LOCUS       RC240080                8667 bp    DNA     circular  SYN 
DEFINITION  MYO6 (myc-DDK-tagged) - Human myosin VI (MYO6), transcript variant 2
ACCESSION   NM_001300899
VERSION     NM_001300899.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4824..4915 
                     /label="Myc-DDK"  
     misc_feature    4945..5530 
                     /label="PolyA signal"  
     misc_feature    5002..5021 
                     /label="Restriction Enzyme"  
     misc_feature    5806..6478 
                     /label="ColE1"  
     misc_feature    6908..7702 
                     /label="Kan/Neo"  
     misc_feature    7737..8039 
                     /label="SV40 ori"  
     misc_feature    8125..8602 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4806..4812 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4806 
                     /label="MYO6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGATGGA AAGCCCGTTT GGGCGCCACA CCCTACAGAT GGATTTCAGA 
     1081 TGGGCAATAT TGTGGATATT GGCCCCGACA GCTTAACAAT TGAACCCTTG AATCAGAAAG 
     1141 GCAAGACATT TTTGGCTCTC ATAAACCAAG TGTTTCCTGC AGAAGAGGAC AGTAAAAAAG 
     1201 ATGTGGAAGA TAACTGTTCA CTAATGTATT TAAATGAAGC CACACTGCTC CATAATATCA 
     1261 AAGTTCGATA TAGTAAAGAC AGAATTTATA CATATGTCGC CAACATTCTG ATTGCAGTGA 
     1321 ATCCATACTT TGACATACCT AAAATATATT CTTCAGAAGC AATAAAGTCA TATCAAGGAA 
     1381 AATCTCTTGG GACAAGACCA CCTCATGTCT TTGCAATTGC TGATAAAGCT TTTCGAGACA 
     1441 TGAAGGTGCT CAAGATGAGT CAGTCTATCA TTGTATCTGG AGAATCAGGA GCCGGCAAAA 
     1501 CAGAAAATAC AAAATTTGTT CTAAGATACC TGACTGAATC CTATGGAACA GGTCAAGATA 
     1561 TTGATGACAG AATTGTTGAA GCTAACCCAC TCCTAGAAGC CTTTGGAAAT GCGAAGACTG 
     1621 TTCGCAACAA TAATAGCAGT CGATTTGGGA AATTTGTAGA AATACATTTT AATGAAAAGA 
     1681 GCTCAGTTGT TGGAGGATTT GTTTCACATT ATCTCCTAGA GAAATCTAGG ATCTGTGTTC 
     1741 AAGGCAAAGA GGAAAGAAAT TATCATATCT TTTATAGGTT GTGTGCTGGT GCTTCTGAAG 
     1801 ATATTAGAGA AAAACTTCAT TTGAGTTCAC CAGATAATTT TCGGTATTTA AACCGAGGCT 
     1861 GCACTAGATA CTTTGCTAAC AAAGAAACTG ACAAACAGAT TTTACAGAAC CGCAAAAGTC 
     1921 CTGAGTACCT TAAGGCAGGT TCTATGAAAG ATCCTCTGCT AGATGACCAT GGTGATTTTA 
     1981 TTAGAATGTG CACGGCTATG AAAAAAATTG GTTTGGATGA TGAAGAAAAG CTTGATCTCT 
     2041 TCCGGGTAGT AGCTGGCGTC CTGCACCTTG GAAATATTGA TTTTGAGGAA GCTGGCAGCA 
     2101 CTTCAGGTGG TTGTAATCTG AAGAATAAAT CTGCTCAGTC TTTGGAATAT TGTGCTGAAT 
     2161 TACTGGGTTT GGACCAAGAT GATCTTCGAG TAAGTTTGAC CACAAGAGTC ATGCTAACAA 
     2221 CAGCAGGGGG CACCAAAGGA ACAGTTATAA AGGTACCTCT GAAAGTGGAG CAAGCAAACA 
     2281 ATGCTCGTGA TGCCCTGGCA AAGACAGTGT ATAGCCATCT TTTTGATCAT GTGGTAAACA 
     2341 GAGTAAATCA GTGTTTTCCT TTTGAAACAT CATCCTATTT TATTGGAGTC CTAGATATTG 
     2401 CTGGTTTTGA GTACTTTGAG CATAACAGTT TTGAACAATT TTGCATCAAC TATTGCAATG 
     2461 AAAAACTTCA ACAATTTTTT AATGAAAGGA TTCTGAAGGA GGAACAAGAA CTCTATCAAA 
     2521 AAGAAGGTTT AGGTGTTAAT GAAGTGCATT ATGTGGATAA TCAGGACTGT ATAGATTTAA 
     2581 TTGAAGCCAA ATTAGTGGGA ATACTGGATA TTTTGGATGA AGAAAATCGC CTTCCCCAGC 
     2641 CAAGTGATCA ACACTTTACA TCTGCAGTTC ACCAAAAGCA CAAGGATCAT TTTCGACTCA 
     2701 CTATTCCCAG AAAATCTAAG CTGGCAGTTC ATAGGAATAT CAGAGACGAC GAAGGCTTCA 
     2761 TTATCAGGCA TTTTGCGGGG GCAGTGTGCT ATGAAACAAC CCAGTTTGTG GAGAAAAATA 
     2821 ATGATGCTTT ACATATGTCT CTTGAATCCT TAATATGTGA ATCCAGAGAT AAGTTTATAC 
     2881 GGGAATTATT TGAATCATCC ACAAATAACA ACAAAGATAC TAAACAAAAA GCAGGAAAAC 
     2941 TTAGCTTCAT CAGCGTGGGA AACAAGTTTA AGACACAGTT AAATTTGCTT CTGGATAAAC 
     3001 TTCGAAGTAC TGGAGCAAGC TTTATTCGTT GCATCAAACC TAACTTAAAG ATGACAAGCC 
     3061 ACCACTTTGA AGGTGCTCAA ATTCTGTCTC AGCTTCAGTG TTCAGGGATG GTGTCTGTTT 
     3121 TGGACTTGAT GCAGGGTGGT TACCCATCAC GAGCTTCATT TCATGAACTC TACAACATGT 
     3181 ACAAAAAGTA TATGCCAGAT AAACTTGCAA GATTGGATCC AAGACTATTT TGTAAGGCTT 
     3241 TGTTTAAAGC TTTGGGCTTA AATGAAAATG ACTACAAGTT TGGGTTAACC AAAGTATTTT 
     3301 TTAGACCTGG CAAGTTTGCA GAATTTGATC AGATCATGAA GTCTGACCCT GACCACTTAG 
     3361 CAGAGTTGGT TAAAAGAGTC AATCACTGGC TCACATGCAG TCGCTGGAAG AAAGTTCAGT 
     3421 GGTGCTCACT CTCAGTCATC AAATTGAAAA ACAAAATAAA ATATCGAGCT GAAGCCTGCA 
     3481 TTAAAATGCA AAAAACTATT CGAATGTGGC TTTGCAAGAG GAGACACAAA CCTCGCATTG 
     3541 ATGGTCTGGT TAAGGTGGGC ACACTGAAAA AACGACTTGA TAAATTTAAT GAGGTAGTCA 
     3601 GTGTGTTGAA AGATGGAAAA CCCGAGATGA ATAAACAGAT CAAGAATCTG GAAATTTCTA 
     3661 TTGATACTTT GATGGCCAAA ATTAAGTCCA CTATGATGAC GCAGGAACAA ATCCAGAAAG 
     3721 AATATGATGC ACTGGTTAAA AGCTCAGAGG AACTCCTCAG TGCATTACAG AAAAAAAAAC 
     3781 AGCAGGAAGA GGAAGCAGAA AGGCTGAGGC GTATTCAAGA AGAAATGGAA AAGGAAAGAA 
     3841 AAAGACGTGA AGAAGACGAA AAACGTCGAA GAAAGGAAGA GGAGGAAAGG CGGATGAAAC 
     3901 TTGAGATGGA AGCAAAGAGA AAACAAGAAG AAGAAGAGAG AAAGAAAAGG GAAGATGATG 
     3961 AAAAACGCAT TCAAGCTGAA GTGGAGGCAC AGCTGGCCCG ACAGAAGGAG GAGGAATCCC 
     4021 AACAGCAAGC AGTTCTGGAG CAGGAGCGCA GGGACCGGGA GCTGGCCCTG AGGATTGCCC 
     4081 AGAGTGAAGC CGAGCTCATC AGTGATGAGG CCCAGGCCGA CCTGGCGCTG CGGAGAGGTC 
     4141 CTGCTGTACT AGCCACCAAA GCAGCTGCTG GTACTAAGAA ATATGATCTT AGTAAATGGA 
     4201 AATATGCAGA ACTACGTGAT ACCATCAATA CTTCTTGTGA TATTGAGCTC CTGGCAGCTT 
     4261 GCAGAGAAGA ATTTCATAGG AGACTAAAAG TGTATCATGC TTGGAAATCT AAGAACAAGA 
     4321 AGAGAAATAC TGAAACAGAG CAACGTGCTC CAAAGTCTGT TACTGATTAT GATTTTGCAC 
     4381 CATTTTTGAA CAATTCACCT CAGCAAAACC CAGCAGCTCA GATTCCTGCC AGGCAGCGGG 
     4441 AGATTGAAAT GAACCGACAG CAACGCTTCT TCCGCATCCC ATTCATCCGC CCTGCCGACC 
     4501 AGTACAAAGA CCCTCAGAGT AAGAAAAAAG GCTGGTGGTA TGCCCATTTT GATGGACCAT 
     4561 GGATTGCCCG GCAAATGGAA CTCCATCCTG ACAAGCCACC CATCCTACTT GTGGCTGGTA 
     4621 AGGACGACAT GGAGATGTGT GAGCTGAATC TTGAGGAGAC TGGCCTGACT CGGAAGCGTG 
     4681 GTGCTGAGAT CTTGCCAAGA CAGTTTGAAG AAATCTGGGA ACGCTGTGGA GGCATCCAGT 
     4741 ACCTTCAGAA TGCGATTGAG AGCAGACAGG CTCGGCCCAC CTATGCAACA GCCATGCTGC 
     4801 AGAGTCTGTT AAAGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4861 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4921 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4981 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     5041 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     5101 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5161 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5221 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5281 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5341 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5401 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5461 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5521 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5581 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5641 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5701 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5761 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5821 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5881 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5941 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     6001 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     6061 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6121 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6181 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6241 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6301 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6361 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6421 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6481 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6541 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6601 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6661 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6721 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6781 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6841 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6901 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6961 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     7021 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     7081 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7141 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7201 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7261 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7321 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7381 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7441 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7501 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7561 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7621 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7681 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7741 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7801 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7861 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7921 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7981 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8041 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     8101 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8161 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8221 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8281 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8341 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8401 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8461 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8521 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8581 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8641 ATTTAACAAA AATTTAACGC GAATTTT
//