LOCUS       RC240098                8751 bp    DNA     circular  SYN 
DEFINITION  TBC1D4 (myc-DDK-tagged) - Human TBC1 domain family, member 4 (TBC1D4), transcript variant 2
ACCESSION   NM_001286658
VERSION     NM_001286658.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4908..4999 
                     /label="Myc-DDK"  
     misc_feature    5029..5614 
                     /label="PolyA signal"  
     misc_feature    5086..5105 
                     /label="Restriction Enzyme"  
     misc_feature    5890..6562 
                     /label="ColE1"  
     misc_feature    6992..7786 
                     /label="Kan/Neo"  
     misc_feature    7821..8123 
                     /label="SV40 ori"  
     misc_feature    8209..8686 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4890..4896 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4890 
                     /label="TBC1D4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCCGCCC AGCTGCATTC AGGATGAGCC GTTCCCGCAC CCCCTGGAGC 
     1081 CCGAGCCGGG CGTCTCAGCT CAGCCCGGCC CCGGGAAGCC AAGCGATAAG CGGTTCCGGC 
     1141 TGTGGTACGT TGGGGGGTCG TGCCTGGACC ACAGGACCAC GCTGCCTATG CTGCCCTGGC 
     1201 TCATGGCCGA GATCCGCAGG CGCAGCCAGA AGCCCGAGGC GGGCGGCTGC GGGGCGCCGG 
     1261 CGGCCCGAGA GGTGATCCTG GTGCTCAGCG CGCCCTTCCT GCGTTGCGTC CCCGCGCCGG 
     1321 GCGCTGGGGC CTCGGGGGGC ACTAGTCCGT CGGCCACGCA GCCCAACCCG GCGGTATTCA 
     1381 TCTTCGAGCA CAAGGCGCAG CATATCTCGC GCTTCATCCA CAACAGCCAC GACCTCACCT 
     1441 ACTTTGCCTA CCTGATCAAG GCGCAGCCCG ACGACCCCGA GTCGCAGATG GCCTGCCACG 
     1501 TTTTCCGCGC CACAGACCCC AGCCAGGTTC CTGATGTTAT TAGCAGCATA AGGCAATTAT 
     1561 CTAAAGCGGC CATGAAAGAG GATGCCAAAC CCAGCAAAGA TAATGAGGAC GCCTTTTACA 
     1621 ACTCTCAGAA GTTCGAAGTC CTGTACTGTG GAAAGGTGAC CGTGACCCAC AAGAAGGCCC 
     1681 CCTCAAGCCT CATCGATGAC TGCATGGAGA AGTTCAGCCT GCACGAACAG CAGCGCCTGA 
     1741 AGATCCAAGG GGAGCAGCGC GGTCCGGACC CAGGAGAGGA CCTGGCTGAC TTGGAGGTGG 
     1801 TGGTGCCCGG GTCCCCCGGA GACTGCCTGC CGGAGGAGGC TGACGGCACC GACACCCACC 
     1861 TTGGCTTACC TGCCGGGGCC AGCCAGCCTG CCCTGACCAG CTCTCGGGTC TGCTTCCCTG 
     1921 AGCGGATTTT GGAAGATTCT GGCTTTGATG AGCAGCAGGA GTTTCGGTCT CGGTGCAGCA 
     1981 GTGTCACCGG CGTGCAACGG AGAGTTCACG AGGGCAGCCA GAAATCCCAG CCGCGACGGA 
     2041 GACACGCGAG CGCACCCAGT CACGTCCAGC CCTCGGACTC GGAGAAGAAC AGGACCATGC 
     2101 TCTTCCAGGT TGGGCGATTT GAGATTAACC TTATCAGTCC AGACACTAAA TCAGTTGTGC 
     2161 TAGAAAAGAA TTTTAAAGAT ATCTCCTCTT GTTCTCAGGG TATAAAGCAT GTGGATCACT 
     2221 TTGGCTTTAT CTGCCGGGAG TCTCCAGAGC CTGGACTTAG CCAGTATATT TGTTATGTAT 
     2281 TCCAGTGTGC CAGCGAATCT CTGGTTGATG AGGTAATGCT GACTCTGAAA CAGGCCTTCA 
     2341 GTACGGCGGC TGCCCTGCAG AGTGCCAAGA CGCAGATTAA ACTGTGTGAG GCCTGCCCGA 
     2401 TGCACTCTTT GCATAAGCTC TGTGAAAGGA TTGAAGGTCT CTACCCACCA AGAGCCAAGC 
     2461 TGGTGATACA GAGGCATCTC TCATCACTGA CAGATAATGA GCAAGCTGAC ATCTTTGAAA 
     2521 GAGTTCAGAA AATGAAGCCA GTCAGTGACC AGGAAGAAAA TGAACTTGTG ATTTTACACC 
     2581 TGAGGCAGCT GTGTGAAGCC AAGCAGAAGA CACACGTGCA CATCGGGGAA GGCCCTTCTA 
     2641 CTATTTCAAA TAGTACAATC CCAGAAAATG CAACAAGCAG TGGAAGGTTC AAACTTGACA 
     2701 TTCTGAAAAA TAAAGCTAAG AGATCCTTAA CTAGCTCCCT GGAAAATATC TTCTCAAGGG 
     2761 GAGCTAACAG AATGAGAGGT CGGCTTGGAA GTGTGGACAG TTTTGAACGG TCCAACAGTC 
     2821 TTGCTTCAGA GAAGGACTAC TCACCAGGGG ATTCTCCACC AGGGACACCG CCAGCGTCCC 
     2881 CACCGTCCTC AGCTTGGCAA ACGTTTCCCG AAGAGGATTC CGACTCCCCG CAGTTTCGAA 
     2941 GACGGGCACA CACGTTCAGC CACCCACCTT CAAGCACAAA GAGAAAGCTG AATTTGCAGG 
     3001 ATGGGAGGGC TCAGGGTGTG CGTTCCCCTC TGCTGAGGCA GAGCTCCAGT GAACAGTGCA 
     3061 GCAATCTTTC GTCAGTTCGA CGCATGTACA AGGAGAGTAA TTCTTCCTCC AGTCTTCCAA 
     3121 GTCTTCACAC TTCCTTCTCT GCCCCTTCCT TCACTGCCCC CTCTTTCCTG AAAAGCTTTT 
     3181 ACCAGAATTC AGGTAGACTG TCCCCACAGT ATGAAAATGA AATCAGTGAT GGAGAAGGGA 
     3241 GAAAAAGGAC CTCATCTACC TGCAGCAATG AGTCCCTAAG TGTGGGAGGA ACCTCTGTCA 
     3301 CTCCTCGCCG GATCTCCTGG CGGCAGCGCA TTTTCCTCAG GGTTGCTTCT CCCATGAACA 
     3361 AATCTCCCTC AGCAATGCAA CAGCAAGATG GATTGGACAG GAACGAGCTG CTGCCACTGT 
     3421 CCCCCCTCTC TCCAACCATG GAGGAGGAAC CGCTGGTTGT ATTCCTGTCT GGGGAGGATG 
     3481 ACCCAGAAAA GATTGAAGAA AGAAAGAAAT CAAAAGAACT GAGGAGCTTG TGGAGAAAAG 
     3541 CTATACACCA ACAAATCTTG TTACTTCGAA TGGAAAAAGA AAACCAGAAA CTTGAAGCAA 
     3601 GCAGAGATGA ACTCCAGTCC AGAAAAGTTA AATTAGACTA TGAAGAAGTT GGTGCATGTC 
     3661 AGAAAGAGGT CTTAATAACT TGGGATAAGA AGTTGTTAAA CTGCAGAGCT AAAATCAGAT 
     3721 GTGATATGGA AGATATTCAT ACTCTTCTTA AAGAAGGAGT TCCCAAAAGT CGACGAGGAG 
     3781 AAATTTGGCA GTTTCTGGCT TTACAGTACC GACTCAGACA CAGATTGCCT AATAAACAAC 
     3841 AGCCTCCTGA CATATCCTAT AAGGAACTTT TGAAGCAGCT CACTGCTCAG CAGCATGCGA 
     3901 TTCTCGTGGA TTTAGGAAGG ACGTTTCCTA CTCACCCTTA CTTTTCAGTA CAGCTTGGGC 
     3961 CAGGACAGCT GTCACTGTTT AACCTCCTGA AAGCCTATTC TTTGCTGGAC AAAGAAGTGG 
     4021 GATACTGTCA GGGGATCAGC TTTGTGGCTG GAGTCCTGCT TCTGCACATG AGTGAAGAGC 
     4081 AAGCCTTTGA AATGCTGAAA TTCCTCATGT ATGACCTCGG CTTCCGCAAG CAGTACAGAC 
     4141 CTGACATGAT GTCGCTGCAG ATTCAAATGT ACCAGCTGTC CAGGCTCCTT CATGACTATC 
     4201 ACAGAGATCT CTACAATCAC CTTGAAGAAA ATGAAATCAG CCCCAGTCTT TATGCTGCCC 
     4261 CCTGGTTCCT CACATTGTTT GCCTCTCAGT TTTCATTAGG ATTTGTAGCC AGAGTTTTTG 
     4321 ATATTATTTT TCTTCAGGGA ACTGAAGTTA TATTCAAGGT TGCACTCAGC CTACTGAGCA 
     4381 GCCAAGAGAC ACTTATAATG GAATGTGAGA GCTTTGAAAA TATTGTTGAG TTTCTTAAAA 
     4441 ACACGCTACC TGATATGAAT ACCTCTGAAA TGGAAAAAAT TATTACCCAG GTTTTTGAGA 
     4501 TGGATATTTC TAAGCAGTTG CATGCCTATG AGGTGGAATA TCATGTGCTA CAGGATGAGC 
     4561 TTCAGGAATC TTCATATTCC TGTGAGGATA GTGAAACTTT GGAGAAGCTG GAGAGGGCCA 
     4621 ATAGCCAACT GAAAAGACAA AACATGGACC TCCTAGAAAA ATTACAGGTA GCTCATACTA 
     4681 AAATCCAGGC CTTGGAATCA AACCTGGAAA ATCTTTTGAC GAGAGAGACC AAAATGAAGT 
     4741 CTTTAATCCG GACCCTGGAA CAAGAAAAAA TGGCTTATCA AAAGACAGTG GAGCAACTCC 
     4801 GGAAGCTGCT GCCCGCGGAT GCTCTAGTCA ATTGTGACCT GTTGCTGAGA GACCTAAACT 
     4861 GCAACCCTAA CAACAAAGCC AAGATAGGAA ATAAGCCAAC GCGTACGCGG CCGCTCGAGC 
     4921 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4981 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     5041 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     5101 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     5161 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     5221 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5281 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5341 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5401 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5461 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5521 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5581 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5641 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5701 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5761 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5821 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5881 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5941 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     6001 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     6061 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     6121 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     6181 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6241 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6301 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6361 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6421 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6481 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6541 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6601 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6661 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6721 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6781 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6841 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6901 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6961 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     7021 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     7081 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     7141 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     7201 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7261 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7321 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7381 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7441 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7501 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7561 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7621 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7681 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7741 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7801 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7861 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7921 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7981 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8041 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     8101 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     8161 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     8221 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8281 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8341 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8401 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8461 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8521 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8581 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8641 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8701 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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