LOCUS       RC240095                8739 bp    DNA     circular  SYN 
DEFINITION  BLM (myc-DDK-tagged) - Human Bloom syndrome, RecQ helicase-like (BLM), transcript variant 3
ACCESSION   NM_001287247
VERSION     NM_001287247.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4896..4987 
                     /label="Myc-DDK"  
     misc_feature    5017..5602 
                     /label="PolyA signal"  
     misc_feature    5074..5093 
                     /label="Restriction Enzyme"  
     misc_feature    5878..6550 
                     /label="ColE1"  
     misc_feature    6980..7774 
                     /label="Kan/Neo"  
     misc_feature    7809..8111 
                     /label="SV40 ori"  
     misc_feature    8197..8674 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4878..4884 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4878 
                     /label="BLM"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGCTGTT CCTCAAAATA ATCTACAGGA GCAACTAGAA CGTCACTCAG 
     1081 CCAGAACACT TAATAATAAA TTAAGTCTTT CAAAACCAAA ATTTTCAGGT TTCACTTTTA 
     1141 AAAAGAAAAC ATCTTCAGAT AACAATGTAT CTGTAACTAA TGTGTCAGTA GCAAAAACAC 
     1201 CTGTATTAAG AAATAAAGAT GTTAATGTTA CCGAAGACTT TTCCTTCAGT GAACCTCTAC 
     1261 CCAACACCAC AAATCAGCAA AGGGTCAAGG ACTTCTTTAA AAATGCTCCA GCAGGACAGG 
     1321 AAACACAGAG AGGTGGATCA AAATCATTAT TGCCAGATTT CTTGCAGACT CCGAAGGAAG 
     1381 TTGTATGCAC TACCCAAAAC ACACCAACTG TAAAGAAATC CCGGGATACT GCTCTCAAGA 
     1441 AATTAGAATT TAGTTCTTCA CCAGATTCTT TAAGTACCAT CAATGATTGG GATGATATGG 
     1501 ATGACTTTGA TACTTCTGAG ACTTCAAAAT CATTTGTTAC ACCACCCCAA AGTCACTTTG 
     1561 TAAGAGTAAG CACTGCTCAG AAATCAAAAA AGGGTAAGAG AAACTTTTTT AAAGCACAGC 
     1621 TTTATACAAC AAACACAGTA AAGACTGATT TGCCTCCACC CTCCTCTGAA AGCGAGCAAA 
     1681 TAGATTTGAC TGAGGAACAG AAGGATGACT CAGAATGGTT AAGCAGCGAT GTGATTTGCA 
     1741 TCGATGATGG CCCCATTGCT GAAGTGCATA TAAATGAAGA TGCTCAGGAA AGTGACTCTC 
     1801 TGAAAACTCA TTTGGAAGAT GAAAGAGATA ATAGCGAAAA GAAGAAGAAT TTGGAAGAAG 
     1861 CTGAATTACA TTCAACTGAG AAAGTTCCAT GTATTGAATT TGATGATGAT GATTATGATA 
     1921 CGGATTTTGT TCCACCTTCT CCAGAAGAAA TTATTTCTGC TTCTTCTTCC TCTTCAAAAT 
     1981 GCCTTAGTAC GTTAAAGGAC CTTGACACCT CTGACAGAAA AGAGGATGTT CTTAGCACAT 
     2041 CAAAAGATCT TTTGTCAAAA CCTGAGAAAA TGAGTATGCA GGAGCTGAAT CCAGAAACCA 
     2101 GCACAGACTG TGACGCTAGA CAGATAAGTT TACAGCAGCA GCTTATTCAT GTGATGGAGC 
     2161 ACATCTGTAA ATTAATTGAT ACTATTCCTG ATGATAAACT GAAACTTTTG GATTGTGGGA 
     2221 ACGAACTGCT TCAGCAGCGG AACATAAGAA GGAAACTTCT AACGGAAGTA GATTTTAATA 
     2281 AAAGTGATGC CAGTCTTCTT GGCTCATTGT GGAGATACAG GCCTGATTCA CTTGATGGCC 
     2341 CTATGGAGGG TGATTCCTGC CCTACAGGGA ATTCTATGAA GGAGTTAAAT TTTTCACACC 
     2401 TTCCCTCAAA TTCTGTTTCT CCTGGGGACT GTTTACTGAC TACCACCCTA GGAAAGACAG 
     2461 GATTCTCTGC CACCAGGAAG AATCTTTTTG AAAGGCCTTT ATTCAATACC CATTTACAGA 
     2521 AGTCCTTTGT AAGTAGCAAC TGGGCTGAAA CACCAAGACT AGGAAAAAAA AATGAAAGCT 
     2581 CTTATTTCCC AGGAAATGTT CTCACAAGCA CTGCTGTGAA AGATCAGAAT AAACATACTG 
     2641 CTTCAATAAA TGACTTAGAA AGAGAAACCC AACCTTCCTA TGATATTGAT AATTTTGACA 
     2701 TAGATGACTT TGATGATGAT GATGACTGGG AAGACATAAT GCATAATTTA GCAGCCAGCA 
     2761 AATCTTCCAC AGCTGCCTAT CAACCCATCA AGGAAGGTCG GCCAATTAAA TCAGTATCAG 
     2821 AAAGACTTTC CTCAGCCAAG ACAGACTGTC TTCCAGTGTC ATCTACTGCT CAAAATATAA 
     2881 ACTTCTCAGA GTCAATTCAG AATTATACTG ACAAGTCAGC ACAAAATTTA GCATCCAGAA 
     2941 ATCTGAAACA TGAGCGTTTC CAAAGTCTTA GTTTTCCTCA TACAAAGGAA ATGATGAAGA 
     3001 TTTTTCATAA AAAATTTGGC CTGCATAATT TTAGAACTAA TCAGCTAGAG GCGATCAATG 
     3061 CTGCACTGCT TGGTGAAGAC TGTTTTATCC TGATGCCGAC TGGAGGTGGT AAGAGTTTGT 
     3121 GTTACCAGCT CCCTGCCTGT GTTTCTCCTG GGGTCACTGT TGTCATTTCT CCCTTGAGAT 
     3181 CACTTATCGT AGATCAAGTC CAAAAGCTGA CTTCCTTGGA TATTCCAGCT ACATATCTGA 
     3241 CAGGTGATAA GACTGACTCA GAAGCTACAA ATATTTACCT CCAGTTATCA AAAAAAGACC 
     3301 CAATCATAAA ACTTCTATAT GTCACTCCAG AAAAGATCTG TGCAAGTAAC AGACTCATTT 
     3361 CTACTCTGGA GAATCTCTAT GAGAGGAAGC TCTTGGCACG TTTTGTTATT GATGAAGCAC 
     3421 ATTGTGTCAG TCAGTGGGGA CATGATTTTC GTCAAGATTA CAAAAGAATG AATATGCTTC 
     3481 GCCAGAAGTT TCCTTCTGTT CCGGTGATGG CTCTTACGGC CACAGCTAAT CCCAGGGTAC 
     3541 AGAAGGACAT CCTGACTCAG CTGAAGATTC TCAGACCTCA GGTGTTTAGC ATGAGCTTTA 
     3601 ACAGACATAA TCTGAAATAC TATGTATTAC CGAAAAAGCC TAAAAAGGTG GCATTTGATT 
     3661 GCCTAGAATG GATCAGAAAG CACCACCCAT ATGATTCAGG GATAATTTAC TGCCTCTCCA 
     3721 GGCGAGAATG TGACACCATG GCTGACACGT TACAGAGAGA TGGGCTCGCT GCTCTTGCTT 
     3781 ACCATGCTGG CCTCAGTGAT TCTGCCAGAG ATGAAGTGCA GCAGAAGTGG ATTAATCAGG 
     3841 ATGGCTGTCA GGTTATCTGT GCTACAATTG CATTTGGAAT GGGGATTGAC AAACCGGACG 
     3901 TGCGATTTGT GATTCATGCA TCTCTCCCTA AATCTGTGGA GGGTTACTAC CAAGAATCTG 
     3961 GCAGAGCTGG AAGAGATGGG GAAATATCTC ACTGCCTGCT TTTCTATACC TATCATGATG 
     4021 TGACCAGACT GAAAAGACTT ATAATGATGG AAAAAGATGG AAACCATCAT ACAAGAGAAA 
     4081 CTCACTTCAA TAATTTGTAT AGCATGGTAC ATTACTGTGA AAATATAACG GAATGCAGGA 
     4141 GAATACAGCT TTTGGCCTAC TTTGGTGAAA ATGGATTTAA TCCTGATTTT TGTAAGAAAC 
     4201 ACCCAGATGT TTCTTGTGAT AATTGCTGTA AAACAAAGGA TTATAAAACA AGAGATGTGA 
     4261 CTGACGATGT GAAAAGTATT GTAAGATTTG TTCAAGAACA TAGTTCATCA CAAGGAATGA 
     4321 GAAATATAAA ACATGTAGGT CCTTCTGGAA GATTTACTAT GAATATGCTG GTCGACATTT 
     4381 TCTTGGAATC TTTATCTTCT GATCCTGAGG TTTTGCTTCA AATTGATGGT GTTACTGAAG 
     4441 ACAAACTGGA AAAATATGGT GCGGAAGTGA TTTCAGTATT ACAGAAATAC TCTGAATGGA 
     4501 CATCGCCAGC TGAAGACAGT TCCCCAGGGA TAAGCCTGTC CAGCAGCAGA GGCCCCGGAA 
     4561 GAAGTGCCGC TGAGGAGCTC GACGAGGAAA TACCCGTATC TTCCCACTAC TTTGCAAGTA 
     4621 AAACCAGAAA TGAAAGGAAG AGGAAAAAGA TGCCAGCCTC CCAAAGGTCT AAGAGGAGAA 
     4681 AAACTGCTTC CAGTGGTTCC AAGGCAAAGG GGGGGTCTGC CACATGTAGA AAGATATCTT 
     4741 CCAAAACGAA ATCCTCCAGC ATCATTGGAT CCAGTTCAGC CTCACATACT TCTCAAGCGA 
     4801 CATCAGGAGC CAATAGCAAA TTGGGGATTA TGGCTCCACC GAAGCCTATA AATAGACCGT 
     4861 TTCTTAAGCC TTCATATGCA TTCTCAACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4921 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4981 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     5041 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     5101 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     5161 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     5221 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     5281 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5341 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5401 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5461 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5521 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5581 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5641 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5701 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5761 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5821 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5881 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5941 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     6001 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     6061 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     6121 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     6181 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     6241 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6301 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6361 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6421 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6481 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6541 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6601 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6661 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6721 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6781 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6841 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6901 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6961 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     7021 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     7081 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     7141 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     7201 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     7261 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7321 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7381 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7441 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7501 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7561 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7621 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7681 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7741 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7801 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7861 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7921 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7981 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8041 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8101 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     8161 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     8221 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     8281 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     8341 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8401 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8461 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8521 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8581 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8641 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8701 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//