LOCUS       RC240093                8730 bp    DNA     circular  SYN 
DEFINITION  MAP3K6 (myc-DDK-tagged) - Human mitogen-activated protein kinase kinase kinase 6 (MAP3K6), transcript variant 2
ACCESSION   NM_001297609
VERSION     NM_001297609.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4887..4978 
                     /label="Myc-DDK"  
     misc_feature    5008..5593 
                     /label="PolyA signal"  
     misc_feature    5065..5084 
                     /label="Restriction Enzyme"  
     misc_feature    5869..6541 
                     /label="ColE1"  
     misc_feature    6971..7765 
                     /label="Kan/Neo"  
     misc_feature    7800..8102 
                     /label="SV40 ori"  
     misc_feature    8188..8665 
                     /label="f1 ori"  
     misc_feature    1029..1037 
                     /label="Restriction Enzyme"  
     misc_feature    4869..4875 
                     /label="Restriction Enzyme"  
     misc_feature    1029..4869 
                     /label="MAP3K6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCGG CGCGCCCATG GCGGGGCCGT GTCCCCGGTC CGGGGCGGAG CGCGCCGGCA 
     1081 GCTGCTGGCA GGACCCGCTG GCCGTGGCGC TGAGCCGGGG CCGGCAGCTC GCGGCGCCCC 
     1141 CGGGCCGGGG CTGCGCGCGG AGCCGGCCGC TCAGCGTGGT CTACGTGCTG ACCCGGGAGC 
     1201 CGCAGCCCGG GCTCGAGCCT CGGGAGGGAA CCGAGGCGGA GCCGCTGCCC CTGCGCTGCC 
     1261 TGCGCGAGGC TTGCGCGCAG GTCCCCCGGC CGCGGCCGCC CCCGCAGCTG CGCAGCCTGC 
     1321 CCTTCGGGAC GCTGGAGCTA GGCGACACCG CGGCTCTGGA TGCCTTCTAC AACGCGGATG 
     1381 TGGTGGTGCT GGAGGTGAGC AGCTCGCTGG TACAGCCCTC CCTGTTCTAC CACCTTGGTG 
     1441 TGCGTGAGAG CTTCAGCATG ACCAACAATG TGCTCCTCTG CTCCCAGGCC GACCTCCCTG 
     1501 ACCTGCAGGC CCTGCGGGAT TGCGTTGGCA GCTACACACT GATCCCCTAT GTGGTGACGG 
     1561 CCACTGGTCG GGTGCTGTGT GGTGATGCAG GCCTTCTGCG GGGCCTGGCT GATGGGCTGG 
     1621 TACAGGCTGG AGTGGGGACC GAGGCCCTGC TCACTCCCCT GGTGGGCCGG CTTGCCCGCC 
     1681 TGCTGGAGGC CACACCCACA GACTCTTGTG GCTATTTCCG GGAGACCATT CGGCGGGACA 
     1741 TCCGGCAGGC GCGGGAGCGG TTCAGTGGGC CACAGCTGCG GCAGGAGCTG GCTCGCCTGC 
     1801 AGCGGAGACT GGACAGCGTG GAGCTGCTGA GCCCCGACAT CATCATGAAC TTGCTGCTCT 
     1861 CCTACCGCGA TGTGCAGGAC TACTCGGCCA TCATTGAGCT GGTGGAGACG CTGCAGGCCT 
     1921 TGCCCACCTG TGATGTGGCC GAGCAGCATA ATGTCTGCTT CCACTACACT TTTGCCCTCA 
     1981 ACCGGAGGAA CAGGCCTGGG GACCGGGCGA AGGCCCTGTC TGTGCTGCTG CCGCTGGTAC 
     2041 AGCTTGAGGG CTCTGTGGCG CCCGATCTGT ACTGCATGTG TGGCCGTATC TACAAGGACA 
     2101 TGTTCTTCAG CTCGGGTTTC CAGGATGCTG GGCACCGGGA GCAGGCCTAT CACTGGTATC 
     2161 GCAAGGCTTT TGACGTAGAG CCCAGCCTTC ACTCAGGCAT CAATGCAGCT GTGCTCCTCA 
     2221 TTGCTGCCGG GCAGCACTTT GAGGATTCCA AAGAGCTCCG GCTAATAGGC ATGAAGCTGG 
     2281 GCTGCCTGCT GGCCCGCAAA GGCTGCGTGG AGAAGATGCA GTATTACTGG GATGTGGGTT 
     2341 TCTACCTGGG AGCCCAGATC CTCGCCAATG ACCCCACCCA GGTGGTGCTG GCTGCAGAGC 
     2401 AGCTGTATAA GCTCAATGCC CCCATATGGT ACCTGGTGTC CGTGATGGAG ACCTTCCTGC 
     2461 TCTACCAGCA CTTCAGGCCC ACGCCAGAGC CCCCTGGAGG GCCACCACGC CGTGCCCACT 
     2521 TCTGGCTCCA CTTCTTGCTA CAGTCCTGCC AACCATTCAA GACAGCCTGT GCCCAGGGCG 
     2581 ACCAGTGCTT GGTGCTGGTC CTGGAGATGA ACAAGGTGCT GCTGCCTGCA AAGCTCGAGG 
     2641 TTCGGGGTAC TGACCCAGTA AGCACAGTGA CCCTGAGCCT GCTGGAGCCT GAGACCCAGG 
     2701 ACATTCCCTC CAGCTGGACC TTCCCAGTCG CCTCCATATG CGGAGTCAGC GCCTCAAAGC 
     2761 GCGACGAGCG CTGCTGCTTC CTCTATGCAC TCCCCCCGGC TCAGGACGTC CAGCTGTGCT 
     2821 TCCCCAGCGT AGGGCACTGC CAGTGGTTCT GCGGCCTGAT CCAGGCCTGG GTGACGAACC 
     2881 CGGATTCCAC GGCGCCCGCG GAGGAGGCGG AGGGCGCGGG GGAGATGTTG GAGTTTGATT 
     2941 ATGAGTACAC GGAGACGGGC GAGCGGCTGG TGCTGGGCAA GGGCACGTAT GGGGTGGTGT 
     3001 ACGCGGGCCG CGATCGCCAC ACGAGGGTGC GCATCGCCAT CAAGGAGATC CCGGAGCGGG 
     3061 ACAGCAGGTT CTCTCAGCCC CTGCATGAAG AGATCGCTCT TCACAGACGC CTGCGCCACA 
     3121 AGAACATAGT GCGCTATCTG GGCTCAGCTA GCCAGGGCGG CTACCTTAAG ATCTTCATGG 
     3181 AGGAAGTGCC TGGAGGCAGC CTGTCCTCCT TGCTGCGGTC GGTGTGGGGA CCCCTGAAGG 
     3241 ACAACGAGAG CACCATCAGT TTCTACACCC GCCAGATCCT GCAGGGACTT GGCTACTTGC 
     3301 ACGACAACCA CATCGTGCAC AGGGACATAA AAGGGGACAA TGTGCTGATC AACACCTTCA 
     3361 GTGGGCTGCT CAAGATTTCT GACTTCGGCA CCTCCAAGCG GCTGGCAGGC ATCACACCTT 
     3421 GCACTGAGAC CTTCACAGGA ACTCTGCAGT ATATGGCCCC AGAAATCATT GACCAGGGCC 
     3481 CACGCGGGTA TGGGAAAGCA GCTGACATCT GGTCACTGGG CTGCACTGTC ATTGAGATGG 
     3541 CCACAGGTCG CCCCCCCTTC CACGAGCTCG GGAGCCCACA GGCTGCCATG TTTCAGGTGG 
     3601 GTATGTACAA GGTCCATCCG CCAATGCCCA GCTCTCTGTC GGCCGAGGCC CAAGCCTTTC 
     3661 TCCTCCGAAC TTTTGAGCCA GACCCCCGCC TCCGAGCCAG CGCCCAGACA CTGCTGGGGG 
     3721 ACCCCTTCCT GCAGCCTGGG AAAAGGAGCC GCAGCCCCAG CTCCCCACGA CATGCTCCAC 
     3781 GGCCCTCAGA TGCCCCTTCT GCCAGTCCCA CTCCTTCAGC CAACTCAACC ACCCAGTCTC 
     3841 AGACATTCCC GTGCCCTCAG GCACCCTCTC AGCACCCACC CAGCCCCCCG AAGCGCTGCC 
     3901 TCAGTTATGG GGGCACCAGC CAGCTCCGGG TGCCCGAGGA GCCTGCGGCC GAGGAGCCTG 
     3961 CGTCTCCGGA GGAGAGTTCG GGGCTGAGCC TGCTGCACCA GGAGAGCAAG CGTCGGGCCA 
     4021 TGCTGGCCGC AGTATTGGAG CAGGAGCTGC CAGCGCTGGC GGAGAATCTG CACCAGGAGC 
     4081 AGAAGCAAGA GCAGGGGGCC CGTCTGGGCA GAAACCATGT GGAAGAGCTG CTGCGCTGCC 
     4141 TCGGGGCACA CATCCACACT CCCAACCGCC GGCAGCTCGC CCAGGAGCTG CGGGCGCTGC 
     4201 AAGGACGGCT GAGGGCCCAG GGCCTTGGGC CTGCGCTTCT GCACAGACCG CTGTTTGCCT 
     4261 TCCCGGATGC GGTGAAGCAG ATCCTCCGCA AGCGCCAGAT CCGTCCACAC TGGATGTTCG 
     4321 TTCTGGACTC ACTGCTCAGC CGTGCTGTGC GGGCAGCCCT GGGTGTGCTA GGACCGGAGG 
     4381 TGGAGAAGGA GGCGGTCTCA CCGAGGTCAG AGGAGCTGAG TAATGAAGGG GACTCCCAGC 
     4441 AGAGCCCAGG CCAGCAGAGC CCGCTTCCGG TGGAGCCCGA GCAGGGCCCC GCTCCTCTGA 
     4501 TGGTGCAGCT GAGCCTCTTG AGGGCAGAGA CTGATCGGCT GCGCGAAATC CTGGCGGGGA 
     4561 AGGAACGGGA GTACCAGGCC CTGGTGCAGC GGGCTCTACA GCGGCTGAAT GAGGAAGCCC 
     4621 GGACCTATGT CCTGGCCCCA GAGCCTCCAA CTGCTCTTTC AACGGACCAG GGCCTGGTGC 
     4681 AGTGGCTACA GGAACTGAAT GTGGATTCAG GCACCATCCA AATGCTGTTG AACCATAGCT 
     4741 TCACCCTCCA CACTCTGCTC ACCTATGCCA CTCGAGATGA CCTCATCTAC ACCCGCATCA 
     4801 GGGGAGGGAT GGTATGCCGC ATCTGGAGGG CCATCTTGGC ACAGCGAGCA GGATCCACAC 
     4861 CAGTCACCTC TGGACCCACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4921 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4981 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5041 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5101 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5161 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5221 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5281 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5341 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5401 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5461 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5521 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5581 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5641 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5701 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5761 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5821 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5881 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5941 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6001 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6061 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6121 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6181 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6241 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6301 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6361 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6421 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6481 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6541 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6601 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6661 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6721 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6781 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6841 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6901 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6961 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7021 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7081 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7141 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7201 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7261 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7321 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7381 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7441 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7501 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7561 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7621 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7681 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7741 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7801 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7861 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7921 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7981 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8041 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8101 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8161 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8221 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8281 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8341 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8401 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8461 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8521 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8581 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8641 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8701 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//