LOCUS       RC240072                8601 bp    DNA     circular  SYN 
DEFINITION  ERBB2 (myc-DDK-tagged) - Human erb-b2 receptor tyrosine kinase 2 (ERBB2), transcript variant 3
ACCESSION   NM_001289936
VERSION     NM_001289936.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4758..4849 
                     /label="Myc-DDK"  
     misc_feature    4879..5464 
                     /label="PolyA signal"  
     misc_feature    4936..4955 
                     /label="Restriction Enzyme"  
     misc_feature    5740..6412 
                     /label="ColE1"  
     misc_feature    6842..7636 
                     /label="Kan/Neo"  
     misc_feature    7671..7973 
                     /label="SV40 ori"  
     misc_feature    8059..8536 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4740..4746 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4740 
                     /label="ERBB2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCCGGGGG TCCTGGAAGC CACAAGTGTG CACCGGCACA GACATGAAGC 
     1081 TGCGGCTCCC TGCCAGTCCC GAGACCCACC TGGACATGCT CCGCCACCTC TACCAGGGCT 
     1141 GCCAGGTGGT GCAGGGAAAC CTGGAACTCA CCTACCTGCC CACCAATGCC AGCCTGTCCT 
     1201 TCCTGCAGGA TATCCAGGAG GTGCAGGGCT ACGTGCTCAT CGCTCACAAC CAAGTGAGGC 
     1261 AGGTCCCACT GCAGAGGCTG CGGATTGTGC GAGGCACCCA GCTCTTTGAG GACAACTATG 
     1321 CCCTGGCCGT GCTAGACAAT GGAGACCCGC TGAACAATAC CACCCCTGTC ACAGGGGCCT 
     1381 CCCCAGGAGG CCTGCGGGAG CTGCAGCTTC GAAGCCTCAC AGAGATCTTG AAAGGAGGGG 
     1441 TCTTGATCCA GCGGAACCCC CAGCTCTGCT ACCAGGACAC GATTTTGTGG AAGGACATCT 
     1501 TCCACAAGAA CAACCAGCTG GCTCTCACAC TGATAGACAC CAACCGCTCT CGGGCCTGCC 
     1561 ACCCCTGTTC TCCGATGTGT AAGGGCTCCC GCTGCTGGGG AGAGAGTTCT GAGGATTGTC 
     1621 AGAGCCTGAC GCGCACTGTC TGTGCCGGTG GCTGTGCCCG CTGCAAGGGG CCACTGCCCA 
     1681 CTGACTGCTG CCATGAGCAG TGTGCTGCCG GCTGCACGGG CCCCAAGCAC TCTGACTGCC 
     1741 TGGCCTGCCT CCACTTCAAC CACAGTGGCA TCTGTGAGCT GCACTGCCCA GCCCTGGTCA 
     1801 CCTACAACAC AGACACGTTT GAGTCCATGC CCAATCCCGA GGGCCGGTAT ACATTCGGCG 
     1861 CCAGCTGTGT GACTGCCTGT CCCTACAACT ACCTTTCTAC GGACGTGGGA TCCTGCACCC 
     1921 TCGTCTGCCC CCTGCACAAC CAAGAGGTGA CAGCAGAGGA TGGAACACAG CGGTGTGAGA 
     1981 AGTGCAGCAA GCCCTGTGCC CGAGTGTGCT ATGGTCTGGG CATGGAGCAC TTGCGAGAGG 
     2041 TGAGGGCAGT TACCAGTGCC AATATCCAGG AGTTTGCTGG CTGCAAGAAG ATCTTTGGGA 
     2101 GCCTGGCATT TCTGCCGGAG AGCTTTGATG GGGACCCAGC CTCCAACACT GCCCCGCTCC 
     2161 AGCCAGAGCA GCTCCAAGTG TTTGAGACTC TGGAAGAGAT CACAGGTTAC CTATACATCT 
     2221 CAGCATGGCC GGACAGCCTG CCTGACCTCA GCGTCTTCCA GAACCTGCAA GTAATCCGGG 
     2281 GACGAATTCT GCACAATGGC GCCTACTCGC TGACCCTGCA AGGGCTGGGC ATCAGCTGGC 
     2341 TGGGGCTGCG CTCACTGAGG GAACTGGGCA GTGGACTGGC CCTCATCCAC CATAACACCC 
     2401 ACCTCTGCTT CGTGCACACG GTGCCCTGGG ACCAGCTCTT TCGGAACCCG CACCAAGCTC 
     2461 TGCTCCACAC TGCCAACCGG CCAGAGGACG AGTGTGTGGG CGAGGGCCTG GCCTGCCACC 
     2521 AGCTGTGCGC CCGAGGGCAC TGCTGGGGTC CAGGGCCCAC CCAGTGTGTC AACTGCAGCC 
     2581 AGTTCCTTCG GGGCCAGGAG TGCGTGGAGG AATGCCGAGT ACTGCAGGGG CTCCCCAGGG 
     2641 AGTATGTGAA TGCCAGGCAC TGTTTGCCGT GCCACCCTGA GTGTCAGCCC CAGAATGGCT 
     2701 CAGTGACCTG TTTTGGACCG GAGGCTGACC AGTGTGTGGC CTGTGCCCAC TATAAGGACC 
     2761 CTCCCTTCTG CGTGGCCCGC TGCCCCAGCG GTGTGAAACC TGACCTCTCC TACATGCCCA 
     2821 TCTGGAAGTT TCCAGATGAG GAGGGCGCAT GCCAGCCTTG CCCCATCAAC TGCACCCACT 
     2881 CCTGTGTGGA CCTGGATGAC AAGGGCTGCC CCGCCGAGCA GAGAGCCAGC CCTCTGACGT 
     2941 CCATCATCTC TGCGGTGGTT GGCATTCTGC TGGTCGTGGT CTTGGGGGTG GTCTTTGGGA 
     3001 TCCTCATCAA GCGACGGCAG CAGAAGATCC GGAAGTACAC GATGCGGAGA CTGCTGCAGG 
     3061 AAACGGAGCT GGTGGAGCCG CTGACACCTA GCGGAGCGAT GCCCAACCAG GCGCAGATGC 
     3121 GGATCCTGAA AGAGACGGAG CTGAGGAAGG TGAAGGTGCT TGGATCTGGC GCTTTTGGCA 
     3181 CAGTCTACAA GGGCATCTGG ATCCCTGATG GGGAGAATGT GAAAATTCCA GTGGCCATCA 
     3241 AAGTGTTGAG GGAAAACACA TCCCCCAAAG CCAACAAAGA AATCTTAGAC GAAGCATACG 
     3301 TGATGGCTGG TGTGGGCTCC CCATATGTCT CCCGCCTTCT GGGCATCTGC CTGACATCCA 
     3361 CGGTGCAGCT GGTGACACAG CTTATGCCCT ATGGCTGCCT CTTAGACCAT GTCCGGGAAA 
     3421 ACCGCGGACG CCTGGGCTCC CAGGACCTGC TGAACTGGTG TATGCAGATT GCCAAGGGGA 
     3481 TGAGCTACCT GGAGGATGTG CGGCTCGTAC ACAGGGACTT GGCCGCTCGG AACGTGCTGG 
     3541 TCAAGAGTCC CAACCATGTC AAAATTACAG ACTTCGGGCT GGCTCGGCTG CTGGACATTG 
     3601 ACGAGACAGA GTACCATGCA GATGGGGGCA AGGTGCCCAT CAAGTGGATG GCGCTGGAGT 
     3661 CCATTCTCCG CCGGCGGTTC ACCCACCAGA GTGATGTGTG GAGTTATGGT GTGACTGTGT 
     3721 GGGAGCTGAT GACTTTTGGG GCCAAACCTT ACGATGGGAT CCCAGCCCGG GAGATCCCTG 
     3781 ACCTGCTGGA AAAGGGGGAG CGGCTGCCCC AGCCCCCCAT CTGCACCATT GATGTCTACA 
     3841 TGATCATGGT CAAATGTTGG ATGATTGACT CTGAATGTCG GCCAAGATTC CGGGAGTTGG 
     3901 TGTCTGAATT CTCCCGCATG GCCAGGGACC CCCAGCGCTT TGTGGTCATC CAGAATGAGG 
     3961 ACTTGGGCCC AGCCAGTCCC TTGGACAGCA CCTTCTACCG CTCACTGCTG GAGGACGATG 
     4021 ACATGGGGGA CCTGGTGGAT GCTGAGGAGT ATCTGGTACC CCAGCAGGGC TTCTTCTGTC 
     4081 CAGACCCTGC CCCGGGCGCT GGGGGCATGG TCCACCACAG GCACCGCAGC TCATCTACCA 
     4141 GGAGTGGCGG TGGGGACCTG ACACTAGGGC TGGAGCCCTC TGAAGAGGAG GCCCCCAGGT 
     4201 CTCCACTGGC ACCCTCCGAA GGGGCTGGCT CCGATGTATT TGATGGTGAC CTGGGAATGG 
     4261 GGGCAGCCAA GGGGCTGCAA AGCCTCCCCA CACATGACCC CAGCCCTCTA CAGCGGTACA 
     4321 GTGAGGACCC CACAGTACCC CTGCCCTCTG AGACTGATGG CTACGTTGCC CCCCTGACCT 
     4381 GCAGCCCCCA GCCTGAATAT GTGAACCAGC CAGATGTTCG GCCCCAGCCC CCTTCGCCCC 
     4441 GAGAGGGCCC TCTGCCTGCT GCCCGACCTG CTGGTGCCAC TCTGGAAAGG CCCAAGACTC 
     4501 TCTCCCCAGG GAAGAATGGG GTCGTCAAAG ACGTTTTTGC CTTTGGGGGT GCCGTGGAGA 
     4561 ACCCCGAGTA CTTGACACCC CAGGGAGGAG CTGCCCCTCA GCCCCACCCT CCTCCTGCCT 
     4621 TCAGCCCAGC CTTCGACAAC CTCTATTACT GGGACCAGGA CCCACCAGAG CGGGGGGCTC 
     4681 CACCCAGCAC CTTCAAAGGG ACACCTACGG CAGAGAACCC AGAGTACCTG GGTCTGGACG 
     4741 TGCCAGTGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4801 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4861 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4921 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4981 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     5041 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5101 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5161 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5221 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5281 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5341 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5401 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5461 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5521 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5581 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5641 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5701 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5761 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5821 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5881 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5941 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     6001 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6061 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6121 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6181 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6241 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6301 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6361 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6421 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6481 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6541 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6601 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6661 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6721 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6781 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6841 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6901 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6961 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     7021 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7081 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7141 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7201 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7261 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7321 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7381 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7441 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7501 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7561 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7621 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7681 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7741 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7801 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7861 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7921 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7981 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     8041 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8101 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8161 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8221 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8281 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8341 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8401 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8461 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8521 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8581 CAAAAATTTA ACGCGAATTT T
//