LOCUS       RC240094                8745 bp    DNA     circular  SYN 
DEFINITION  TRPS1 (myc-DDK-tagged) - Human trichorhinophalangeal syndrome I (TRPS1), transcript variant 2
ACCESSION   NM_001282902
VERSION     NM_001282902.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4900..4991 
                     /label="Myc-DDK"  
     misc_feature    5021..5606 
                     /label="PolyA signal"  
     misc_feature    5078..5097 
                     /label="Restriction Enzyme"  
     misc_feature    5882..6554 
                     /label="ColE1"  
     misc_feature    6984..7778 
                     /label="Kan/Neo"  
     misc_feature    7813..8115 
                     /label="SV40 ori"  
     misc_feature    8201..8678 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4875..4882 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4875 
                     /label="TRPS1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGAGTAAT ATGGTCCGGA AAAAGAACCC CCCTCTGAGA AACGTTGCAA 
     1081 GTGAAGGCGA GGGCCAGATC CTGGAGCCTA TAGGTACAGA AAGCAAGGTA TCTGGAAAGA 
     1141 ACAAAGAATT TTCTGCAGAT CAGATGTCAG AAAATACGGA TCAGAGTGAT GCTGCAGAAC 
     1201 TAAATCATAA GGAGGAACAT AGCTTGCATG TTCAAGATCC ATCTTCTAGC AGTAAGAAGG 
     1261 ACTTGAAAAG CGCAGTTCTG AGTGAGAAGG CTGGCTTCAA TTATGAAAGC CCCAGTAAGG 
     1321 GAGGAAACTT TCCCTCCTTT CCGCATGATG AGGTGACAGA CAGAAATATG TTGGCTTTCT 
     1381 CATCTCCAGC TGCTGGGGGA GTCTGTGAGC CCTTGAAGTC TCCGCAAAGA GCAGAGGCAG 
     1441 ATGACCCTCA AGATATGGCC TGCACCCCCT CAGGGGACTC ACTGGAGACA AAGGAAGATC 
     1501 AGAAGATGTC ACCAAAGGCT ACAGAGGAAA CAGGGCAAGC ACAGAGTGGT CAAGCCAATT 
     1561 GTCAAGGTTT GAGCCCAGTT TCAGTGGCCT CAAAAAACCC ACAAGTGCCT TCAGATGGGG 
     1621 GTGTAAGACT GAATAAATCC AAAACTGACT TACTGGTGAA TGACAACCCA GACCCGGCAC 
     1681 CTCTGTCTCC AGAGCTTCAG GACTTTAAAT GCAATATCTG TGGATATGGT TACTACGGCA 
     1741 ACGACCCCAC AGATCTGATT AAGCACTTCC GAAAGTATCA CTTAGGACTG CATAACCGCA 
     1801 CCAGGCAAGA TGCTGAGCTG GACAGCAAAA TCTTGGCCCT TCATAACATG GTGCAGTTCA 
     1861 GCCATTCCAA AGACTTCCAG AAGGTCAACC GTTCTGTGTT TTCTGGTGTG CTGCAGGACA 
     1921 TCAATTCTTC AAGGCCTGTT TTACTAAATG GGACCTATGA TGTGCAGGTG ACTTCAGGTG 
     1981 GAACATTCAT TGGCATTGGA CGGAAAACAC CAGATTGCCA AGGGAACACC AAGTATTTCC 
     2041 GCTGTAAATT CTGCAATTTC ACTTATATGG GCAACTCATC CACCGAATTA GAACAACATT 
     2101 TTCTTCAGAC TCACCCAAAC AAAATAAAAG CTTCTCTCCC CTCCTCTGAG GTTGCAAAAC 
     2161 CTTCAGAGAA AAACTCTAAC AAGTCCATCC CTGCACTTCA ATCCAGTGAT TCTGGAGACT 
     2221 TGGGAAAATG GCAGGACAAG ATAACAGTCA AAGCAGGAGA TGACACTCCT GTTGGGTACT 
     2281 CAGTGCCCAT AAAGCCCCTC GATTCCTCTA GACAAAATGG TACAGAGGCC ACCAGTTACT 
     2341 ACTGGTGTAA ATTTTGTAGT TTCAGCTGTG AGTCATCTAG CTCACTTAAA CTGCTAGAAC 
     2401 ATTATGGCAA GCAGCACGGA GCAGTGCAGT CAGGCGGCCT TAATCCAGAG TTAAATGATA 
     2461 AGCTTTCCAG GGGCTCTGTC ATTAATCAGA ATGATCTAGC CAAAAGTTCA GAAGGAGAGA 
     2521 CAATGACCAA GACAGACAAG AGCTCGAGTG GGGCTAAAAA GAAGGACTTC TCCAGCAAGG 
     2581 GAGCCGAGGA TAATATGGTA ACGAGCTATA ATTGTCAGTT CTGTGACTTC CGATATTCCA 
     2641 AAAGCCATGG CCCTGATGTA ATTGTAGTGG GGCCACTTCT CCGTCATTAT CAACAGCTCC 
     2701 ATAACATTCA CAAGTGTACC ATTAAACACT GTCCATTCTG TCCCAGAGGA CTTTGCAGCC 
     2761 CAGAAAAGCA CCTTGGAGAA ATTACTTATC CGTTTGCTTG TAGAAAAAGT AATTGTTCCC 
     2821 ACTGTGCACT CTTGCTTCTG CACTTGTCTC CTGGGGCGGC TGGAAGCTCG CGAGTCAAAC 
     2881 ATCAGTGCCA TCAGTGTTCA TTCACCACCC CTGACGTAGA TGTACTCCTC TTTCACTATG 
     2941 AAAGTGTGCA TGAGTCCCAA GCATCGGATG TCAAACAAGA AGCAAATCAC CTGCAAGGAT 
     3001 CGGATGGGCA GCAGTCTGTC AAGGAAAGCA AAGAACACTC ATGTACCAAA TGTGATTTTA 
     3061 TTACCCAAGT GGAAGAAGAG ATTTCCCGAC ACTACAGGAG AGCACACAGC TGCTACAAAT 
     3121 GCCGTCAGTG CAGTTTTACA GCTGCCGATA CTCAGTCACT ACTGGAGCAC TTCAACACTG 
     3181 TTCACTGCCA GGAACAGGAC ATCACTACAG CCAACGGCGA AGAGGACGGT CATGCCATAT 
     3241 CCACCATCAA AGAGGAGCCC AAAATTGACT TCAGGGTCTA CAATCTGCTA ACTCCAGACT 
     3301 CTAAAATGGG AGAGCCAGTT TCTGAGAGTG TGGTGAAGAG AGAGAAGCTG GAAGAGAAGG 
     3361 ACGGGCTCAA AGAGAAAGTT TGGACCGAGA GTTCCAGTGA TGACCTTCGC AATGTGACTT 
     3421 GGAGAGGGGC AGACATCCTG CGGGGGAGTC CGTCATACAC CCAAGCAAGC CTGGGGCTGC 
     3481 TGACGCCTGT GTCTGGCACC CAAGAGCAGA CAAAGACTCT AAGGGATAGT CCCAATGTGG 
     3541 AGGCCGCCCA TCTGGCGCGA CCTATTTATG GCTTGGCTGT GGAAACCAAG GGATTCCTGC 
     3601 AGGGGGCGCC AGCTGGCGGA GAGAAGTCTG GGGCCCTCCC CCAGCAGTAT CCTGCATCGG 
     3661 GAGAAAACAA GTCCAAGGAT GAATCCCAGT CCCTGTTACG GAGGCGTAGA GGCTCCGGTG 
     3721 TTTTTTGTGC CAATTGCCTG ACCACAAAGA CCTCTCTCTG GCGAAAGAAT GCAAATGGCG 
     3781 GATATGTATG CAACGCGTGT GGCCTCTACC AGAAGCTTCA CTCGACTCCC AGGCCTTTAA 
     3841 ACATCATTAA ACAAAACAAC GGTGAGCAGA TTATTAGGAG GAGAACAAGA AAGCGCCTTA 
     3901 ACCCAGAGGC ACTTCAGGCT GAGCAGCTCA ACAAACAGCA GAGGGGCAGC AATGAGGAGC 
     3961 AAGTCAATGG AAGCCCGTTA GAGAGGAGGT CAGAAGATCA TCTAACTGAA AGTCACCAGA 
     4021 GAGAAATTCC ACTCCCCAGC CTAAGTAAAT ACGAAGCCCA GGGTTCATTG ACTAAAAGCC 
     4081 ATTCTGCTCA GCAGCCAGTC CTGGTCAGCC AAACTCTGGA TATTCACAAA AGGATGCAAC 
     4141 CTTTGCACAT TCAGATAAAA AGTCCTCAGG AAAGTACTGG AGATCCAGGA AATAGTTCAT 
     4201 CCGTATCTGA AGGGAAAGGA AGTTCTGAGA GAGGCAGTCC TATAGAAAAG TACATGAGAC 
     4261 CTGCGAAACA CCCAAATTAT TCACCACCAG GCAGCCCTAT TGAAAAGTAC CAGTACCCAC 
     4321 TTTTTGGACT TCCCTTTGTA CATAATGACT TCCAGAGTGA AGCTGATTGG CTGCGGTTCT 
     4381 GGAGTAAATA TAAGCTCTCC GTTCCTGGGA ATCCGCACTA CTTGAGTCAC GTGCCTGGCC 
     4441 TACCAAATCC TTGCCAAAAC TATGTGCCTT ATCCCACCTT CAATCTGCCT CCTCATTTTT 
     4501 CAGCTGTTGG ATCAGACAAT GACATTCCTC TAGATTTGGC GATCAAGCAT TCCAGACCTG 
     4561 GGCCAACTGC AAACGGTGCC TCCAAGGAGA AAACGAAGGC ACCACCAAAT GTAAAAAATG 
     4621 AAGGTCCCTT GAATGTAGTA AAAACAGAGA AAGTTGATAG AAGTACTCAA GATGAACTTT 
     4681 CAACAAAATG TGTGCACTGT GGCATTGTCT TTCTGGATGA AGTGATGTAT GCTTTGCATA 
     4741 TGAGTTGCCA TGGTGACAGT GGACCTTTCC AGTGCAGCAT ATGCCAGCAT CTTTGCACGG 
     4801 ACAAATATGA CTTCACAACA CATATCCAGA GGGGCCTGCA TAGGAACAAT GCACAAGTGG 
     4861 AAAAAAATGG AAAACCTAAA GAGAGCGGAC CGACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4921 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4981 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     5041 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5101 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5161 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5221 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5281 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5341 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5401 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5461 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5521 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5581 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5641 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5701 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5761 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5821 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5881 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5941 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     6001 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6061 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6121 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6181 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6241 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6301 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6361 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6421 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6481 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6541 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6601 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6661 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6721 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6781 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6841 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6901 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6961 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     7021 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7081 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7141 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7201 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7261 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7321 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7381 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7441 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7501 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7561 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7621 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7681 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7741 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7801 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7861 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7921 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7981 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8041 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8101 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8161 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8221 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8281 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8341 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8401 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8461 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8521 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8581 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8641 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8701 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//