LOCUS       RC240049                8532 bp    DNA     circular  SYN 
DEFINITION  MTR (myc-DDK-tagged) - Human 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant 2
ACCESSION   NM_001291939
VERSION     NM_001291939.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4687..4778 
                     /label="Myc-DDK"  
     misc_feature    4808..5393 
                     /label="PolyA signal"  
     misc_feature    4865..4884 
                     /label="Restriction Enzyme"  
     misc_feature    5669..6341 
                     /label="ColE1"  
     misc_feature    6771..7565 
                     /label="Kan/Neo"  
     misc_feature    7600..7902 
                     /label="SV40 ori"  
     misc_feature    7988..8465 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4662..4669 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4662 
                     /label="MTR"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCACCCGCG CTCCAAGACC TGTCGCAACC CGAAGGTCTG AAGAAAACCC 
     1081 TGCGGGATGA GATCAATGCC ATTCTGCAGA AGAGGATTAT GGTGCTGGAT GGAGGGATGG 
     1141 GGACCATGAT CCAGCGGGAG AAGCTAAACG AAGAACACTT CCGAGGTCAG GAATTTAAAG 
     1201 ATCATGCCAG GCCGCTGAAA GGCAACAATG ACATTTTAAG TATAACTCAG CCTGATGTCA 
     1261 TTTACCAAAT CCATAAGGAA TACTTGCTGG CTGGGGCAGA TATCATTGAA ACAAATACTT 
     1321 TTAGCAGCAC TAGTATTGCC CAAGCTGACT ATGGCCTTGA ACACTTGGCC TACCGGATGA 
     1381 ACATGTGCTC TGCAGGAGTG GCCAGAAAAG CTGCCGAGGA GGTAACTCTC CAGACAGGAA 
     1441 TTAAGAGGTT TGTGGCAGGG GCTCTGGGTC CGACTAATAA GACACTCTCT GTGTCCCCAT 
     1501 CTGTGGAAAG GCCGGATTAT AGGAACATCA CATTTGATGA GCTTGTTGAA GCATACCAAG 
     1561 AGCAGGCCAA AGGACTTCTG GATGGCGGGG TTGATATCTT ACTCATTGAA ACTATTTTTG 
     1621 ATACTGCCAA TGCCAAGGCA GCCTTGTTTG CACTCCAAAA TCTTTTTGAG GAGAAATATG 
     1681 CTCCCCGGCC TATCTTTATT TCAGGGACGA TCGTTGATAA AAGTGGGCGG ACTCTTTCCG 
     1741 GACAGACAGG AGAGGGATTT GTCATCAGCG TGTCTCATGG AGAACCACTC TGCATTGGAT 
     1801 TAAATTGTGC TTTGGGTGCA GCTGAAATGA GACCTTTTAT TGAAATAATT GGAAAATGTA 
     1861 CAACAGCCTA TGTCCTCTGT TATCCCAATG CAGGTCTTCC CAACACCTTT GGTGACTATG 
     1921 ATGAAACGCC TTCTATGATG GCCAAGCACC TAAAGGATTT TGCTATGGAT GGCTTGGTCA 
     1981 ATATAGTTGG AGGATGCTGT GGGTCAACAC CAGATCATAT CAGGGAAATT GCTGAAGCTG 
     2041 TGAAAAATTG TAAGCCTAGA GTTCCACCTG CCACTGCTTT TGAAGGACAT ATGTTACTGT 
     2101 CTGGTCTAGA GCCCTTCAGG ATTGGACCGT ACACCAACTT TGTTAACATT GGAGAGCGCT 
     2161 GTAATGTTGC AGGATCAAGG AAGTTTGCTA AACTCATCAT GGCAGGAAAC TATGAAGAAG 
     2221 CCTTGTGTGT TGCCAAAGTG CAGGTGGAAA TGGGAGCCCA GGTGTTGGAT GTCAACATGG 
     2281 ATGATGGCAT GCTAGATGGT CCAAGTGCAA TGACCAGATT TTGCAACTTA ATTGCTTCCG 
     2341 AGCCAGACAT CGCAAAGGTA CCTTTGTGCA TCGACTCCTC CAATTTTGCT GTGATTGAAG 
     2401 CTGGGTTAAA GTGCTGCCAA GGGAAGTGCA TTGTCAATAG CATTAGTCTG AAGGAAGGAG 
     2461 AGGACGACTT CTTGGAGAAG GCCAGGAAGA TTAAAAAGTA TGGAGCTGCT ATGGTGGTCA 
     2521 TGGCTTTTGA TGAAGAAGGA CAGGCAACAG AAACAGACAC AAAAATCAGA GTGTGCACCC 
     2581 GGGCCTACCA TCTGCTTGTG AAAAAACTGG GCTTTAATCC AAATGACATT ATTTTTGACC 
     2641 CTAATATCCT AACCATTGGG ACTGGAATGG AGGAACACAA CTTGTATGCC ATTAATTTTA 
     2701 TCCATGCAAC AAAAGTCATT AAAGAAACAT TACCTGGAGC CAGAATAAGT GGAGGTCTTT 
     2761 CCAACTTGTC CTTCTCCTTC CGAGGAATGG AAGCCATTCG AGAAGCAATG CATGGGGTTT 
     2821 TCCTTTACCA TGCAATCAAG TCTGGCATGG ACATGGGGAT AGTGAATGCT GGAAACCTCC 
     2881 CTGTGTATGA TGATATCCAT AAGGAACTTC TGCAGCTCTG TGAAGATCTC ATCTGGAATA 
     2941 AAGACCCTGA GGCCACTGAG AAGCTCTTAC GTTATGCCCA GACTCAAGGC ACAGGAGGGA 
     3001 AGAAAGTCAT TCAGACTGAT GAGTGGAGAA ATGGCCCTGT CGAAGAACGC CTTGAGTATG 
     3061 CCCTTGTGAA GGTTATAAAG TCAGCCCGGG TTATGAAGAA GGCTGTTGGC CACCTTATCC 
     3121 CTTTCATGGA AAAAGAAAGA GAAGAAACCA GAGTGCTTAA CGGCACAGTA GAAGAAGAGG 
     3181 ACCCTTACCA GGGCACCATC GTGCTGGCCA CTGTTAAAGG CGACGTGCAC GACATAGGCA 
     3241 AGAACATAGT TGGAGTAGTC CTTGGCTGCA ATAATTTCCG AGTTATTGAT TTAGGAGTCA 
     3301 TGACTCCATG TGATAAGATA CTGAAAGCTG CTCTTGACCA CAAAGCAGAT ATAATTGGCC 
     3361 TGTCAGGACT CATCACTCCT TCCCTGGATG AAATGATTTT TGTTGCCAAG GAAATGGAGA 
     3421 GATTAGCTAT AAGGATTCCA TTGTTGATTG GAGGAGCAAC CACTTCAAAA ACCCACACAG 
     3481 CAGTTAAAAT AGCTCCGAGA TACAGTGCAC CTGTAATCCA TGTCCTGGAC GCGTCCAAGA 
     3541 GTGTGGTGGT GTGTTCCCAG CTGTTAGATG AAAATCTAAA GGATGAATAC TTTGAGGAAA 
     3601 TCATGGAAGA ATATGAAGAT ATTAGACAGG ACCATTATGA GTCTCTCAAG GAGAGGAGAT 
     3661 ACTTACCCTT AAGTCAAGCC AGAAAAAGTG GTTTCCAAAT GGATTGGCTG TCTGAACCTC 
     3721 ACCCAGTGAA GCCCACGTTT ATTGGGACCC AGGTCTTTGA AGACTATGAC CTGCAGAAGC 
     3781 TGGTGGACTA CATTGACTGG AAGCCTTTCT TTGATGTCTG GCAGCTCCGG GGCAAGTACC 
     3841 CGAATCGAGG CTTTCCCAAG ATATTTAACG ACAAAACAGT AGGTGGAGAG GCCAGGAAGG 
     3901 TCTACGATGA TGCCCACAAT ATGCTGAACA CACTGATTAG TCAAAAGAAA CTCCGGGCCC 
     3961 GGGGTGTGGT TGGGTTCTGG CCAGCACAGA GTATCCAAGA CGACATTCAC CTGTACGCAG 
     4021 AGGCTGCTGT GCCCCAGGCT GCAGAGCCCA TAGCCACCTT CTATGGGTTA AGGCAACAGG 
     4081 CTGAGAAGGA CTCTGCCAGC ACGGAGCCAT ACTACTGCCT CTCAGACTTC ATCGCTCCCT 
     4141 TGCATTCTGG CATCCGTGAC TACCTGGGCC TGTTTGCCGT TGCCTGCTTT GGGGTAGAAG 
     4201 AGCTGAGCAA GGCCTATGAG GATGATGGTG ACGACTACAG CAGCATCATG GTCAAGGCGC 
     4261 TGGGGGACCG GCTGGCAGAG GCCTTTGCAG AAGAGCTCCA TGAAAGAGTT CGCCGAGAAC 
     4321 TGTGGGCCTA CTGTGGCAGT GAGCAGCTGG ACGTCGCAGA CCTGCGCAGG CTGCGGTACA 
     4381 AGGGCATCCG CCCGGCTCCT GGCTACCCCA GCCAGCCCGA CCACACCGAG AAGCTCACCA 
     4441 TGTGGAGACT CGCAGACATC GAGCAGTCTA CAGGCATTAG GTTAACAGAA TCATTAGCAA 
     4501 TGGCACCTGC TTCAGCAGTC TCAGGCCTCT ACTTCTCCAA TTTGAAGTCC AAATATTTTG 
     4561 CTGTGGGGAA GATTTCCAAG GATCAGGTTG AGGATTATGC ATTGAGGAAG AACATATCTG 
     4621 TGGCTGAGGT TGAGAAATGG CTTGGACCCA TTTTGGGATA TGATACAGAC AGCGGACCGA 
     4681 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4741 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4801 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4861 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4921 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4981 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     5041 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     5101 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5161 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5221 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5281 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5341 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5401 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5461 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5521 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5581 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5641 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5701 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5761 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5821 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5881 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5941 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     6001 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     6061 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6121 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6181 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6241 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6301 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6361 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6421 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6481 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6541 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6601 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6661 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6721 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6781 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6841 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6901 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6961 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     7021 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     7081 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7141 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7201 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7261 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7321 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7381 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7441 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7501 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7561 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7621 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7681 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7741 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7801 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7861 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7921 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7981 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     8041 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     8101 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     8161 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     8221 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8281 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8341 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8401 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8461 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8521 AACGCGAATT TT
//