCTAC ACCTAGTATT TACTTGGAAA ATAAACGTGA TGCTTTCTTT CCTCCATTAC 
     3661 ATCAATTTTG TACAAATCCG AACAACCCTG TTACAGTAAT ACGTGGCCTT GCTGGAGCTC 
     3721 TTAAGTTAGA CCTGGGACTT TTCTCTACTA AAACTTTGGT GGAAGCTAAC AATGAACATA 
     3781 TGGTAGAAGT GAGGACACAG TTGTTGCAGC CAGCAGATGA AAACTGGGAT CCCACTGGAA 
     3841 CAAAGAAAAT CTGGCATTGT GAAAGTAATA GATCTCATAC TACAATTGCT AAATATGCAC 
     3901 AGTACCAGGC CTCCTCATTC CAGGAATCAT TGAGAGAAGA AAATGAAAAA AGAAGTCATC 
     3961 ATAAAGACCA CTCAGATAGT GAATCTACAT CGTCAGATAA TTCTGGGAGG AGGAGGAAAG 
     4021 GACCCTTTAA AACCATAAAG TTTGGGACCA ATATTGACCT ATCTGATGAC AAAAAGTGGA 
     4081 AGTTGCAGCT ACATGAGCTG ACTAAACTTC CTGCTTTTGT GCGTGTCGTA TCAGCAGGAA 
     4141 ATCTTCTAAG CCATGTTGGT CATACCATAT TGGGCATGAA CACAGTTCAA CTATACATGA 
     4201 AAGTTCCAGG GAGCAGAACA CCAGGTCATC AGGAAAATAA CAACTTCTGT TCAGTTAACA 
     4261 TAAATATTGG CCCAGGTGAC TGTGAATGGT TTGTTGTTCC TGAAGGTTAC TGGGGTGTTC 
     4321 TGAATGACTT CTGTGAAAAA AATAATTTGA ATTTCCTAAT GGGTTCTTGG TGGCCCAATC 
     4381 TTGAAGATCT TTATGAAGCA AATGTTCCAG TGTATAGGTT TATTCAGCGA CCTGGAGATT 
     4441 TGGTCTGGAT AAATGCAGGC ACTGTTCATT GGGTTCAGGC TATTGGCTGG TGCAACAACA 
     4501 TTGCTTGGAA TGTTGGTCCA CTTACAGCCT GCCAGTATAA ATTGGCAGTG GAACGGTACG 
     4561 AATGGAACAA ATTGCAAAGT GTGAAGTCAA TAGTACCCAT GGTTCATCTT TCCTGGAATA 
     4621 TGGCACGAAA TATCAAGGTC TCAGATCCAA AGCTTTTTGA AATGATTAAG TATTGTCTTC 
     4681 TAAGAACTCT GAAGCAATGT CAGACATTGA GGGAAGCTCT CATTGCTGCA GGAAAAGAGA 
     4741 TTATATGGCA TGGGCGGACA AAAGAAGAAC CAGCTCATTA CTGTAGCATT TGTGAAGTGG 
     4801 AGGTTTTTGA TCTGCTTTTT GTCACTAATG AGAGTAATTC ACGAAAGACC TACATAGTAC 
     4861 ATTGCCAAGA TTGTGCACGA AAAACAAGCG GAAACTTGGA AAACTTTGTG GTGCTAGAAC 
     4921 AGTACAAAAT GGAGGACCTG ATGCAAGTCT ATGACCAATT TACATTAGCT CCTCCATTAC 
     4981 CATCCGCCTC ATCTACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     5041 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     5101 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     5161 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     5221 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     5281 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5341 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5401 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5461 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5521 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5581 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5641 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5701 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5761 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5821 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5881 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5941 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     6001 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     6061 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     6121 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     6181 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     6241 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6301 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6361 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6421 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6481 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6541 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6601 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6661 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6721 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6781 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6841 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6901 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6961 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     7021 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     7081 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     7141 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     7201 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     7261 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7321 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7381 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7441 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7501 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7561 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7621 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7681 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7741 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7801 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7861 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7921 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7981 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     8041 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     8101 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8161 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8221 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     8281 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8341 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8401 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8461 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8521 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8581 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8641 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8701 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8761 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8821 ATTTAACAAA AATTTAACGC GAATTTT
//