LOCUS       RC240074                8616 bp    DNA     circular  SYN 
DEFINITION  CNGB1 (myc-DDK-tagged) - Human cyclic nucleotide gated channel beta 1 (CNGB1), transcript variant 3
ACCESSION   NM_001286130
VERSION     NM_001286130.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4773..4864 
                     /label="Myc-DDK"  
     misc_feature    4894..5479 
                     /label="PolyA signal"  
     misc_feature    4951..4970 
                     /label="Restriction Enzyme"  
     misc_feature    5755..6427 
                     /label="ColE1"  
     misc_feature    6857..7651 
                     /label="Kan/Neo"  
     misc_feature    7686..7988 
                     /label="SV40 ori"  
     misc_feature    8074..8551 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4755..4761 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4755 
                     /label="CNGB1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTGGGCTGG GTCCAGAGGG TGCTGCCTCA GCCCCCAGGG ACCCCTCGGA 
     1081 AGACCAAGAT GCAGGAGGAA GAGGAAGTGG AACCAGAGCC AGAGATGGAG GCGGAGGTGG 
     1141 AACCAGAACC GAATCCTGAG GAGGCCGAGA CAGAGTCCGA GTCCATGCCC CCCGAAGAGT 
     1201 CATTCAAGGA GGAGGAAGTG GCTGTGGCAG ACCCAAGCCC TCAGGAGACC AAGGAGGCTG 
     1261 CCCTTACTTC CACCATATCC CTCCGGGCCC AGGGCGCTGA GATTTCTGAA ATGAATAGTC 
     1321 CCAGCCGCAG GGTACTGACC TGGCTCATGA AGGGCGTAGA GAAGGTGATC CCGCAGCCTG 
     1381 TTCACAGCAT CACGGAGGAC CCGGCTCAGA TCCTGGGGCA TGGCAGCACT GGGGACACAG 
     1441 GGTGCACAGA TGAACCCAAT GAGGCCCTTG AGGCCCAAGA CACTAGGCCT GGGCTGCGGC 
     1501 TGCTTCTGTG GCTGGAGCAG AATCTGGAAA GAGTGCTTCC TCAGCCCCCC AAATCCTCTG 
     1561 AGGTCTGGAG AGATGAGCCT GCAGTTGCTA CAGCGCCTCC AGGACGCCCC CAGGAAATGG 
     1621 GGCCCAAGCT GCAGGCCCGG GAGACCCCCT CCCTGCCCAC ACCCATCCCC CTGCAGCCCA 
     1681 AGGAGGAACC CAAGGAGGCA CCAGCTCCAG AGCCCCAGCC CGGCTCCCAG GCCCAGACCT 
     1741 CCTCCCTGCC ACCAACCAGG GACCCTGCCA GGCTGGTGGC ATGGGTCCTG CACAGGCTGG 
     1801 AGATGGCCTT GCCGCAGCCA GTGCTACATG GGAAAATAGG GGAACAGGAG CCTGACTCCC 
     1861 CTGGGATATG TGATGTGCAG ACCATCAGCA TCCTTCCTGG AGGACAAGTG GAGCCTGACC 
     1921 TTGTCCTAGA GGAGGTTGAA CCGCCCTGGG AGGATGCCCA CCAGGATGTC AGTACCAGCC 
     1981 CACAGGGTAC AGAGGTGGTT CCAGCTTATG AAGAAGAGAA CAAAGCTGTG GAGAAGATGC 
     2041 CCAGAGAGCT GTCCCGGATT GAAGAGGAGA AAGAAGATGA GGAGGAGGAA GAGGAAGAGG 
     2101 AGGAGGAGGA GGAAGAGGAG GAGGTGACTG AGGTGCTGCT GGATAGCTGT GTGGTGTCGC 
     2161 AGGTGGGCGT GGGCCAGAGT GAAGAAGACG GGACCCGGCC CCAGAGCACT TCAGATCAGA 
     2221 AGCTGTGGGA GGAAGTTGGG GAGGAGGCCA AGAAGGAGGC TGAAGAGAAG GCCAAGGAGG 
     2281 AGGCCGAGGA GGTGGCTGAA GAGGAGGCTG AAAAGGAGCC CCAGGACTGG GCGGAGACCA 
     2341 AGGAGGAGCC TGAGGCTGAG GCCGAGGCTG CCAGTTCAGG AGTGCCTGCC ACGAAACAGC 
     2401 ACCCAGAAGT GCAGGTGGAA GATACTGATG CTGATAGCTG CCCCCTCATG GCAGAAGAGA 
     2461 ATCCACCCTC AACCGTGTTG CCGCCACCGT CTCCTGCCAA ATCAGACACC CTTATAGTCC 
     2521 CAAGCTCAGC CTCGGGGACA CACAGGAAGA AGCTGCCCTC TGAGGATGAT GAGGCTGAAG 
     2581 AGCTCAAGGC GTTGTCACCA GCAGAGTCCC CAGTGGTTGC CTGGTCTGAC CCCACCACCC 
     2641 CGAAGGACAC TGATGGCCAG GACCGTGCGG CCTCCACGGC CAGCACAAAT AGCGCCATCA 
     2701 TCAACGACCG GCTCCAGGAG CTGGTGAAGC TCTTCAAGGA GCGGACAGAG AAAGTGAAGG 
     2761 AGAAACTCAT TGACCCTGAC GTCACCTCTG ATGAGGAGAG CCCCAAGCCC TCCCCAGCCA 
     2821 AGAAAGCCCC AGAGCCAGCT CCAGACACAA AGCCCGCTGA AGCCGAGCCA GTGGAAGAGG 
     2881 AGCACTATTG CGACATGCTC TGCTGCAAGT TCAAACACCG CCCCTGGAAG AAGTACCAGT 
     2941 TTCCCCAGAG CATTGACCCG CTGACCAACC TGATGTATGT CCTATGGCTG TTCTTCGTGG 
     3001 TGATGGCCTG GAATTGGAAC TGTTGGCTGA TTCCCGTGCG CTGGGCCTTC CCCTACCAGA 
     3061 CCCCGGACAA CATCCACCAC TGGCTGCTGA TGGATTACCT ATGCGACCTC ATCTACTTCC 
     3121 TGGACATCAC CGTGTTCCAG ACACGCCTGC AGTTTGTCAG AGGCGGGGAC ATCATTACGG 
     3181 ACAAAAAGGA CATGCGAAAT AACTACCTGA AGTCTCGCCG CTTCAAGATG GACCTGCTCA 
     3241 GCCTCCTGCC CTTGGATTTT CTCTATTTGA AAGTCGGTGT GAACCCCCTC CTCCGCCTGC 
     3301 CCCGCTGTTT AAAGTACATG GCCTTCTTCG AGTTTAACAG CCGCCTGGAA TCCATCCTCA 
     3361 GCAAAGCCTA CGTGTACAGG GTCATCAGGA CCACAGCCTA CCTTCTCTAC AGCCTGCATT 
     3421 TGAATTCCTG TCTTTATTAC TGGGCATCGG CCTATCAGGG CCTCGGCTCC ACTCACTGGG 
     3481 TTTACGATGG CGTGGGAAAC AGTTATATTC GCTGTTACTA CTTTGCTGTG AAGACCCTCA 
     3541 TCACCATCGG GGGGCTGCCT GACCCCAAGA CACTCTTTGA AATTGTCTTC CAGCTGCTGA 
     3601 ATTATTTCAC GGGCGTCTTT GCTTTCTCTG TGATGATCGG ACAGATGAGA GATGTGGTAG 
     3661 GGGCCGCCAC CGCCGGACAG ACCTACTACC GCAGCTGCAT GGACAGCACG GTGAAGTACA 
     3721 TGAATTTCTA CAAGATCCCC AAGTCCGTGC AGAACCGCGT CAAGACCTGG TACGAGTACA 
     3781 CCTGGCACTC GCAAGGCATG CTGGATGAGT CAGAGCTGAT GGTGCAGCTT CCAGACAAGA 
     3841 TGCGGCTGGA CCTCGCCATC GACGTGAACT ACAACATCGT TAGCAAAGTC GCACTCTTTC 
     3901 AGGGCTGTGA CCGGCAGATG ATCTTTGACA TGCTGAAGAG GCTTCGCTCT GTTGTCTACC 
     3961 TGCCCAACGA CTATGTGTGC AAGAAGGGGG AGATCGGCCG TGAGATGTAC ATCATCCAGG 
     4021 CAGGGCAAGT GCAGGTCTTG GGCGGCCCTG ATGGGAAATC TGTGCTGGTG ACGCTGAAAG 
     4081 CTGGATCTGT GTTTGGAGAA ATAAGCTTGC TGGCTGTTGG GGGCGGGAAC CGGCGCACGG 
     4141 CCAACGTGGT GGCGCACGGG TTTACCAACC TCTTCATCCT GGATAAGAAG GACCTGAATG 
     4201 AGATTTTGGT GCATTATCCT GAGTCTCAGA AGTTACTCCG GAAGAAAGCC AGGCGCATGC 
     4261 TGAGAAGCAA CAATAAGCCC AAGGAGGAGA AGAGCGTGCT GATCCTTCCA CCCCGGGCGG 
     4321 GCACCCCAAA GCTCTTCAAC GCTGCCCTCG CTATGACAGG AAAGATGGGT GGCAAGGGGG 
     4381 CAAAAGGCGG CAAACTTGCT CACCTCCGGG CCCGGCTCAA AGAACTGGCC GCGCTGGAGG 
     4441 CGGCTGCAAA GCAGCAAGAG TTGGTGGAAC AGGCCAAGAG CTCGCAAGAC GTCAAGGGAG 
     4501 AGGAAGGCTC CGCCGCCCCA GACCAGCACA CGCACCCAAA GGAGGCCGCC ACCGACCCAC 
     4561 CCGCGCCCCG GACGCCCCCC GAGCCCCCGG GGTCTCCACC GAGCTCTCCA CCGCCTGCCT 
     4621 CCCTTGGGAG GCCGGAGGGA GAGGAGGAGG GGCCGGCCGA GCCCGAAGAG CACTCGGTGA 
     4681 GGATCTGCAT GAGCCCGGGC CCGGAGCCGG GAGAGCAGAT CCTGTCGGTG AAGATGCCGG 
     4741 AGGAAAGGGA GGAGAAGGCG GAGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4801 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4861 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4921 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4981 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     5041 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5101 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5161 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5221 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5281 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5341 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5401 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5461 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5521 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5581 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5641 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5701 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5761 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5821 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5881 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5941 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     6001 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6061 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6121 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6181 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6241 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6301 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6361 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6421 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6481 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6541 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6601 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6661 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6721 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6781 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6841 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6901 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6961 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     7021 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7081 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7141 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7201 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7261 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7321 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7381 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7441 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7501 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7561 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7621 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7681 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7741 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7801 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7861 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7921 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7981 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     8041 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8101 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8161 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8221 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8281 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8341 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8401 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8461 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8521 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8581 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//