LOCUS       RC240059                8565 bp    DNA     circular  SYN 
DEFINITION  ASTN1 (myc-DDK-tagged) - Human astrotactin 1 (ASTN1), transcript variant 3
ACCESSION   NM_001286164
VERSION     NM_001286164.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4722..4813 
                     /label="Myc-DDK"  
     misc_feature    4843..5428 
                     /label="PolyA signal"  
     misc_feature    4900..4919 
                     /label="Restriction Enzyme"  
     misc_feature    5704..6376 
                     /label="ColE1"  
     misc_feature    6806..7600 
                     /label="Kan/Neo"  
     misc_feature    7635..7937 
                     /label="SV40 ori"  
     misc_feature    8023..8500 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4704..4710 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4704 
                     /label="ASTN1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTTTAGCC GGGCTCTGCG CCCTGCTCGC CTGCTGCTGG GGGCCGGCGG 
     1081 CGGTGCTGGC CACGGCCGCC GGCGACGTGG ATCCATCCAA GGAGCTGGAG TGCAAGCTCA 
     1141 AAAGCATCAC GGTGTCGGCA CTGCCCTTCC TGCGCGAGAA CGACCTGAGC ATCATGCACA 
     1201 GCCCCTCGGC CTCGGAGCCC AAGCTCCTCT TCTCGGTGCG CAACGACTTC CCGGGAGAAA 
     1261 TGGTCGTGGT GGACGACCTG GAGAACACGG AGCTGCCCTA CTTCGTGCTG GAGATCTCAG 
     1321 GGAACACAGA GGATATCCCT TTGGTGCGCT GGAGGCAGCA GTGGCTGGAG AATGGCACTT 
     1381 TGCTTTTTCA CATTCATCAC CAAGATGGTG CCCCAAGCCT TCCTGGACAA GACCCCACTG 
     1441 AAGAACCCCA ACATGAGTCG GCAGAAGAGG AGCTGAGGAT CCTCCACATC TCAGTCATGG 
     1501 GTGGCATGAT CGCTCTGCTG CTGTCCATCT TGTGCCTGGT GATGATCCTG TATACTCGCC 
     1561 GGCGCTGGTG CAAACGCCGC CGGGTCCCGC AGCCCCAGAA GAGTGCCAGT GCTGAGGCAG 
     1621 CCAATGAGAT TCACTACATT CCTTCTGTGC TGATCGGCGG GCACGGACGG GAGAGCCTGC 
     1681 GCAATGCCCG CGTGCAGGGC CACAACTCCA GTGGCACCCT GAGCATCCGG GAGACACCTA 
     1741 TCCTGGACGG CTATGAGTAT GACATCACTG ATCTGCGCCA CCATCTGCAG AGGGAGTGCA 
     1801 TGAACGGAGG GGAGGACTTT GCCAGCCAGG TCACGCGCAC CCTCGACTCC CTGCAGGGCT 
     1861 GCAATGAAAA GTCGGGGATG GACCTCACAC CAGGAAGTGA CAATGCCAAG CTGTCACTGA 
     1921 TGAACAAGTA TAAAGATAAT ATTATAGCCA CTAGCCCTGT GGACTCCAAC CACCAGCAAG 
     1981 CCACCCTTCT CTCTCACACC TCCAGCAGCC AGAGAAAGCG GATCAACAAC AAAGCAAGAG 
     2041 CTGGTTCCGC CTTCTTGAAC CCTGAAGGGG ATTCTGGCAC AGAGGCAGAA AACGACCCCC 
     2101 AGCTGACCTT TTACACGGAT CCTTCAAGGA GCAGGAGGCG TAGTAGAGTG GGTTCTCCCC 
     2161 GAAGTCCTGT GAATAAGACC ACCTTGACCC TGATCAGCAT CACCAGCTGT GTGATTGGCC 
     2221 TCGTGTGCTC CTCTCACGTC AACTGCCCTC TCGTTGTCAA GATCACCCTG CATGTCCCTG 
     2281 AGCACCTGAT TGCTGATGGG AGCCGCTTCA TCTTGCTGGA GGGGAGCCAG CTGGATGCCA 
     2341 GTGACTGGCT GAACCCTGCC CAAGTGGTTC TCTTCTCTCA GCAGAACTCC AGCGGACCCT 
     2401 GGGCCATGGA CCTCTGTGCC CGGCGGCTCC TGGACCCCTG TGAACACCAA TGTGACCCCG 
     2461 AAACTGGGGA ATGCCTCTGC TATGAAGGCT ACATGAAGGA TCCAGTACAT AAGCACCTTT 
     2521 GCATTCGGAA CGAATGGGGG ACAAACCAGG GGCCATGGCC TTACACAATA TTTCAGCGAG 
     2581 GCTTTGACCT GGTTTTGGGA GAGCAGCCCT CTGATAAAAT ATTTAGATTC ACCTACACTC 
     2641 TTGGGGAGGG CATGTGGTTG CCCCTCAGCA AGAGCTTTGT GATTCCACCA GCCGAACTGG 
     2701 CCATCAATCC ATCAGCAAAG TGCAAGACGG ACATGACTGT GATGGAGGAT GCTGTGGAGG 
     2761 TCAGAGAGGA GCTGATGACT TCATCCTCCT TCGACAGCCT GGAGGTTCTC TTAGATTCCT 
     2821 TTGGGCCGGT GCGCGACTGC AGCAAAGATA ACGGGGGCTG CAGTAAGAAT TTCCGCTGTA 
     2881 TTTCAGATCG CAAGCTGGAC TCCACTGGTT GCGTGTGCCC ATCTGGACTC AGTCCCATGA 
     2941 AGGACAGCTC TGGCTGCTAT GACCGCCACA TCGGGGTGGA CTGTTCCGAC GGCTTCAACG 
     3001 GCGGCTGTGA GCAGCTGTGC CTCCAGCAGA TGGCGCCCTT CCCGGACGAC CCCACCTTGT 
     3061 ATAACATCCT CATGTTCTGT GGGTGCATCG AGGACTACAA GCTTGGTGTG GATGGACGCT 
     3121 CTTGCCAACT CATCACGGAG ACCTGTCCAG AGGGAAGTGA CTGTGGGGAA AGCAGGGAGC 
     3181 TTCCCATGAA CCAGACCCTC TTTGGGGAGA TGTTCTTTGG TTACAACAAC CATTCCAAGG 
     3241 AAGTGGCTGC CGGACAGGTG CTGAAAGGAA CATTCAGGCA AAACAACTTT GCTCGTGGTT 
     3301 TAGACCAGCA ACTGCCAGAT GGTCTTGTGG TGGCCACTGT GCCCCTGGAG AATCAATGCC 
     3361 TAGAGGAGAT CTCGGAGCCC ACCCCTGACC CTGACTTCCT GACTGGGATG GTGAACTTCA 
     3421 GTGAAGTGTC TGGGTACCCT GTGCTGCAGC ACTGGAAGGT CCGGTCTGTG ATGTACCACA 
     3481 TCAAACTCAA CCAAGTGGCC ATCTCTCAGG CCCTCAGCAA TGCTCTCCAC TCGCTGGATG 
     3541 GGGCTACATC TCGTGCAGAT TTTGTGGCGC TGTTGGACCA GTTCGGCAAC CATTACATCC 
     3601 AGGAAGCTAT CTACGGCTTT GAGGAGTCCT GTTCTATCTG GTACCCAAAC AAGCAGGTCC 
     3661 AGCGGCGACT CTGGCTGGAG TATGAAGACA TCAGTAAAGG CAACTCCCCA TCAGATGAGT 
     3721 CTGAGGAGCG GGAAAGAGAC CCCAAGGTGC TGACATTCCC AGAATACATC ACCAGCTTGT 
     3781 CAGACTCCGG CACCAAGCAC ATGGCGGCTG GAGTCCGCAT GGAGTGCCAC AGCAAGGGAC 
     3841 GATGCCCCTC GTCCTGCCCC CTGTGTCATG TGACATCCAG CCCTGACACC CCTGCTGAGC 
     3901 CGGTTCTGCT GGAGGTGACC AAAGCAGCCC CCATCTATGA ACTAGTGACC AACAACCAGA 
     3961 CCCAGAGGCT CTTGCAGGAG GCTACCATGA GCTCTCTCTG GTGCTCAGGG ACTGGAGATG 
     4021 TCATCGAGGA CTGGTGTCGA TGTGACTCCA CTGCTTTTGG AGCTGATGGA CTCCCCACCT 
     4081 GTGCGCCTCT CCCACAGCCT GTGCTGAGAC TCTCCACGGT TCACGAGCCC AGCAGCACTC 
     4141 TTGTGGTCCT GGAGTGGGAA CACTCAGAGC CACCAATCGG GGTGCAGATT GTAGATTACC 
     4201 TCCTCCGTCA AGAGAAAGTC ACTGACAGGA TGGACCACTC CAAAGTGGAG ACAGAAACAG 
     4261 TGCTGAGCTT TGTGGACGAC ATCATCTCTG GAGCAAAGTC TCCTTGTGCA ATGCCATCTC 
     4321 AGGTGCCGGA CAAGCAGCTC ACCACCATCT CTCTGATCAT ACGATGCCTG GAACCTGACA 
     4381 CCATTTACAT GTTCACGCTG TGGGGAGTGG ACAACACAGG ACGGCGCTCC AGGCCAAGCG 
     4441 ACGTGATCGT GAAGACCCCA TGCCCCGTGG TGGATGATGT CAAGGCTCAA GAAATAGCAG 
     4501 ACAAGATCTA CAATCTCTTC AATGGCTACA CTAGTGGGAA GGAGCAGCAG ACCGCCTACA 
     4561 ACACCCTCCT GGATCTGGGT TCCCCCACCT TACACCGGGT CCTCTACCAC TATAACCAGC 
     4621 ACTATGAGAG TTTTGGGGAA TTCACCTGGC GATGTGAGGA TGAGTTAGGT CCCAGGCTTC 
     4681 CAGCGTGTTG GGACAGTCAG AGGAGTTGTA GAACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4741 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4801 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4861 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4921 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4981 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5041 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5101 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5161 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5221 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5281 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5341 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5401 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5461 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5521 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5581 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5641 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5701 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5761 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5821 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5881 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5941 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6001 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6061 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6121 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6181 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6241 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6301 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6361 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6421 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6481 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6541 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6601 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6661 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6721 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6781 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6841 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6901 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6961 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7021 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7081 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7141 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7201 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7261 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7321 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7381 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7441 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7501 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7561 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7621 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7681 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7741 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7801 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7861 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7921 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7981 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8041 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8101 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8161 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8221 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8281 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8341 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8401 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8461 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8521 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//