LOCUS       RC240060                8571 bp    DNA     circular  SYN 
DEFINITION  MSH6 (myc-DDK-tagged) - Human mutS homolog 6 (MSH6), transcript variant 2
ACCESSION   NM_001281492
VERSION     NM_001281492.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4728..4819 
                     /label="Myc-DDK"  
     misc_feature    4849..5434 
                     /label="PolyA signal"  
     misc_feature    4906..4925 
                     /label="Restriction Enzyme"  
     misc_feature    5710..6382 
                     /label="ColE1"  
     misc_feature    6812..7606 
                     /label="Kan/Neo"  
     misc_feature    7641..7943 
                     /label="SV40 ori"  
     misc_feature    8029..8506 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4710..4716 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4710 
                     /label="MSH6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGCGACAG AGCACCCTGT ACAGCTTCTT CCCCAAGTCT CCGGCGCTGA 
     1081 GTGATGCCAA CAAGGCCTCG GCCAGGGCCT CACGCGAAGG CGGCCGTGCC GCCGCTGCCC 
     1141 CCGGGGCCTC TCCTTCCCCA GGCGGGGATG CGGCCTGGAG CGAGGCTGGG CCTGGGCCCA 
     1201 GGCCCTTGGC GCGCTCCGCG TCACCGCCCA AGGCGAAGAA CCTCAACGGA GGGCTGCGGA 
     1261 GATCGGTAGG CACAACTTAC GTAACAGATA AGAGTGAAGA AGATAATGAA ATTGAGAGTG 
     1321 AAGAGGAAGT ACAGCCTAAG ACACAAGGAT CTAGGCGAAG TAGCCGCCAA ATAAAAAAAC 
     1381 GAAGGGTCAT ATCAGATTCT GAGAGTGACA TTGGTGGCTC TGATGTGGAA TTTAAGCCAG 
     1441 ACACTAAGGA GGAAGGAAGC AGTGATGAAA TAAGCAGTGG AGTGGGGGAT AGTGAGAGTG 
     1501 AAGGCCTGAA CAGCCCTGTC AAAGTTGCTC GAAAGCGGAA GAGAATGGTG ACTGGAAATG 
     1561 GCTCTCTTAA AAGGAAAAGC TCTAGGAAGG AAACGCCCTC AGCCACCAAA CAAGCAACTA 
     1621 GCATTTCATC AGAAACCAAG AATACTTTGA GAGCTTTCTC TGCCCCTCAA AATTCTGAAT 
     1681 CCCAAGCCCA CGTTAGTGGA GGTGGTGATG ACAGTAGTCG CCCTACTGTT TGGTATCATG 
     1741 AAACTTTAGA ATGGCTTAAG GAGGAAAAGA GAAGAGATGA GCACAGGAGG AGGCCTGATC 
     1801 ACCCCGATTT TGATGCATCT ACACTCTATG TGCCTGAGGA TTTCCTCAAT TCTTGTACTC 
     1861 CTGGGATGAG GAAGTGGTGG CAGATTAAGT CTCAGAACTT TGATCTTGTC ATCTGTTACA 
     1921 AGGTGGGGAA ATTTTATGAG CTGTACCACA TGGATGCTCT TATTGGAGTC AGTGAACTGG 
     1981 GGCTGGTATT CATGAAAGGC AACTGGGCCC ATTCTGGCTT TCCTGAAATT GCATTTGGCC 
     2041 GTTATTCAGA TTCCCTGGTG CAGAAGGGCT ATAAAGTAGC ACGAGTGGAA CAGACTGAGA 
     2101 CTCCAGAAAT GATGGAGGCA CGATGTAGAA AGATGGCACA TATATCCAAG TATGATAGAG 
     2161 TGGTGAGGAG GGAGATCTGT AGGATCATTA CCAAGGGTAC ACAGACTTAC AGTGTGCTGG 
     2221 AAGGTGATCC CTCTGAGAAC TACAGTAAGT ATCTTCTTAG CCTCAAAGAA AAAGAGGAAG 
     2281 ATTCTTCTGG CCATACTCGT GCATATGGTG TGTGCTTTGT TGATACTTCA CTGGGAAAGT 
     2341 TTTTCATAGG TCAGTTTTCA GATGATCGCC ATTGTTCGAG ATTTAGGACT CTAGTGGCAC 
     2401 ACTATCCCCC AGTACAAGTT TTATTTGAAA AAGGAAATCT CTCAAAGGAA ACTAAAACAA 
     2461 TTCTAAAGAG TTCATTGTCC TGTTCTCTTC AGGAAGGTCT GATACCCGGC TCCCAGTTTT 
     2521 GGGATGCATC CAAAACTTTG AGAACTCTCC TTGAGGAAGA ATATTTTAGG GAAAAGCTAA 
     2581 GTGATGGCAT TGGGGTGATG TTACCCCAGG TGCTTAAAGG TATGACTTCA GAGTCTGATT 
     2641 CCATTGGGTT GACACCAGGA GAGAAAAGTG AATTGGCCCT CTCTGCTCTA GGTGGTTGTG 
     2701 TCTTCTACCT CAAAAAATGC CTTATTGATC AGGAGCTTTT ATCAATGGCT AATTTTGAAG 
     2761 AATATATTCC CTTGGATTCT GACACAGTCA GCACTACAAG ATCTGGTGCT ATCTTCACCA 
     2821 AAGCCTATCA ACGAATGGTG CTAGATGCAG TGACATTAAA CAACTTGGAG ATTTTTCTGA 
     2881 ATGGAACAAA TGGTTCTACT GAAGGAACCC TACTAGAGAG GGTTGATACT TGCCATACTC 
     2941 CTTTTGGTAA GCGGCTCCTA AAGCAATGGC TTTGTGCCCC ACTCTGTAAC CATTATGCTA 
     3001 TTAATGATCG TCTAGATGCC ATAGAAGACC TCATGGTTGT GCCTGACAAA ATCTCCGAAG 
     3061 TTGTAGAGCT TCTAAAGAAG CTTCCAGATC TTGAGAGGCT ACTCAGTAAA ATTCATAATG 
     3121 TTGGGTCTCC CCTGAAGAGT CAGAACCACC CAGACAGCAG GGCTATAATG TATGAAGAAA 
     3181 CTACATACAG CAAGAAGAAG ATTATTGATT TTCTTTCTGC TCTGGAAGGA TTCAAAGTAA 
     3241 TGTGTAAAAT TATAGGGATC ATGGAAGAAG TTGCTGATGG TTTTAAGTCT AAAATCCTTA 
     3301 AGCAGGTCAT CTCTCTGCAG ACAAAAAATC CTGAAGGTCG TTTTCCTGAT TTGACTGTAG 
     3361 AATTGAACCG ATGGGATACA GCCTTTGACC ATGAAAAGGC TCGAAAGACT GGACTTATTA 
     3421 CTCCCAAAGC AGGCTTTGAC TCTGATTATG ACCAAGCTCT TGCTGACATA AGAGAAAATG 
     3481 AACAGAGCCT CCTGGAATAC CTAGAGAAAC AGCGCAACAG AATTGGCTGT AGGACCATAG 
     3541 TCTATTGGGG GATTGGTAGG AACCGTTACC AGCTGGAAAT TCCTGAGAAT TTCACCACTC 
     3601 GCAATTTGCC AGAAGAATAC GAGTTGAAAT CTACCAAGAA GGGCTGTAAA CGATACTGGA 
     3661 CCAAAACTAT TGAAAAGAAG TTGGCTAATC TCATAAATGC TGAAGAACGG AGGGATGTAT 
     3721 CATTGAAGGA CTGCATGCGG CGACTGTTCT ATAACTTTGA TAAAAATTAC AAGGACTGGC 
     3781 AGTCTGCTGT AGAGTGTATC GCAGTGTTGG ATGTTTTACT GTGCCTGGCT AACTATAGTC 
     3841 GAGGGGGTGA TGGTCCTATG TGTCGCCCAG TAATTCTGTT GCCGGAAGAT ACCCCCCCCT 
     3901 TCTTAGAGCT TAAAGGATCA CGCCATCCTT GCATTACGAA GACTTTTTTT GGAGATGATT 
     3961 TTATTCCTAA TGACATTCTA ATAGGCTGTG AGGAAGAGGA GCAGGAAAAT GGCAAAGCCT 
     4021 ATTGTGTGCT TGTTACTGGA CCAAATATGG GGGGCAAGTC TACGCTTATG AGACAGGCTG 
     4081 GCTTATTAGC TGTAATGGCC CAGATGGGTT GTTACGTCCC TGCTGAAGTG TGCAGGCTCA 
     4141 CACCAATTGA TAGAGTGTTT ACTAGACTTG GTGCCTCAGA CAGAATAATG TCAGGTGAAA 
     4201 GTACATTTTT TGTTGAATTA AGTGAAACTG CCAGCATACT CATGCATGCA ACAGCACATT 
     4261 CTCTGGTGCT TGTGGATGAA TTAGGAAGAG GTACTGCAAC ATTTGATGGG ACGGCAATAG 
     4321 CAAATGCAGT TGTTAAAGAA CTTGCTGAGA CTATAAAATG TCGTACATTA TTTTCAACTC 
     4381 ACTACCATTC ATTAGTAGAA GATTATTCTC AAAATGTTGC TGTGCGCCTA GGACATATGG 
     4441 CATGCATGGT AGAAAATGAA TGTGAAGACC CCAGCCAGGA GACTATTACG TTCCTCTATA 
     4501 AATTCATTAA GGGAGCTTGT CCTAAAAGCT ATGGCTTTAA TGCAGCAAGG CTTGCTAATC 
     4561 TCCCAGAGGA AGTTATTCAA AAGGGACATA GAAAAGCAAG AGAATTTGAG AAGATGAATC 
     4621 AGTCACTACG ATTATTTCGG GAAGTTTGCC TGGCTAGTGA AAGGTCAACT GTAGATGCTG 
     4681 AAGCTGTCCA TAAATTGCTG ACTTTGATTA AGGAATTAAC GCGTACGCGG CCGCTCGAGC 
     4741 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4801 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4861 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4921 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4981 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     5041 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5101 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5161 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5221 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5281 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5341 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5401 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5461 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5521 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5581 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5641 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5701 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5761 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5821 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5881 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5941 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     6001 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6061 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6121 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6181 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6241 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6301 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6361 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6421 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6481 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6541 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6601 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6661 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6721 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6781 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6841 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6901 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6961 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     7021 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7081 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7141 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7201 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7261 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7321 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7381 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7441 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7501 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7561 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7621 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7681 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7741 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7801 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7861 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7921 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7981 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     8041 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8101 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8161 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8221 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8281 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8341 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8401 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8461 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8521 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//