LOCUS       RC240068                8586 bp    DNA     circular  SYN 
DEFINITION  TBC1D4 (myc-DDK-tagged) - Human TBC1 domain family, member 4 (TBC1D4), transcript variant 3
ACCESSION   NM_001286659
VERSION     NM_001286659.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4743..4834 
                     /label="Myc-DDK"  
     misc_feature    4864..5449 
                     /label="PolyA signal"  
     misc_feature    4921..4940 
                     /label="Restriction Enzyme"  
     misc_feature    5725..6397 
                     /label="ColE1"  
     misc_feature    6827..7621 
                     /label="Kan/Neo"  
     misc_feature    7656..7958 
                     /label="SV40 ori"  
     misc_feature    8044..8521 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4725..4731 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4725 
                     /label="TBC1D4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCCGCCC AGCTGCATTC AGGATGAGCC GTTCCCGCAC CCCCTGGAGC 
     1081 CCGAGCCGGG CGTCTCAGCT CAGCCCGGCC CCGGGAAGCC AAGCGATAAG CGGTTCCGGC 
     1141 TGTGGTACGT TGGGGGGTCG TGCCTGGACC ACAGGACCAC GCTGCCTATG CTGCCCTGGC 
     1201 TCATGGCCGA GATCCGCAGG CGCAGCCAGA AGCCCGAGGC GGGCGGCTGC GGGGCGCCGG 
     1261 CGGCCCGAGA GGTGATCCTG GTGCTCAGCG CGCCCTTCCT GCGTTGCGTC CCCGCGCCGG 
     1321 GCGCTGGGGC CTCGGGGGGC ACTAGTCCGT CGGCCACGCA GCCCAACCCG GCGGTATTCA 
     1381 TCTTCGAGCA CAAGGCGCAG CATATCTCGC GCTTCATCCA CAACAGCCAC GACCTCACCT 
     1441 ACTTTGCCTA CCTGATCAAG GCGCAGCCCG ACGACCCCGA GTCGCAGATG GCCTGCCACG 
     1501 TTTTCCGCGC CACAGACCCC AGCCAGGTTC CTGATGTTAT TAGCAGCATA AGGCAATTAT 
     1561 CTAAAGCGGC CATGAAAGAG GATGCCAAAC CCAGCAAAGA TAATGAGGAC GCCTTTTACA 
     1621 ACTCTCAGAA GTTCGAAGTC CTGTACTGTG GAAAGGTGAC CGTGACCCAC AAGAAGGCCC 
     1681 CCTCAAGCCT CATCGATGAC TGCATGGAGA AGTTCAGCCT GCACGAACAG CAGCGCCTGA 
     1741 AGATCCAAGG GGAGCAGCGC GGTCCGGACC CAGGAGAGGA CCTGGCTGAC TTGGAGGTGG 
     1801 TGGTGCCCGG GTCCCCCGGA GACTGCCTGC CGGAGGAGGC TGACGGCACC GACACCCACC 
     1861 TTGGCTTACC TGCCGGGGCC AGCCAGCCTG CCCTGACCAG CTCTCGGGTC TGCTTCCCTG 
     1921 AGCGGATTTT GGAAGATTCT GGCTTTGATG AGCAGCAGGA GTTTCGGTCT CGGTGCAGCA 
     1981 GTGTCACCGG CGTGCAACGG AGAGTTCACG AGGGCAGCCA GAAATCCCAG CCGCGACGGA 
     2041 GACACGCGAG CGCACCCAGT CACGTCCAGC CCTCGGACTC GGAGAAGAAC AGGACCATGC 
     2101 TCTTCCAGGT TGGGCGATTT GAGATTAACC TTATCAGTCC AGACACTAAA TCAGTTGTGC 
     2161 TAGAAAAGAA TTTTAAAGAT ATCTCCTCTT GTTCTCAGGG TATAAAGCAT GTGGATCACT 
     2221 TTGGCTTTAT CTGCCGGGAG TCTCCAGAGC CTGGACTTAG CCAGTATATT TGTTATGTAT 
     2281 TCCAGTGTGC CAGCGAATCT CTGGTTGATG AGGTAATGCT GACTCTGAAA CAGGCCTTCA 
     2341 GTACGGCGGC TGCCCTGCAG AGTGCCAAGA CGCAGATTAA ACTGTGTGAG GCCTGCCCGA 
     2401 TGCACTCTTT GCATAAGCTC TGTGAAAGGA TTGAAGGTCT CTACCCACCA AGAGCCAAGC 
     2461 TGGTGATACA GAGGCATCTC TCATCACTGA CAGATAATGA GCAAGCTGAC ATCTTTGAAA 
     2521 GAGTTCAGAA AATGAAGCCA GTCAGTGACC AGGAAGAAAA TGAACTTGTG ATTTTACACC 
     2581 TGAGGCAGCT GTGTGAAGCC AAGCAGAAGA CACACGTGCA CATCGGGGAA GGCCCTTCTA 
     2641 CTATTTCAAA TAGTACAATC CCAGAAAATG CAACAAGCAG TGGAAGGTTC AAACTTGACA 
     2701 TTCTGAAAAA TAAAGCTAAG AGATCCTTAA CTAGCTCCCT GGAAAATATC TTCTCAAGGG 
     2761 GAGCTAACAG AATGAGAGGT CGGCTTGGAA GTGTGGACAG TTTTGAACGG TCCAACAGTC 
     2821 TTGCTTCAGA GAAGGACTAC TCACCAGGGG ATTCTCCACC AGGGACACCG CCAGCGTCCC 
     2881 CACCGTCCTC AGCTTGGCAA ACGTTTCCCG AAGAGGATTC CGACTCCCCG CAGTTTCGAA 
     2941 GACGGGCACA CACGTTCAGC CACCCACCTT CAAGCACAAA GAGAAAGCTG AATTTGCAGG 
     3001 ATGGGAGGGC TCAGGGTGTG CGTTCCCCTC TGCTGAGGCA GAGCTCCAGT GAACAGTGCA 
     3061 GTGATGGAGA AGGGAGAAAA AGGACCTCAT CTACCTGCAG CAATGAGTCC CTAAGTGTGG 
     3121 GAGGAACCTC TGTCACTCCT CGCCGGATCT CCTGGCGGCA GCGCATTTTC CTCAGGGTTG 
     3181 CTTCTCCCAT GAACAAATCT CCCTCAGCAA TGCAACAGCA AGATGGATTG GACAGGAACG 
     3241 AGCTGCTGCC ACTGTCCCCC CTCTCTCCAA CCATGGAGGA GGAACCGCTG GTTGTATTCC 
     3301 TGTCTGGGGA GGATGACCCA GAAAAGATTG AAGAAAGAAA GAAATCAAAA GAACTGAGGA 
     3361 GCTTGTGGAG AAAAGCTATA CACCAACAAA TCTTGTTACT TCGAATGGAA AAAGAAAACC 
     3421 AGAAACTTGA AGCAAGCAGA GATGAACTCC AGTCCAGAAA AGTTAAATTA GACTATGAAG 
     3481 AAGTTGGTGC ATGTCAGAAA GAGGTCTTAA TAACTTGGGA TAAGAAGTTG TTAAACTGCA 
     3541 GAGCTAAAAT CAGATGTGAT ATGGAAGATA TTCATACTCT TCTTAAAGAA GGAGTTCCCA 
     3601 AAAGTCGACG AGGAGAAATT TGGCAGTTTC TGGCTTTACA GTACCGACTC AGACACAGAT 
     3661 TGCCTAATAA ACAACAGCCT CCTGACATAT CCTATAAGGA ACTTTTGAAG CAGCTCACTG 
     3721 CTCAGCAGCA TGCGATTCTC GTGGATTTAG GAAGGACGTT TCCTACTCAC CCTTACTTTT 
     3781 CAGTACAGCT TGGGCCAGGA CAGCTGTCAC TGTTTAACCT CCTGAAAGCC TATTCTTTGC 
     3841 TGGACAAAGA AGTGGGATAC TGTCAGGGGA TCAGCTTTGT GGCTGGAGTC CTGCTTCTGC 
     3901 ACATGAGTGA AGAGCAAGCC TTTGAAATGC TGAAATTCCT CATGTATGAC CTCGGCTTCC 
     3961 GCAAGCAGTA CAGACCTGAC ATGATGTCGC TGCAGATTCA AATGTACCAG CTGTCCAGGC 
     4021 TCCTTCATGA CTATCACAGA GATCTCTACA ATCACCTTGA AGAAAATGAA ATCAGCCCCA 
     4081 GTCTTTATGC TGCCCCCTGG TTCCTCACAT TGTTTGCCTC TCAGTTTTCA TTAGGATTTG 
     4141 TAGCCAGAGT TTTTGATATT ATTTTTCTTC AGGGAACTGA AGTTATATTC AAGGTTGCAC 
     4201 TCAGCCTACT GAGCAGCCAA GAGACACTTA TAATGGAATG TGAGAGCTTT GAAAATATTG 
     4261 TTGAGTTTCT TAAAAACACG CTACCTGATA TGAATACCTC TGAAATGGAA AAAATTATTA 
     4321 CCCAGGTTTT TGAGATGGAT ATTTCTAAGC AGTTGCATGC CTATGAGGTG GAATATCATG 
     4381 TGCTACAGGA TGAGCTTCAG GAATCTTCAT ATTCCTGTGA GGATAGTGAA ACTTTGGAGA 
     4441 AGCTGGAGAG GGCCAATAGC CAACTGAAAA GACAAAACAT GGACCTCCTA GAAAAATTAC 
     4501 AGGTAGCTCA TACTAAAATC CAGGCCTTGG AATCAAACCT GGAAAATCTT TTGACGAGAG 
     4561 AGACCAAAAT GAAGTCTTTA ATCCGGACCC TGGAACAAGA AAAAATGGCT TATCAAAAGA 
     4621 CAGTGGAGCA ACTCCGGAAG CTGCTGCCCG CGGATGCTCT AGTCAATTGT GACCTGTTGC 
     4681 TGAGAGACCT AAACTGCAAC CCTAACAACA AAGCCAAGAT AGGAAATAAG CCAACGCGTA 
     4741 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4801 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4861 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4921 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4981 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5041 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5101 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5161 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5221 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5281 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5341 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5401 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5461 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5521 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5581 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5641 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5701 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5761 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5821 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5881 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5941 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6001 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6061 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6121 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6181 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6241 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6301 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6361 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6421 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6481 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6541 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6601 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6661 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6721 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6781 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6841 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6901 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6961 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7021 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7081 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7141 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7201 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7261 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7321 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7381 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7441 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7501 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7561 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7621 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7681 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7741 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7801 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7861 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7921 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7981 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8041 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8101 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8161 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8221 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8281 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8341 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8401 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8461 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8521 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8581 AATTTT
//