LOCUS       RC240056                8577 bp    DNA     circular  SYN 
DEFINITION  PEX1 (myc-DDK-tagged) - Human peroxisomal biogenesis factor 1 (PEX1), transcript variant 2
ACCESSION   NM_001282677
VERSION     NM_001282677.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4732..4823 
                     /label="Myc-DDK"  
     misc_feature    4853..5438 
                     /label="PolyA signal"  
     misc_feature    4910..4929 
                     /label="Restriction Enzyme"  
     misc_feature    5714..6386 
                     /label="ColE1"  
     misc_feature    6816..7610 
                     /label="Kan/Neo"  
     misc_feature    7645..7947 
                     /label="SV40 ori"  
     misc_feature    8033..8510 
                     /label="f1 ori"  
     misc_feature    1029..1037 
                     /label="Restriction Enzyme"  
     misc_feature    4707..4714 
                     /label="Restriction Enzyme"  
     misc_feature    1029..4707 
                     /label="PEX1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCGG CGCGCCCATG TGGGGCAGCG ATCGCCTGGC GGGTGCTGGG GGAGGCGGGG 
     1081 CGGCAGTGAC TGTGGCCTTC ACCAACGCTC GCGACTGCTT CCTCCACCTG CCGCGGCGTC 
     1141 TCGTGGCCCA GCTGCATCTG CTGCAGAATC AAGCTATAGA AGTGGTCTGG AGTCACCAGC 
     1201 CTGCATTCTT GAGCTGGGTG GAAGGCAGGC ATTTTAGTGA TCAAGGTGAA AATGTGGCTG 
     1261 AAATTAACAG ACAAGTTGGT CAAAAACTTG GACTCTCAAA TGGGGGACAG GTATTTCTCA 
     1321 AGCCATGTTC CCATGTGGTA TCTTGTCAAC AAGTTGAGGT GGAACCCCTC TCAGCAGATG 
     1381 ATTGGGAGAT ACTGGAGCTG CATGCTGTTT CCCTTGAACA ACATCTTCTA GATCAAATTC 
     1441 GAATAGTTTT TCCAAAAGCC ATTTTTCCTG TTTGGGTTGA TCAACAAACG TACATATTTA 
     1501 TCCAAATTGT TGCACTAATA CCAGCTGCCT CTTATGGAAG GCTGGAAACT GACACCAAAC 
     1561 TCCTTATTCA GCCAAAGACA CGCCGAGCCA AAGAGAATAC ATTTTCAAAA GCTGATGCTG 
     1621 AATATAAAAA ACTTCATAGT TATGGAAGAG ACCAGAAAGG AATGATGAAA GAACTTCAAA 
     1681 CCAAGCAACT TCAGTCAAAT ACTGTGGGAA TCACTGAATC TAATGAAAAC GAGTCAGAGA 
     1741 TTCCAGTTGA CTCATCATCA GTAGCAAGTT TATGGACTAT GATAGGAAGC ATTTTTTCCT 
     1801 TTCAATCTGA GAAGAAACAA GAGACATCTT GGGGTTTAAC TGAAATCAAT GCATTCAAAA 
     1861 ATATGCAGTC AAAGGTTGTT CCTCTAGACA ATATTTTCAG AGTATGCAAA TCTCAACCTC 
     1921 CTAGTATATA TAACGCGTCA GCAACCTCTG TTTTTCATAA ACACTGTGCC ATTCATGTAT 
     1981 TTCCATGGGA CCAGGAATAT TTTGATGTAG AGCCCAGCTT TACTGTGACA TATGGAAAGC 
     2041 TAGTTAAGCT ACTTTCTCCA AAGCAACAGC AAAGTAAAAC AAAACAAAAT GTGTTATCAC 
     2101 CTGAAAAAGA GAAGCAGATG TCAGAGCCAC TAGATCAAAA AAAAATTAGG TCAGATCATA 
     2161 ATGAAGAAGA TGAGAAGGCC TGTGTGCTAC AAGTAGTCTG GAATGGACTT GAAGAATTGA 
     2221 ACAATGCCAT CAAATATACC AAAAATGTAG AAGTTCTCCA TCTTGGGAAA GTCTGGATTC 
     2281 CAGATGACCT GAGGAAGAGA CTAAATATAG AAATGCATGC CGTAGTCAGG ATAACTCCAG 
     2341 TGGAAGTTAC CCCTAAAATT CCAAGATCTC TAAAGTTACA ACCTAGAGAG AATTTACCTA 
     2401 AAGACATAAG TGAAGAAGAC ATAAAAACTG TATTTTATTC ATGGCTACAG CAGTCTACTA 
     2461 CCACCATGCT TCCTTTGGTA ATATCAGAGG AAGAATTTAT TAAGCTGGAA ACTAAAGATG 
     2521 GACTGAAGGA ATTTTCTCTG AGTATAGTTC ATTCTTGGGA AAAAGAAAAA GATAAAAATA 
     2581 TTTTTCTGTT GAGTCCCAAT TTGCTGCAGA AGACTACAAT ACAAGTCCTT CTAGATCCTA 
     2641 TGGTAAAAGA AGAAAACAGT GAGGAAATTG ACTTTATTCT TCCTTTTTTA AAGCTGAGCT 
     2701 CTTTGGGAGG AGTGAATTCC TTAGGCGTAT CCTCCTTGGA GCACATCACT CACAGCCTCC 
     2761 TGGGACGCCC TTTGTCTCGG CAGCTGATGT CTCTTGTTGC AGGACTTAGG AATGGAGCTC 
     2821 TTTTACTCAC AGGAGGAAAG GGAAGTGGAA AATCAACTTT AGCCAAAGCA ATCTGTAAAG 
     2881 AAGCATTTGA CAAACTGGAT GCCCATGTGG AGAGAGTTGA CTGTAAAGCT TTACGAGCTT 
     2941 TGAATGATAT GATAAAAGAG TTTATCTCCA TGGGAAGTTT GGTTGCACTG ATTGCCACAA 
     3001 GTCAGTCTCA GCAATCTCTA CATCCTTTAC TTGTTTCTGC TCAAGGAGTT CACATATTTC 
     3061 AGTGCGTCCA ACACATTCAG CCTCCTAATC AGGAACAAAG ATGTGAAATT CTGTGTAATG 
     3121 TAATAAAAAA TAAATTGGAC TGTGATATAA ACAAGTTCAC CGATCTTGAC CTGCAGCATG 
     3181 TAGCTAAAGA AACTGGCGGG TTTGTGGCTA GAGATTTTAC AGTACTTGTG GATCGAGCCA 
     3241 TACATTCTCG ACTCTCTCGT CAGAGTATAT CCACCAGAGA AAAATTAGTT TTAACAACAT 
     3301 TGGACTTCCA AAAGGCTCTC CGCGGATTTC TTCCTGCGTC TTTGCGAAGT GTCAACCTGC 
     3361 ATAAACCTAG AGACCTGGGT TGGGACAAGA TTGGTGGGTT ACATGAAGTT AGGCAGATAC 
     3421 TCATGGATAC TATCCAGTTA CCTGCCAAGT ATCCAGAATT ATTTGCAAAC TTGCCCATAC 
     3481 GACAAAGAAC AGGAATACTG TTGTATGGTC CGCCTGGAAC AGGAAAAACC TTACTAGCTG 
     3541 GGGTAATTGC ACGAGAGAGT AGAATGAATT TTATAAGTGT CAAGGGGCCA GAGTTACTCA 
     3601 GCAAATACAT TGGAGCAAGT GAACAAGCTG TTCGGGATAT TTTTATTAGA GCACAGGCTG 
     3661 CAAAGCCCTG CATTCTTTTC TTTGATGAAT TTGAATCCAT TGCTCCTCGG CGGGGTCATG 
     3721 ATAATACAGG AGTTACAGAC CGAGTAGTTA ACCAGTTGCT GACTCAGTTG GATGGAGTAG 
     3781 AAGGCTTACA GGGTGTTTAT GTATTGGCTG CTACTAGTCG CCCTGACTTG ATTGACCCTG 
     3841 CCCTGCTTAG GCCTGGTCGA CTAGATAAAT GTGTATACTG TCCTCCTCCT GATCAGGTGT 
     3901 CACGTCTTGA AATTTTAAAT GTCCTCAGTG ACTCTCTACC TCTGGCAGAT GATGTTGACC 
     3961 TTCAGCATGT AGCATCAGTA ACTGACTCCT TTACTGGAGC TGATCTGAAA GCTTTACTTT 
     4021 ACAATGCCCA ATTGGAGGCC TTACATGGAA TGCTGCTCTC GAGTGGACTC CAGGATGGAA 
     4081 GTTCCAGCTC TGATAGTGAC CTAAGTCTGT CTTCAATGGT CTTTCTTAAC CATAGCAGTG 
     4141 GCTCTGACGA TTCAGCTGGA GATGGAGAAT GTGGCTTAGA TCAGTCCCTT GTTTCTTTAG 
     4201 AGATGTCCGA GATCCTTCCA GATGAATCAA AATTCAATAT GTACCGGCTC TACTTTGGAA 
     4261 GCTCTTATGA ATCAGAACTT GGAAATGGAA CCTCTTCTGA TTTGAGCTCA CAATGTCTCT 
     4321 CTGCACCAAG CTCCATGACT CAGGATTTGC CTGGAGTTCC TGGGAAAGAC CAGTTGTTTT 
     4381 CACAGCCTCC AGTGTTAAGG ACAGCTTCAC AAGAGGGTTG CCAAGAACTT ACACAAGAAC 
     4441 AAAGAGATCA ACTGAGGGCA GATATCAGTA TTATCAAAGG CAGATACCGG AGCCAAAGTG 
     4501 GAGAGGACGA ATCCATGAAC CAACCAGGAC CAATCAAAAC CAGACTGGCT ATTAGTCAGT 
     4561 CACATTTAAT GACTGCACTT GGTCACACAA GACCATCCAT TAGTGAAGAT GACTGGAAGA 
     4621 ATTTTGCTGA GCTATATGAA AGCTTTCAAA ATCCAAAGAG GAGAAAAAAT CAAAGTGGAA 
     4681 CAATGTTTCG ACCTGGACAG AAAGTAACTT TAGCAAGCGG ACCGACGCGT ACGCGGCCGC 
     4741 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4801 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4861 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4921 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4981 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5041 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5101 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5161 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5221 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5281 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5341 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5401 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5461 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5521 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5581 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5641 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5701 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5761 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5821 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5881 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5941 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6001 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6061 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6121 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6181 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6241 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6301 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6361 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6421 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6481 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6541 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6601 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6661 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6721 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6781 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6841 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6901 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6961 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7021 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7081 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7141 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7201 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7261 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7321 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7381 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7441 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7501 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7561 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7621 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7681 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7741 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7801 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7861 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7921 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7981 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8041 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8101 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8161 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8221 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8281 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8341 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8401 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8461 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8521 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//