LOCUS       RC240055                8556 bp    DNA     circular  SYN 
DEFINITION  STAG3 (myc-DDK-tagged) - Human stromal antigen 3 (STAG3), transcript variant 2
ACCESSION   NM_001282716
VERSION     NM_001282716.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4713..4804 
                     /label="Myc-DDK"  
     misc_feature    4834..5419 
                     /label="PolyA signal"  
     misc_feature    4891..4910 
                     /label="Restriction Enzyme"  
     misc_feature    5695..6367 
                     /label="ColE1"  
     misc_feature    6797..7591 
                     /label="Kan/Neo"  
     misc_feature    7626..7928 
                     /label="SV40 ori"  
     misc_feature    8014..8491 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4695..4701 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4695 
                     /label="STAG3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTTCCCCG TTGCAAAGAG CTGTGGGAGA TACCAAGAGG GCCTTGTCTG 
     1081 CATCTTCTAG TTCCTCTGCC AGTCTACCCT TTGATGACAG GGACTCAAAC CATACCTCAG 
     1141 AGGGGAATGG CGACTCTTTG TTAGCTGATG AAGACACTGA CTTTGAAGAC AGCTTGAATC 
     1201 GCAATGTGAA GAAGAGAGCA GCAAAACGAC CACCGAAAAC AACACCGGTG GCAAAACATC 
     1261 CAAAGAAAGG GTCCCGAGTG GTACATCGTC ATAGCCGGAA ACAGTCAGAG CCACCAGCCA 
     1321 ATGATCTTTT CAATGCTGTG AAAGCCGCCA AAAGTGACAT GCAGTCTTTG GTAGATGAGT 
     1381 GGCTGGATAG CTACAAGCAA GACCAGGATG CAGGATTTCT GGAGCTTGTT AACTTTTTCA 
     1441 TCCAATCTTG CGGATGTAAA GGCATTGTGA CCCCTGAGAT GTTCAAGAAG ATGTCCAACT 
     1501 CAGAGATCAT CCAGCACCTA ACAGAGCAGT TTAATGAGGA CTCGGGGGAC TACCCTCTCA 
     1561 TAGCTCCAGG TCCATCCTGG AAGAAGTTCC AGGGCAGCTT CTGTGAATTT GTGAGGACAT 
     1621 TGGTCTGTCA GTGCCAGTAC AGCCTCCTCT ATGATGGCTT CCCTATGGAC GACCTCATCT 
     1681 CCCTGCTCAC TGGCCTCTCA GACTCACAAG TCCGCGCCTT CCGTCACACT AGCACCCTGG 
     1741 CTGCTATGAA ACTGATGACC TCCCTGGTAA AAGTTGCCCT CCAACTGAGT GTGCACCAAG 
     1801 ATAACAATCA GCGTCAGTAT GAGGCTGAAA GAAACAAGGG GCCAGGGCAG AGGGCACCTG 
     1861 AGCGGCTGGA GAGCCTGTTG GAGAAACGCA AAGAGCTCCA AGAGCATCAA GAGGAGATTG 
     1921 AGGGGATGAT GAATGCCCTC TTCAGGGGTG TCTTTGTTCA TCGGTACAGG GATGTCCTTC 
     1981 CTGAGATCCG TGCTATCTGC ATTGAGGAAA TTGGGTGTTG GATGCAAAGC TACAGCACGT 
     2041 CTTTCCTCAC CGACAGCTAT TTAAAATATA TTGGTTGGAC TCTGCATGAT AAGCACCGAG 
     2101 AAGTCCGCCT GAAGTGTGTG AAGGCCCTGA AAGGGCTGTA CGGTAACCGG GACCTGACCA 
     2161 CACGCCTGGA GCTCTTCACC AGCCGCTTCA AGGACCGGAT GGTTTCCATG GTCATGGACA 
     2221 GAGAGTATGA TGTGGCAGTG GAGGCTGTCA GATTACTGAT ACTTATCCTT AAGAACATGG 
     2281 AAGGGGTGCT GACGGACGCG GATTGTGAGA GCGTCTACCC AGTTGTGTAT GCCTCTCATC 
     2341 GAGGCCTGGC CTCTGCCGCA GGCGAATTTC TGTACTGGAA ACTCTTCTAC CCTGAGTGCG 
     2401 AGATAAGAAT GATGGGTGGA AGAGAGCAAC GCCAGAGCCC AGGCGCCCAG AGGACTTTCT 
     2461 TCCAGCTTCT GCTGTCCTTC TTTGTGGAGA GCGAGCTCCA TGACCACGCT GCTTACTTAG 
     2521 TAGACAGTCT GTGGGACTGT GCAGGGGCTC GGCTGAAGGA CTGGGAGGGT CTGACAAGCC 
     2581 TGCTGCTGGA GAAGGACCAG AACCTGGGTG ATGTGCAGGA GAGCACACTG ATAGAAATCC 
     2641 TTGTGTCCAG TGCCCGGCAA GCTTCAGAGG GGCACCCGCC TGTGGGCCGG GTCACTGGGA 
     2701 GGAAGGGCTT AACCTCTAAG GAGCGCAAGA CCCAAGCCGA TGACAGGGTG AAGTTGACTG 
     2761 AGCACCTCAT CCCCCTGCTG CCCCAGCTCC TGGCCAAGTT CTCAGCTGAT GCAGAGAAGG 
     2821 TCACTCCCCT GCTCCAGCTT CTCAGCTGCT TTGACCTCCA CATCTACTGC ACTGGGCGCT 
     2881 TGGAGAAGCA CCTGGAGCTG TTCCTGCAGC AACTCCAGGA GGTGGTGGTG AAGCATGCAG 
     2941 AGCCAGCGGT GCTTGAGGCT GGGGCGCATG CCCTCTACCT GCTCTGTAAT CCCGAATTCA 
     3001 CTTTCTTCAG CCGGGCGGAC TTTGCCCGCA GCCAGCTAGT AGATTTGCTG ACTGACCGCT 
     3061 TCCAGCAGGA GCTTGAAGAG CTGTTACAGT CGTCCTTCCT AGATGAGGAT GAGGTATATA 
     3121 ATCTGGCAGC CACTCTGAAA CGCCTCTCTG CCTTCTACAA CACTCATGAC CTGACTCGCT 
     3181 GGGAGCTCTA TGAGCCATGT TGCCAACTCC TGCAGAAGGC TGTGGACACA GGAGAGGTTC 
     3241 CTCACCAGGT TATCCTGCCA GCCTTGACTC TTGTCTATTT TTCCATTCTC TGGACACTAA 
     3301 CCCACATTTC TAAATCAGAT GCTTCCCAGA AGCAGCTGTC GAGTTTGAGG GACAGAATGG 
     3361 TGGCCTTCTG TGAACTCTGC CAGAGTTGCC TCTCAGATGT GGATACTGAG ATCCAGGAGC 
     3421 AGGCTTTTGT CTTATTAAGT GATCTACTTC TCATCTTTAG CCCTCAGATG ATTGTTGGGG 
     3481 GCCGTGATTT CCTTAGGCCA CTTGTCTTTT TTCCTGAAGC TACTCTCCAG TCTGAGCTAG 
     3541 CCAGCTTCCT CATGGACCAC GTCTTCATCC AGCCGGGAGA CCTGGGCAGT GGTGATTCCC 
     3601 AGGAGGATCA TTTACAGATA GAGCGGCTAC ACCAGCGGCG CCGCCTCCTA GCCGGGTTCT 
     3661 GCAAGCTGTT GCTTTATGGG GTGCTGGAGA TGGATGCAGC CTCAGATGTT TTCAAACACT 
     3721 ACAACAAGTT CTACAATGAC TATGGTGACA TTATCAAGGA AACATTAACT AGAGCAAGGC 
     3781 AGATTGACCG AAGTCATTGT TCCCGAATCC TGCTGCTGAG CCTCAAGCAG CTGTACACAG 
     3841 AACTGCTGCA GGAGCATGGG CCCCAGGGCC TGAATGAGCT TCCTGCCTTC ATCGAGATGA 
     3901 GGGACCTGGC CCGGAGGTTT GCCTTGAGTT TTGGACCCCA GCAGCTGCAG AACCGTGACC 
     3961 TCGTGGTCAT GCTACACAAG GAAGGCATCC AGTTCTCCTT GTCTGAGCTT CCTCCAGCTG 
     4021 GCTCCTCCAA TCAGCCTCCA AATCTGGCAT TCCTGGAGCT CCTTTCAGAG TTTTCCCCCC 
     4081 GACTCTTCCA TCAGGACAAG CAGCTTTTAC TGTCCTATCT AGAAAAGTGC CTGCAGCATG 
     4141 TCTCCCAGGC ACCTGGCCAT CCCTGGGGCC CAGTCACCAC CTACTGCCAC TCCCTCAGCC 
     4201 CTGTGGAGAA CACAGCAGAG ACCAGCCCTC AGGTCCTCCC CAGCTCCAAG AGGAGGCGCG 
     4261 TTGAAGGGCC TGCCAAGCCT AACAGAGAGG ACGTCTCCTC GTCCCAGGAA GAAAGTCTGC 
     4321 AGCTGAACAG CATCCCGCCC ACGCCCACCC TCACCTCCAC AGCTGTGAAG AGCAGGCAGC 
     4381 CCCTGTGGGG GTTGAAAGAG ATGGAGGAAG AAGATGGCTC AGAGTTGGAT TTTGCCCAGG 
     4441 GTCAGCCCGT CGCAGGCACC GAGAGGTCAA GGTTCTTGGG TCCACAATAT TTCCAGACTC 
     4501 CACACAACCC TTCAGGTCCT GGCCTGGGCA ACCAGCTGAT GCGACTCAGC CTTATGGAAG 
     4561 AGGACGAGGA AGAAGAGTTA GAAATCCAGG ATGAGTCAAA TGAAGAACGG CAGGATACAG 
     4621 ACATGCAAGC AAGTAGCTAC TCTTCCACCA GTGAGCGCGG GCTGGACCTC TTAGATTCTA 
     4681 CAGAGCTGGA TATTGAGGAT TTCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4741 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4801 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4861 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4921 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4981 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5041 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5101 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5161 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5221 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5281 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5341 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5401 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5461 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5521 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5581 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5641 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5701 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5761 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5821 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5881 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5941 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6001 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6061 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6121 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6181 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6241 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6301 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6361 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6421 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6481 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6541 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6601 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6661 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6721 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6781 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6841 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6901 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6961 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7021 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7081 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7141 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7201 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7261 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7321 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7381 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7441 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7501 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7561 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7621 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7681 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7741 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7801 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7861 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7921 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7981 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8041 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8101 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8161 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8221 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8281 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8341 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8401 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8461 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8521 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//