LOCUS       RC240057                8559 bp    DNA     circular  SYN 
DEFINITION  STAG3 (myc-DDK-tagged) - Human stromal antigen 3 (STAG3), transcript variant 3
ACCESSION   NM_001282717
VERSION     NM_001282717.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4716..4807 
                     /label="Myc-DDK"  
     misc_feature    4837..5422 
                     /label="PolyA signal"  
     misc_feature    4894..4913 
                     /label="Restriction Enzyme"  
     misc_feature    5698..6370 
                     /label="ColE1"  
     misc_feature    6800..7594 
                     /label="Kan/Neo"  
     misc_feature    7629..7931 
                     /label="SV40 ori"  
     misc_feature    8017..8494 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4698..4704 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4698 
                     /label="STAG3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTTCCCCG TTGCAAAGAG CTGTGGGAGA TACCAAGAGG GCCTTGTCTG 
     1081 CATCTTCTAG TTCCTCTGCC AGTCTACCCT TTGATGACAG GGACTCAAAC CATACCTCAG 
     1141 AGGGGAATGG CGACTCTTTG TTAGCTGATG AAGACACTGA CTTTGAAGAC AGCTTGAATC 
     1201 GCAATGTGAA GAAGAGAGCA GCAAAACGAC CACCGAAAAC AACACCGGTG GCAAAACATC 
     1261 CAAAGAAAGG GTCCCGAGTG GTACATCGTC ATAGCCGGAA ACAGTCAGAG CCACCAGCCA 
     1321 ATGATCTTTT CAATGCTGTG AAAGCCGCCA AAAGTGACAT GCAGTCTTTG GTAGATGAGT 
     1381 GGCTGGATAG CTACAAGCAA GACCAGGATG CAGGATTTCT GGAGCTTGTT AACTTTTTCA 
     1441 TCCAATCTTG CGGATGTAAA GGCATTGTGA CCCCTGAGAT GTTCAAGAAG ATGTCCAACT 
     1501 CAGAGATCAT CCAGCACCTA ACAGAGCAGT TTAATGAGGA CTCGGGGGAC TACCCTCTCA 
     1561 TAGCTCCAGG TCCATCCTGG AAGAAGTTCC AGGGCAGCTT CTGTGAATTT GTGAGGACAT 
     1621 TGGTCTGTCA GTGCCAGTAC AGCCTCCTCT ATGATGGCTT CCCTATGGAC GACCTCATCT 
     1681 CCCTGCTCAC TGGCCTCTCA GACTCACAAG TCCGCGCCTT CCGTCACACT AGCACCCTGG 
     1741 CTGCTATGAA ACTGATGACC TCCCTGGTAA AAGTTGCCCT CCAACTGAGT GTGCACCAAG 
     1801 ATAACAATCA GCGTCAGTAT GAGGCTGAAA GAAACAAGGG GCCAGGGCAG AGGGCACCTG 
     1861 AGCGGCTGGA GAGCCTGTTG GAGAAACGCA AAGAGCTCCA AGAGCATCAA GAGGAGATTG 
     1921 AGGGGATGAT GAATGCCCTC TTCAGGGGTG TCTTTGTTCA TCGGTACAGG GATGTCCTTC 
     1981 CTGAGATCCG TGCTATCTGC ATTGAGGAAA TTGGGTGTTG GATGCAAAGC TACAGCACGT 
     2041 CTTTCCTCAC CGACAGCTAT TTAAAATATA TTGGTTGGAC TCTGCATGAT AAGCACCGAG 
     2101 AAGTCCGCCT GAAGTGTGTG AAGGCCCTGA AAGGGCTGTA CGGTAACCGG GACCTGACCA 
     2161 CACGCCTGGA GCTCTTCACC AGCCGCTTCA AGGACCGGAT GGTTTCCATG GTCATGGACA 
     2221 GAGAGTATGA TGTGGCAGTG GAGGCTGTCA GATTACTGAT ACTTATCCTT AAGAACATGG 
     2281 AAGGGGTGCT GACGGACGCG GATTGTGAGA GCGTCTACCC AGTTGTGTAT GCCTCTCATC 
     2341 GAGGCCTGGC CTCTGCCGCA GGCGAATTTC TGTACTGGAA ACTCTTCTAC CCTGAGTGCG 
     2401 AGATAAGAAT GATGGGTGGA AGAGAGCAAC GCCAGAGCCC AGGCGCCCAG AGGACTTTCT 
     2461 TCCAGCTTCT GCTGTCCTTC TTTGTGGAGA GCGAGCTCCA TGACCACGCT GCTTACTTAG 
     2521 TAGACAGTCT GTGGGACTGT GCAGGGGCTC GGCTGAAGGA CTGGGAGGGT CTGACAAGCC 
     2581 TGCTGCTGGA GAAGGACCAG AACCTGGGTG ATGTGCAGGA GAGCACACTG ATAGAAATCC 
     2641 TTGTGTCCAG TGCCCGGCAA GCTTCAGAGG GGCACCCGCC TGTGGGCCGG GTCACTGGGA 
     2701 GGAAGGGCTT AACCTCTAAG GAGCGCAAGA CCCAAGCCGA TGACAGGGTG AAGTTGACTG 
     2761 AGCACCTCAT CCCCCTGCTG CCCCAGCTCC TGGCCAAGTT CTCAGCTGAT GCAGAGAAGG 
     2821 TCACTCCCCT GCTCCAGCTT CTCAGCTGCT TTGACCTCCA CATCTACTGC ACTGGGCGCT 
     2881 TGGAGAAGCA CCTGGAGCTG TTCCTGCAGC AACTCCAGGA GGTGGTGGTG AAGCATGCAG 
     2941 AGCCAGCGGT GCTTGAGGCT GGGGCGCATG CCCTCTACCT GCTCTGTAAT CCCGAATTCA 
     3001 CTTTCTTCAG CCGGGCGGAC TTTGCCCGCA GCCAGCTAGT AGATTTGCTG ACTGACCGCT 
     3061 TCCAGCAGGA GCTTGAAGAG CTGTTACAGT CGTCCTTCCT AGATGAGGAT GAGGTATATA 
     3121 ATCTGGCAGC CACTCTGAAA CGCCTCTCTG CCTTCTACAA CACTCATGAC CTGACTCGCT 
     3181 GGGAGCTCTA TGAGCCATGT TGCCAACTCC TGCAGAAGGC TGTGGACACA GGAGAGGTTC 
     3241 CTCACCAGGT TATCCTGCCA GCCTTGACTC TTGTCTATTT TTCCATTCTC TGGACACTAA 
     3301 CCCACATTTC TAAATCAGAT GCTTCCCAGA AGCAGCTGTC GAGTTTGAGG GACAGAATGG 
     3361 TGGCCTTCTG TGAACTCTGC CAGAGTTGCC TCTCAGATGT GGATACTGAG ATCCAGGAGC 
     3421 AGGCTTTTGT CTTATTAAGT GATCTACTTC TCATCTTTAG CCCTCAGATG ATTGTTGGGG 
     3481 GCCGTGATTT CCTTAGGCCA CTTGTCTTTT TTCCTGAAGC TACTCTCCAG TCTGAGCTAG 
     3541 CCAGCTTCCT CATGGACCAC GTCTTCATCC AGCCGGGAGA CCTGGGCAGT GGTGATTCCC 
     3601 AGGAGGATCA TTTACAGATA GAGCGGCTAC ACCAGCGGCG CCGCCTCCTA GCCGGGTTCT 
     3661 GCAAGCTGTT GCTTTATGGG GTGCTGGAGA TGGATGCAGC CTCAGATGTT TTCAAACACT 
     3721 ACAACAAGTT CTACAATGAC TATGGTGACA TTATCAAGGA AACATTAACT AGAGCAAGGC 
     3781 AGATTGACCG AAGTCATTGT TCCCGAATCC TGCTGCTGAG CCTCAAGCAG CTGTACACAG 
     3841 AACTGCTGCA GGAGCATGGG CCCCAGGGCC TGAATGAGCT TCCTGCCTTC ATCGAGATGA 
     3901 GGGACCTGGC CCGGAGGTTT GCCTTGAGTT TTGGACCCCA GCAGCTGCAG AACCGTGACC 
     3961 TCGTGGTCAT GCTACACAAG GAAGGCATCC AGTTCTCCTT GTCTGAGCTT CCTCCAGCTG 
     4021 GCTCCTCCAA TCAGCCTCCA AATCTGGCAT TCCTGGAGCT CCTTTCAGAG TTTTCCCCCC 
     4081 GACTCTTCCA TCAGGACAAG CAGCTTTTAC TGTCCTATCT AGAAAAGTGC CTGCAGCATG 
     4141 TCTCCCAGGC ACCTGGCCAT CCCTGGGGCC CAGTCACCAC CTACTGCCAC TCCCTCAGCC 
     4201 CTGTGGAGAA CACAGCAGAG ACCAGCCCTC AGGTCCTCCC CAGCTCCAAG AGGAGGCGCG 
     4261 TTGAAGGGCC TGCCAAGCCT AACAGAGAGG ACGTCTCCTC GTCCCAGGAA GAAAGTCTGC 
     4321 AGCTGAACAG CATCCCGCCC ACGCCCACCC TCACCTCCAC AGCTGTGAAG AGCAGGCAGC 
     4381 CCCTGTGGGG GTTGAAAGAG ATGGAGGAAG AAGATGGCTC AGAGTTGGAT TTTGCCCAGG 
     4441 GCAGTCAGCC CGTCGCAGGC ACCGAGAGGT CAAGGTTCTT GGGTCCACAA TATTTCCAGA 
     4501 CTCCACACAA CCCTTCAGGT CCTGGCCTGG GCAACCAGCT GATGCGACTC AGCCTTATGG 
     4561 AAGAGGACGA GGAAGAAGAG TTAGAAATCC AGGATGAGTC AAATGAAGAA CGGCAGGATA 
     4621 CAGACATGCA AGCAAGTAGC TACTCTTCCA CCAGTGAGCG CGGGCTGGAC CTCTTAGATT 
     4681 CTACAGAGCT GGATATTGAG GATTTCACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4741 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4801 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4861 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4921 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4981 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     5041 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     5101 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5161 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5221 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5281 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5341 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5401 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5461 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5521 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5581 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5641 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5701 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5761 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5821 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5881 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5941 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     6001 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     6061 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6121 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6181 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6241 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6301 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6361 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6421 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6481 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6541 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6601 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6661 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6721 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6781 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6841 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6901 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6961 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     7021 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     7081 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7141 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7201 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7261 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7321 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7381 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7441 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7501 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7561 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7621 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7681 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7741 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7801 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7861 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7921 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7981 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     8041 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     8101 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     8161 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8221 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8281 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8341 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8401 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8461 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8521 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//