LOCUS       RC240102                8787 bp    DNA     circular  SYN 
DEFINITION  NEDD4 (myc-DDK-tagged) - Human neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant 5
ACCESSION   NM_001284340
VERSION     NM_001284340.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4944..5035 
                     /label="Myc-DDK"  
     misc_feature    5065..5650 
                     /label="PolyA signal"  
     misc_feature    5122..5141 
                     /label="Restriction Enzyme"  
     misc_feature    5926..6598 
                     /label="ColE1"  
     misc_feature    7028..7822 
                     /label="Kan/Neo"  
     misc_feature    7857..8159 
                     /label="SV40 ori"  
     misc_feature    8245..8722 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4926..4932 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4926 
                     /label="NEDD4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCACAAAGC TTACGATTGC ACTTTGCAGC CAGAAGAAGC AATACTTACC 
     1081 CTTTGTCAGA AACCTCCGGA GATGACTTGG ATAGCCATGT TCACATGTGC TTCAAAAGAC 
     1141 CAACACGGAT TTCAACGTCT AACGTTGTTC AAATGAAGCT GACTCCCAGA CAGACTGCAC 
     1201 TAGCTCCGTT AATAAAGGAA AACGTTCAGT CTCAAGAAAG ATCATCTGTT CCCTCATCTG 
     1261 AAAATGTTAA TAAAAAGAGC AGCTGTCTAC AGATTTCACT ACAGCCAACA AGGTACAGTG 
     1321 GATATCTTCA GTCTAGCAAT GTCTTAGCTG ATAGTGATGA TGCTTCGTTT ACTTGTATCT 
     1381 TGAAGGATGG TATTTACAGT AGTGCTGTGG TCGATAATGA ATTGAATGCT GTGAATGATG 
     1441 GTCACCTTGT AAGCAGTCCA GCCATTTGTA GTGGTAGCCT TAGTAACTTT TCAACCAGTG 
     1501 ATAATGGGTC TTACAGCAGC AACGGTAGTG ATTTTGGGTC ATGTGCAAGT ATCACAAGTG 
     1561 GAGGTTCATA TACTAACAGT GTCATCAGTG ACAGTAGTAG TTATACTTTT CCACCAAGTG 
     1621 ATGATACTTT TTTGGGTGGA AACTTACCTT CTGACAGCAC CTCCAATAGA AGTGTGCCAA 
     1681 ACAGGAATAC TACTCCTTGT GAAATTTTTT CAAGAAGTAC AAGTACAGAT CCTTTTGTCC 
     1741 AGGATGACTT GGAACATGGA TTAGAGATTA TGAAATTGCC AGTGAGCAGG AACACAAAAA 
     1801 TTCCACTAAA ACGTTACTCC TCCTTAGTCA TTTTTCCTAG GAGTCCTTCA ACTACCCGAC 
     1861 CGACTTCTCC AACAAGTCTG TGTACTCTTC TGAGCAAAGG ATCCTATCAA ACTTCACACC 
     1921 AGTTTATTAT TTCTCCTAGT GAAATTGCAC ATAATGAGGA TGGCACTAGT GCTAAAGGAT 
     1981 TTCTTTCAAC AGCTGTCAAT GGACTTCGGT TATCTAAAAC AATTTGTACC CCCGGAGAAG 
     2041 TAAGAGACAT ACGGCCGCTT CACAGGAAGG GCTCGTTACA GAAGAAAATT GTTCTTTCGA 
     2101 ATAATACTCC CAGACAGACT GTCTGTGAAA AGTCATCTGA AGGATATTCT TGTGTTTCAG 
     2161 TGCATTTCAC CCAACGAAAA GCAGCTACAT TAGACTGTGA AACAACAAAT GGTGATTGTA 
     2221 AACCAGAAAT GTCAGAAATT AAGCTTAATT CTGATTCAGA GTATATTAAG CTCATGCATA 
     2281 GGACATCTGC ATGTTTGCCA TCCTCCCAAA ATGTAGATTG TCAAATAAAT ATCAATGGAG 
     2341 AATTGGAAAG ACCACATTCA CAGATGAACA AAAACCATGG TATTTTACGA AGAAGTATTT 
     2401 CATTGGGAGG AGCTTATCCA AATATTTCCT GTCTATCCAG CCTTAAGCAC AATTGTTCTA 
     2461 AAGGGGGACC ATCTCAGTTA CTCATAAAGT TTGCATCTGG AAATGAAGGT AAAGTGGATA 
     2521 ATTTATCAAG AGACAGCAAC AGAGATTGCA CAAATGAACT GTCTAATTCT TGCAAGACAG 
     2581 AAAATCCAAG ATTGGAGAGA CCATATACAT TTAAGGATTT TGTTCTTCAT CCAAGAAGTC 
     2641 ACAAATCAAG AGTTAAAGGT TATCTGAGAC TAAAAATGAC TTATTTACCT AAAACCAGTG 
     2701 GCTCAGAAGA TGATAATGCA GAACAGGCTG AGGAATTAGA GCCTGGCTGG GTTGTTTTGG 
     2761 ACCAACCAGA TGCTGCTTGC CATTTGCAGC AACAACAAGA ACCTTCTCCT CTACCTCCAG 
     2821 GGTGGGAAGA GAGGCAGGAT ATCCTTGGAA GGACCTATTA TGTAAACCAT GAATCTAGAA 
     2881 GAACACAGTG GAAAAGACCA ACCCCTCAGG ACAACCTAAC AGATGCTGAG AATGGCAACA 
     2941 TTCAACTGCA AGCACAACGT GCATTTACCA CCAGGCGGCA GATATCCGAG GAAACAGAAA 
     3001 GTGTTGACAA CCGAGAGTCT TCCGAGAACT GGGAAATTAT AAGAGAAGAT GAAGCCACCA 
     3061 TGTATAGCAA CCAGGCCTTC CCATCACCTC CACCGTCAAG TAACTTGGAT GTTCCAACTC 
     3121 ATCTTGCAGA AGAATTGAAT GCCAGACTCA CCATTTTTGG AAATTCAGCC GTGAGCCAGC 
     3181 CAGCATCGAG CTCAAATCAT TCCAGCAGAA GAGGCAGCTT ACAAGCCTAT ACTTTTGAGG 
     3241 AACAACCTAC ACTTCCTGTG CTTTTGCCTA CTTCATCTGG ATTACCACCA GGTTGGGAAG 
     3301 AAAAACAAGA TGAAAGAGGA AGATCATATT ATGTAGATCA CAATTCCAGA ACGACTACTT 
     3361 GGACAAAGCC CACTGTACAG GCCACAGTGG AGACCAGTCA GCTGACCTCA AGCCAGAGTT 
     3421 CTGCAGGCCC TCAATCACAA GCCTCCACCA GTGATTCAGG CCAGCAGGTG ACCCAGCCAT 
     3481 CTGAAATTGA GCAAGGATTC CTTCCTAAAG GCTGGGAAGT CCGGCATGCA CCAAATGGGA 
     3541 GGCCTTTCTT TATTGACCAC AACACTAAAA CCACCACCTG GGAAGATCCA AGATTGAAAA 
     3601 TTCCAGCCCA TCTGAGAGGA AAGACATCAC TTGATACTTC CAATGATCTA GGGCCTTTAC 
     3661 CTCCAGGATG GGAAGAGAGA ACTCACACAG ATGGAAGAAT CTTCTACATA AATCACAATA 
     3721 TAAAAAGAAC ACAATGGGAA GATCCTCGGT TGGAGAATGT AGCAATAACT GGACCAGCAG 
     3781 TGCCCTACTC CAGGGATTAC AAAAGAAAGT ATGAGTTCTT CCGAAGAAAG TTGAAGAAGC 
     3841 AGAATGACAT TCCAAACAAA TTTGAAATGA AACTTCGCCG AGCAACTGTT CTTGAAGACT 
     3901 CTTACCGGAG AATTATGGGT GTCAAGAGAG CAGACTTCCT GAAGGCTCGA CTGTGGATTG 
     3961 AGTTTGATGG TGAAAAGGGA TTGGATTATG GAGGAGTTGC CAGAGAATGG TTCTTCCTGA 
     4021 TCTCAAAGGA AATGTTTAAC CCTTATTATG GGTTGTTTGA ATATTCTGCT ACGGACAATT 
     4081 ATACCCTACA GATAAATCCA AACTCTGGAT TGTGTAACGA AGATCACCTC TCTTACTTCA 
     4141 AGTTTATTGG TCGGGTAGCT GGAATGGCAG TTTATCATGG CAAACTGTTG GATGGTTTTT 
     4201 TCATCCGCCC ATTTTACAAG ATGATGCTTC ACAAACCAAT AACCCTTCAT GATATGGAAT 
     4261 CTGTGGATAG TGAATATTAC AATTCCCTAA GATGGATTCT TGAAAATGAC CCAACAGAAT 
     4321 TGGACCTCAG GTTTATCATA GATGAAGAAC TTTTTGGACA GACACATCAA CATGAGCTGA 
     4381 AAAATGGTGG ATCAGAAATA GTTGTCACCA ATAAGAACAA AAAGGAATAT ATTTATCTTG 
     4441 TAATACAATG GCGATTTGTA AACCGAATCC AGAAGCAAAT GGCTGCTTTT AAAGAGGGAT 
     4501 TCTTTGAACT AATACCACAG GATCTCATCA AAATTTTTGA TGAAAATGAA CTAGAGCTTC 
     4561 TTATGTGTGG ACTGGGAGAT GTTGATGTGA ATGACTGGAG GGAACATACA AAGTATAAAA 
     4621 ATGGCTACAG TGCAAATCAT CAGGTTATAC AGTGGTTTTG GAAGGCTGTT TTAATGATGG 
     4681 ATTCAGAAAA AAGAATAAGA TTACTTCAGT TTGTCACTGG CACATCTCGG GTGCCTATGA 
     4741 ATGGATTTGC TGAACTATAC GGTTCAAATG GACCACAGTC ATTTACAGTT GAACAGTGGG 
     4801 GTACTCCTGA AAAGCTGCCA AGAGCTCATA CCTGTTTTAA TCGCCTGGAC TTGCCACCTT 
     4861 ATGAATCATT TGAAGAATTA TGGGATAAAC TTCAGATGGC AATTGAAAAC ACCCAGGGCT 
     4921 TTGATGGAGT TGATACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4981 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     5041 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     5101 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     5161 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     5221 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5281 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5341 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5401 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5461 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5521 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5581 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5641 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5701 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5761 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5821 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5881 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5941 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     6001 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     6061 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     6121 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     6181 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6241 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6301 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6361 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6421 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6481 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6541 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6601 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6661 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6721 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6781 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6841 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6901 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6961 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     7021 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     7081 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     7141 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     7201 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7261 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7321 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7381 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7441 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7501 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7561 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7621 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7681 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7741 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7801 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7861 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7921 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7981 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     8041 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8101 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8161 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     8221 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8281 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8341 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8401 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8461 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8521 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8581 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8641 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8701 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8761 ATTTAACAAA AATTTAACGC GAATTTT
//