LOCUS       RC240106                8838 bp    DNA     circular  SYN 
DEFINITION  NEDD4 (myc-DDK-tagged) - Human neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant 3
ACCESSION   NM_001284338
VERSION     NM_001284338.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4995..5086 
                     /label="Myc-DDK"  
     misc_feature    5116..5701 
                     /label="PolyA signal"  
     misc_feature    5173..5192 
                     /label="Restriction Enzyme"  
     misc_feature    5977..6649 
                     /label="ColE1"  
     misc_feature    7079..7873 
                     /label="Kan/Neo"  
     misc_feature    7908..8210 
                     /label="SV40 ori"  
     misc_feature    8296..8773 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4977..4983 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4977 
                     /label="NEDD4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCACAAAGC TTACGATTGC ACTTTGCAGC CAGAAGAAGC AATACTTACC 
     1081 CTTTGTCAGA AACCTCCGGA GATGACTTGG ATAGCCATGT TCACATGTGC TTCAAAAGAC 
     1141 CAACACGGAT TTCAACGTCT AACGTTGTTC AAATGAAGCT GACTCCCAGA CAGACTGCAC 
     1201 TAGCTCCGTT AATAAAGGAA AACGTTCAGT CTCAAGAAAG ATCATCTGTT CCCTCATCTG 
     1261 AAAATGTTAA TAAAAAGAGC AGCTGTCTAC AGATTTCACT ACAGCCAACA AGGTACAGTG 
     1321 GATATCTTCA GTCTAGCAAT GTCTTAGCTG ATAGTGATGA TGCTTCGTTT ACTTGTATCT 
     1381 TGAAGGATGG TATTTACAGT AGTGCTGTGG TCGATAATGA ATTGAATGCT GTGAATGATG 
     1441 GTCACCTTGT AAGCAGTCCA GCCATTTGTA GTGGTAGCCT TAGTAACTTT TCAACCAGTG 
     1501 ATAATGGGTC TTACAGCAGC AACGGTAGTG ATTTTGGGTC ATGTGCAAGT ATCACAAGTG 
     1561 GAGGTTCATA TACTAACAGT GTCATCAGTG ACAGTAGTAG TTATACTTTT CCACCAAGTG 
     1621 ATGATACTTT TTTGGGTGGA AACTTACCTT CTGACAGCAC CTCCAATAGA AGTGTGCCAA 
     1681 ACAGGAATAC TACTCCTTGT GAAATTTTTT CAAGAAGTAC AAGTACAGAT CCTTTTGTCC 
     1741 AGGATGACTT GGAACATGGA TTAGAGATTA TGAAATTGCC AGTGAGCAGG AACACAAAAA 
     1801 TTCCACTAAA ACGTTACTCC TCCTTAGTCA TTTTTCCTAG GAGTCCTTCA ACTACCCGAC 
     1861 CGACTTCTCC AACAAGTCTG TGTACTCTTC TGAGCAAAGG ATCCTATCAA ACTTCACACC 
     1921 AGTTTATTAT TTCTCCTAGT GAAATTGCAC ATAATGAGGA TGGCACTAGT GCTAAAGGAT 
     1981 TTCTTTCAAC AGCTGTCAAT GGACTTCGGT TATCTAAAAC AATTTGTACC CCCGGAGAAG 
     2041 TAAGAGACAT ACGGCCGCTT CACAGGAAGG GCTCGTTACA GAAGAAAATT GTTCTTTCGA 
     2101 ATAATACTCC CAGACAGACT GTCTGTGAAA AGTCATCTGA AGGATATTCT TGTGTTTCAG 
     2161 TGCATTTCAC CCAACGAAAA GCAGCTACAT TAGACTGTGA AACAACAAAT GGTGATTGTA 
     2221 AACCAGAAAT GTCAGAAATT AAGCTTAATT CTGATTCAGA GTATATTAAG CTCATGCATA 
     2281 GGACATCTGC ATGTTTGCCA TCCTCCCAAA ATGTAGATTG TCAAATAAAT ATCAATGGAG 
     2341 AATTGGAAAG ACCACATTCA CAGATGAACA AAAACCATGG TATTTTACGA AGAAGTATTT 
     2401 CATTGGGAGG AGCTTATCCA AATATTTCCT GTCTATCCAG CCTTAAGCAC AATTGTTCTA 
     2461 AAGGGGGACC ATCTCAGTTA CTCATAAAGT TTGCATCTGG AAATGAAGGT AAAGTGGATA 
     2521 ATTTATCAAG AGACAGCAAC AGAGATTGCA CAAATGAACT GTCTAATTCT TGCAAGACAA 
     2581 GAGATGATTT CCTAGGTCAA GTGGATGTTC CACTTTATCC ATTACCGACA GAAAATCCAA 
     2641 GATTGGAGAG ACCATATACA TTTAAGGATT TTGTTCTTCA TCCAAGAAGT CACAAATCAA 
     2701 GAGTTAAAGG TTATCTGAGA CTAAAAATGA CTTATTTACC TAAAACCAGT GGCTCAGAAG 
     2761 ATGATAATGC AGAACAGGCT GAGGAATTAG AGCCTGGCTG GGTTGTTTTG GACCAACCAG 
     2821 ATGCTGCTTG CCATTTGCAG CAACAACAAG AACCTTCTCC TCTACCTCCA GGGTGGGAAG 
     2881 AGAGGCAGGA TATCCTTGGA AGGACCTATT ATGTAAACCA TGAATCTAGA AGAACACAGT 
     2941 GGAAAAGACC AACCCCTCAG GACAACCTAA CAGATGCTGA GAATGGCAAC ATTCAACTGC 
     3001 AAGCACAACG TGCATTTACC ACCAGGCGGC AGATATCCGA GGAAACAGAA AGTGTTGACA 
     3061 ACCGAGAGTC TTCCGAGAAC TGGGAAATTA TAAGAGAAGA TGAAGCCACC ATGTATAGCA 
     3121 ACCAGGCCTT CCCATCACCT CCACCGTCAA GTAACTTGGA TGTTCCAACT CATCTTGCAG 
     3181 AAGAATTGAA TGCCAGACTC ACCATTTTTG GAAATTCAGC CGTGAGCCAG CCAGCATCGA 
     3241 GCTCAAATCA TTCCAGCAGA AGAGGCAGCT TACAAGCCTA TACTTTTGAG GAACAACCTA 
     3301 CACTTCCTGT GCTTTTGCCT ACTTCATCTG GATTACCACC AGGTTGGGAA GAAAAACAAG 
     3361 ATGAAAGAGG AAGATCATAT TATGTAGATC ACAATTCCAG AACGACTACT TGGACAAAGC 
     3421 CCACTGTACA GGCCACAGTG GAGACCAGTC AGCTGACCTC AAGCCAGAGT TCTGCAGGCC 
     3481 CTCAATCACA AGCCTCCACC AGTGATTCAG GCCAGCAGGT GACCCAGCCA TCTGAAATTG 
     3541 AGCAAGGATT CCTTCCTAAA GGCTGGGAAG TCCGGCATGC ACCAAATGGG AGGCCTTTCT 
     3601 TTATTGACCA CAACACTAAA ACCACCACCT GGGAAGATCC AAGATTGAAA ATTCCAGCCC 
     3661 ATCTGAGAGG AAAGACATCA CTTGATACTT CCAATGATCT AGGGCCTTTA CCTCCAGGAT 
     3721 GGGAAGAGAG AACTCACACA GATGGAAGAA TCTTCTACAT AAATCACAAT ATAAAAAGAA 
     3781 CACAATGGGA AGATCCTCGG TTGGAGAATG TAGCAATAAC TGGACCAGCA GTGCCCTACT 
     3841 CCAGGGATTA CAAAAGAAAG TATGAGTTCT TCCGAAGAAA GTTGAAGAAG CAGAATGACA 
     3901 TTCCAAACAA ATTTGAAATG AAACTTCGCC GAGCAACTGT TCTTGAAGAC TCTTACCGGA 
     3961 GAATTATGGG TGTCAAGAGA GCAGACTTCC TGAAGGCTCG ACTGTGGATT GAGTTTGATG 
     4021 GTGAAAAGGG ATTGGATTAT GGAGGAGTTG CCAGAGAATG GTTCTTCCTG ATCTCAAAGG 
     4081 AAATGTTTAA CCCTTATTAT GGGTTGTTTG AATATTCTGC TACGGACAAT TATACCCTAC 
     4141 AGATAAATCC AAACTCTGGA TTGTGTAACG AAGATCACCT CTCTTACTTC AAGTTTATTG 
     4201 GTCGGGTAGC TGGAATGGCA GTTTATCATG GCAAACTGTT GGATGGTTTT TTCATCCGCC 
     4261 CATTTTACAA GATGATGCTT CACAAACCAA TAACCCTTCA TGATATGGAA TCTGTGGATA 
     4321 GTGAATATTA CAATTCCCTA AGATGGATTC TTGAAAATGA CCCAACAGAA TTGGACCTCA 
     4381 GGTTTATCAT AGATGAAGAA CTTTTTGGAC AGACACATCA ACATGAGCTG AAAAATGGTG 
     4441 GATCAGAAAT AGTTGTCACC AATAAGAACA AAAAGGAATA TATTTATCTT GTAATACAAT 
     4501 GGCGATTTGT AAACCGAATC CAGAAGCAAA TGGCTGCTTT TAAAGAGGGA TTCTTTGAAC 
     4561 TAATACCACA GGATCTCATC AAAATTTTTG ATGAAAATGA ACTAGAGCTT CTTATGTGTG 
     4621 GACTGGGAGA TGTTGATGTG AATGACTGGA GGGAACATAC AAAGTATAAA AATGGCTACA 
     4681 GTGCAAATCA TCAGGTTATA CAGTGGTTTT GGAAGGCTGT TTTAATGATG GATTCAGAAA 
     4741 AAAGAATAAG ATTACTTCAG TTTGTCACTG GCACATCTCG GGTGCCTATG AATGGATTTG 
     4801 CTGAACTATA CGGTTCAAAT GGACCACAGT CATTTACAGT TGAACAGTGG GGTACTCCTG 
     4861 AAAAGCTGCC AAGAGCTCAT ACCTGTTTTA ATCGCCTGGA CTTGCCACCT TATGAATCAT 
     4921 TTGAAGAATT ATGGGATAAA CTTCAGATGG CAATTGAAAA CACCCAGGGC TTTGATGGAG 
     4981 TTGATACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     5041 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     5101 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     5161 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     5221 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     5281 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5341 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5401 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5461 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5521 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5581 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5641 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5701 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5761 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5821 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5881 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5941 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     6001 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     6061 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     6121 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     6181 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     6241 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6301 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6361 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6421 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6481 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6541 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6601 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6661 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6721 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6781 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6841 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6901 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6961 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     7021 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     7081 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     7141 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     7201 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     7261 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7321 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7381 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7441 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7501 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7561 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7621 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7681 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7741 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7801 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7861 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7921 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7981 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     8041 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     8101 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8161 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8221 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     8281 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8341 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8401 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8461 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8521 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8581 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8641 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8701 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8761 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8821 AAATTTAACG CGAATTTT
//