LOCUS       RC240058                8562 bp    DNA     circular  SYN 
DEFINITION  CYFIP2 (myc-DDK-tagged) - Human cytoplasmic FMR1 interacting protein 2 (CYFIP2), transcript variant 4
ACCESSION   NM_001291721
VERSION     NM_001291721.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4719..4810 
                     /label="Myc-DDK"  
     misc_feature    4840..5425 
                     /label="PolyA signal"  
     misc_feature    4897..4916 
                     /label="Restriction Enzyme"  
     misc_feature    5701..6373 
                     /label="ColE1"  
     misc_feature    6803..7597 
                     /label="Kan/Neo"  
     misc_feature    7632..7934 
                     /label="SV40 ori"  
     misc_feature    8020..8497 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4701..4707 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4701 
                     /label="CYFIP2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCACGCAC GTCACCCTGG AAGATGCCCT GTCCAACGTG GACCTGCTTG 
     1081 AAGAGCTTCC CCTCCCCGAC CAGCAGCCAT GCATCGAGCC TCCACCTTCC TCCATCATGT 
     1141 ACCAGGCTAA CTTTGACACA AACTTTGAGG ACAGGAATGC ATTTGTCACG GGCATTGCAA 
     1201 GGTACATTGA GCAGGCTACA GTCCACTCCA GCATGGTGAA ATGCAACGAG CAGCCCAACC 
     1261 GAGTAGAGAT CTATGAGAAG ACAGTAGAGG TGCTGGAGCC GGAGGTCACC AAGCTCATGA 
     1321 AGTTCATGTA TTTTCAGCGC AAGGCCATCG AGCGGTTCTG CAGCGAGGTG AAGCGGCTGT 
     1381 GCCATGCCGA GCGCAGGAAG GACTTTGTCT CTGAGGCCTA CCTCCTGACC CTTGGCAAGT 
     1441 TCATCAACAT GTTTGCTGTC CTGGATGAGC TAAAGAACAT GAAGTGCAGC GTCAAGAATG 
     1501 ACCACTCTGC CTACAAGAGG GCAGCACAGT TCCTGCGGAA GATGGCAGAT CCCCAGTCTA 
     1561 TCCAGGAGTC GCAGAACCTT TCCATGTTCC TGGCCAACCA CAACAGGATC ACCCAGTGTC 
     1621 TCCACCAGCA ACTTGAAGTG ATCCCAGGCT ATGAGGAGCT GCTGGCTGAC ATTGTCAACA 
     1681 TCTGTGTGGA TTACTACGAG AACAAGATGT ACCTGACTCC CAGTGAGAAA CATATGCTCC 
     1741 TCAAGGTGAT GGGCTTTGGC CTCTACCTAA TGGATGGAAA TGTCAGTAAC ATTTACAAAC 
     1801 TGGATGCCAA GAAGAGAATT AATCTTAGCA AAATTGATAA ATTCTTTAAG CAGCTGCAGG 
     1861 TGGTGCCCCT TTTCGGCGAC ATGCAGATAG AGCTGGCCAG ATACATTAAG ACCAGTGCTC 
     1921 ACTATGAAGA GAACAAGTCC AAGTGGACGT GCACCCAGAG CAGCATCAGC CCCCAGTACA 
     1981 ATATCTGCGA GCAGATGGTT CAGATCCGGG ATGACCACAT CCGCTTCATC TCCGAGCTCG 
     2041 CTCGCTACAG CAACAGTGAG GTGGTGACGG GCTCAGGGCT GGACAGCCAG AAGTCAGACG 
     2101 AGGAGTATCG CGAGCTCTTC GACCTAGCCC TGCGGGGTCT GCAGCTTCTA TCCAAGTGGA 
     2161 GCGCCCACGT CATGGAGGTG TACTCTTGGA AGCTGGTTCA TCCCACAGAC AAGTTCTGCA 
     2221 ACAAGGACTG TCCTGGCACC GCGGAGGAAT ATGAGAGAGC CACACGCTAC AATTACACCA 
     2281 GTGAGGAAAA ATTTGCCTTC GTTGAGGTGA TCGCCATGAT CAAAGGCCTG CAGGTGCTCA 
     2341 TGGGCAGGAT GGAGAGCGTC TTCAACCAGG CCATCAGGAA CACCATCTAC GCGGCATTGC 
     2401 AGGACTTCGC CCAGGTGACG CTGCGTGAGC CCCTGCGGCA GGCGGTACGG AAGAAGAAGA 
     2461 ATGTCCTCAT CAGCGTCCTA CAGGCAATTC GAAAGACCAT CTGTGACTGG GAGGGAGGGC 
     2521 GAGAGCCCCC TAATGACCCA TGCTTGAGAG GGGAGAAGGA CCCCAAAGGT GGATTTGATA 
     2581 TCAAGGTGCC CCGGCGTGCT GTGGGGCCAT CCAGCACACA GCTGTACATG GTGCGGACCA 
     2641 TGCTTGAATC ACTCATTGCA GACAAAAGCG GCTCCAAGAA GACCCTGAGG AGCAGCCTGG 
     2701 ATGGACCCAT TGTCCTCGCC ATAGAGGACT TTCACAAACA GTCCTTCTTC TTCACACATC 
     2761 TGCTCAACAT CAGTGAAGCC CTGCAGCAGT GTTGTGACCT CTCCCAGCTC TGGTTCCGAG 
     2821 AATTCTTCCT GGAGTTAACC ATGGGCCGAC GAATCCAGTT CCCCATCGAG ATGTCCATGC 
     2881 CCTGGATTCT AACGGACCAT ATCCTGGAAA CCAAAGAACC TTCCATGATG GAGTATGTCC 
     2941 TCTACCCTCT GGATCTGTAC AACGACAGCG CCTACTATGC TCTGACCAAG TTTAAAAAGC 
     3001 AGTTCCTGTA CGATGAGATA GAAGCTGAGG TGAACCTGTG TTTTGATCAG TTTGTCTACA 
     3061 AGCTGGCAGA CCAGATCTTT GCTTACTACA AAGCCATGGC TGGCAGTGTC CTGTTGGATA 
     3121 AACGTTTTCG AGCTGAGTGT AAGAATTATG GCGTCATCAT TCCGTATCCA CCGTCCAATC 
     3181 GCTATGAAAC ACTGCTGAAG CAGAGACACG TCCAGCTGTT GGGTAGATCA ATTGACTTGA 
     3241 ACAGACTCAT TACCCAGCGC ATCTCTGCCG CCATGTATAA ATCCTTGGAC CAAGCTATCA 
     3301 GCCGCTTTGA GAGTGAGGAC CTGACCTCCA TTGTGGAGCT GGAGTGGCTG CTGGAGATTA 
     3361 ACCGGCTCAC GCATCGGCTG CTCTGTAAGC ATATGACGCT GGACAGCTTC GATGCCATGT 
     3421 TCCGAGAGGC CAATCACAAT GTGTCCGCCC CCTATGGCCG TATCACCCTG CATGTCTTCT 
     3481 GGGAACTGAA CTTTGACTTT CTCCCCAACT ACTGCTACAA TGGGTCCACT AACCGTTTTG 
     3541 TGCGGACTGC CATTCCTTTC ACCCAAGAAC CACAACGAGA CAAACCTGCC AACGTCCAGC 
     3601 CTTATTACCT CTATGGATCC AAGCCTCTCA ACATTGCCTA CAGCCACATC TACAGCTCCT 
     3661 ACAGGAATTT CGTGGGGCCA CCTCATTTCA AGACTATCTG CAGACTCCTG GGTTATCAGG 
     3721 GCATCGCTGT GGTCATGGAG GAACTGCTAA AGATTGTGAA GAGCTTGCTC CAAGGAACCA 
     3781 TTCTCCAGTA TGTGAAAACA CTGATAGAGG TGATGCCCAA GATATGCCGC TTGCCCCGAC 
     3841 ATGAGTATGG CTCCCCAGGG ATCCTGGAGT TCTTCCACCA CCAGCTGAAG GACATCATTG 
     3901 AGTACGCAGA GCTCAAAACA GACGTGTTCC AGAGCCTGAG GGAAGTGGGC AATGCCATCC 
     3961 TCTTCTGCCT CCTCATAGAG CAAGCTCTGT CTCAGGAGGA GGTCTGCGAT TTGCTCCATG 
     4021 CCGCACCCTT CCAAAACATC TTGCCTAGAG TCTACATCAA AGAGGGGGAG CGCCTGGAGG 
     4081 TCCGGATGAA ACGTCTGGAA GCCAAGTATG CCCCGCTCCA CCTGGTCCCT CTGATCGAGC 
     4141 GGCTGGGGAC CCCTCAGCAA ATCGCCATTG CTCGCGAGGG TGACCTCCTG ACCAAGGAGC 
     4201 GGCTGTGCTG TGGCCTGTCC ATGTTCGAGG TCATCCTGAC CCGCATTCGG AGCTACCTGC 
     4261 AGGACCCCAT CTGGCGGGGC CCACCGCCCA CCAATGGCGT CATGCACGTC GATGAGTGTG 
     4321 TGGAGTTCCA CCGGCTGTGG AGCGCCATGC AGTTCGTGTA CTGCATCCCT GTGGGAACCA 
     4381 ACGAGTTCAC AGCTGAGCAG TGTTTCGGCG ATGGCTTGAA CTGGGCTGGT TGCTCCATCA 
     4441 TTGTCCTGCT GGGCCAGCAG CGTCGCTTTG ACCTGTTCGA CTTCTGTTAC CACCTGCTAA 
     4501 AAGTGCAGAG GCAGGACGGG AAGGATGAAA TCATTAAGAA TGTGCCCCTG AAGAAGATGG 
     4561 CCGACCGGAT CAGGAAGTAT CAGATCTTGA ACAATGAGGT TTTTGCCATC CTGAACAAAT 
     4621 ACATGAAGTC CGTGGAGACA GACAGTTCCA CTGTGGAGCA TGTGCGCTGC TTCCAGCCAC 
     4681 CCATCCACCA GTCCTTGGCC ACCACTTGCA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4741 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4801 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4861 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4921 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4981 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     5041 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5101 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5161 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5221 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5281 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5341 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5401 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5461 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5521 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5581 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5641 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5701 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5761 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5821 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5881 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5941 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     6001 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6061 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6121 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6181 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6241 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6301 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6361 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6421 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6481 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6541 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6601 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6661 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6721 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6781 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6841 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6901 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6961 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     7021 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7081 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7141 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7201 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7261 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7321 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7381 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7441 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7501 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7561 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7621 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7681 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7741 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7801 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7861 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7921 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7981 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     8041 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8101 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8161 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8221 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8281 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8341 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8401 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8461 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8521 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//