LOCUS       RC240047                8529 bp    DNA     circular  SYN 
DEFINITION  GAK (myc-DDK-tagged) - Human cyclin G associated kinase (GAK), transcript variant 2
ACCESSION   NM_001286833
VERSION     NM_001286833.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4684..4775 
                     /label="Myc-DDK"  
     misc_feature    4805..5390 
                     /label="PolyA signal"  
     misc_feature    4862..4881 
                     /label="Restriction Enzyme"  
     misc_feature    5666..6338 
                     /label="ColE1"  
     misc_feature    6768..7562 
                     /label="Kan/Neo"  
     misc_feature    7597..7899 
                     /label="SV40 ori"  
     misc_feature    7985..8462 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4659..4666 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4659 
                     /label="GAK"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGCTGCTG CAGTCGGCGC TCGACTTCTT GGCGGGTCCA GGCTCCCTGG 
     1081 GCGGTGCTTC CGGCCGCGAC CAGAGTGACT TCGTGGGGCA GACGGTGGAA CTGGGCGAGC 
     1141 TGCGGCTGCG GGTGCGGCGG GTCCTGGCCG AAGGGCAGCT GGTGGAATTT TTGAAGAAAA 
     1201 TGGAATCTCG AGGCCCCCTT TCGTGCGACA CGGTTCTGAA GATCTTCTAC CAGACGTGCC 
     1261 GCGCCGTGCA GCACATGCAC CGGCAGAAGC CGCCCATCAT CCACAGGGAC CTCAAGGTTG 
     1321 AGAACTTGTT GCTTAGTAAC CAAGGGACCA TTAAGCTGTG TGACTTTGGC AGTGCCACGA 
     1381 CCATCTCGCA CTACCCTGAC TACAGCTGGA GCGCCCAGAG GCGAGCCCTG GTGGAGGAAG 
     1441 AGATCACGAG GAATACAACA CCAATGTATA GAACACCAGA AATCATAGAC TTGTATTCCA 
     1501 ACTTCCCGAT CGGCGAGAAG CAGGATATCT GGGCCCTGGG CTGCATCTTG TACCTGCTGT 
     1561 GCTTCCGGCA GCACCCTTTT GAGGATGGAG CGAAACTTCG AATAGTCAAT GGCGCCATGC 
     1621 TGCAGGTGAA CCCGGAGGAG CGGCTGTCCA TCGCCGAGGT GGTGCACCAG CTGCAGGAGA 
     1681 TCGCGGCCGC CCGCAACGTG AACCCCAAGT CTCCCATCAC AGAGCTCCTG GAGCAGAATG 
     1741 GAGGCTACGG GAGCGCCACA CTGTCCCGAG GGCCACCCCC TCCCGTGGGC CCCGCTGGCA 
     1801 GTGGCTACAG TGGAGGCCTG GCGCTGGCGG AGTACGACCA GCCGTATGGC GGCTTCCTGG 
     1861 ACATTCTGCG GGGTGGGACA GAGCGGCTCT TCACCAACCT CAAGGACACC TCCTCCAAGG 
     1921 TCATCCAGTC CGTCGCTAAT TATGCAAAGG GTGACCTGGA CATATCTTAC ATCACATCCA 
     1981 GAATTGCAGT GATGTCATTC CCAGCAGAAG GTGTGGAGTC AGCGCTCAAA AACAACATCG 
     2041 AAGATGTGCG GTTGTTCCTG GACTCCAAGC ACCCAGGGCA CTATGCCGTC TACAACCTGT 
     2101 CCCCGAGGAC CTACCGGCCC TCCAGGTTCC ACAACCGGGT CTCCGAGTGT GGCTGGGCAG 
     2161 CACGGCGGGC CCCACACCTG CACACCCTGT ACAACATCTG CAGGAACATG CACGCCTGGC 
     2221 TGCGGCAGGA CCACAAGAAC GTCTGCGTCG TGCACTGCAT GGACGGGAGA GCCGCGTCTG 
     2281 CTGTGGCCGT CTGCTCCTTC CTGTGCTTCT GCCGTCTCTT CAGCACCGCG GAGGCCGCCG 
     2341 TGTACATGTT CAGCATGAAG CGCTGCCCAC CAGGCATCTG GCCATCCCAC AAAAGGTACA 
     2401 TCGAGTACAT GTGTGACATG GTGGCGGAGG AGCCCATCAC ACCCCACAGC AAGCCCATCC 
     2461 TGGTGAGGGC CGTGGTCATG ACACCCGTGC CGCTGTTCAG CAAGCAGAGG AGCGGCTGCA 
     2521 GGCCCTTCTG CGAGGTCTAC GTGGGGGACG AGCGTGTGGC CAGCACCTCC CAGGAGTACG 
     2581 ACAAGATGCG GGACTTTAAG ATTGAAGATG GCAAAGCGGT GATTCCCCTG GGCGTCACGG 
     2641 TGCAAGGAGA CGTGCTCATC GTCATCTATC ACGCCCGGTC CACTCTGGGC GGCCGGCTGC 
     2701 AGGCCAAGAT GGCATCCATG AAGATGTTCC AGATTCAGTT CCACACGGGG TTTGTGCCTC 
     2761 GGAACGCCAC CACTGTGAAA TTTGCCAAGT ATGACCTGGA CGCGTGTGAC ATTCAAGAAA 
     2821 AATACCCGGA TTTATTTCAA GTGAACCTGG AAGTGGAGGT GGAGCCCAGG GACAGGCCGA 
     2881 GCCGGGAAGC CCCACCATGG GAGAACTCGA GCATGAGGGG GCTGAACCCC AAAATCCTGT 
     2941 TTTCCAGCCG GGAGGAGCAG CAAGACATTC TGTCTAAGTT TGGGAAGCCG GAGCTTCCCC 
     3001 GGCAGCCTGG CTCCACGGCT CAGTATGATG CTGGGGCAGG GTCCCCGGAA GCCGAACCCA 
     3061 CAGACTCTGA CTCACCGCCA AGCAGCAGCG CGGACGCCAG TCGCTTCCTG CACACGCTGG 
     3121 ACTGGCAGGA AGAGAAGGAG GCAGAGACTG GTGCAGAAAA TGCCTCTTCC AAGGAGAGCG 
     3181 AGTCTGCCCT GATGGAGGAC AGAGACGAGA GTGAGGTGTC AGATGAAGGG GGATCCCCGA 
     3241 TCTCCAGCGA GGGCCAGGAA CCCAGGGCCG ACCCAGAGCC CCCCGGCCTG GCAGCAGGGC 
     3301 TGGTGCAGCA GGACTTGGTT TTTGAGGTGG AGACACCGGC TGTGCTGCCA GAGCCTGTGC 
     3361 CACAGGAAGA CGGGGTCGAC CTCCTGGGCC TGCACTCCGA GGTGGGCGCA GGGCCAGCTG 
     3421 TACCCCCGCA GGCCTGCAAG GCCCCCTCCA GCAACACCGA CCTGCTCAGC TGCCTCCTTG 
     3481 GGCCCCCTGA GGCCGCCTCC CAGGGGCCCC CGGAGGATCT GCTCAGCGAG GACCCGCTGC 
     3541 TCCTGGCAAG CCCGGCCCCT CCCCTGAGCG TGCAGAGCAC CCCAAGAGGA GGGCCCCCTG 
     3601 CCGCTGCTGA CCCCTTTGGC CCGCTTCTGC CGTCTTCAGG CAACAACTCC CAGCCCTGCT 
     3661 CCAATCCTGA TCTCTTCGGC GAATTTCTCA ATTCGGACTC TGTGACCGTC CCACCATCCT 
     3721 TCCCGTCTGC CCACAGTGCT CCGCCCCCAT CCTGCAGCGC CGACTTCCTG CACCTGGGGG 
     3781 ATCTGCCAGG AGAGCCCAGC AAGATGACAG CCTCGTCCAG CAACCCAGAC CTGCTGGGAG 
     3841 GATGGGCTGC CTGGACCGAG ACTGCAGCAT CGGCAGTGGC CCCCACGCCA GCCACAGAAG 
     3901 GCCCCCTCTT CTCTCCTGGA GGTCAGCCGG CCCCTTGTGG CTCTCAGGCC AGCTGGACCA 
     3961 AGTCTCAGAA CCCGGACCCA TTTGCTGACC TTGGCGACCT CAGCTCCGGC CTCCAAGGCT 
     4021 CACCAGCTGG ATTCCCTCCT GGGGGCTTCA TTCCCAAAAC GGCCACCACG CCCAAAGGCA 
     4081 GCAGCTCCTG GCAGACAAGT CGGCCGCCAG CCCAGGGCGC CTCATGGCCC CCTCAGGCCA 
     4141 AGCCGCCCCC CAAAGCCTGC ACACAGCCAA GGCCTAACTA TGCCTCGAAC TTCAGTGTGA 
     4201 TCGGGGCGCG GGAGGAGCGG GGGGTCCGCG CACCCAGCTT TGCTCAAAAG CCAAAAGTCT 
     4261 CTGAGAACGA CTTTGAAGAT CTGTTGTCCA ATCAAGGCTT CTCCTCCAGG TCTGACAAGA 
     4321 AAGGGCCAAA GACCATTGCA GAGATGAGGA AGCAGGACCT GGCTAAAGAC ACGGACCCAC 
     4381 TCAAGCTGAA GCTCCTGGAC TGGATTGAGG GCAAGGAGCG GAACATCCGG GCCCTGCTGT 
     4441 CCACGCTGCA CACAGTGCTG TGGGACGGGG AGAGCCGCTG GACGCCCGTG GGCATGGCCG 
     4501 ACCTGGTGGC TCCGGAGCAA GTGAAGAAGC ACTATCGCCG CGCGGTGCTG GCTGTGCACC 
     4561 CCGACAAGGC TGCGGGGCAG CCGTACGAGC AGCACGCCAA GATGATCTTC ATGGAGCTGA 
     4621 ATGACGCCTG GTCGGAGTTT GAGAACCAGG GCTCCCGGCC CCTCTTCAGC GGACCGACGC 
     4681 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4741 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4801 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4861 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4921 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4981 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5041 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5101 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5161 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5221 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5281 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5341 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5401 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5461 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5521 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5581 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5641 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5701 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5761 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5821 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5881 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5941 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6001 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6061 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6121 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6181 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6241 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6301 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6361 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6421 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6481 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6541 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6601 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6661 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6721 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6781 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6841 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6901 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6961 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7021 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7081 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7141 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7201 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7261 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7321 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7381 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7441 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7501 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7561 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7621 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7681 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7741 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7801 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7861 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7921 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7981 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8041 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8101 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8161 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8221 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8281 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8341 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8401 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8461 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8521 GCGAATTTT
//