LOCUS       RC240099                8775 bp    DNA     circular  SYN 
DEFINITION  SEC24B (myc-DDK-tagged) - Human SEC24 family member B (SEC24B), transcript variant 3
ACCESSION   NM_001300813
VERSION     NM_001300813.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4932..5023 
                     /label="Myc-DDK"  
     misc_feature    5053..5638 
                     /label="PolyA signal"  
     misc_feature    5110..5129 
                     /label="Restriction Enzyme"  
     misc_feature    5914..6586 
                     /label="ColE1"  
     misc_feature    7016..7810 
                     /label="Kan/Neo"  
     misc_feature    7845..8147 
                     /label="SV40 ori"  
     misc_feature    8233..8710 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4914..4920 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4914 
                     /label="SEC24B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGGCCCCC GCCGGGTCCT CTCACCCGGC CGCCAGCGCC CGGATCCCGC 
     1081 CCAAGTTCGG CGGAGCGGCC GTCTCAGGAG CCGCAGCGCC CGCGGGCCCG GGTGCGGGCC 
     1141 CGGCGCCGCA CCAGCAGAAC GAGACGGGAT TTCACCATGT TGCCCAAGCT AGTCTTGAAC 
     1201 TCCTGGACCC AAGCAATCTG CCCGCCTCGG CCTCCCAAAT TGCTGGGAGT ACAGGTCCAG 
     1261 CCCAGAATCA AATGCAGGTT CCATCTGGAT ATGGATTGCA TCATCAAAAC TATATTGCTC 
     1321 CCTCAGGACA TTACTCTCAA GGACCTGGGA AAATGACCTC ATTGCCATTG GATACCCAGT 
     1381 GTGGTGATTA CTACTCTGCT CTCTATACAG TACCAACACA AAATGTGACT CCTAACACAG 
     1441 TGAACCAGCA ACCAGGAGCA CAGCAGTTGT ACAGCAGGGG TCCTCCTGCC CCTCATATTG 
     1501 TGGGATCCAC TCTAGGATCT TTCCAAGGTG CTGCATCGTC AGCATCCCAT TTGCATACGA 
     1561 GTGCCTCCCA ACCATACTCC TCTTTTGTGA ATCACTACAA TAGTCCAGCC ATGTACTCTG 
     1621 CCAGCTCTTC TGTTGCGTCT CAGGGATTTC CCTCTACTTG TGGTCATTAT GCTATGTCAA 
     1681 CTGTTTCTAA TGCCGCGTAT CCTAGTGTTT CATATCCCTC TCTGCCTGCT GGTGATACAT 
     1741 ATGGGCAAAT GTTTACCTCA CAGAATGCTC CGACTGTTAG GCCAGTTAAA GATAATTCAT 
     1801 TCTCTGGTCA AAATACAGCT ATCAGCCATC CATCGCCACT TCCACCTCTA CCATCACAAC 
     1861 AGCACCACCA GCAGCAAAGT CTTTCAGGAT ACAGTACTCT AACGTGGTCA TCTCCAGGCC 
     1921 TTCCATCGAC TCAAGACAAT CTCATCCGAA ACCACACAGG ATCCCTGGCT GTAGCGAACA 
     1981 ACAACCCAAC CATTACTGTT GCAGATTCTT TATCCTGTCC TGTTATGCAA AATGTTCAGC 
     2041 CTCCCAAGTC CAGCCCAGTG GTATCCACTG TTTTATCAGG ATCCTCAGGA TCCTCATCAA 
     2101 CAAGAACACC TCCCACTGCA AATCACCCAG TTGAGCCTGT GACCTCAGTT ACACAGCCAT 
     2161 CAGAGCTATT ACAACAAAAA GGCGTGCAGT ATGGTGAATA TGTTAATAAC CAAGCTAGCT 
     2221 CCGCACCAAC TCCCTTGTCA TCAACTTCCG ATGATGAGGA AGAGGAGGAG GAGGATGAGG 
     2281 AAGCAGGTGT TGACAGCTCT TCTACCACAA GCAGTGCTTC TCCAATGCCC AACAGTTATG 
     2341 ATGCCCTGGA AGGAGGCAGT TACCCAGATA TGCTTTCTTC ATCAGCAAGC AGTCCTGCTC 
     2401 CTGATCCCGC CCCTGAACCT GATCCTGCTT CTGCTCCAGC TCCAGCTTCA GCTCCAGCTC 
     2461 CTGTCGTCCC TCAGCCTTCA AAAATGGCTA AGCCTTTTGG CTATGGCTAT CCAACACTTC 
     2521 AGCCTGGTTA TCAGAATGCT ACAGCACCAC TTATTTCTGG AGTACAGCCC AGTAACCCGG 
     2581 TATATTCTGG ATTCCAGCAG TATCCTCAAT ATCCTGGTGT GAACCAGCTA TCCTCCAGTA 
     2641 TAGGAGGATT GAGTCTTCAG AGTTCTCCAC AACCAGAAAG CCTGAGACCT GTAAACCTTA 
     2701 CTCAGGAGAG GAATATTTTA CCTATGACTC CTGTTTGGGC TCCTGTACCT AACTTGAATG 
     2761 CAGACCTCAA AAAATTAAAC TGTAGCCCAG ATTCATTTCG GTGTACTTTG ACAAATATTC 
     2821 CACAGACACA GGCTTTACTG AATAAAGCTA AGCTTCCTTT AGGATTGTTG TTACATCCCT 
     2881 TCAGAGACCT AACGCAATTA CCAGTGATAA CATCAAATAC CATTGTGAGG TGCCGATCCT 
     2941 GTCGAACGTA TATTAACCCC TTTGTATCCT TCATTGATCA ACGTAGATGG AAATGCAATT 
     3001 TGTGCTATAG AGTAAACGAT GTTCCTGAAG AATTTATGTA TAACCCCCTT ACCCGATCTT 
     3061 ATGGAGAGCC TCATAAACGA CCAGAAGTTC AGAATTCAAC TGTGGAGTTC ATTGCTTCTT 
     3121 CAGATTACAT GCTGCGTCCT CCTCAACCTG CAGTTTACTT GTTTGTTTTA GATGTGTCTC 
     3181 ATAATGCAGT GGAAGCTGGA TATTTGACAA TTTTGTGCCA GTCACTCCTA GAAAATCTAG 
     3241 ACAAGCTTCC TGGAGATTCA CGAACAAGAA TAGGATTCAT GACCTTTGAT AGCACTATTC 
     3301 ATTTCTACAA TTTACAAGAA GGATTATCAC AGCCTCAAAT GTTGATTGTG TCTGATATAG 
     3361 ATGATGTTTT TCTACCTACA CCGGATAGTT TACTTGTGAA TCTATATGAA AGTAAAGAGC 
     3421 TTATAAAAGA CTTACTGAAT GCATTACCAA ACATGTTCAC CAATACAAGA GAAACACACA 
     3481 GTGCCCTTGG TCCTGCACTT CAGGCTGCCT TTAAATTAAT GTCTCCAACA GGTGGCCGTG 
     3541 TGTCTGTATT TCAGACACAG TTACCTTCCT TGGGTGCAGG ACTTCTGCAA TCCAGAGAAG 
     3601 ATCCTAATCA GAGATCAAGT ACAAAGGTGG TACAACATCT TGGCCCTGCA ACTGATTTTT 
     3661 ATAAGAAACT TGCATTAGAT TGCTCGGGAC AGCAAACTGC AGTGGATTTG TTCCTTTTAA 
     3721 GTTCACAGTA TTCTGATCTT GCTTCTCTAG CTTGCATGTC CAAGTATTCT GCAGGGTGCA 
     3781 TCTATTATTA TCCATCATTC CACTATACTC ACAATCCTTC ACAAGCAGAA AAGTTACAAA 
     3841 AAGACCTAAA ACGGTATCTC ACAAGAAAAA TTGGGTTTGA AGCTGTTATG AGAATAAGGT 
     3901 GTACTAAAGG TCTTTCAATG CACACTTTTC ACGGTAACTT CTTTGTCCGT TCTACTGATT 
     3961 TGTTATCCCT TGCCAACATC AATCCTGATG CTGGATTTGC GGTGCAGTTG TCAATTGAAG 
     4021 AAAGTTTAAC AGATACTTCC TTAGTATGTT TTCAAACAGC CCTATTATAT ACATCAAGCA 
     4081 AAGGTGAGCG GAGAATTAGA GTACATACAC TTTGTTTGCC AGTGGTAAGT TCACTAGCAG 
     4141 ATGTATATGC GGGAGTGGAT GTACAAGCTG CCATCTGCCT TCTGGCAAAC ATGGCTGTGG 
     4201 ATCGGTCCGT TTCATCAAGT CTGTCAGATG CAAGAGATGC CTTAGTGAAT GCTGTAGTGG 
     4261 ACTCATTGTC TGCATATGGC TCAACTGTCT CAAATTTACA GCACTCTGCA TTGATGGCGC 
     4321 CCAGCTCCCT CAAGTTGTTT CCTCTCTATG TTTTGGCCCT TCTCAAACAG AAAGCATTTA 
     4381 GAACGGGTAC AAGCACACGG CTGGATGATC GTGTATATGC CATGTGTCAG ATAAAGTCTC 
     4441 AGCCACTTGT TCATCTAATG AAAATGATTC ATCCCAACTT ATACAGGATA GACAGATTGA 
     4501 CAGATGAGGG TGCAGTACAT GTTAATGACA GGATTGTACC ACAGCCACCT CTTCAAAAAT 
     4561 TGTCTGCAGA GAAGCTGACA AGAGAAGGTG CTTTCCTTAT GGACTGTGGC TCTGTTTTTT 
     4621 ACATTTGGGT TGGGAAAGGC TGTGACAATA ACTTCATAGA GGATGTGCTT GGATATACTA 
     4681 ATTTTGCATC AATACCACAG AAAATGACAC ATCTTCCAGA GCTAGATACA CTTTCATCAG 
     4741 AAAGAGCCAG ATCCTTCATA ACTTGGCTTA GAGACAGCAG ACCATTAAGT CCAATCCTTC 
     4801 ACATAGTAAA AGATGAGAGT CCTGCCAAAG CAGAATTTTT TCAGCATTTG ATTGAAGACC 
     4861 GGACAGAGGC TGCATTTTCT TACTATGAAT TTTTGCTTCA TGTTCAGCAG CAGATTTGTA 
     4921 AGACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4981 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     5041 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     5101 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     5161 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     5221 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5281 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5341 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5401 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5461 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5521 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5581 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5641 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5701 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5761 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5821 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5881 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5941 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     6001 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     6061 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     6121 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     6181 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6241 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6301 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6361 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6421 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6481 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6541 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6601 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6661 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6721 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6781 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6841 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6901 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6961 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     7021 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     7081 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     7141 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     7201 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7261 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7321 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7381 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7441 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7501 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7561 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7621 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7681 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7741 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7801 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7861 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7921 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7981 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     8041 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     8101 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8161 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     8221 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8281 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8341 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8401 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8461 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8521 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8581 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8641 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8701 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8761 TTTAACGCGA ATTTT
//