LOCUS       RC240065                8580 bp    DNA     circular  SYN 
DEFINITION  EHMT2 (myc-DDK-tagged) - Human euchromatic histone-lysine N-methyltransferase 2 (EHMT2), transcript variant 1
ACCESSION   NM_001289413
VERSION     NM_001289413.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4737..4828 
                     /label="Myc-DDK"  
     misc_feature    4858..5443 
                     /label="PolyA signal"  
     misc_feature    4915..4934 
                     /label="Restriction Enzyme"  
     misc_feature    5719..6391 
                     /label="ColE1"  
     misc_feature    6821..7615 
                     /label="Kan/Neo"  
     misc_feature    7650..7952 
                     /label="SV40 ori"  
     misc_feature    8038..8515 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4719..4725 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4719 
                     /label="EHMT2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGGGGTCTA CCGAGAGGGA GGGGGTTGAT GCGGGCCCGG GGGAGGGGTC 
     1081 GTGCGGCCCC TCCGGGCAGC CGAGGCCGCG GAAGGGGGGG GCCCCACAGA GGAAGAGGTA 
     1141 GGCCCCGGAG CCTACTCTCT CTTCCCAGGG CCCAGGCATC CTGGACCCCC CAACTCTCTA 
     1201 CTGGGCTGAC CAGCCCTCCT GTCCCTTGTC TCCCCTCCCA GGGGGAGGCC CCCGCTGAGA 
     1261 TGGGGGCGCT GCTGCTGGAG AAGGAAACCA GAGGAGCCAC CGAGAGAGTT CATGGCTCTT 
     1321 TGGGGGACAC CCCTCGTAGT GAAGAAACCC TGCCCAAGGC CACCCCCGAC TCCCTGGAGC 
     1381 CTGCTGGCCC CTCATCTCCA GCCTCTGTCA CTGTCACTGT TGGTGATGAG GGGGCTGACA 
     1441 CCCCTGTAGG GGCTACACCA CTCATTGGGG ATGAATCTGA GAATCTTGAG GGAGATGGGG 
     1501 ACCTCCGTGG GGGCCGGATC CTGCTGGGCC ATGCCACAAA GTCATTCCCC TCTTCCCCCA 
     1561 GCAAGGGGGG TTCCTGTCCT AGCCGGGCCA AGATGTCAAT GACAGGGGCG GGAAAATCAC 
     1621 CTCCATCTGT CCAGAGTTTG GCTATGAGGC TACTGAGTAT GCCAGGAGCC CAGGGAGCTG 
     1681 CAGCAGCAGG GTCTGAACCC CCTCCAGCCA CCACGAGCCC AGAGGGACAG CCCAAGGTCC 
     1741 ACCGAGCCCG CAAAACCATG TCCAAACCAG GAAATGGACA GCCCCCGGTC CCTGAGAAGC 
     1801 GGCCCCCTGA AATACAGCAT TTCCGCATGA GTGATGATGT CCACTCACTG GGAAAGGTGA 
     1861 CCTCAGATCT GGCCAAAAGG AGGAAGCTGA ACTCAGGAGG TGGCCTGTCA GAGGAGTTAG 
     1921 GTTCTGCCCG GCGTTCAGGA GAAGTGACCC TGACGAAAGG GGACCCCGGG TCCCTGGAGG 
     1981 AGTGGGAGAC GGTGGTGGGT GATGACTTCA GTCTCTACTA TGATTCCTAC TCTGTGGATG 
     2041 AGCGCGTGGA CTCCGACAGC AAGTCTGAAG TTGAAGCTCT AACTGAACAA CTAAGTGAAG 
     2101 AGGAGGAGGA GGAAGAGGAG GAAGAAGAAG AAGAGGAAGA GGAGGAGGAA GAGGAAGAAG 
     2161 AAGAGGAAGA TGAGGAGTCA GGGAATCAGT CAGATAGGAG TGGTTCCAGT GGCCGGCGCA 
     2221 AGGCCAAGAA GAAATGGCGA AAAGACAGCC CATGGGTGAA GCCGTCTCGG AAACGGCGCA 
     2281 AGCGGGAGCC TCCGCGGGCC AAGGAGCCAC GAGGGGTGTC CAATGACACA TCTTCGCTGG 
     2341 AGACAGAGCG AGGGTTTGAG GAGTTGCCCC TGTGCAGCTG CCGCATGGAG GCACCCAAGA 
     2401 TTGACCGCAT CAGCGAGAGG GCGGGGCACA AGTGCATGGC CACTGAGAGT GTGGACGGAG 
     2461 AGCTGTCAGG CTGCAATGCC GCCATCCTCA AGCGGGAGAC CATGAGGCCA TCCAGCCGTG 
     2521 TGGCCCTGAT GGTGCTCTGT GAGACCCACC GCGCCCGCAT GGTCAAACAC CACTGCTGCC 
     2581 CGGGCTGCGG CTACTTCTGC ACGGCGGGCA CCTTCCTGGA GTGCCACCCT GACTTCCGTG 
     2641 TGGCCCACCG CTTCCACAAG GCCTGTGTGT CTCAGCTGAA TGGGATGGTC TTCTGTCCCC 
     2701 ACTGTGGGGA GGATGCTTCT GAAGCTCAAG AGGTGACCAT CCCCCGGGGT GACGGGGTGA 
     2761 CCCCACCGGC CGGCACTGCA GCTCCTGCAC CCCCACCCCT GTCCCAGGAT GTCCCCGGGA 
     2821 GAGCAGACAC TTCTCAGCCC AGTGCCCGGA TGCGAGGGCA TGGGGAACCC CGGCGCCCGC 
     2881 CCTGCGATCC CCTGGCTGAC ACCATTGACA GCTCAGGGCC CTCCCTGACC CTGCCCAATG 
     2941 GGGGCTGCCT TTCAGCCGTG GGGCTGCCAC TGGGGCCAGG CCGGGAGGCC CTGGAAAAGG 
     3001 CCCTGGTCAT CCAGGAGTCA GAGAGGCGGA AGAAGCTCCG TTTCCACCCT CGGCAGTTGT 
     3061 ACCTGTCCGT GAAGCAGGGC GAGCTGCAGA AGGTGATCCT GATGCTGTTG GACAACCTGG 
     3121 ACCCCAACTT CCAGAGCGAC CAGCAGAGCA AGCGCACGCC CCTGCATGCA GCCGCCCAGA 
     3181 AGGGCTCCGT GGAGATCTGC CATGTGCTGC TGCAGGCTGG AGCCAACATA AATGCAGTGG 
     3241 ACAAACAGCA GCGGACGCCA CTGATGGAGG CCGTGGTGAA CAACCACCTG GAGGTAGCCC 
     3301 GTTACATGGT GCAGCGTGGT GGCTGTGTCT ATAGCAAGGA GGAGGACGGT TCCACCTGCC 
     3361 TCCACCACGC AGCCAAAATC GGGAACTTGG AGATGGTCAG CCTGCTGCTG AGCACAGGAC 
     3421 AGGTGGACGT CAACGCCCAG GACAGTGGGG GGTGGACGCC CATCATCTGG GCTGCAGAGC 
     3481 ACAAGCACAT CGAGGTGATC CGCATGCTAC TGACGCGGGG CGCCGACGTC ACCCTCACTG 
     3541 ACAACGAGGA GAACATCTGC CTGCACTGGG CCTCCTTCAC GGGCAGCGCC GCCATCGCCG 
     3601 AAGTCCTTCT GAATGCGCGC TGTGACCTCC ATGCTGTCAA CTACCATGGG GACACCCCCC 
     3661 TGCACATCGC AGCTCGGGAG AGCTACCATG ACTGCGTGCT GTTATTCCTG TCACGTGGGG 
     3721 CCAACCCTGA GCTGCGGAAC AAAGAGGGGG ACACAGCATG GGACCTGACT CCCGAGCGCT 
     3781 CCGACGTGTG GTTTGCGCTT CAACTCAACC GCAAGCTCCG ACTTGGGGTG GGAAATCGGG 
     3841 CCATCCGCAC AGAGAAGATC ATCTGCCGGG ACGTGGCTCG GGGCTATGAG AACGTGCCCA 
     3901 TTCCCTGTGT CAACGGTGTG GATGGGGAGC CCTGCCCTGA GGATTACAAG TACATCTCAG 
     3961 AGAACTGCGA GACGTCCACC ATGAACATCG ATCGCAACAT CACCCACCTG CAGCACTGCA 
     4021 CGTGTGTGGA CGACTGCTCT AGCTCCAACT GCCTGTGCGG CCAGCTCAGC ATCCGGTGCT 
     4081 GGTATGACAA GGATGGGCGA TTGCTCCAGG AATTTAACAA GATTGAGCCT CCGCTGATTT 
     4141 TCGAGTGTAA CCAGGCGTGC TCATGCTGGA GAAACTGCAA GAACCGGGTC GTACAGAGTG 
     4201 GCATCAAGGT GCGGCTACAG CTCTACCGAA CAGCCAAGAT GGGCTGGGGG GTCCGCGCCC 
     4261 TGCAGACCAT CCCACAGGGG ACCTTCATCT GCGAGTATGT CGGGGAGCTG ATCTCTGATG 
     4321 CTGAGGCTGA TGTGAGAGAG GATGATTCTT ACCTCTTCGA CTTAGACAAC AAGGATGGAG 
     4381 AGGTGTACTG CATAGATGCC CGTTACTATG GCAACATCAG CCGCTTCATC AACCACCTGT 
     4441 GTGACCCCAA CATCATTCCC GTCCGGGTCT TCATGCTGCA CCAAGACCTG CGATTTCCAC 
     4501 GCATCGCCTT CTTCAGTTCC CGAGACATCC GGACTGGGGA GGAGCTAGGG TTTGACTATG 
     4561 GCGACCGCTT CTGGGACATC AAAAGCAAAT ATTTCACCTG CCAATGTGGC TCTGAGAAGT 
     4621 GCAAGCACTC AGCCGAAGCC ATTGCCCTGG AGCAGAGCCG TCTGGCCCGC CTGGACCCAC 
     4681 ACCCTGAGCT GCTGCCCGAG CTCGGCTCCC TGCCCCCTGT CAACACAACG CGTACGCGGC 
     4741 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4801 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4861 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4921 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4981 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     5041 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     5101 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5161 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5221 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5281 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5341 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5401 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5461 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5521 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5581 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5641 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5701 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5761 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5821 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5881 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5941 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     6001 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     6061 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6121 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6181 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6241 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6301 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6361 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6421 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6481 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6541 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6601 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6661 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6721 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6781 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6841 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6901 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6961 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     7021 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     7081 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7141 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7201 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7261 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7321 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7381 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7441 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7501 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7561 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7621 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7681 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7741 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7801 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7861 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7921 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7981 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     8041 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     8101 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8161 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8221 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8281 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8341 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8401 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8461 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8521 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//