LOCUS       RC240070                8595 bp    DNA     circular  SYN 
DEFINITION  AMBRA1 (myc-DDK-tagged) - Human autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant 4
ACCESSION   NM_001300731
VERSION     NM_001300731.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4752..4843 
                     /label="Myc-DDK"  
     misc_feature    4873..5458 
                     /label="PolyA signal"  
     misc_feature    4930..4949 
                     /label="Restriction Enzyme"  
     misc_feature    5734..6406 
                     /label="ColE1"  
     misc_feature    6836..7630 
                     /label="Kan/Neo"  
     misc_feature    7665..7967 
                     /label="SV40 ori"  
     misc_feature    8053..8530 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4734..4740 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4734 
                     /label="AMBRA1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGGTTGTC CCAGAAAAGA ATGCTGTCCG GATACTCTGG GGGCGAGAAC 
     1081 GGGGTGCTCG GGCCATGGGA GCTCAGCGGC TTCTGCAGGA GCTGGTAGAA GATAAAACCC 
     1141 GGTGGATGAA ATGGGAGGGC AAGAGAGTAG AACTGCCGGA TAGTCCACGC TCTACCTTCT 
     1201 TATTGGCCTT CAGCCCAGAC AGGACTCTCT TAGCCTCCAC CCATGTGAAC CATAATATCT 
     1261 ATATTACGGA GGTGAAGACT GGCAAGTGTG TTCATTCCCT GATTGGACAC CGCCGTACTC 
     1321 CATGGTGTGT CACTTTTCAT CCCACCATCT CAGGCCTTAT TGCTTCTGGC TGCCTAGATG 
     1381 GGGAGGTTAG GATTTGGGAT TTACACGGTG GCAGTGAAAG CTGGTTCACA GATAGCAACA 
     1441 ATGCCATTGC CTCCCTGGCT TTCCACCCTA CGGCTCAGCT CCTGCTGATT GCCACTGCCA 
     1501 ATGAGATCCA CTTCTGGGAC TGGAGTCGAC GGGAACCCTT TGCTGTGGTG AAGACAGCTA 
     1561 GTGAGATGGA ACGGGTCCGT CTGGTGAGAT TTGATCCACT TGGACACTAC TTACTCACAG 
     1621 CAATTGTTAA CCCCTCTAAT CAACAGGGTG ATGACGAACC AGAGATCCCC ATAGATGGAA 
     1681 CAGAATTATC CCACTACCGT CAGCGTGCCC TCCTGCAATC ACAGCCAGTT CGCCGGACGC 
     1741 CTCTCCTCCA CAATTTCCTG CACATGCTGT CCTCCCGCTC TTCTGGCATC CAGGTGGGAG 
     1801 AGCAAAGCAC AGTGCAAGAT TCTGCTACCC CCTCACCCCC ACCGCCTCCC CCTCAGCCCT 
     1861 CCACGGAGCG CCCCAGGACT TCCGCTTACA TCAGGCTCCG ACAGCGGGTC AGTTACCCCA 
     1921 CAGCTGAGTG CTGCCAGCAC CTTGGGATCC TGTGCCTTTG CAGCCGCTGC TCTGGCACTC 
     1981 GAGTTCCTTC CCTCTTGCCA CACCAGGACA GTGTCCCCCC TGCTTCTGCC AGAGCTACTA 
     2041 CCCCTTCCTT TTCTTTTGTA CAGACCGAGC CCTTCCATCC CCCGGAGCAG GCCTCGTCAA 
     2101 CGCAGCAGGA CCAGGGCCTC CTGAACCGGC CGTCTGCCTT CAGTACAGTC CAGAGCAGCA 
     2161 CTGCCGGCAA CACGCTCCGC AACCTCAGTC TGGGTCCTAC CCGCCGCTCT TTGGGAGGGC 
     2221 CTCTGTCTAG CCACCCTTCT AGGTATCACC GAGAAATAGC TCCTGGGTTG ACAGGATCTG 
     2281 AGTGGACCCG GACAGTACTC AGTCTGAACT CCCGCTCTGA GGCGGAATCC ATGCCCCCGC 
     2341 CCAGAACCAG TGCCTCTTCG GTGAGTTTGC TGTCTGTGCT GAGACAGCAG GAAGGTGGCT 
     2401 CTCAGGCATC TGTGTACACT TCAGCCACAG AAGGGAGGGG TTTTCCGGCA TCAGGGTTGG 
     2461 CAACTGAGTC AGATGGAGGG AATGGCTCCA GCCAAAACAA CTCGGGCAGC ATTCGCCATG 
     2521 AGCTTCAGTG TGACCTGAGA CGCTTCTTTC TGGAGTATGA CCGGCTTCAG GAGCTGGATC 
     2581 AGAGCCTGAG TGGGGAAGCT CCCCAGACCC AACAGGCCCA GGAAATGCTC AACAATAACA 
     2641 TTGAATCTGA GAGGCCAGGC CCTTCCCACC AGCCCACCCC ACACAGCAGT GAGAACAACT 
     2701 CCAACCTGTC CCGTGGCCAC CTGAATCGCT GTCGTGCTTG CCACAATCTC CTGACCTTCA 
     2761 ACAACGATAC CCTGCGCTGG GAAAGAACCA CACCTAACTA CTCCTCTGGC GAGGCTAGTT 
     2821 CCTCTTGGCA GGTCCCCAGC TCCTTTGAGA GTGTGCCATC AAGTGGCAGC CAGTTGCCAC 
     2881 CTCTCGAGCG GACTGAGGGC CAAACGCCCA GCTCCAGCAG GCTGGAGTTG AGCAGCTCTG 
     2941 CTAGTCCGCA GGAGGAGAGG ACTGTGGGGG TGGCCTTTAA CCAGGAGACA GGCCACTGGG 
     3001 AAAGAATTTA CACCCAGTCC AGCAGATCTG GAACTGTGTC ACAGGAGGCC TTACATCAGG 
     3061 ATATGCCTGA GGAGAGCTCT GAGGAGGATT CACTCAGGAG GAGGCTGCTG GAATCTTCCC 
     3121 TCATTTCATT ATCCCGTTAT GATGGAGCAG GATCCAGAGA GCACCCAATT TACCCAGACC 
     3181 CAGCGAGGGA CAATGGTGAC AGATCCAGGC ACCGAGCTCC ACGCAATGCC CGGATGTCTG 
     3241 CACCTTCGCT TGGACGCTTT GTCCCAAGGC GTTTCTTGCT GCCTGAGTAC TTGCCTTATG 
     3301 CTGGGATTTT TCATGAACGT GGACAGCCTG GCTTGGCTAC TCACTCTTCT GTTAACAGGG 
     3361 TCCTGGCAGG GGCAGTGATC GGTGATGGAC AGTCTGCTGT GGCCAGTAAC ATTGCCAATA 
     3421 CTACCTACCG GCTCCAGTGG TGGGACTTCA CTAAGTTTGA CCTCCCTGAA ATCAGTAATG 
     3481 CTTCCGTGAA TGTGCTGGTG CAGAACTGCA AGATCTACAA TGATGCCAGC TGTGACATTT 
     3541 CTGCAGATGG CCAGCTCCTG GCAGCTTTCA TCCCCAGCAG CCAGAGGGGC TTTCCTGATG 
     3601 AAGGCATCCT GGCAGTGTAC TCCCTGGCCC CCCATAACCT GGGCGAAATG CTCTACACCA 
     3661 AGCGATTTGG TCCCAATGCC ATTTCGGTGA GCCTGTCCCC AATGGGCAGA TATGTAATGG 
     3721 TGGGCTTGGC CTCACGAAGG ATCCTGCTGC ACCCCTCCAC AGAGCACATG GTGGCCCAGG 
     3781 TCTTCAGGCT GCAACAGGCC CATGGTGGAG AGACCTCCAT GAGGAGAGTT TTCAACGTCC 
     3841 TTTATCCCAT GCCTGCCGAC CAGCGGAGAC ATGTCAGTAT CAACTCTGCC CGTTGGCTGC 
     3901 CTGAGCCAGG GCTTGGCTTG GCCTATGGTA CTAACAAAGG AGACCTGGTG ATCTGCCGAC 
     3961 CAGAGGCCTT AAACTCTGGT GTTGAGTACT ACTGGGACCA GCTGAACGAG ACGGTCTTCA 
     4021 CTGTCCATTC CAACAGCAGG AGCAGCGAGC GGCCTGGAAC CAGCAGAGCC ACATGGAGGA 
     4081 CAGACAGAGA CATGGGGCTG ATGAATGCCA TTGGGCTTCA GCCCCGGAAC CCTGCCACCT 
     4141 CAGTGACATC TCAGGGCACC CAGACTCTGG CCCTTCAGCT GCAGAATGCC GAAACACAGA 
     4201 CTGAGAGGGA GGTGCCGGAG CCAGGGACAG CCGCCTCAGG TCCTGGTGAA GGTGAGGGTT 
     4261 CAGAGTATGG TGCCAGTGGA GAAGATGCGC TCAGCAGGAT CCAGAGGCTG ATGGCGGAGG 
     4321 GCGGCATGAC AGCCGTGGTG CAGCGGGAGC AGAGCACCAC CATGGCCTCC ATGGGCGGCT 
     4381 TCGGCAACAA CATCATCGTC AGCCACCGCA TTCACCGCAG CTCTCAGACG GGCACTGAAC 
     4441 CTGGTGCCGC CCACACCTCC TCACCCCAGC CCTCCACCTC TCGGGGACTG CTCCCAGAGG 
     4501 CCGGGCAACT GGCAGAGCGA GGCCTAAGCC CCCGGACAGC TTCCTGGGAC CAGCCTGGTA 
     4561 CCCCTGGGCG GGAGCCAACC CAGCCAACCC TGCCCTCTTC CTCCCCTGTC CCCATTCCTG 
     4621 TTTCCCTTCC CAGCGCTGAG GGACCAACCC TCCACTGCGA GTTGACCAAT AACAACCACC 
     4681 TTCTGGATGG TGGCAGCAGC AGGGGGGACG CTGCAGGCCC TAGGGGAGAA CCACGGAACA 
     4741 GGACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4801 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4861 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4921 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4981 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     5041 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5101 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5161 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5221 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5281 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5341 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5401 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5461 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5521 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5581 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5641 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5701 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5761 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5821 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5881 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5941 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     6001 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6061 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6121 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6181 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6241 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6301 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6361 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6421 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6481 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6541 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6601 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6661 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6721 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6781 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6841 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6901 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6961 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     7021 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7081 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7141 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7201 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7261 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7321 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7381 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7441 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7501 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7561 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7621 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7681 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7741 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7801 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7861 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7921 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7981 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     8041 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8101 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8161 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8221 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8281 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8341 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8401 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8461 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8521 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8581 TTTAACGCGA ATTTT
//