LOCUS       RC240103                8790 bp    DNA     circular  SYN 
DEFINITION  NEDD4 (myc-DDK-tagged) - Human neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant 4
ACCESSION   NM_001284339
VERSION     NM_001284339.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4947..5038 
                     /label="Myc-DDK"  
     misc_feature    5068..5653 
                     /label="PolyA signal"  
     misc_feature    5125..5144 
                     /label="Restriction Enzyme"  
     misc_feature    5929..6601 
                     /label="ColE1"  
     misc_feature    7031..7825 
                     /label="Kan/Neo"  
     misc_feature    7860..8162 
                     /label="SV40 ori"  
     misc_feature    8248..8725 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4929..4935 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4929 
                     /label="NEDD4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCACAAAGC TTACGATTGC ACTTTGCAGC CAGAAGAAGC AATACTTACC 
     1081 CTTTGTCAGA AACCTCCGGA GATGACTTGG ATAGCCATGT TCACATGTGC TTCAAAAGAC 
     1141 CAACACGGAT TTCAACGTCT AACGTTGTTC AAATGAAGCT GACTCCCAGA CAGACTGCAC 
     1201 TAGCTCCGTT AATAAAGGAA AACGTTCAGT CTCAAGAAAG ATCATCTGTT CCCTCATCTG 
     1261 AAAATGTTAA TAAAAAGAGC AGCTGTCTAC AGATTTCACT ACAGCCAACA AGGTACAGTG 
     1321 GATATCTTCA GTCTAGCAAT GTCTTAGCTG ATAGTGATGA TGCTTCGTTT ACTTGTATCT 
     1381 TGAAGGATGG TATTTACAGT AGTGCTGTGG TCGATAATGA ATTGAATGCT GTGAATGATG 
     1441 GTCACCTTGT AAGCAGTCCA GCCATTTGTA GTGGTAGCCT TAGTAACTTT TCAACCAGTG 
     1501 ATAATGGGTC TTACAGCAGC AACGGTAGTG ATTTTGGGTC ATGTGCAAGT ATCACAAGTG 
     1561 GAGGTTCATA TACTAACAGT GTCATCAGTG ACAGTAGTAG TTATACTTTT CCACCAAGTG 
     1621 ATGATACTTT TTTGGGTGGA AACTTACCTT CTGACAGCAC CTCCAATAGA AGTGTGCCAA 
     1681 ACAGGAATAC TACTCCTTGT GAAATTTTTT CAAGAAGTAC AAGTACAGAT CCTTTTGTCC 
     1741 AGGATGACTT GGAACATGGA TTAGAGATTA TGAAATTGCC AGTGAGCAGG AACACAAAAA 
     1801 TTCCACTAAA ACGTTACTCC TCCTTAGTCA TTTTTCCTAG GAGTCCTTCA ACTACCCGAC 
     1861 CGACTTCTCC AACAAGTCTG TGTACTCTTC TGAGCAAAGG ATCCTATCAA ACTTCACACC 
     1921 AGTTTATTAT TTCTCCTAGT GAAATTGCAC ATAATGAGGA TGGCACTAGT GCTAAAGGAT 
     1981 TTCTTTCAAC AGCTGTCAAT GGACTTCGGT TATCTAAAAC AATTTGTACC CCCGGAGAAG 
     2041 TAAGAGACAT ACGGCCGCTT CACAGGAAGG GCTCGTTACA GAAGAAAATT GTTCTTTCGA 
     2101 ATAATACTCC CAGACAGACT GTCTGTGAAA AGTCATCTGA AGGATATTCT TGTGTTTCAG 
     2161 TGCATTTCAC CCAACGAAAA GCAGCTACAT TAGACTGTGA AACAACAAAT GGTGATTGTA 
     2221 AACCAGAAAT GTCAGAAATT AAGCTTAATT CTGATTCAGA GTATATTAAG CTCATGCATA 
     2281 GGACATCTGC ATGTTTGCCA TCCTCCCAAA ATGTAGATTG TCAAATAAAT ATCAATGGAG 
     2341 AATTGGAAAG ACCACATTCA CAGATGAACA AAAACCATGG TATTTTACGA AGAAGTATTT 
     2401 CATTGGGAGG AGCTTATCCA AATATTTCCT GTCTATCCAG CCTTAAGCAC AATTGTTCTA 
     2461 AAGGGGGACC ATCTCAGTTA CTCATAAAGT TTGCATCTGG AAATGAAGGT AAAGTGGATA 
     2521 ATTTATCAAG AGACAGCAAC AGAGATTGCA CAAATGAACT GTCTAATTCT TGCAAGACAA 
     2581 GAGATGATTT CCTAGGTCAA GTGGATGTTC CACTTTATCC ATTACCGACA GAAAATCCAA 
     2641 GATTGGAGAG ACCATATACA TTTAAGGATT TTGTTCTTCA TCCAAGAAGT CACAAATCAA 
     2701 GAGTTAAAGG TTATCTGAGA CTAAAAATGA CTTATTTACC TAAAACCAGT GGCTCAGAAG 
     2761 ATGATAATGC AGAACAGGCT GAGGAATTAG AGCAACAACA AGAACCTTCT CCTCTACCTC 
     2821 CAGGGTGGGA AGAGAGGCAG GATATCCTTG GAAGGACCTA TTATGTAAAC CATGAATCTA 
     2881 GAAGAACACA GTGGAAAAGA CCAACCCCTC AGGACAACCT AACAGATGCT GAGAATGGCA 
     2941 ACATTCAACT GCAAGCACAA CGTGCATTTA CCACCAGGCG GCAGATATCC GAGGAAACAG 
     3001 AAAGTGTTGA CAACCGAGAG TCTTCCGAGA ACTGGGAAAT TATAAGAGAA GATGAAGCCA 
     3061 CCATGTATAG CAACCAGGCC TTCCCATCAC CTCCACCGTC AAGTAACTTG GATGTTCCAA 
     3121 CTCATCTTGC AGAAGAATTG AATGCCAGAC TCACCATTTT TGGAAATTCA GCCGTGAGCC 
     3181 AGCCAGCATC GAGCTCAAAT CATTCCAGCA GAAGAGGCAG CTTACAAGCC TATACTTTTG 
     3241 AGGAACAACC TACACTTCCT GTGCTTTTGC CTACTTCATC TGGATTACCA CCAGGTTGGG 
     3301 AAGAAAAACA AGATGAAAGA GGAAGATCAT ATTATGTAGA TCACAATTCC AGAACGACTA 
     3361 CTTGGACAAA GCCCACTGTA CAGGCCACAG TGGAGACCAG TCAGCTGACC TCAAGCCAGA 
     3421 GTTCTGCAGG CCCTCAATCA CAAGCCTCCA CCAGTGATTC AGGCCAGCAG GTGACCCAGC 
     3481 CATCTGAAAT TGAGCAAGGA TTCCTTCCTA AAGGCTGGGA AGTCCGGCAT GCACCAAATG 
     3541 GGAGGCCTTT CTTTATTGAC CACAACACTA AAACCACCAC CTGGGAAGAT CCAAGATTGA 
     3601 AAATTCCAGC CCATCTGAGA GGAAAGACAT CACTTGATAC TTCCAATGAT CTAGGGCCTT 
     3661 TACCTCCAGG ATGGGAAGAG AGAACTCACA CAGATGGAAG AATCTTCTAC ATAAATCACA 
     3721 ATATAAAAAG AACACAATGG GAAGATCCTC GGTTGGAGAA TGTAGCAATA ACTGGACCAG 
     3781 CAGTGCCCTA CTCCAGGGAT TACAAAAGAA AGTATGAGTT CTTCCGAAGA AAGTTGAAGA 
     3841 AGCAGAATGA CATTCCAAAC AAATTTGAAA TGAAACTTCG CCGAGCAACT GTTCTTGAAG 
     3901 ACTCTTACCG GAGAATTATG GGTGTCAAGA GAGCAGACTT CCTGAAGGCT CGACTGTGGA 
     3961 TTGAGTTTGA TGGTGAAAAG GGATTGGATT ATGGAGGAGT TGCCAGAGAA TGGTTCTTCC 
     4021 TGATCTCAAA GGAAATGTTT AACCCTTATT ATGGGTTGTT TGAATATTCT GCTACGGACA 
     4081 ATTATACCCT ACAGATAAAT CCAAACTCTG GATTGTGTAA CGAAGATCAC CTCTCTTACT 
     4141 TCAAGTTTAT TGGTCGGGTA GCTGGAATGG CAGTTTATCA TGGCAAACTG TTGGATGGTT 
     4201 TTTTCATCCG CCCATTTTAC AAGATGATGC TTCACAAACC AATAACCCTT CATGATATGG 
     4261 AATCTGTGGA TAGTGAATAT TACAATTCCC TAAGATGGAT TCTTGAAAAT GACCCAACAG 
     4321 AATTGGACCT CAGGTTTATC ATAGATGAAG AACTTTTTGG ACAGACACAT CAACATGAGC 
     4381 TGAAAAATGG TGGATCAGAA ATAGTTGTCA CCAATAAGAA CAAAAAGGAA TATATTTATC 
     4441 TTGTAATACA ATGGCGATTT GTAAACCGAA TCCAGAAGCA AATGGCTGCT TTTAAAGAGG 
     4501 GATTCTTTGA ACTAATACCA CAGGATCTCA TCAAAATTTT TGATGAAAAT GAACTAGAGC 
     4561 TTCTTATGTG TGGACTGGGA GATGTTGATG TGAATGACTG GAGGGAACAT ACAAAGTATA 
     4621 AAAATGGCTA CAGTGCAAAT CATCAGGTTA TACAGTGGTT TTGGAAGGCT GTTTTAATGA 
     4681 TGGATTCAGA AAAAAGAATA AGATTACTTC AGTTTGTCAC TGGCACATCT CGGGTGCCTA 
     4741 TGAATGGATT TGCTGAACTA TACGGTTCAA ATGGACCACA GTCATTTACA GTTGAACAGT 
     4801 GGGGTACTCC TGAAAAGCTG CCAAGAGCTC ATACCTGTTT TAATCGCCTG GACTTGCCAC 
     4861 CTTATGAATC ATTTGAAGAA TTATGGGATA AACTTCAGAT GGCAATTGAA AACACCCAGG 
     4921 GCTTTGATGG AGTTGATACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4981 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5041 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5101 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5161 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5221 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5281 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5341 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5401 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5461 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5521 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5581 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5641 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5701 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5761 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5821 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5881 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5941 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6001 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6061 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6121 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6181 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6241 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6301 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6361 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6421 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6481 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6541 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6601 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6661 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6721 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6781 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6841 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6901 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6961 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7021 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7081 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7141 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7201 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7261 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7321 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7381 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7441 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7501 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7561 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7621 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7681 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7741 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7801 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7861 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7921 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7981 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8041 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8101 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8161 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8221 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8281 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8341 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8401 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8461 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8521 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8581 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8641 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8701 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8761 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//