LOCUS       RC240083                8679 bp    DNA     circular  SYN 
DEFINITION  SORBS1 (myc-DDK-tagged) - Human sorbin and SH3 domain containing 1 (SORBS1), transcript variant 8
ACCESSION   NM_001290294
VERSION     NM_001290294.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4836..4927 
                     /label="Myc-DDK"  
     misc_feature    4957..5542 
                     /label="PolyA signal"  
     misc_feature    5014..5033 
                     /label="Restriction Enzyme"  
     misc_feature    5818..6490 
                     /label="ColE1"  
     misc_feature    6920..7714 
                     /label="Kan/Neo"  
     misc_feature    7749..8051 
                     /label="SV40 ori"  
     misc_feature    8137..8614 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4818..4824 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4818 
                     /label="SORBS1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTTCTGAA TGTGATGGTG GTTCCAAAGC TGTGATGAAT GGCTTGGCAC 
     1081 CTGGCAGCAA TGGGCAAGAC AAAGCAACTG CCGACCCTTT ACGCGCACGC TCTATTTCTG 
     1141 CTGTTAAAAT CATTCCTGTG AAGACAGTGA AAAACGCCTC AGGCCTAGTT CTCCCTACAG 
     1201 ACATGGATCC TACAAAAATC TGCACTGGGA AGGGAGCGGT GACTCTCCGG GCCTCGTCTT 
     1261 CCTACAGGGA AACCCCAAGC AGTAGCCCTG CGAGCCCTCA GGAAACCCGG CAACACGAAA 
     1321 GCAAACCAGG TCTGGAGCCA GAGCCTTCTT CAGCAGATGA GTGGAGGCTT TCTTCCAGTG 
     1381 CTGATGCCAA TGGAAATGCC CAGCCCTCTT CACTCGCTGC CAAGGGCTAC AGAAGTGTGC 
     1441 ATCCCAACCT TCCTTCTGAC AAGTCCCAGG ATGCCACTTC CTCCAGTGCA GCCCAGCCGG 
     1501 AGGTAATAGT TGTCCCTCTC TACCTGGTTA ATACTGACAG AGGGCAAGAA GGCACTGCCA 
     1561 GACCTCCAAC ACCTCTGGGG CCTCTTGGCT GCGTCCCCAC AATCCCAGCG ACTGCCTCTG 
     1621 CCGCCTCACC TCTGACCTTC CCGACTCTAG ATGATTTCAT TCCCCCTCAT CTGCAGAGGT 
     1681 GGCCCCACCA CAGCCAGCCA GCCCGCGCCT CTGGCTCCTT TGCCCCCATT AGCCAGACGC 
     1741 CACCATCCTT CTCACCACCA CCTCCGCTGG TCCCTCCTGC CCCGGAGGAC CTCCGCAGAG 
     1801 TCTCGGAGCC TGACCTCACG GGAGCTGTTT CGAGTACCGA TTCCAGTCCT CTACTAAATG 
     1861 AAGTTTCTTC TTCCCTTATT GGAACTGATT CCCAAGCCTT TCCATCAGTT AGCAAGCCTT 
     1921 CATCCGCCTA TCCCTCCACA ACGATTGTCA ATCCTACTAT TGTGCTCTTG CAACACAATC 
     1981 GAGAACAGCA AAAACGACTC AGTAGCCTTT CAGATCCTGT CTCAGAAAGA AGAGTGGGAG 
     2041 AGCAGGACTC AGCACCAACC CAGGAAAAAC CCACCTCACC TGGCAAGGCT ATTGAAAAAA 
     2101 GAGCAAAGGA TGACAGTAGG CGGGTGGTGA AGAGCACTCA GGACTTAAGC GATGTTTCCA 
     2161 TGGATGAAGT GGGCATCCCA CTCCGGAACA CTGAGAGATC AAAAGACTGG TACAAGACTA 
     2221 TGTTTAAACA GATCCACAAA CTGAACAGAG ATGATGATTC AGATCTGTAC TCTCCCAGAT 
     2281 ACTCATTTTC TGAAGACACA AAATCTCCCC TTTCTGTGCC TCGCTCAAAA AGTGAGATGA 
     2341 GCTACATTGA TGGTGAGAAG GTAGTCAAGA GGTCGGCCAC ACTACCCCTC CCAGCCCGCT 
     2401 CTTCCTCACT GAAGTCAAGC TCAGAAAGAA ATGACTGGGA ACCCCCAGAT AAGAAAGTAG 
     2461 ACACAAGAAA ATATCGTGCA GAGCCCAAGA GCATTTACGA ATATCAGCCT GGCAAGTCTT 
     2521 CCGTTCTGAC CAACGAAAAG ATGAGTCGGG ATATAAGCCC AGAAGAGATA GATTTAAAGA 
     2581 ATGAACCTTG GTATAAATTC TTTTCGGAAT TGGAGTTTGG GAAACCGCCT CCCAAAAAGA 
     2641 TATGGGATTA TACTCCTGGA GACTGCTCTA TCCTTCCTAG AGAGGATAGA AAGACTAATC 
     2701 TAGACAAAGA TCTCAGCCTC TGCCAGACAG AGTTAGAGGC AGATTTAGAA AAAATGGAGA 
     2761 CGCTTAATAA AGCACCCAGT GCAAACGTGC CACAGAGCTC AGCCATCAGC CCTACTCCGG 
     2821 AAATTTCTTC AGAGACTCCT GGATATATAT ATTCTTCCAA CTTCCATGCA GTGAAGAGGG 
     2881 AATCAGACGG GGCTCCTGGG GATCTCACTA GCTTGGAGAA TGAGAGACAA ATTTATAAAA 
     2941 GTGTCTTGGA AGGTGGTGAC ATCCCTCTTC AGGGCCTGAG TGGGCTCAAG CGACCATCCA 
     3001 GCTCTGCTTC CACTAAAGAT TCAGAATCGC CAAGACATTT TATACCAGCT GATTACTTGG 
     3061 AATCCACGGA AGAATTTATT CGAAGACGTC ATGATGATAA AGAGAAACTT TTAGCGGACC 
     3121 AGAGACGACT TAAACGCGAG CAAGAAGAGG CTGATATTGC AGCTCGACGC CACACAGGCG 
     3181 TCATTCCGAC GCACCATCAG TTTATCACTA ATGAGCGCTT TGGGGACCTC CTCAATATAG 
     3241 ACGATACTGC AAAAAGGAAA TCTGGGTCAG AGATGAGACC TGCCAGAGCC AAATTTGACT 
     3301 TTAAAGCTCA GACACTAAAG GAGCTTCCTC TGCAGAAGGG AGATATTGTT TACATTTATA 
     3361 AGCAAATTGA TCAGAACTGG TATGAAGGAG AACACCACGG CCGGGTGGGA ATCTTCCCAC 
     3421 GCACCTACAT CGAGCTTCTT CCTCCTGCTG AGAAGGCACA GCCCAAAAAG TTGACACCAG 
     3481 TGCAGGTTTT GGAATATGGA GAAGCTATTG CTAAGTTTAA CTTTAATGGT GATACACAAG 
     3541 TAGAAATGTC CTTCAGAAAG GGTGAGAGGA TCACACTGCT CCGGCAGGTA GATGAGAACT 
     3601 GGTACGAAGG GAGGATCCCG GGGACATCCC GACAAGGCAT CTTCCCCATC ACCTACGTGG 
     3661 ATGTGATCAA GCGACCACTG GTGAAAAACC CTGTGGATTA CATGGACCTG CCTTTCTCCT 
     3721 CCTCCCCAAG TCGCAGTGCC ACTGCAAGCC CACAGTTTTC CAGTCACAGC AAGCTCATCA 
     3781 CGCCAGCCCC CTCATCTCTG CCCCACTCCC GCCGAGCCCT GTCCCCCGAG ATGCACGCTG 
     3841 TCACCTCTGA GTGGATCTCA CTGACTGTGG GGGTCCCAGG CAGGCGTTCT CTGGCCCTGA 
     3901 CCCCACCCTT GCCTCCTCTG CCAGAGGCTT CTATCTATAA CACTGACCAC CTCGCCTTGT 
     3961 CACCAAGGGC CAGTCCCTCC CTGTCTCTCA GCCTCCCCCA TTTGAGTTGG TCAGATCGTC 
     4021 CCACCCCACG ATCAGTAGCT TCTCCACTGG CCCTACCTTC CCCACACAAA ACCTACTCCC 
     4081 TAGCACCTAC TTCCCAGGCC TCCCTTCACA TGAATGGAGA CGGTGGTGTC CACACGCCAT 
     4141 CTTCAGGCAT CCACCAAGAT AGCTTCTTGC AGCTGCCGCT GGGGAGCTCT GATAGTGTCA 
     4201 TCTCCCAGCT TAGTGATGCC TTTAGCAGCC AGAGCAAGAG GCAGCCATGG CGCGAAGAGA 
     4261 GTGGACAATA TGAGAGGAAA GCAGAGAGGG GGGCAGGCGA AAGAGGCCCT GGTGGACCCA 
     4321 AGATCTCTAA GAAGAGCTGC TTGAAGCCTT CAGACGTGGT CAGGTGCCTG AGTACTGAAC 
     4381 AGAGACTCTC AGATCTCAAC ACCCCTGAGG AGAGCCGGCC CGGCAAGCCC CTGGGTAGCG 
     4441 CTTTTCCAGG AAGTGAGGCT GAGCAGACAG AGCGGCATAG AGGTGGCGAG CAGGCGGGGA 
     4501 GGAAAGCTGC TCGGAGAGGT GGGAGCCAGC TTTCTCATCA TTCATTGAGA GCAGGACCAG 
     4561 ATCTCACAGA ATCTGAAAAG AGCTATGTGC AACCTCAAGC CCAGCAGCGA AGAGTCACCC 
     4621 CCGACAGGAG TCAAACCTCA CAAGATTTAT TTAGCTATCA AGCATTATAT AGCTATATAC 
     4681 CACAGAATGA TGATGAGTTG GAACTCCGCG ATGGAGATAT CGTTGATGTC ATGGAAAAAT 
     4741 GTGACGATGG ATGGTTTGTT GGTACTTCAA GAAGGACAAA GCAGTTTGGT ACTTTTCCAG 
     4801 GCAACTATGT AAAACCTTTG TATCTAACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4861 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4921 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4981 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     5041 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     5101 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     5161 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     5221 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5281 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5341 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5401 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5461 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5521 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5581 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5641 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5701 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5761 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5821 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5881 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5941 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     6001 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     6061 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     6121 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     6181 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6241 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6301 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6361 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6421 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6481 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6541 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6601 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6661 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6721 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6781 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6841 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6901 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6961 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     7021 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     7081 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     7141 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     7201 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7261 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7321 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7381 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7441 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7501 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7561 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7621 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7681 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7741 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7801 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7861 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7921 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7981 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8041 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     8101 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     8161 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     8221 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     8281 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8341 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8401 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8461 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8521 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8581 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8641 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//